Q8YD40 (METN_BRUME) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Methionine import ATP-binding protein MetN
EC=3.6.3.-

Gene names

Name:	metN
Ordered Locus Names:	BMEII0337

Organism

Brucella melitensis biotype 1 (strain 16M / ATCC 23456 / NCTC 10094) [Complete proteome] [HAMAP]

Taxonomic identifier

224914 [NCBI]

Taxonomic lineage

cellular organisms › Bacteria › Proteobacteria › Alphaproteobacteria › Rhizobiales › Brucellaceae › Brucella › Brucella melitensis › Brucella melitensis bv. 1

Protein attributes

Sequence length	369 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Part of the ABC transporter complex MetNIQ involved in methionine import. Responsible for energy coupling to the transport system By similarity.
Subunit structure	The complex is composed of two ATP-binding proteins (MetN), two transmembrane proteins (MetI) and a solute-binding protein (MetQ) Potential.
Subcellular location	Cell inner membrane; Peripheral membrane protein By similarity.
Sequence similarities	Belongs to the ABC transporter superfamily. Methionine importer (TC 3.A.1.24) family. [View classification] Contains 1 ABC transporter domain.

Ontologies

Keywords
Biological process	Amino-acid transport Transport
Cellular component	Cell inner membrane Cell membrane Membrane
Ligand	ATP-binding Nucleotide-binding
Molecular function	Hydrolase
Technical term	Complete proteome
Gene Ontology (GO)
Biological_process	ATP catabolic process Inferred from electronic annotation. Source: GOC
Cellular_component	plasma membrane Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular_function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW ATPase activity Inferred from electronic annotation. Source: InterPro methionine transmembrane transporter activity Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 369	369	Methionine import ATP-binding protein MetN		PRO_0000270259
Regions
	Domain	31 – 266	236	ABC transporter
	Nucleotide binding	63 – 70	8	ATP Potential

Sequences

	Sequence		Length	Mass (Da)
	Q8YD40 [UniParc]. Last modified March 1, 2002. Version 1. Checksum: 0E42048280DB72DD	FASTA	369	39,445
	10 20 30 40 50 60 MQHAAVCSGT SRHRRTTVTI EKPPATSAPI VEMKDVRRMF GETAAINGVS LSVARGEILG 70 80 90 100 110 120 IIGRSGAGKS TLIRCVNGLE KPDTGSIHIE GREITSLDED ALRPVRRRIG MVFQHFNLLS 130 140 150 160 170 180 AKTAAQNIAL PLKIAGMPKA ERIKRVAELL ELVGLSDKAS HYPAQLSGGQ KQRVGIARAL 190 200 210 220 230 240 AAEPAVLLSD EATSALDPET TQSILALLKD INAKLGLTIL LITHEMDVIR RIADRVIVLD 250 260 270 280 290 300 HGLIAEEGPV WKVFANPQSP VTQSMLQVLT PELPAIWRNR LEKKGDQAIL RVKLSGMAAK 310 320 330 340 350 360 GAFFNDVAAA TSLAPQLIHG GMDTIQGEPV GTLFIGLPAE DKTKLKAAIG YLNTHADATE VLGYVSGNA

References

[1]

"The genome sequence of the facultative intracellular pathogen Brucella melitensis." DelVecchio V.G., Kapatral V., Redkar R.J., Patra G., Mujer C., Los T., Ivanova N., Anderson I., Bhattacharyya A., Lykidis A., Reznik G., Jablonski L., Larsen N., D'Souza M., Bernal A., Mazur M., Goltsman E., Selkov E. , Elzer P.H., Hagius S., O'Callaghan D., Letesson J.-J., Haselkorn R., Kyrpides N.C., Overbeek R. Proc. Natl. Acad. Sci. U.S.A. 99:443-448(2002) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: 16M / ATCC 23456 / NCTC 10094.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AE008918 Genomic DNA. Translation: AAL53579.1.
PIR	AH3551.
RefSeq	NP_541315.1. NC_003318.1.
3D structure databases
HSSP	HSSP built from PDB template 1B0U based on UniProtKB P02915.
ProteinModelPortal	Q8YD40.
ModBase	Search...
Protein-protein interaction databases
STRING	224914.BMEII0337.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblBacteria	AAL53579; AAL53579; BMEII0337.
GeneID	1198109.
KEGG	bme:BMEII0337.
PATRIC	17855329. VBIBruMel92729_2633.
Organism-specific databases
CMR	Search...
Phylogenomic databases
KO	K02071.
OMA	GGIDHIQ.
ProtClustDB	CLSK864122.
Family and domain databases
InterPro	IPR003593. AAA+_ATPase. IPR026253. ABC_MetN. IPR003439. ABC_transporter-like. IPR017871. ABC_transporter_CS. IPR017908. ABC_transprt_methionine_MetN_C. IPR018449. NIL_domain. [Graphical view]
PANTHER	PTHR24220:SF186. PTHR24220:SF186. 1 hit.
Pfam	PF00005. ABC_tran. 1 hit. PF09383. NIL. 1 hit. [Graphical view]
SMART	SM00382. AAA. 1 hit. SM00930. NIL. 1 hit. [Graphical view]
PROSITE	PS00211. ABC_TRANSPORTER_1. 1 hit. PS50893. ABC_TRANSPORTER_2. 1 hit. PS51264. METN. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

METN_BRUME

Accession

Primary (citable) accession number: Q8YD40

Entry history

Integrated into UniProtKB/Swiss-Prot:	January 9, 2007
Last sequence update:	March 1, 2002
Last modified:	May 1, 2013
This is version 84 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program