Q8YD03 (ALR_BRUME) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Alanine racemase
EC=5.1.1.1

Gene names

Name:	alr
Ordered Locus Names:	BMEII0374

Organism

Brucella melitensis biotype 1 (strain 16M / ATCC 23456 / NCTC 10094) [Complete proteome] [HAMAP]

Taxonomic identifier

224914 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Alphaproteobacteria › Rhizobiales › Brucellaceae › Brucella

Protein attributes

Sequence length	396 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Provides the D-alanine required for cell wall biosynthesis By similarity. HAMAP MF_01201
Catalytic activity	L-alanine = D-alanine. HAMAP MF_01201
Cofactor	Pyridoxal phosphate By similarity. HAMAP MF_01201
Pathway	Amino-acid biosynthesis; D-alanine biosynthesis; D-alanine from L-alanine: step 1/1. HAMAP MF_01201 Cell wall biogenesis; peptidoglycan biosynthesis. HAMAP MF_01201
Sequence similarities	Belongs to the alanine racemase family.
Sequence caution	The sequence AAL53616.1 differs from that shown. Reason: Erroneous initiation.

Ontologies

Keywords
Biological process	Cell shape Cell wall biogenesis/degradation Peptidoglycan synthesis
Ligand	Pyridoxal phosphate
Molecular function	Isomerase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	alanine metabolic process Inferred from electronic annotation. Source: InterPro cellular cell wall organization Inferred from electronic annotation. Source: UniProtKB-KW peptidoglycan biosynthetic process Inferred from electronic annotation. Source: UniProtKB-KW regulation of cell shape Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	alanine racemase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 396	396	Alanine racemase HAMAP MF_01201		PRO_0000114503
Sites
	Active site	46	1	Proton acceptor; specific for D-alanine By similarity
	Active site	280	1	Proton acceptor; specific for L-alanine By similarity
Amino acid modifications
	Modified residue	46	1	N6-(pyridoxal phosphate)lysine By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q8YD03 [UniParc]. Last modified May 10, 2002. Version 2. Checksum: 3BA110919EB0066A	FASTA	396	42,368
	10 20 30 40 50 60 MSLPFSQDER DLAAGGILTI DLAALRHNYS AIATRIAPTR TAAVVKADAY GLGASRVAPA 70 80 90 100 110 120 FYEAGCRDFF VAHLGEAVAL KPFLKPDATL YVLNGLQPGT EAACAREGIL PVLNSLEQVE 130 140 150 160 170 180 NWAALATRLG KKLPALLQFD TGMSRLGLSA KEFDRLLENV TLLSRIDIKF AISHLANGDE 190 200 210 220 230 240 PGNAVNARQL AKMTALLARL PKLPAALANS GGTFLGKTYY FDLARPGIAL YGIDPERQHD 250 260 270 280 290 300 FSDKVAHENK KPKHSILPVL TLSARVIQVR DVDKGATVGY GGTYVANGPM RIATIAVGYA 310 320 330 340 350 360 DGLFRSLSNK GAAFFGDTRL PIIGRVSMDS ITLDVTSLPE GTLKLGSLVE LIGPHQRLED 370 380 390 VARDCDTIPY EILTALGNRY ARVYVYVNGG GTSTTA

References

[1]

"The genome sequence of the facultative intracellular pathogen Brucella melitensis." DelVecchio V.G., Kapatral V., Redkar R.J., Patra G., Mujer C., Los T., Ivanova N., Anderson I., Bhattacharyya A., Lykidis A., Reznik G., Jablonski L., Larsen N., D'Souza M., Bernal A., Mazur M., Goltsman E., Selkov E. , Elzer P.H., Hagius S., O'Callaghan D., Letesson J.-J., Haselkorn R., Kyrpides N.C., Overbeek R. Proc. Natl. Acad. Sci. U.S.A. 99:443-448(2002) [PubMed: 11756688] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: 16M / ATCC 23456 / NCTC 10094.

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Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AE008918 Genomic DNA. Translation: AAL53616.1. Different initiation.
PIR	AE3556.
RefSeq	NP_541352.1. NC_003318.1.
3D structure databases
ProteinModelPortal	Q8YD03.
ModBase	Search...
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	1198146.
GenomeReviews	Gene locus BMEII0374 in contig AE008918_GR.
KEGG	bme:BMEII0374.
PATRIC	17800388. VBIBruMel146950_2526.
Organism-specific databases
CMR	Search...
Phylogenomic databases
HOGENOM	HBG712172.
OMA	ILFIMSH.
PhylomeDB	Q8YD03.
ProtClustDB	CLSK898810.
Enzyme and pathway databases
BioCyc	BMEL224914:BMEII0374-MONOMER.
Family and domain databases
HAMAP	MF_01201. Ala_racemase. [Tree]
InterPro	IPR000821. Ala_racemase. IPR009006. Ala_racemase/Decarboxylase_C. IPR011079. Ala_racemase_C. IPR001608. Ala_racemase_N. IPR020622. Ala_racemase_pyridoxalP-BS. [Graphical view]
Gene3D	G3DSA:2.40.37.10. Ala_racemase/Decarboxylase_C. 1 hit.
KO	K01775.
Pfam	PF00842. Ala_racemase_C. 1 hit. PF01168. Ala_racemase_N. 1 hit. [Graphical view]
PRINTS	PR00992. ALARACEMASE.
SMART	SM01005. Ala_racemase_C. 1 hit. [Graphical view]
SUPFAM	SSF50621. Racem_decarbox_C. 1 hit.
TIGRFAMs	TIGR00492. Alr. 1 hit.
PROSITE	PS00395. ALANINE_RACEMASE. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

ALR_BRUME

Accession

Primary (citable) accession number: Q8YD03

Entry history

Integrated into UniProtKB/Swiss-Prot:	May 10, 2002
Last sequence update:	May 10, 2002
Last modified:	January 25, 2012
This is version 71 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program