Q8YE20 (AROE_BRUME) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Shikimate dehydrogenase
EC=1.1.1.25

Gene names

Name:	aroE
Ordered Locus Names:	BMEI2058

Organism

Brucella melitensis biotype 1 (strain 16M / ATCC 23456 / NCTC 10094) [Complete proteome] [HAMAP]

Taxonomic identifier

224914 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Alphaproteobacteria › Rhizobiales › Brucellaceae › Brucella

Protein attributes

Sequence length	289 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Catalytic activity	Shikimate + NADP+ = 3-dehydroshikimate + NADPH. HAMAP MF_00222
Pathway	Metabolic intermediate biosynthesis; chorismate biosynthesis; chorismate from D-erythrose 4-phosphate and phosphoenolpyruvate: step 4/7. HAMAP MF_00222
Sequence similarities	Belongs to the shikimate dehydrogenase family.

Ontologies

Keywords
Biological process	Amino-acid biosynthesis Aromatic amino acid biosynthesis
Ligand	NADP
Molecular function	Oxidoreductase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	aromatic amino acid family biosynthetic process Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	NADP binding Inferred from electronic annotation. Source: InterPro shikimate 3-dehydrogenase (NADP+) activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 289	289	Shikimate dehydrogenase HAMAP MF_00222		PRO_0000135993
Regions
	Nucleotide binding	134 – 138	5	NADP By similarity
Sites
	Active site	73	1	Proton acceptor Potential

Sequences

	Sequence		Length	Mass (Da)
	Q8YE20 [UniParc]. Last modified March 1, 2002. Version 1. Checksum: AC07A13B436754D7	FASTA	289	30,490
	10 20 30 40 50 60 MDDKSMARGR KAFVTGFPIR HSRSPLIHGF WLKELGIDGS YEAVEVKPED FSSFAASLAA 70 80 90 100 110 120 NGFAGGNVTI PHKEAAYAAA ESLDEAARAI GAVNTLWLEN GRLCGGNTDA YGFAANLDAS 130 140 150 160 170 180 APGWDKADRA LVLGAGGASR AVVHALLSRG VCHVSVVNRT LSRAEELAAH FGARVYAHGW 190 200 210 220 230 240 DEAQALVSNA GLIVNTTALG MSGHGEGQDF PIDLTCAPKE AVATDIVYVP LRTAFLNKAE 250 260 270 280 KAGLKTVDGL GMLLHQAVPG FERWFGQRPQ VTQALREHIL ADMAKAGAL

References

[1]

"The genome sequence of the facultative intracellular pathogen Brucella melitensis." DelVecchio V.G., Kapatral V., Redkar R.J., Patra G., Mujer C., Los T., Ivanova N., Anderson I., Bhattacharyya A., Lykidis A., Reznik G., Jablonski L., Larsen N., D'Souza M., Bernal A., Mazur M., Goltsman E., Selkov E. , Elzer P.H., Hagius S., O'Callaghan D., Letesson J.-J., Haselkorn R., Kyrpides N.C., Overbeek R. Proc. Natl. Acad. Sci. U.S.A. 99:443-448(2002) [PubMed: 11756688] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: 16M / ATCC 23456 / NCTC 10094.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AE008917 Genomic DNA. Translation: AAL53239.1.
PIR	AD3509.
RefSeq	NP_540975.1. NC_003317.1.
3D structure databases
ProteinModelPortal	Q8YE20.
ModBase	Search...
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	1197769.
GenomeReviews	Gene locus BMEI2058 in contig AE008917_GR.
KEGG	bme:BMEI2058.
NMPDR	fig|224914.1.peg.2057.
PATRIC	17795715. VBIBruMel146950_1129.
Organism-specific databases
CMR	Search...
Phylogenomic databases
HOGENOM	HBG553408.
OMA	LHLKTHG.
PhylomeDB	Q8YE20.
ProtClustDB	PRK00258.
Enzyme and pathway databases
BioCyc	BMEL224914:BMEI2058-MONOMER.
Family and domain databases
HAMAP	MF_00222. Shikimate_DH_AroE. [Tree]
InterPro	IPR016040. NAD(P)-bd_dom. IPR011342. Shikimate_DH. IPR013708. Shikimate_DH-bd_N. IPR022893. Shikimate_quinate_DH. IPR006151. Shikm_DH/Glu-tRNA_Rdtase. [Graphical view]
Gene3D	G3DSA:3.40.50.720. NAD(P)-bd. 1 hit.
KO	K00014.
Pfam	PF01488. Shikimate_DH. 1 hit. PF08501. Shikimate_dh_N. 1 hit. [Graphical view]
TIGRFAMs	TIGR00507. AroE. 1 hit.
ProtoNet	Search...

Entry information

Entry name

AROE_BRUME

Accession

Primary (citable) accession number: Q8YE20

Entry history

Integrated into UniProtKB/Swiss-Prot:	October 19, 2002
Last sequence update:	March 1, 2002
Last modified:	January 25, 2012
This is version 70 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program