Reviewed, UniProtKB/Swiss-Prot Q8YE73 (SYFA_BRUME)

Names and origin

Protein names

Recommended name:
    Phenylalanyl-tRNA synthetase alpha chain
    EC=6.1.1.20
Alternative name(s):
    Phenylalanine--tRNA ligase alpha chain
      Short name=PheRS

Gene names

Name:	pheS
Ordered Locus Names:	BMEI2005

Organism

Brucella melitensis [Complete proteome] [HAMAP]

Taxonomic identifier

29459 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Alphaproteobacteria › Rhizobiales › Brucellaceae › Brucella

Protein attributes

Sequence length	369 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Inferred from homology.

General annotation (Comments)

Catalytic activity	ATP + L-phenylalanine + tRNA(Phe) = AMP + diphosphate + L-phenylalanyl-tRNA(Phe). HAMAP MF_00281
Cofactor	Binds 2 magnesium ions per tetramer By similarity.
Subunit structure	Tetramer of two alpha and two beta chains By similarity.
Subcellular location	Cytoplasm. HAMAP MF_00281
Sequence similarities	Belongs to the class-II aminoacyl-tRNA synthetase family. Phe-tRNA synthetase alpha chain type 1 subfamily.

Ontologies

Keywords
Biological process	Protein biosynthesis
Cellular component	Cytoplasm
Ligand	ATP-binding Magnesium Metal-binding Nucleotide-binding
Molecular function	Aminoacyl-tRNA synthetase Ligase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	phenylalanyl-tRNA aminoacylation Inferred from electronic annotation. Source: HAMAP
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	ATP binding Inferred from electronic annotation. Source: HAMAP magnesium ion binding Inferred from electronic annotation. Source: HAMAP phenylalanine-tRNA ligase activity Inferred from electronic annotation. Source: HAMAP
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 369	369	Phenylalanyl-tRNA synthetase alpha chain HAMAP MF_00281		PRO_0000126674
Sites
	Metal binding	269	1	Magnesium By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q8YE73-1 [UniParc]. Last modified March 1, 2002. Version 1. Checksum: BC2B81DE7F180926	FASTA	369	41,540
	10 20 30 40 50 60 MSDLEQLERQ ILEDIAAAVD EQGIEAVRVA ALGKKGTVSE KLKTLGGMSP EERQMQGPAI 70 80 90 100 110 120 NGLKNRVTEA LSERRTELRK AAVAARLERE KVDVTLPVRE SAASRGRIHP ISQVIDEITA 130 140 150 160 170 180 IFADMGFSIA EGPDIETDYY NFTALNFPEG HPAREMHDTF FFNPDEKGER KLLRTHTSPV 190 200 210 220 230 240 QVHTMEKFAA MRDKEGRDEP IRIVIPGKTY RMDSDATHSP MFHQVEGLVV DKSANVANMK 250 260 270 280 290 300 WVLEEFCKAF FEVPSVKMRM RPSFFPFTEP SVEVDIQCDR SGPHVKFGEG NDWLEILGCG 310 320 330 340 350 360 MVHPNVLRMS GYDPEVYQGF AWGMGIGRIA MLKYGMPDLR AFFDADVRWI EHYGFRPLDI PTLFGGLSA

References

[1]

"The genome sequence of the facultative intracellular pathogen Brucella melitensis." DelVecchio V.G., Kapatral V., Redkar R.J., Patra G., Mujer C., Los T., Ivanova N., Anderson I., Bhattacharyya A., Lykidis A., Reznik G., Jablonski L., Larsen N., D'Souza M., Bernal A., Mazur M., Goltsman E., Selkov E. , Elzer P.H., Hagius S., O'Callaghan D., Letesson J.-J., Haselkorn R., Kyrpides N.C., Overbeek R. Proc. Natl. Acad. Sci. U.S.A. 99:443-448(2002) [PubMed: 11756688] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: 16M / ATCC 23456 / NCTC 10094 / Biotype 1.

Cross-references

Sequence databases
EMBLGenBankDDBJ	AE008917 Genomic DNA. Translation: AAL53186.1.
PIR	AG3502.
RefSeq	NP_540922.1.
3D structure databases
HSSP	HSSP built from PDB template 1JJC based on UniProtKB P27001.
ModBase	Search...
Genome annotation databases
GeneID	1197716.
GenomeReviews	Gene locus BMEI2005 in contig AE008917_GR.
KEGG	bme:BMEI2005.
NMPDR	fig|224914.1.peg.2004.
Organism-specific databases
CMR	Search...
Phylogenomic databases
HOGENOM	Q8YE73.
OMA	FRASYFP.
Enzyme and pathway databases
BioCyc	BMEL224914:BMEI2005-MON.
BRENDA	6.1.1.20. 277959.
Family and domain databases
HAMAP	MF_00281. [Tree]
InterPro	IPR006195. aa-tRNA-synth_II_cons-reg. IPR004529. Phe-tRNA-synth_IIc_asu. IPR004188. Phe-tRNA_synth_II_N. IPR002319. Phenylalanyl-tRNA_Synthase_acu. [Graphical view]
PANTHER	PTHR11538. tRNA-synt_2d. 1 hit.
Pfam	PF02912. Phe_tRNA-synt_N. 1 hit. PF01409. tRNA-synt_2d. 1 hit. [Graphical view]
TIGRFAMs	TIGR00468. pheS. 1 hit.
PROSITE	PS50862. AA_TRNA_LIGASE_II. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

SYFA_BRUME

Accession

Primary (citable) accession number: Q8YE73

Entry history

Integrated into UniProtKB/Swiss-Prot:	October 19, 2002
Last sequence update:	March 1, 2002
Last modified:	November 3, 2009
This is version 50 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)