Q8YEG1 (AROA_BRUME) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
3-phosphoshikimate 1-carboxyvinyltransferase
EC=2.5.1.19

Alternative name(s):
5-enolpyruvylshikimate-3-phosphate synthase
Short name=EPSP synthase
Short name=EPSPS

Gene names

Name:	aroA
Ordered Locus Names:	BMEI1917

Organism

Brucella melitensis biotype 1 (strain 16M / ATCC 23456 / NCTC 10094) [Complete proteome] [HAMAP]

Taxonomic identifier

224914 [NCBI]

Taxonomic lineage

cellular organisms › Bacteria › Proteobacteria › Alphaproteobacteria › Rhizobiales › Brucellaceae › Brucella › Brucella melitensis › Brucella melitensis bv. 1

Protein attributes

Sequence length	450 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Catalytic activity	Phosphoenolpyruvate + 3-phosphoshikimate = phosphate + 5-O-(1-carboxyvinyl)-3-phosphoshikimate. HAMAP-Rule MF_00210
Pathway	Metabolic intermediate biosynthesis; chorismate biosynthesis; chorismate from D-erythrose 4-phosphate and phosphoenolpyruvate: step 6/7. HAMAP-Rule MF_00210
Subunit structure	Monomer By similarity.
Subcellular location	Cytoplasm By similarity.
Sequence similarities	Belongs to the EPSP synthase family.
Sequence caution	The sequence AAL53098.1 differs from that shown. Reason: Erroneous initiation.

Ontologies

Keywords
Biological process	Amino-acid biosynthesis Aromatic amino acid biosynthesis
Cellular component	Cytoplasm
Molecular function	Transferase
Technical term	Complete proteome
Gene Ontology (GO)
Biological_process	aromatic amino acid family biosynthetic process Inferred from electronic annotation. Source: HAMAP chorismate biosynthetic process Inferred from electronic annotation. Source: UniProtKB-UniPathway
Cellular_component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular_function	3-phosphoshikimate 1-carboxyvinyltransferase activity Inferred from electronic annotation. Source: HAMAP
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 450	450	3-phosphoshikimate 1-carboxyvinyltransferase HAMAP-Rule MF_00210		PRO_0000088233

Sequences

	Sequence		Length	Mass (Da)
	Q8YEG1 [UniParc]. Last modified June 6, 2003. Version 2. Checksum: 931C4B483C162CB7	FASTA	450	47,207
	10 20 30 40 50 60 MSHSACPKPA TARHSQALTG EIRIPGDKSI SHRSFMFGGL ASGKTRITGL LEGEDVINTG 70 80 90 100 110 120 RAMQAMGARI RKEGDVWIIN GVGNGCLLQP EAPLDFGNAG TGARLTMGLV GTYDMKTSFI 130 140 150 160 170 180 GDASLSKRPM GRVLNPLREM GVQVEAAEGD RMPLTLIGPR TANPIAYRVP MASAQVKSAV 190 200 210 220 230 240 LLAGLNTPGV TTVIEPVMTR DHTEKMLQGF GADLTVETDK DGVRHIRIVG QGKLTGQTID 250 260 270 280 290 300 VPGDPSSTAF PLVAALLVEG SDVTIRNVLM NPTRTGLILT LQEMGADIEI IDPRLAGGED 310 320 330 340 350 360 VADLRVKASK LKGVVVPPER APSMIDEYPV LAIAASFAEG ETVMDGLDEL RVKESDRLAA 370 380 390 400 410 420 VARGLEANGV DCTEGEMSLT VRGRPGGKGL GGGTVGTHLD HRIAMSFLVM GLASEKPVTV 430 440 450 DDSTMIATSF PEFMGMMAGL GAKIAESGAE

References

[1]

"The genome sequence of the facultative intracellular pathogen Brucella melitensis." DelVecchio V.G., Kapatral V., Redkar R.J., Patra G., Mujer C., Los T., Ivanova N., Anderson I., Bhattacharyya A., Lykidis A., Reznik G., Jablonski L., Larsen N., D'Souza M., Bernal A., Mazur M., Goltsman E., Selkov E. , Elzer P.H., Hagius S., O'Callaghan D., Letesson J.-J., Haselkorn R., Kyrpides N.C., Overbeek R. Proc. Natl. Acad. Sci. U.S.A. 99:443-448(2002) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: 16M / ATCC 23456 / NCTC 10094.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AE008917 Genomic DNA. Translation: AAL53098.1. Different initiation.
PIR	AG3491.
RefSeq	NP_540834.1. NC_003317.1.
3D structure databases
ProteinModelPortal	Q8YEG1.
SMR	Q8YEG1. Positions 8-447.
ModBase	Search...
Protein-protein interaction databases
STRING	224914.BMEI1917.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblBacteria	AAL53098; AAL53098; BMEI1917.
GeneID	1197628.
KEGG	bme:BMEI1917.
PATRIC	17854268. VBIBruMel92729_2119.
Organism-specific databases
CMR	Search...
Phylogenomic databases
HOGENOM	HOG000247371.
KO	K00800.
OMA	DPSAACF.
ProtClustDB	PRK02427.
Enzyme and pathway databases
UniPathway	UPA00053; UER00089.
Family and domain databases
Gene3D	3.65.10.10. 2 hits.
HAMAP	MF_00210. EPSP_synth.
InterPro	IPR001986. Enolpyruvate_Tfrase_dom. IPR006264. EPSP_synthase. IPR023193. EPSP_synthase_CS. IPR013792. RNA3'P_cycl/enolpyr_Trfase_a/b. [Graphical view]
Pfam	PF00275. EPSP_synthase. 1 hit. [Graphical view]
PIRSF	PIRSF000505. EPSPS. 1 hit.
SUPFAM	SSF55205. RNA3'_cycl/enolpyr_transf_A/B. 1 hit.
TIGRFAMs	TIGR01356. aroA. 1 hit.
PROSITE	PS00104. EPSP_SYNTHASE_1. 1 hit. PS00885. EPSP_SYNTHASE_2. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

AROA_BRUME

Accession

Primary (citable) accession number: Q8YEG1

Entry history

Integrated into UniProtKB/Swiss-Prot:	June 6, 2003
Last sequence update:	June 6, 2003
Last modified:	May 1, 2013
This is version 75 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program