Reviewed, UniProtKB/Swiss-Prot Q8YEK8 (ASSY_BRUME)

Names and origin

Protein names

Recommended name:
    Argininosuccinate synthase
    EC=6.3.4.5
Alternative name(s):
    Citrulline--aspartate ligase

Gene names

Name:	argG
Ordered Locus Names:	BMEI1870

Organism

Brucella melitensis [Complete proteome] [HAMAP]

Taxonomic identifier

29459 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Alphaproteobacteria › Rhizobiales › Brucellaceae › Brucella

Protein attributes

Sequence length	406 AA.
Sequence status	Complete.
Protein existence	Inferred from homology.

General annotation (Comments)

Catalytic activity	ATP + L-citrulline + L-aspartate = AMP + diphosphate + N(omega)-(L-arginino)succinate. HAMAP MF_00005
Pathway	Amino-acid biosynthesis; L-arginine biosynthesis; L-arginine from L-ornithine and carbamoyl phosphate: step 2/3. HAMAP MF_00005
Subunit structure	Homotetramer By similarity. HAMAP MF_00005
Subcellular location	Cytoplasm Probable HAMAP MF_00005.
Sequence similarities	Belongs to the argininosuccinate synthase family. Type 1 subfamily.

Ontologies

Keywords
Biological process	Amino-acid biosynthesis Arginine biosynthesis
Cellular component	Cytoplasm
Ligand	ATP-binding Nucleotide-binding
Molecular function	Ligase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	arginine biosynthetic process Inferred from electronic annotation. Source: HAMAP
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	ATP binding Inferred from electronic annotation. Source: HAMAP argininosuccinate synthase activity Inferred from electronic annotation. Source: HAMAP
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 406	406	Argininosuccinate synthase HAMAP MF_00005		PRO_0000148574
Regions
	Nucleotide binding	13 – 21	9	ATP By similarity
Sites
	Binding site	40	1	ATP; via amide nitrogen and carbonyl oxygen By similarity
	Binding site	91	1	Citrulline By similarity
	Binding site	96	1	Citrulline By similarity
	Binding site	121	1	ATP; via amide nitrogen By similarity
	Binding site	123	1	Aspartate By similarity
	Binding site	127	1	Aspartate By similarity
	Binding site	127	1	Citrulline By similarity
	Binding site	128	1	Aspartate By similarity
	Binding site	131	1	Citrulline By similarity
	Binding site	182	1	Citrulline By similarity
	Binding site	191	1	Citrulline By similarity
	Binding site	267	1	Citrulline By similarity
	Binding site	279	1	Citrulline By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q8YEK8-1 [UniParc]. Last modified March 1, 2002. Version 1. Checksum: 534F07859F1B8F7D	FASTA	406	45,273
	10 20 30 40 50 60 MSKWKDVKKV VLAYSGGLDT SIILKWLQTE LGAEVVTFTA DLGQGEELEP ARKKAEMLGI 70 80 90 100 110 120 KEIFIEDVRE EFVRDFVFPM FRANAVYEGV YLLGTSIARP LISKHLIDIA KKTGADAIAH 130 140 150 160 170 180 GATGKGNDQV RFELSAYALN PDIKIIAPWR DWSFKSRTQL LEFAEQHQIP VAKDKKGEAP 190 200 210 220 230 240 FSVDANLLHS SSEGKVLEDP SQEAPEYVHM RTISPETAPD KATIIKIGFE KGDAVSINGE 250 260 270 280 290 300 RLSPATLLAK LNDYGRDNGI GRLDLVENRF VGMKSRGVYE TPGGTILLAA HRAIESITLD 310 320 330 340 350 360 RGAAHLKDEL MPRYAELIYY GFWFSPEREM LQAAIDHSQR HVEGEVTLKL YKGNVMVIGR 370 380 390 400 ESAKSLYSDK LVTFEDDQGA YDQKDAAGFI KLNALRLRTL AARDRK

References

[1]

"The genome sequence of the facultative intracellular pathogen Brucella melitensis." DelVecchio V.G., Kapatral V., Redkar R.J., Patra G., Mujer C., Los T., Ivanova N., Anderson I., Bhattacharyya A., Lykidis A., Reznik G., Jablonski L., Larsen N., D'Souza M., Bernal A., Mazur M., Goltsman E., Selkov E. , Elzer P.H., Hagius S., O'Callaghan D., Letesson J.-J., Haselkorn R., Kyrpides N.C., Overbeek R. Proc. Natl. Acad. Sci. U.S.A. 99:443-448(2002) [PubMed: 11756688] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: 16M / ATCC 23456 / NCTC 10094 / Biotype 1.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AE008917 Genomic DNA. Translation: AAL53051.1.
PIR	AH3485.
RefSeq	NP_540787.1.
3D structure databases
SMR	Q8YEK8. Positions 9-405.
ModBase	Search...
Genome annotation databases
GeneID	1197581.
GenomeReviews	Gene locus BMEI1870 in contig AE008917_GR.
KEGG	bme:BMEI1870.
Organism-specific databases
CMR	Search...
Phylogenomic databases
HOGENOM	HBG335267.
OMA	PLIAKRQ.
PhylomeDB	Q8YEK8.
Enzyme and pathway databases
BioCyc	BMEL224914:BMEI1870-MONOMER.
BRENDA	6.3.4.5. 277959.
Family and domain databases
HAMAP	MF_00005. Arg_succ_synth_type1. [Tree]
InterPro	IPR001518. Arginosuc_synth. IPR018223. Arginosuc_synth_CS. IPR014729. Rossmann-like_a/b/a_fold. [Graphical view]
Gene3D	G3DSA:3.40.50.620. Rossmann-like_a/b/a_fold. 1 hit.
PANTHER	PTHR11587. Arginosuc_synth. 1 hit.
Pfam	PF00764. Arginosuc_synth. 1 hit. [Graphical view]
TIGRFAMs	TIGR00032. argG. 1 hit.
PROSITE	PS00564. ARGININOSUCCIN_SYN_1. 1 hit. PS00565. ARGININOSUCCIN_SYN_2. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

ASSY_BRUME

Accession

Primary (citable) accession number: Q8YEK8

Entry history

Integrated into UniProtKB/Swiss-Prot:	October 19, 2002
Last sequence update:	March 1, 2002
Last modified:	February 9, 2010
This is version 53 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)