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Protein

Flagellin

Gene

fliC

Organism
Brucella melitensis biotype 1 (strain 16M / ATCC 23456 / NCTC 10094)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Experimental evidence at protein leveli

Functioni

Flagellin is the subunit protein which polymerizes to form the filaments of bacterial flagella (By similarity). The flagellum is required to cause a persistent disease in a murine model of infection.By similarity1 Publication

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Names & Taxonomyi

Protein namesi
Recommended name:
Flagellin
Gene namesi
Name:fliC
Ordered Locus Names:BMEII0150
OrganismiBrucella melitensis biotype 1 (strain 16M / ATCC 23456 / NCTC 10094)
Taxonomic identifieri224914 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaAlphaproteobacteriaRhizobialesBrucellaceaeBrucella
ProteomesiUP000000419 Componenti: Chromosome II

Subcellular locationi

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Bacterial flagellum, Secreted

Pathology & Biotechi

Disruption phenotypei

A fliC mutant is attenuated in a murine model of infection. As the infection progresses, the number of cfu per spleen from mice infected with the mutant is significantly lower than the number from mice infected with the wild-type.1 Publication

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 282282FlagellinPRO_0000323998Add
BLAST

Expressioni

Inductioni

Expressed at the onset of the exponential phase (after 4, 8 and 12 hours of culture) but not at later time points.

Interactioni

Protein-protein interaction databases

STRINGi224914.BAWG_1326.

Structurei

3D structure databases

ProteinModelPortaliQ8YDM5.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the bacterial flagellin family.Curated

Phylogenomic databases

HOGENOMiHOG000255145.
KOiK02406.
OMAiAMNSSID.
OrthoDBiEOG622PP4.

Family and domain databases

InterProiIPR001492. Flagellin.
IPR001029. Flagellin_D0/D1.
[Graphical view]
PfamiPF00700. Flagellin_C. 1 hit.
PF00669. Flagellin_N. 1 hit.
[Graphical view]
PRINTSiPR00207. FLAGELLIN.

Sequencei

Sequence statusi: Complete.

Q8YDM5-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MASILTNSSA LTALQTLAST NKSLESTQNR ISTGLRISEA SDNASYWSIA
60 70 80 90 100
TSMKSDNKAN SAVQDALGLG AGKVDTAYSA INKIRESVDD IKTKLVSAMG
110 120 130 140 150
ASTEDKGKIE TEIKSIVANI NSALSNANYA GSNLLNGPTT DLNVVASYNR
160 170 180 190 200
SGNAVAVDKI TVKATDTDAK TMVKDIVDAG FFTSASDDTA IGTALNTVET
210 220 230 240 250
ALASLATGAA TLGAAKSQID SQKSFLSGLQ DSIEKGVGTL VDADMNKESA
260 270 280
RLSALQVQQQ LGVQALSIAN SSNQSILSLF RG
Length:282
Mass (Da):29,069
Last modified:March 1, 2002 - v1
Checksum:iDF645A110EC1D4B0
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AE008918 Genomic DNA. Translation: AAL53391.1.
PIRiAD3528.
RefSeqiWP_004681009.1. NZ_GG703779.1.

Genome annotation databases

EnsemblBacteriaiAAL53391; AAL53391; BMEII0150.
KEGGibme:BMEII0150.
PATRICi17798345. VBIBruMel146950_1519.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AE008918 Genomic DNA. Translation: AAL53391.1.
PIRiAD3528.
RefSeqiWP_004681009.1. NZ_GG703779.1.

3D structure databases

ProteinModelPortaliQ8YDM5.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi224914.BAWG_1326.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiAAL53391; AAL53391; BMEII0150.
KEGGibme:BMEII0150.
PATRICi17798345. VBIBruMel146950_1519.

Phylogenomic databases

HOGENOMiHOG000255145.
KOiK02406.
OMAiAMNSSID.
OrthoDBiEOG622PP4.

Miscellaneous databases

PROiQ8YDM5.

Family and domain databases

InterProiIPR001492. Flagellin.
IPR001029. Flagellin_D0/D1.
[Graphical view]
PfamiPF00700. Flagellin_C. 1 hit.
PF00669. Flagellin_N. 1 hit.
[Graphical view]
PRINTSiPR00207. FLAGELLIN.
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: 16M / ATCC 23456 / NCTC 10094.
  2. "The sheathed flagellum of Brucella melitensis is involved in persistence in a murine model of infection."
    Fretin D., Fauconnier A., Koehler S., Halling S., Leonard S., Nijskens C., Ferooz J., Lestrate P., Delrue R.-M., Danese I., Vandenhaute J., Tibor A., DeBolle X., Letesson J.-J.
    Cell. Microbiol. 7:687-698(2005) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION IN INFECTION PERSISTENCE, EXPRESSION, DISRUPTION PHENOTYPE.
    Strain: 16M / ATCC 23456 / NCTC 10094.

Entry informationi

Entry nameiFLIC_BRUME
AccessioniPrimary (citable) accession number: Q8YDM5
Entry historyi
Integrated into UniProtKB/Swiss-Prot: March 18, 2008
Last sequence update: March 1, 2002
Last modified: July 22, 2015
This is version 76 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Caution

Despite the presence of a stop codon in position 243 in the fliF gene and in position 127 in the flhA gene, it has been shown that B.melitensis is able to express genes corresponding to the M ring, the hook and the filament of the flagellar apparatus in the early log phase of growth in 2YT broth. Under these conditions, a polar and sheathed flagellar structure is visible by transmission electron microscopy.Curated

Keywords - Technical termi

Complete proteome

Documents

  1. Brucella melitensis
    Brucella melitensis (strain 16M): entries and gene names
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.