Reviewed, UniProtKB/Swiss-Prot Q8YES3 (GLND_BRUME)

Names and origin

Protein names

Recommended name:
    [Protein-PII] uridylyltransferase
      Short name=PII uridylyl-transferase
    EC=2.7.7.59
Alternative name(s):
    Uridylyl-removing enzyme
    UTase

Gene names

Name:	glnD
Ordered Locus Names:	BMEI1804

Organism

Brucella melitensis [Complete proteome] [HAMAP]

Taxonomic identifier

29459 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Alphaproteobacteria › Rhizobiales › Brucellaceae › Brucella

Protein attributes

Sequence length	934 AA.
Sequence status	Complete.
Protein existence	Inferred from homology.

General annotation (Comments)

Function	Modifies, by uridylylation or deuridylylation the PII (glnB) regulatory protein By similarity. HAMAP MF_00277
Catalytic activity	UTP + [protein-PII] = diphosphate + uridylyl-[protein-PII]. HAMAP MF_00277
Sequence similarities	Belongs to the glnD family.

Ontologies

Keywords
Molecular function	Nucleotidyltransferase Transferase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	nitrogen compound metabolic process Inferred from electronic annotation. Source: InterPro
Molecular function	[protein-PII] uridylyltransferase activity Inferred from electronic annotation. Source: HAMAP amino acid binding Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 934	934	[Protein-PII] uridylyltransferase HAMAP MF_00277		PRO_0000192723

Sequences

	Sequence		Length	Mass (Da)
	Q8YES3-1 [UniParc]. Last modified March 1, 2002. Version 1. Checksum: 74F9987863AE3BFE	FASTA	934	105,930
	10 20 30 40 50 60 MSAHDLKLEE IVNAETLRRK LNELADTADE SYTSLPMRKV VLQTLKDALA SGRANAEDML 70 80 90 100 110 120 MKDGGGTLCA KRLCYLMDTL IDILFEFATT RAYPTRNPSK AENMALVAVG GYGRGGLAQG 130 140 150 160 170 180 SDIDLLFLLP YKQTPWGEQV VEYTLYMLWD MGLKVGHSTR NIDECIRLAR EDMTIRTALL 190 200 210 220 230 240 DARFLTGDKD LFRTLEIRFE EEIVKGTEPE FIQAKLAERD ARHRKAGETR YLVEPNVKEG 250 260 270 280 290 300 KGGQRDLHTL FWITKYFYRV KTKEELVKLG VLSRAELKLF NKAEDFLWAV RCHMHFATLK 310 320 330 340 350 360 AEERLSFDIQ PEIAQRLGYT AHPGQNYVER FMKHYFLVAK DVGDLTRIIC AALEEQQAKH 370 380 390 400 410 420 VPGFNRIFLT FSRRKRKLSD DGAFISENHR INIARPDIFR QDPVNMIRLF HLADRHGLEF 430 440 450 460 470 480 HPEAMQSLTR SLKLINADLR ENPEANRLFL EILTSPRNPE LILRRMNESG VLGKFIPDFG 490 500 510 520 530 540 KIVAMMQFNM YHHYTVDEHL LRCIAVLSEI EHGELKTEHP LSNHLITTIK RDRNLLYVTL 550 560 570 580 590 600 LLHDIAKGRP EDHSIAGARI ARRLCPRFGL TPSETETVEW LVREHLTMSM VAQSRDLNDR 610 620 630 640 650 660 KTIIDFADTV QTMERLKLLL ILTVCDIKAV GPGIWNGWKG QLLRTLFYET ELVLTGGFSE 670 680 690 700 710 720 LSRAARDKQA REALAERLSD WPKEERDAYL ALPYTNYFLT VSLDDQVRHA HFIRDADQQG 730 740 750 760 770 780 RALVTMAKPH AFEAVTEITV LAPDHPRLLS VITGACAAAG GNIVDAQIFT TSDGRALDTI 790 800 810 820 830 840 LISREFDTDD DERRRAERVG KVIEDVLSGK AHLPDMLAKR TKPKKAARAF KVEPRVEINN 850 860 870 880 890 900 TLSNKFTVIE VEGLDRPGLL SELTGLISDL SLDIASAHIT TFGEKVIDSF YVTDLVGHKI 910 920 930 SNATRQGNIK RKLLALLGAE NGARTNGRSP QAAA

References

[1]

"The genome sequence of the facultative intracellular pathogen Brucella melitensis." DelVecchio V.G., Kapatral V., Redkar R.J., Patra G., Mujer C., Los T., Ivanova N., Anderson I., Bhattacharyya A., Lykidis A., Reznik G., Jablonski L., Larsen N., D'Souza M., Bernal A., Mazur M., Goltsman E., Selkov E. , Elzer P.H., Hagius S., O'Callaghan D., Letesson J.-J., Haselkorn R., Kyrpides N.C., Overbeek R. Proc. Natl. Acad. Sci. U.S.A. 99:443-448(2002) [PubMed: 11756688] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: 16M / ATCC 23456 / NCTC 10094 / Biotype 1.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AE008917 Genomic DNA. Translation: AAL52985.1.
PIR	AF3477.
RefSeq	NP_540721.1.
3D structure databases
SMR	Q8YES3. Positions 70-343, 387-578, 737-893.
ModBase	Search...
Genome annotation databases
GeneID	1197515.
GenomeReviews	Gene locus BMEI1804 in contig AE008917_GR.
KEGG	bme:BMEI1804.
Organism-specific databases
CMR	Search...
Phylogenomic databases
HOGENOM	HBG687872.
OMA	MQHDLFH.
PhylomeDB	Q8YES3.
Enzyme and pathway databases
BioCyc	BMEL224914:BMEI1804-MONOMER.
BRENDA	2.7.7.59. 277959.
Family and domain databases
HAMAP	MF_00277. PII_uridylyl-transf. [Tree]
InterPro	IPR002912. ACT_bd. IPR010043. GlnD_Uridyltrans. IPR003607. Metal-dep_PHydrolase_HD_dom. IPR006674. Metal-dep_PHydrolase_HD_sub. IPR002934. Nucleotidyltransferase. IPR013546. PII_UdlTrfase/GS_AdlTrfase. [Graphical view]
PANTHER	PTHR13734:SF1. GlnD_Uridyltrans. 1 hit.
Pfam	PF01842. ACT. 2 hits. PF08335. GlnD_UR_UTase. 1 hit. PF01966. HD. 1 hit. PF01909. NTP_transf_2. 1 hit. [Graphical view]
PIRSF	PIRSF006288. PII_uridyltransf. 1 hit.
SMART	SM00471. HDc. 1 hit. [Graphical view]
TIGRFAMs	TIGR01693. UTase_glnD. 1 hit.
ProtoNet	Search...

Entry information

Entry name

GLND_BRUME

Accession

Primary (citable) accession number: Q8YES3

Entry history

Integrated into UniProtKB/Swiss-Prot:	August 13, 2002
Last sequence update:	March 1, 2002
Last modified:	February 9, 2010
This is version 50 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)