Q8YDA5 (ARGB_BRUME) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Acetylglutamate kinase
EC=2.7.2.8

Alternative name(s):
N-acetyl-L-glutamate 5-phosphotransferase
NAG kinase
Short name=AGK

Gene names

Name:	argB
Ordered Locus Names:	BMEII0273

Organism

Brucella melitensis biotype 1 (strain 16M / ATCC 23456 / NCTC 10094) [Complete proteome] [HAMAP]

Taxonomic identifier

224914 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Alphaproteobacteria › Rhizobiales › Brucellaceae › Brucella

Protein attributes

Sequence length	296 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Catalytic activity	ATP + N-acetyl-L-glutamate = ADP + N-acetyl-L-glutamate 5-phosphate. HAMAP MF_00082_B
Pathway	Amino-acid biosynthesis; L-arginine biosynthesis; N(2)-acetyl-L-ornithine from L-glutamate: step 2/4. HAMAP MF_00082_B
Subcellular location	Cytoplasm By similarity HAMAP MF_00082_B.
Sequence similarities	Belongs to the acetylglutamate kinase family.
Sequence caution	The sequence AAL53514.1 differs from that shown. Reason: Erroneous initiation.

Ontologies

Keywords
Biological process	Amino-acid biosynthesis Arginine biosynthesis
Cellular component	Cytoplasm
Ligand	ATP-binding Nucleotide-binding
Molecular function	Kinase Transferase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	arginine biosynthetic process Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW acetylglutamate kinase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 296	296	Acetylglutamate kinase HAMAP MF_00082_B		PRO_0000112596
Regions
	Region	67 – 68	2	Substrate binding By similarity
Sites
	Binding site	89	1	Substrate By similarity
	Binding site	194	1	Substrate By similarity
	Site	32	1	Transition state stabilizer By similarity
	Site	254	1	Transition state stabilizer By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q8YDA5 [UniParc]. Last modified November 7, 2003. Version 3. Checksum: 5875CCF2D28AE18E	FASTA	296	31,498
	10 20 30 40 50 60 MTTLENPEMQ AQLLSAALPY MQRYENKHVV VKYGGHAMGN PELGKAFARD VALLKQSGVN 70 80 90 100 110 120 PIVVHGGGPQ IQAMLTKLGI ESRFEGGLRV TDEKTVEVVE MVLAGSINKE IVALINAEGE 130 140 150 160 170 180 WAIGLCGKDG NMVFAQKAHK TVIDPDSNIE KVLDLGFVGE PAEVDRTLLD LLARSEMIPV 190 200 210 220 230 240 IAPVAPGRDG HTYNINADTF AGAIAGALAA TRLLFLTNVP GVLDKDKKLI KELSVADAQA 250 260 270 280 290 LIRDGTISGG MIPKVETCID AIRRGVEGVV ILNGKTPHSV LLELFTEHGA GTLIVP

References

[1]

"The genome sequence of the facultative intracellular pathogen Brucella melitensis." DelVecchio V.G., Kapatral V., Redkar R.J., Patra G., Mujer C., Los T., Ivanova N., Anderson I., Bhattacharyya A., Lykidis A., Reznik G., Jablonski L., Larsen N., D'Souza M., Bernal A., Mazur M., Goltsman E., Selkov E. , Elzer P.H., Hagius S., O'Callaghan D., Letesson J.-J., Haselkorn R., Kyrpides N.C., Overbeek R. Proc. Natl. Acad. Sci. U.S.A. 99:443-448(2002) [PubMed: 11756688] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: 16M / ATCC 23456 / NCTC 10094.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AE008918 Genomic DNA. Translation: AAL53514.1. Different initiation.
PIR	AG3543.
RefSeq	NP_541250.1. NC_003318.1.
3D structure databases
ProteinModelPortal	Q8YDA5.
ModBase	Search...
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	1198044.
GenomeReviews	Gene locus BMEII0273 in contig AE008918_GR.
KEGG	bme:BMEII0273.
PATRIC	17855184. VBIBruMel92729_2563.
Organism-specific databases
CMR	Search...
Phylogenomic databases
HOGENOM	HBG497643.
OMA	GGNAMID.
PhylomeDB	Q8YDA5.
ProtClustDB	PRK00942.
Enzyme and pathway databases
BioCyc	BMEL224914:BMEII0273-MONOMER.
Family and domain databases
HAMAP	MF_00082_B. ArgB_B. [Tree]
InterPro	IPR004662. AcgluKinase. IPR001048. Asp/Glu/Uridylate_kinase. [Graphical view]
Gene3D	G3DSA:3.40.1160.10. Aa_kinase. 1 hit.
KO	K00930.
Pfam	PF00696. AA_kinase. 1 hit. [Graphical view]
PIRSF	PIRSF000728. NAGK. 1 hit.
SUPFAM	SSF53633. Aa_kinase. 1 hit.
TIGRFAMs	TIGR00761. ArgB. 1 hit.
ProtoNet	Search...

Entry information

Entry name

ARGB_BRUME

Accession

Primary (citable) accession number: Q8YDA5

Entry history

Integrated into UniProtKB/Swiss-Prot:	June 20, 2002
Last sequence update:	November 7, 2003
Last modified:	January 25, 2012
This is version 66 of the entry and version 3 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program