Q8YDM5 (FLIC_BRUME) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Flagellin

Gene names

Name:	fliC
Ordered Locus Names:	BMEII0150

Organism

Brucella melitensis biotype 1 (strain 16M / ATCC 23456 / NCTC 10094) [Complete proteome] [HAMAP]

Taxonomic identifier

224914 [NCBI]

Taxonomic lineage

cellular organisms › Bacteria › Proteobacteria › Alphaproteobacteria › Rhizobiales › Brucellaceae › Brucella › Brucella melitensis › Brucella melitensis bv. 1

Protein attributes

Sequence length	282 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Flagellin is the subunit protein which polymerizes to form the filaments of bacterial flagella By similarity. The flagellum is required to cause a persistent disease in a murine model of infection. Ref.2
Subcellular location	Secreted. Bacterial flagellum.
Induction	Expressed at the onset of the exponential phase (after 4, 8 and 12 hours of culture) but not at later time points.
Disruption phenotype	A fliC mutant is attenuated in a murine model of infection. As the infection progresses, the number of cfu per spleen from mice infected with the mutant is significantly lower than the number from mice infected with the wild-type. Ref.2
Sequence similarities	Belongs to the bacterial flagellin family.
Caution	Despite the presence of a stop codon in position 243 in the fliF gene and in position 127 in the flhA gene, it has been shown that B.melitensis is able to express genes corresponding to the M ring, the hook and the filament of the flagellar apparatus in the early log phase of growth in 2YT broth. Under these conditions, a polar and sheathed flagellar structure is visible by transmission electron microscopy.

Ontologies

Keywords
Cellular component	Bacterial flagellum Secreted
Technical term	Complete proteome
Gene Ontology (GO)
Biological_process	ciliary or flagellar motility Inferred from electronic annotation. Source: InterPro
Cellular_component	bacterial-type flagellum filament Inferred from electronic annotation. Source: InterPro extracellular region Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular_function	structural molecule activity Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 282	282	Flagellin		PRO_0000323998

Sequences

	Sequence		Length	Mass (Da)
	Q8YDM5 [UniParc]. Last modified March 1, 2002. Version 1. Checksum: DF645A110EC1D4B0	FASTA	282	29,069
	10 20 30 40 50 60 MASILTNSSA LTALQTLAST NKSLESTQNR ISTGLRISEA SDNASYWSIA TSMKSDNKAN 70 80 90 100 110 120 SAVQDALGLG AGKVDTAYSA INKIRESVDD IKTKLVSAMG ASTEDKGKIE TEIKSIVANI 130 140 150 160 170 180 NSALSNANYA GSNLLNGPTT DLNVVASYNR SGNAVAVDKI TVKATDTDAK TMVKDIVDAG 190 200 210 220 230 240 FFTSASDDTA IGTALNTVET ALASLATGAA TLGAAKSQID SQKSFLSGLQ DSIEKGVGTL 250 260 270 280 VDADMNKESA RLSALQVQQQ LGVQALSIAN SSNQSILSLF RG

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"The genome sequence of the facultative intracellular pathogen Brucella melitensis." DelVecchio V.G., Kapatral V., Redkar R.J., Patra G., Mujer C., Los T., Ivanova N., Anderson I., Bhattacharyya A., Lykidis A., Reznik G., Jablonski L., Larsen N., D'Souza M., Bernal A., Mazur M., Goltsman E., Selkov E. , Elzer P.H., Hagius S., O'Callaghan D., Letesson J.-J., Haselkorn R., Kyrpides N.C., Overbeek R. Proc. Natl. Acad. Sci. U.S.A. 99:443-448(2002) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: 16M / ATCC 23456 / NCTC 10094.
[2]	"The sheathed flagellum of Brucella melitensis is involved in persistence in a murine model of infection." Fretin D., Fauconnier A., Koehler S., Halling S., Leonard S., Nijskens C., Ferooz J., Lestrate P., Delrue R.-M., Danese I., Vandenhaute J., Tibor A., DeBolle X., Letesson J.-J. Cell. Microbiol. 7:687-698(2005) [PubMed] [Europe PMC] [Abstract] Cited for: FUNCTION IN INFECTION PERSISTENCE, EXPRESSION, DISRUPTION PHENOTYPE. Strain: 16M / ATCC 23456 / NCTC 10094.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AE008918 Genomic DNA. Translation: AAL53391.1.
PIR	AD3528.
RefSeq	NP_541127.1. NC_003318.1.
3D structure databases
ProteinModelPortal	Q8YDM5.
ModBase	Search...
Protein-protein interaction databases
STRING	224914.BMEII0150.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblBacteria	AAL53391; AAL53391; BMEII0150.
GeneID	1197921.
KEGG	bme:BMEII0150.
PATRIC	17798345. VBIBruMel146950_1519.
Organism-specific databases
CMR	Search...
Phylogenomic databases
HOGENOM	HOG000255145.
KO	K02406.
OMA	ININVEG.
ProtClustDB	CLSK898717.
Family and domain databases
InterPro	IPR001492. Flagellin. IPR001029. Flagellin_D0/D1. [Graphical view]
Pfam	PF00700. Flagellin_C. 1 hit. PF00669. Flagellin_N. 1 hit. [Graphical view]
PRINTS	PR00207. FLAGELLIN.
ProtoNet	Search...

Entry information

Entry name

FLIC_BRUME

Accession

Primary (citable) accession number: Q8YDM5

Entry history

Integrated into UniProtKB/Swiss-Prot:	March 18, 2008
Last sequence update:	March 1, 2002
Last modified:	May 1, 2013
This is version 63 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program