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Q8YEB7

- PNP_BRUME

UniProt

Q8YEB7 - PNP_BRUME

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Protein
Polyribonucleotide nucleotidyltransferase
Gene
pnp, BMEI1961
Organism
Brucella melitensis biotype 1 (strain 16M / ATCC 23456 / NCTC 10094)
Status
Reviewed - Annotation score: 3 out of 5 - Protein inferred from homologyi

Functioni

Involved in mRNA degradation. Catalyzes the phosphorolysis of single-stranded polyribonucleotides processively in the 3'- to 5'-direction By similarity.UniRule annotation

Catalytic activityi

RNA(n+1) + phosphate = RNA(n) + a nucleoside diphosphate.UniRule annotation

Cofactori

Magnesium By similarity.UniRule annotation

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Metal bindingi488 – 4881Magnesium By similarity
Metal bindingi494 – 4941Magnesium By similarity

GO - Molecular functioni

  1. 3'-5'-exoribonuclease activity Source: InterPro
  2. RNA binding Source: UniProtKB-HAMAP
  3. magnesium ion binding Source: UniProtKB-HAMAP
  4. polyribonucleotide nucleotidyltransferase activity Source: UniProtKB-HAMAP

GO - Biological processi

  1. RNA processing Source: InterPro
  2. mRNA catabolic process Source: UniProtKB-HAMAP
Complete GO annotation...

Keywords - Molecular functioni

Nucleotidyltransferase, Transferase

Keywords - Ligandi

Magnesium, Metal-binding, RNA-binding

Names & Taxonomyi

Protein namesi
Recommended name:
Polyribonucleotide nucleotidyltransferase (EC:2.7.7.8)
Alternative name(s):
Polynucleotide phosphorylase
Short name:
PNPase
Gene namesi
Name:pnp
Ordered Locus Names:BMEI1961
OrganismiBrucella melitensis biotype 1 (strain 16M / ATCC 23456 / NCTC 10094)
Taxonomic identifieri224914 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaAlphaproteobacteriaRhizobialesBrucellaceaeBrucella
ProteomesiUP000000419: Chromosome I

Subcellular locationi

Cytoplasm By similarity UniRule annotation

GO - Cellular componenti

  1. cytoplasm Source: UniProtKB-SubCell
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 714714Polyribonucleotide nucleotidyltransferaseUniRule annotation
PRO_0000329547Add
BLAST

Interactioni

Protein-protein interaction databases

STRINGi224914.BMEI1961.

Structurei

3D structure databases

ProteinModelPortaliQ8YEB7.

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini555 – 61460KH
Add
BLAST
Domaini624 – 69269S1 motif
Add
BLAST

Sequence similaritiesi

Contains 1 KH domain.
Contains 1 S1 motif domain.

Phylogenomic databases

HOGENOMiHOG000218327.
KOiK00962.
OMAiEYIINPD.
OrthoDBiEOG6WT8CC.

Family and domain databases

Gene3Di1.10.10.400. 1 hit.
2.40.50.140. 1 hit.
3.30.1370.10. 1 hit.
3.30.230.70. 2 hits.
HAMAPiMF_01595. PNPase.
InterProiIPR001247. ExoRNase_PH_dom1.
IPR015847. ExoRNase_PH_dom2.
IPR004087. KH_dom.
IPR004088. KH_dom_type_1.
IPR012340. NA-bd_OB-fold.
IPR012162. PNPase.
IPR027408. PNPase/RNase_PH_dom.
IPR015848. PNPase_PH_RNA-bd_bac/org-type.
IPR003029. Rbsml_prot_S1_RNA-bd_dom.
IPR020568. Ribosomal_S5_D2-typ_fold.
IPR022967. RNA-binding_domain_S1.
[Graphical view]
PANTHERiPTHR11252. PTHR11252. 1 hit.
PfamiPF00013. KH_1. 1 hit.
PF03726. PNPase. 1 hit.
PF01138. RNase_PH. 2 hits.
PF03725. RNase_PH_C. 2 hits.
PF00575. S1. 1 hit.
[Graphical view]
PIRSFiPIRSF005499. PNPase. 1 hit.
SMARTiSM00322. KH. 1 hit.
SM00316. S1. 1 hit.
[Graphical view]
SUPFAMiSSF50249. SSF50249. 1 hit.
SSF54211. SSF54211. 2 hits.
SSF54791. SSF54791. 1 hit.
SSF55666. SSF55666. 2 hits.
TIGRFAMsiTIGR03591. polynuc_phos. 1 hit.
PROSITEiPS50084. KH_TYPE_1. 1 hit.
PS50126. S1. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q8YEB7-1 [UniParc]FASTAAdd to Basket

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MFNTHKVEIE WGGRPLTLET GKIARQADGA VLATYGETAV LATVVSAKEP    50
KPGQDFFPLT VNYQEKTYAA GKIPGGYFKR EGRPSENETL VSRLIDRPIR 100
PLFVDGYKND TQVVITVLQH DLENNPDILS MVAASAALTI SGVPFMGPIS 150
GARVGYIDGE YVLNPNIDEM PESKLDLVVA GTSEAVLMVE SEAQELPEDV 200
MLGAVMFGHK SFQPVIDAII KLAEVAAKEP RDFQPEDLSE LEAKVLAVVE 250
NDLREAYKIT EKQARYAAVD AAKAKAEEHF FPEGVEETEM SAEQFATIFK 300
HLQAKIVRWN ILDTGNRIDG RDLSTVRPIV SEVGILPRTH GSALFTRGET 350
QAIVVATLGT GEDEQMIDAL TGTYKESFML HYNFPPYSVG ETGRMGSPGR 400
REIGHGKLAW RAIHPMLPAA EQFPYTIRAV SEITESNGSS SMATVCGTSL 450
ALMDAGVPIV RPVAGIAMGL IKEGERFAVL SDILGDEDHL GDMDFKVAGT 500
EFGITSLQMD IKIDGITEEI MKVALEQAKG GRVHILGEMA KAISSSRAEL 550
GEFAPRIEVM NIPTDKIRDV IGSGGKVIRE IVEKTGAKIN IEDDGTVKIA 600
SSNGKEIEAA KKWIHSIVAE PEVGEIYEGT VVKTADFGAF VNFFGPRDGL 650
VHISQLAADR VAKTTDVVKE GQKVWVKLMG FDERGKVRLS MKVVDQETGK 700
EIVAEKKKEE VDAE 714
Length:714
Mass (Da):77,731
Last modified:March 1, 2002 - v1
Checksum:i40F2EC5F6649A7F6
GO

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AE008917 Genomic DNA. Translation: AAL53142.1.
PIRiAC3497.
RefSeqiNP_540878.1. NC_003317.1.

Genome annotation databases

EnsemblBacteriaiAAL53142; AAL53142; BMEI1961.
GeneIDi1197672.
KEGGibme:BMEI1961.
PATRICi17795934. VBIBruMel146950_1238.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AE008917 Genomic DNA. Translation: AAL53142.1 .
PIRi AC3497.
RefSeqi NP_540878.1. NC_003317.1.

3D structure databases

ProteinModelPortali Q8YEB7.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

STRINGi 224914.BMEI1961.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblBacteriai AAL53142 ; AAL53142 ; BMEI1961 .
GeneIDi 1197672.
KEGGi bme:BMEI1961.
PATRICi 17795934. VBIBruMel146950_1238.

Phylogenomic databases

HOGENOMi HOG000218327.
KOi K00962.
OMAi EYIINPD.
OrthoDBi EOG6WT8CC.

Family and domain databases

Gene3Di 1.10.10.400. 1 hit.
2.40.50.140. 1 hit.
3.30.1370.10. 1 hit.
3.30.230.70. 2 hits.
HAMAPi MF_01595. PNPase.
InterProi IPR001247. ExoRNase_PH_dom1.
IPR015847. ExoRNase_PH_dom2.
IPR004087. KH_dom.
IPR004088. KH_dom_type_1.
IPR012340. NA-bd_OB-fold.
IPR012162. PNPase.
IPR027408. PNPase/RNase_PH_dom.
IPR015848. PNPase_PH_RNA-bd_bac/org-type.
IPR003029. Rbsml_prot_S1_RNA-bd_dom.
IPR020568. Ribosomal_S5_D2-typ_fold.
IPR022967. RNA-binding_domain_S1.
[Graphical view ]
PANTHERi PTHR11252. PTHR11252. 1 hit.
Pfami PF00013. KH_1. 1 hit.
PF03726. PNPase. 1 hit.
PF01138. RNase_PH. 2 hits.
PF03725. RNase_PH_C. 2 hits.
PF00575. S1. 1 hit.
[Graphical view ]
PIRSFi PIRSF005499. PNPase. 1 hit.
SMARTi SM00322. KH. 1 hit.
SM00316. S1. 1 hit.
[Graphical view ]
SUPFAMi SSF50249. SSF50249. 1 hit.
SSF54211. SSF54211. 2 hits.
SSF54791. SSF54791. 1 hit.
SSF55666. SSF55666. 2 hits.
TIGRFAMsi TIGR03591. polynuc_phos. 1 hit.
PROSITEi PS50084. KH_TYPE_1. 1 hit.
PS50126. S1. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

  1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: 16M / ATCC 23456 / NCTC 10094.

Entry informationi

Entry nameiPNP_BRUME
AccessioniPrimary (citable) accession number: Q8YEB7
Entry historyi
Integrated into UniProtKB/Swiss-Prot: April 29, 2008
Last sequence update: March 1, 2002
Last modified: May 14, 2014
This is version 76 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome

Documents

  1. Brucella melitensis
    Brucella melitensis (strain 16M): entries and gene names
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

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