Reviewed, UniProtKB/Swiss-Prot Q8YEX2 (CYSD_BRUME)

Names and origin

Protein names

Recommended name:
    Sulfate adenylyltransferase subunit 2
    EC=2.7.7.4
Alternative name(s):
    Sulfate adenylate transferase
      Short name=SAT
    ATP-sulfurylase small subunit

Gene names

Name:	cysD
Ordered Locus Names:	BMEI1755

Organism

Brucella melitensis [Complete proteome] [HAMAP]

Taxonomic identifier

29459 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Alphaproteobacteria › Rhizobiales › Brucellaceae › Brucella

Protein attributes

Sequence length	300 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Inferred from homology.

General annotation (Comments)

Catalytic activity	ATP + sulfate = diphosphate + adenylyl sulfate. HAMAP MF_00064
Pathway	Sulfur metabolism; hydrogen sulfide biosynthesis; sulfite from sulfate: step 1/3. HAMAP MF_00064
Subunit structure	Heterodimer composed of cysD, the smaller subunit, and cysN By similarity.
Sequence similarities	Belongs to the PAPS reductase family. CysD subfamily.

Ontologies

Keywords
Ligand	ATP-binding Nucleotide-binding
Molecular function	Nucleotidyltransferase Transferase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	sulfate assimilation Inferred from electronic annotation. Source: HAMAP sulfate reduction Inferred from electronic annotation. Source: InterPro
Molecular function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW sulfate adenylyltransferase (ATP) activity Inferred from electronic annotation. Source: HAMAP
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 300	300	Sulfate adenylyltransferase subunit 2 HAMAP MF_00064		PRO_0000340185

Sequences

	Sequence		Length	Mass (Da)
	Q8YEX2-1 [UniParc]. Last modified June 10, 2008. Version 2. Checksum: A35926DA9DAF83BA	FASTA	300	34,742
	10 20 30 40 50 60 MKNLTHLQRL EAEAIHVFRE VAATFSNPVM LYSVSKDSSV MLHLAMKAFY PAPPPFPFLH 70 80 90 100 110 120 VDTTWKFREM IEFRDAQARE KGFELLVHVN EQGVRDGIGP FTHGSNVHTH IMKTVGLRQA 130 140 150 160 170 180 LDKYRFDAAF GGARRDEEKS RAKERIFSFR NAQHGWDPKN QRPEMWKIYN TRVSKGESIR 190 200 210 220 230 240 VFPLSNWTEL DIWQYILQEN IPIVPLYFAA RRPVVERDGM LIMVDDDRMK LRPGEPVENR 250 260 270 280 290 300 LVRFRTLGCY PLTGAIPSSA ANLSDIVEEM LIARTSERQG RAIDRDEAGS MEKKKREGYF

References

[1]

"The genome sequence of the facultative intracellular pathogen Brucella melitensis." DelVecchio V.G., Kapatral V., Redkar R.J., Patra G., Mujer C., Los T., Ivanova N., Anderson I., Bhattacharyya A., Lykidis A., Reznik G., Jablonski L., Larsen N., D'Souza M., Bernal A., Mazur M., Goltsman E., Selkov E. , Elzer P.H., Hagius S., O'Callaghan D., Letesson J.-J., Haselkorn R., Kyrpides N.C., Overbeek R. Proc. Natl. Acad. Sci. U.S.A. 99:443-448(2002) [PubMed: 11756688] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: 16M / ATCC 23456 / NCTC 10094 / Biotype 1.

Cross-references

Sequence databases
EMBLGenBankDDBJ	AE008917 Genomic DNA. Translation: AAL52936.1. Different initiation.
PIR	AE3471.
RefSeq	NP_540672.2.
3D structure databases
SMR	Q8YEX2. Positions 29-241.
ModBase	Search...
Genome annotation databases
GeneID	1197466.
GenomeReviews	Gene locus BMEI1755 in contig AE008917_GR.
KEGG	bme:BMEI1755.
NMPDR	fig|224914.1.peg.1754.
Organism-specific databases
CMR	Search...
Phylogenomic databases
HOGENOM	Q8YEX2.
Enzyme and pathway databases
BioCyc	BMEL224914:BMEI1755-MON.
Family and domain databases
HAMAP	MF_00064. [Tree]
InterPro	IPR002500. PAPS_reduct. IPR014729. Rossmann-like_a/b/a_fold. IPR011784. SO4_adenylTrfase_ssu. [Graphical view]
Gene3D	G3DSA:3.40.50.620. Rossmann-like_a/b/a_fold. 1 hit.
Pfam	PF01507. PAPS_reduct. 1 hit. [Graphical view]
PIRSF	PIRSF002936. CysDAde_trans. 1 hit.
TIGRFAMs	TIGR02039. CysD. 1 hit.
ProtoNet	Search...

Entry information

Entry name

CYSD_BRUME

Accession

Primary (citable) accession number: Q8YEX2

Entry history

Integrated into UniProtKB/Swiss-Prot:	June 10, 2008
Last sequence update:	June 10, 2008
Last modified:	October 13, 2009
This is version 44 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)