Q8YEK2 (DNAE2_BRUME) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Error-prone DNA polymerase
EC=2.7.7.7

Gene names

Name:	dnaE2
Ordered Locus Names:	BMEI1876

Organism

Brucella melitensis biotype 1 (strain 16M / ATCC 23456 / NCTC 10094) [Complete proteome] [HAMAP]

Taxonomic identifier

224914 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Alphaproteobacteria › Rhizobiales › Brucellaceae › Brucella

Protein attributes

Sequence length	1077 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	DNA polymerase involved in damage-induced mutagenesis and translesion synthesis (TLS). It is not the major replicative DNA polymerase By similarity. HAMAP MF_01902
Catalytic activity	Deoxynucleoside triphosphate + DNA(n) = diphosphate + DNA(n+1). HAMAP MF_01902
Subcellular location	Cytoplasm By similarity HAMAP MF_01902.
Sequence similarities	Belongs to the DNA polymerase type-C family. DnaE2 subfamily.
Sequence caution	The sequence AAL53057.1 differs from that shown. Reason: Erroneous initiation.

Ontologies

Keywords
Biological process	DNA damage DNA repair DNA replication
Cellular component	Cytoplasm
Molecular function	DNA-directed DNA polymerase Nucleotidyltransferase Transferase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	DNA repair Inferred from electronic annotation. Source: UniProtKB-KW DNA replication Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	3'-5' exonuclease activity Inferred from electronic annotation. Source: InterPro DNA binding Inferred from electronic annotation. Source: InterPro DNA-directed DNA polymerase activity Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 1077	1077	Error-prone DNA polymerase HAMAP MF_01902		PRO_0000103371

Sequences

	Sequence		Length	Mass (Da)
	Q8YEK2 [UniParc]. Last modified July 19, 2005. Version 2. Checksum: 1B13F29D15C68B26	FASTA	1,077	120,982
	10 20 30 40 50 60 MVPYFEMAAA SNFSFLCGAS HPQELVERAH ALDLSGIGIA DRNTLAGVVR AHAQWKDIRK 70 80 90 100 110 120 ESGFRLFIGC RLSFIDGTPD MVVYPRDRAA YGQLCRLLTE GKHRAAIKGE CHLEWADLLF 130 140 150 160 170 180 RARQFQIAVF PPDEDEPDFA ARLTEIAQAA PGSVWLALTM PHQGQDGRRA ERIARFAAQA 190 200 210 220 230 240 GVPLIATNDV LYHHPDRRPL QDVLTATRHH TTVFAAGRLL EKNAERHLKP PHEMVRLFRD 250 260 270 280 290 300 YPEAIAATAD FVAPITFQLD ELKYAYPDEP IPPGKTAQQH LYDLVWEGAA RHYGADMIPP 310 320 330 340 350 360 KVQGLINKEL ALIARLEYEP YFLTVYDIVT HAREKGILCQ GRGSAANSVV CFCLGITGVN 370 380 390 400 410 420 PTQVDLLFER FISAERKEPP DIDVDFEHER REEVMQYVYD RYSRDRAAIV ATVISYRSRS 430 440 450 460 470 480 AIRDVGKALG LSEDVTAALA NTVWGLSGGG IDRQHIRQAG LDPDNPIIQR AVELAITLIG 490 500 510 520 530 540 FPRHLSQHVG GFVLTRDRLD ETVPIGPAAM DKRSFIEWDK DDIDEVGLMK VDVLSLGMLT 550 560 570 580 590 600 CIRKAFDLIH QHKPQLYGGE KLTLASLPRK DKAVYDMLCK GDSLGVFQVE SRAQMNMLPR 610 620 630 640 650 660 LRPQEFYDLV IEVAIVRPGP IQGDMVHPYL RRRSGQEPCT LPSPSPQHGP ANELQQILGK 670 680 690 700 710 720 TKGVPLFQEQ AMRIAMEAAK FTPEEANQLR RAMATFRKMG TIHTMEKKMI DGMVNRGYDR 730 740 750 760 770 780 TFAENCFNQI KGFGEYGFPE SHAASFAHLV YISAWLKCHH PEVFAAALLN SQPMGFYAPA 790 800 810 820 830 840 QIVRDAREHG VTVLPVDVNF SQWDNILEET PDVHLALRLG FRQIDGFSKR DTELLIADRQ 850 860 870 880 890 900 EPYRTIEDMH RRLRLDRRAF TLLADADAFG SLDIDRRAAL WAVRRLPNDE TLLLFRAAAA 910 920 930 940 950 960 SELAQEPRTK LPEMAASEHV IADYETTRLS LKGHPLQYLR EGLAAEGVST CRAVQEGADG 970 980 990 1000 1010 1020 RRMKVAGVVT VRQRPGSAKG VVFLTIEDET GIANIVIWPK IMKVFRREVM SARLIHIEGR 1030 1040 1050 1060 1070 IQRSLEGVVH LVAAKLQDRS AALIEMSGRE AQRLIAPSQM AHHPRNVRVM PNSRDFH

References

[1]

"The genome sequence of the facultative intracellular pathogen Brucella melitensis." DelVecchio V.G., Kapatral V., Redkar R.J., Patra G., Mujer C., Los T., Ivanova N., Anderson I., Bhattacharyya A., Lykidis A., Reznik G., Jablonski L., Larsen N., D'Souza M., Bernal A., Mazur M., Goltsman E., Selkov E. , Elzer P.H., Hagius S., O'Callaghan D., Letesson J.-J., Haselkorn R., Kyrpides N.C., Overbeek R. Proc. Natl. Acad. Sci. U.S.A. 99:443-448(2002) [PubMed: 11756688] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: 16M / ATCC 23456 / NCTC 10094.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AE008917 Genomic DNA. Translation: AAL53057.1. Different initiation.
PIR	AF3486.
RefSeq	NP_540793.1. NC_003317.1.
3D structure databases
ProteinModelPortal	Q8YEK2.
ModBase	Search...
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	1197587.
GenomeReviews	Gene locus BMEI1876 in contig AE008917_GR.
KEGG	bme:BMEI1876.
NMPDR	fig|224914.1.peg.1875.
PATRIC	17854178. VBIBruMel92729_2074.
Organism-specific databases
CMR	Search...
Phylogenomic databases
HOGENOM	HBG734490.
OMA	EQPAIRL.
ProtClustDB	PRK05672.
Enzyme and pathway databases
BioCyc	BMEL224914:BMEI1876-MONOMER.
Family and domain databases
HAMAP	MF_01902. DNApol_error_prone. [Tree]
InterPro	IPR011708. DNA_pol3_alpha. IPR023073. DNA_pol_error_prone. IPR012340. NA-bd_OB-fold. IPR004365. NA-bd_OB_tRNA-helicase. IPR004013. PHP_C. IPR003141. Pol/His_phosphatase_N. IPR016195. Pol/histidinol_Pase-like. IPR004805. PolC_alpha. [Graphical view]
Gene3D	G3DSA:2.40.50.140. OB_NA_bd_sub. 1 hit.
KO	K14162.
Pfam	PF07733. DNA_pol3_alpha. 1 hit. PF02811. PHP. 1 hit. PF01336. tRNA_anti. 1 hit. [Graphical view]
SMART	SM00481. POLIIIAc. 1 hit. [Graphical view]
SUPFAM	SSF89550. PHP-like. 1 hit.
TIGRFAMs	TIGR00594. Polc. 1 hit.
ProtoNet	Search...

Entry information

Entry name

DNAE2_BRUME

Accession

Primary (citable) accession number: Q8YEK2

Entry history

Integrated into UniProtKB/Swiss-Prot:	July 19, 2005
Last sequence update:	July 19, 2005
Last modified:	January 25, 2012
This is version 65 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program