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Protein

Shikimate dehydrogenase (NADP(+))

Gene

aroE

Organism
Brucella melitensis biotype 1 (strain 16M / ATCC 23456 / NCTC 10094)
Status
Reviewed-Annotation score: -Protein inferred from homologyi

Functioni

Involved in the biosynthesis of the chorismate, which leads to the biosynthesis of aromatic amino acids. Catalyzes the reversible NADPH linked reduction of 3-dehydroshikimate (DHSA) to yield shikimate (SA).UniRule annotation

Catalytic activityi

Shikimate + NADP+ = 3-dehydroshikimate + NADPH.UniRule annotation

Pathwayi: chorismate biosynthesis

This protein is involved in step 4 of the subpathway that synthesizes chorismate from D-erythrose 4-phosphate and phosphoenolpyruvate.UniRule annotation
Proteins known to be involved in the 7 steps of the subpathway in this organism are:
  1. no protein annotated in this organism
  2. 3-dehydroquinate synthase (aroB)
  3. 3-dehydroquinate dehydratase (aroQ)
  4. Shikimate dehydrogenase (NADP(+)) (aroE)
  5. Shikimate kinase (aroK)
  6. 3-phosphoshikimate 1-carboxyvinyltransferase (aroA)
  7. Chorismate synthase (aroC)
This subpathway is part of the pathway chorismate biosynthesis, which is itself part of Metabolic intermediate biosynthesis.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes chorismate from D-erythrose 4-phosphate and phosphoenolpyruvate, the pathway chorismate biosynthesis and in Metabolic intermediate biosynthesis.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei69ShikimateUniRule annotation1
Active sitei73Proton acceptorUniRule annotation1
Binding sitei85NADPUniRule annotation1
Binding sitei94ShikimateUniRule annotation1
Binding sitei109ShikimateUniRule annotation1
Binding sitei226NADP; via carbonyl oxygenUniRule annotation1
Binding sitei228ShikimateUniRule annotation1
Binding sitei249NADP; via carbonyl oxygenUniRule annotation1

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi134 – 138NADPUniRule annotation5
Nucleotide bindingi158 – 163NADPUniRule annotation6

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionOxidoreductase
Biological processAmino-acid biosynthesis, Aromatic amino acid biosynthesis
LigandNADP

Enzyme and pathway databases

UniPathwayiUPA00053; UER00087

Names & Taxonomyi

Protein namesi
Recommended name:
Shikimate dehydrogenase (NADP(+))UniRule annotation (EC:1.1.1.25UniRule annotation)
Short name:
SDHUniRule annotation
Gene namesi
Name:aroEUniRule annotation
Ordered Locus Names:BMEI2058
OrganismiBrucella melitensis biotype 1 (strain 16M / ATCC 23456 / NCTC 10094)
Taxonomic identifieri224914 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaAlphaproteobacteriaRhizobialesBrucellaceaeBrucella
Proteomesi
  • UP000000419 Componenti: Chromosome I

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001359931 – 289Shikimate dehydrogenase (NADP(+))Add BLAST289

Interactioni

Subunit structurei

Homodimer.UniRule annotation

Protein-protein interaction databases

STRINGi224914.BAWG_0190

Structurei

3D structure databases

ProteinModelPortaliQ8YE20
SMRiQ8YE20
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni22 – 24Shikimate bindingUniRule annotation3

Sequence similaritiesi

Belongs to the shikimate dehydrogenase family.UniRule annotation

Phylogenomic databases

eggNOGiENOG4105E2X Bacteria
COG0169 LUCA
HOGENOMiHOG000237876
KOiK00014
OMAiTHPCKQL

Family and domain databases

HAMAPiMF_00222 Shikimate_DH_AroE, 1 hit
InterProiView protein in InterPro
IPR036291 NAD(P)-bd_dom_sf
IPR011342 Shikimate_DH
IPR013708 Shikimate_DH-bd_N
IPR022893 Shikimate_DH_fam
IPR006151 Shikm_DH/Glu-tRNA_Rdtase
PfamiView protein in Pfam
PF01488 Shikimate_DH, 1 hit
PF08501 Shikimate_dh_N, 1 hit
SUPFAMiSSF51735 SSF51735, 1 hit
TIGRFAMsiTIGR00507 aroE, 1 hit

Sequencei

Sequence statusi: Complete.

Q8YE20-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MDDKSMARGR KAFVTGFPIR HSRSPLIHGF WLKELGIDGS YEAVEVKPED
60 70 80 90 100
FSSFAASLAA NGFAGGNVTI PHKEAAYAAA ESLDEAARAI GAVNTLWLEN
110 120 130 140 150
GRLCGGNTDA YGFAANLDAS APGWDKADRA LVLGAGGASR AVVHALLSRG
160 170 180 190 200
VCHVSVVNRT LSRAEELAAH FGARVYAHGW DEAQALVSNA GLIVNTTALG
210 220 230 240 250
MSGHGEGQDF PIDLTCAPKE AVATDIVYVP LRTAFLNKAE KAGLKTVDGL
260 270 280
GMLLHQAVPG FERWFGQRPQ VTQALREHIL ADMAKAGAL
Length:289
Mass (Da):30,490
Last modified:March 1, 2002 - v1
Checksum:iAC07A13B436754D7
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AE008917 Genomic DNA Translation: AAL53239.1
PIRiAD3509
RefSeqiWP_002965133.1, NZ_GG703778.1

Genome annotation databases

EnsemblBacteriaiAAL53239; AAL53239; BMEI2058
GeneIDi29594947
KEGGibme:BMEI2058

Similar proteinsi

Entry informationi

Entry nameiAROE_BRUME
AccessioniPrimary (citable) accession number: Q8YE20
Entry historyiIntegrated into UniProtKB/Swiss-Prot: October 19, 2002
Last sequence update: March 1, 2002
Last modified: March 28, 2018
This is version 101 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome

Documents

  1. Brucella melitensis
    Brucella melitensis (strain 16M): entries and gene names
  2. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  3. SIMILARITY comments
    Index of protein domains and families

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