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Q8YEA9

- TRMB_BRUME

UniProt

Q8YEA9 - TRMB_BRUME

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Protein

tRNA (guanine-N(7)-)-methyltransferase

Gene

trmB

Organism
Brucella melitensis biotype 1 (strain 16M / ATCC 23456 / NCTC 10094)
Status
Reviewed - Annotation score: 2 out of 5- Protein inferred from homologyi

Functioni

Catalyzes the formation of N(7)-methylguanine at position 46 (m7G46) in tRNA.UniRule annotation

Catalytic activityi

S-adenosyl-L-methionine + guanine(46) in tRNA = S-adenosyl-L-homocysteine + N(7)-methylguanine(46) in tRNA.UniRule annotation

Pathwayi

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei64 – 641S-adenosyl-L-methionineUniRule annotation
Binding sitei89 – 891S-adenosyl-L-methionineUniRule annotation
Binding sitei116 – 1161S-adenosyl-L-methionineUniRule annotation
Active sitei138 – 1381By similarity
Binding sitei138 – 1381S-adenosyl-L-methionineUniRule annotation
Binding sitei142 – 1421SubstrateUniRule annotation
Binding sitei174 – 1741SubstrateUniRule annotation

GO - Molecular functioni

  1. tRNA (guanine-N7-)-methyltransferase activity Source: UniProtKB-HAMAP
Complete GO annotation...

Keywords - Molecular functioni

Methyltransferase, Transferase

Keywords - Biological processi

tRNA processing

Keywords - Ligandi

S-adenosyl-L-methionine

Enzyme and pathway databases

UniPathwayiUPA00989.

Names & Taxonomyi

Protein namesi
Recommended name:
tRNA (guanine-N(7)-)-methyltransferaseUniRule annotation (EC:2.1.1.33UniRule annotation)
Alternative name(s):
tRNA (guanine(46)-N(7))-methyltransferaseUniRule annotation
tRNA(m7G46)-methyltransferaseUniRule annotation
Gene namesi
Name:trmBUniRule annotation
Ordered Locus Names:BMEI1969
OrganismiBrucella melitensis biotype 1 (strain 16M / ATCC 23456 / NCTC 10094)
Taxonomic identifieri224914 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaAlphaproteobacteriaRhizobialesBrucellaceaeBrucella
ProteomesiUP000000419: Chromosome I

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 233233tRNA (guanine-N(7)-)-methyltransferasePRO_0000171305Add
BLAST

Interactioni

Protein-protein interaction databases

STRINGi224914.BMEI1969.

Structurei

3D structure databases

ProteinModelPortaliQ8YEA9.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni212 – 2154Substrate bindingUniRule annotation

Sequence similaritiesi

Belongs to the class I-like SAM-binding methyltransferase superfamily. TrmB family.UniRule annotation

Phylogenomic databases

HOGENOMiHOG000073969.
KOiK03439.
OMAiRAFYGRR.
OrthoDBiEOG6K6VBC.

Family and domain databases

Gene3Di3.40.50.150. 1 hit.
HAMAPiMF_01057. tRNA_methyltr_TrmB.
InterProiIPR029063. SAM-dependent_MTases-like.
IPR003358. tRNA_(Gua-N-7)_MeTrfase.
[Graphical view]
PfamiPF02390. Methyltransf_4. 1 hit.
[Graphical view]
SUPFAMiSSF53335. SSF53335. 1 hit.
PROSITEiPS51625. SAM_MT_TRMB. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q8YEA9-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MIDENHPMRA AGNFFGRRHG KPLRPHQSNL FEDLLPRLKL DLATPAPQDL
60 70 80 90 100
RSLFEAPVET VRMEIGFGGG EHLHHESGRY PQSGFIGVEP FINGMAKMLA
110 120 130 140 150
ALDQAPRPNL RLYDEDATAV LDWLPDASLA GIDLFYPDPW HKRRHWKRRF
160 170 180 190 200
VSDANLDRFA RVLKPGAKFR FASDIEHYVN WTLQHCRRHA AFDWQAESPA
210 220 230
DWNDAYEGWP GTRYEAKAFR EGRRAAYLTF IRR
Length:233
Mass (Da):27,006
Last modified:March 1, 2002 - v1
Checksum:i9CB9E041176BAFCF
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AE008917 Genomic DNA. Translation: AAL53150.1.
PIRiAC3498.
RefSeqiNP_540886.1. NC_003317.1.

Genome annotation databases

EnsemblBacteriaiAAL53150; AAL53150; BMEI1969.
GeneIDi1197680.
KEGGibme:BMEI1969.
PATRICi17795916. VBIBruMel146950_1229.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AE008917 Genomic DNA. Translation: AAL53150.1 .
PIRi AC3498.
RefSeqi NP_540886.1. NC_003317.1.

3D structure databases

ProteinModelPortali Q8YEA9.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

STRINGi 224914.BMEI1969.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblBacteriai AAL53150 ; AAL53150 ; BMEI1969 .
GeneIDi 1197680.
KEGGi bme:BMEI1969.
PATRICi 17795916. VBIBruMel146950_1229.

Phylogenomic databases

HOGENOMi HOG000073969.
KOi K03439.
OMAi RAFYGRR.
OrthoDBi EOG6K6VBC.

Enzyme and pathway databases

UniPathwayi UPA00989 .

Family and domain databases

Gene3Di 3.40.50.150. 1 hit.
HAMAPi MF_01057. tRNA_methyltr_TrmB.
InterProi IPR029063. SAM-dependent_MTases-like.
IPR003358. tRNA_(Gua-N-7)_MeTrfase.
[Graphical view ]
Pfami PF02390. Methyltransf_4. 1 hit.
[Graphical view ]
SUPFAMi SSF53335. SSF53335. 1 hit.
PROSITEi PS51625. SAM_MT_TRMB. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

  1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: 16M / ATCC 23456 / NCTC 10094.

Entry informationi

Entry nameiTRMB_BRUME
AccessioniPrimary (citable) accession number: Q8YEA9
Entry historyi
Integrated into UniProtKB/Swiss-Prot: June 16, 2003
Last sequence update: March 1, 2002
Last modified: October 1, 2014
This is version 71 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome

Documents

  1. Brucella melitensis
    Brucella melitensis (strain 16M): entries and gene names
  2. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  3. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3