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Q8YEK8

- ASSY_BRUME

UniProt

Q8YEK8 - ASSY_BRUME

Protein

Argininosuccinate synthase

Gene

argG

Organism
Brucella melitensis biotype 1 (strain 16M / ATCC 23456 / NCTC 10094)
Status
Reviewed - Annotation score: 3 out of 5- Protein inferred from homologyi
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    • History
      Entry version 80 (01 Oct 2014)
      Sequence version 1 (01 Mar 2002)
      Previous versions | rss
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    Functioni

    Catalytic activityi

    ATP + L-citrulline + L-aspartate = AMP + diphosphate + N(omega)-(L-arginino)succinate.UniRule annotation

    Pathwayi

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Binding sitei40 – 401ATP; via amide nitrogen and carbonyl oxygenUniRule annotation
    Binding sitei91 – 911CitrullineUniRule annotation
    Binding sitei96 – 961CitrullineUniRule annotation
    Binding sitei121 – 1211ATP; via amide nitrogenUniRule annotation
    Binding sitei123 – 1231AspartateUniRule annotation
    Binding sitei127 – 1271AspartateUniRule annotation
    Binding sitei127 – 1271CitrullineUniRule annotation
    Binding sitei128 – 1281AspartateUniRule annotation
    Binding sitei131 – 1311CitrullineUniRule annotation
    Binding sitei182 – 1821CitrullineUniRule annotation
    Binding sitei191 – 1911CitrullineUniRule annotation
    Binding sitei267 – 2671CitrullineUniRule annotation
    Binding sitei279 – 2791CitrullineUniRule annotation

    Regions

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Nucleotide bindingi13 – 219ATPUniRule annotation

    GO - Molecular functioni

    1. argininosuccinate synthase activity Source: UniProtKB-HAMAP
    2. ATP binding Source: UniProtKB-HAMAP

    GO - Biological processi

    1. arginine biosynthetic process Source: UniProtKB-HAMAP

    Keywords - Molecular functioni

    Ligase

    Keywords - Biological processi

    Amino-acid biosynthesis, Arginine biosynthesis

    Keywords - Ligandi

    ATP-binding, Nucleotide-binding

    Enzyme and pathway databases

    UniPathwayiUPA00068; UER00113.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Argininosuccinate synthaseUniRule annotation (EC:6.3.4.5UniRule annotation)
    Alternative name(s):
    Citrulline--aspartate ligaseUniRule annotation
    Gene namesi
    Name:argGUniRule annotation
    Ordered Locus Names:BMEI1870
    OrganismiBrucella melitensis biotype 1 (strain 16M / ATCC 23456 / NCTC 10094)
    Taxonomic identifieri224914 [NCBI]
    Taxonomic lineageiBacteriaProteobacteriaAlphaproteobacteriaRhizobialesBrucellaceaeBrucella
    ProteomesiUP000000419: Chromosome I

    Subcellular locationi

    Cytoplasm UniRule annotation

    GO - Cellular componenti

    1. cytoplasm Source: UniProtKB-SubCell

    Keywords - Cellular componenti

    Cytoplasm

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 406406Argininosuccinate synthasePRO_0000148574Add
    BLAST

    Proteomic databases

    PRIDEiQ8YEK8.

    Interactioni

    Subunit structurei

    Homotetramer.UniRule annotation

    Protein-protein interaction databases

    STRINGi224914.BMEI1870.

    Structurei

    3D structure databases

    ProteinModelPortaliQ8YEK8.
    SMRiQ8YEK8. Positions 9-405.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Sequence similaritiesi

    Belongs to the argininosuccinate synthase family. Type 1 subfamily.UniRule annotation

    Phylogenomic databases

    HOGENOMiHOG000230093.
    KOiK01940.
    OMAiAPPEEAY.
    OrthoDBiEOG6K9QCV.

    Family and domain databases

    Gene3Di3.40.50.620. 1 hit.
    3.90.1260.10. 1 hit.
    HAMAPiMF_00005. Arg_succ_synth_type1.
    InterProiIPR001518. Arginosuc_synth.
    IPR018223. Arginosuc_synth_CS.
    IPR023434. Arginosuc_synth_type_1_subfam.
    IPR024074. AS_cat/multimer_dom_body.
    IPR014729. Rossmann-like_a/b/a_fold.
    [Graphical view]
    PfamiPF00764. Arginosuc_synth. 1 hit.
    [Graphical view]
    TIGRFAMsiTIGR00032. argG. 1 hit.
    PROSITEiPS00564. ARGININOSUCCIN_SYN_1. 1 hit.
    PS00565. ARGININOSUCCIN_SYN_2. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    Q8YEK8-1 [UniParc]FASTAAdd to Basket

    « Hide

    MSKWKDVKKV VLAYSGGLDT SIILKWLQTE LGAEVVTFTA DLGQGEELEP    50
    ARKKAEMLGI KEIFIEDVRE EFVRDFVFPM FRANAVYEGV YLLGTSIARP 100
    LISKHLIDIA KKTGADAIAH GATGKGNDQV RFELSAYALN PDIKIIAPWR 150
    DWSFKSRTQL LEFAEQHQIP VAKDKKGEAP FSVDANLLHS SSEGKVLEDP 200
    SQEAPEYVHM RTISPETAPD KATIIKIGFE KGDAVSINGE RLSPATLLAK 250
    LNDYGRDNGI GRLDLVENRF VGMKSRGVYE TPGGTILLAA HRAIESITLD 300
    RGAAHLKDEL MPRYAELIYY GFWFSPEREM LQAAIDHSQR HVEGEVTLKL 350
    YKGNVMVIGR ESAKSLYSDK LVTFEDDQGA YDQKDAAGFI KLNALRLRTL 400
    AARDRK 406
    Length:406
    Mass (Da):45,273
    Last modified:March 1, 2002 - v1
    Checksum:i534F07859F1B8F7D
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AE008917 Genomic DNA. Translation: AAL53051.1.
    PIRiAH3485.
    RefSeqiNP_540787.1. NC_003317.1.

    Genome annotation databases

    EnsemblBacteriaiAAL53051; AAL53051; BMEI1870.
    GeneIDi1197581.
    KEGGibme:BMEI1870.
    PATRICi17796138. VBIBruMel146950_1341.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AE008917 Genomic DNA. Translation: AAL53051.1 .
    PIRi AH3485.
    RefSeqi NP_540787.1. NC_003317.1.

    3D structure databases

    ProteinModelPortali Q8YEK8.
    SMRi Q8YEK8. Positions 9-405.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    STRINGi 224914.BMEI1870.

    Proteomic databases

    PRIDEi Q8YEK8.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblBacteriai AAL53051 ; AAL53051 ; BMEI1870 .
    GeneIDi 1197581.
    KEGGi bme:BMEI1870.
    PATRICi 17796138. VBIBruMel146950_1341.

    Phylogenomic databases

    HOGENOMi HOG000230093.
    KOi K01940.
    OMAi APPEEAY.
    OrthoDBi EOG6K9QCV.

    Enzyme and pathway databases

    UniPathwayi UPA00068 ; UER00113 .

    Family and domain databases

    Gene3Di 3.40.50.620. 1 hit.
    3.90.1260.10. 1 hit.
    HAMAPi MF_00005. Arg_succ_synth_type1.
    InterProi IPR001518. Arginosuc_synth.
    IPR018223. Arginosuc_synth_CS.
    IPR023434. Arginosuc_synth_type_1_subfam.
    IPR024074. AS_cat/multimer_dom_body.
    IPR014729. Rossmann-like_a/b/a_fold.
    [Graphical view ]
    Pfami PF00764. Arginosuc_synth. 1 hit.
    [Graphical view ]
    TIGRFAMsi TIGR00032. argG. 1 hit.
    PROSITEi PS00564. ARGININOSUCCIN_SYN_1. 1 hit.
    PS00565. ARGININOSUCCIN_SYN_2. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: 16M / ATCC 23456 / NCTC 10094.

    Entry informationi

    Entry nameiASSY_BRUME
    AccessioniPrimary (citable) accession number: Q8YEK8
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: October 19, 2002
    Last sequence update: March 1, 2002
    Last modified: October 1, 2014
    This is version 80 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programProkaryotic Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome

    Documents

    1. Brucella melitensis
      Brucella melitensis (strain 16M): entries and gene names
    2. PATHWAY comments
      Index of metabolic and biosynthesis pathways
    3. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3