Reviewed, UniProtKB/Swiss-Prot Q8YCV3 (TPIS2_BRUME)

Names and origin

Protein names

Recommended name:
    Triosephosphate isomerase 2
      Short name=TIM 2
    EC=5.3.1.1
Alternative name(s):
    Triose-phosphate isomerase 2

Gene names

Name:	tpiA2
Ordered Locus Names:	BMEII0425

Organism

Brucella melitensis [Complete proteome] [HAMAP]

Taxonomic identifier

29459 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Alphaproteobacteria › Rhizobiales › Brucellaceae › Brucella

Protein attributes

Sequence length	256 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Inferred from homology.

General annotation (Comments)

Catalytic activity	D-glyceraldehyde 3-phosphate = glycerone phosphate. HAMAP MF_00147
Pathway	Carbohydrate biosynthesis; gluconeogenesis. HAMAP MF_00147 Carbohydrate degradation; glycolysis; D-glyceraldehyde 3-phosphate from glycerone phosphate: step 1/1. HAMAP MF_00147
Subunit structure	Homodimer By similarity.
Subcellular location	Cytoplasm Probable.
Sequence similarities	Belongs to the triosephosphate isomerase family.

Ontologies

Keywords
Biological process	Gluconeogenesis Glycolysis Pentose shunt
Cellular component	Cytoplasm
Molecular function	Isomerase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	gluconeogenesis Inferred from electronic annotation. Source: HAMAP glycolysis Inferred from electronic annotation. Source: HAMAP pentose-phosphate shunt Inferred from electronic annotation. Source: HAMAP
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	triose-phosphate isomerase activity Inferred from electronic annotation. Source: HAMAP
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 256	256	Triosephosphate isomerase 2 HAMAP MF_00147		PRO_0000090192
Sites
	Active site	96	1	Electrophile By similarity
	Active site	169	1	Proton acceptor By similarity
	Binding site	11	1	Substrate By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q8YCV3-1 [UniParc]. Last modified March 1, 2002. Version 1. Checksum: 89F049D4B1306707	FASTA	256	27,848
	10 20 30 40 50 60 MTKFWIGTSW KMNKTLAEAR LFAEALKAAD AGRSPDIQRF VIPPFTAVRE VKEILSGTSV 70 80 90 100 110 120 KVGAQNMHWA DQGTWTGEIS PLMLKDCNLD IVELGHSERR EHFGETNETV GLKVEAAVRH 130 140 150 160 170 180 GLIPLICIGE TLEDCESGRA AAVLEEEVRG ALSKLSEAQK QAEILFAYEP VWAIGENGIP 190 200 210 220 230 240 ASADYADARQ AEIIAVAQSV LARRVPCLYG GSVNPGNCEE LIACPHIDGL FIGRSAWNVE 250 GYLDILARCA TKVQAN

References

[1]

"The genome sequence of the facultative intracellular pathogen Brucella melitensis." DelVecchio V.G., Kapatral V., Redkar R.J., Patra G., Mujer C., Los T., Ivanova N., Anderson I., Bhattacharyya A., Lykidis A., Reznik G., Jablonski L., Larsen N., D'Souza M., Bernal A., Mazur M., Goltsman E., Selkov E. , Elzer P.H., Hagius S., O'Callaghan D., Letesson J.-J., Haselkorn R., Kyrpides N.C., Overbeek R. Proc. Natl. Acad. Sci. U.S.A. 99:443-448(2002) [PubMed: 11756688] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: 16M / ATCC 23456 / NCTC 10094 / Biotype 1.

Cross-references

Sequence databases
EMBLGenBankDDBJ	AE008918 Genomic DNA. Translation: AAL53667.1.
PIR	AH3562.
RefSeq	NP_541403.1.
3D structure databases
HSSP	HSSP built from PDB template 2BTM based on UniProtKB P00943.
ModBase	Search...
Genome annotation databases
GeneID	1198197.
GenomeReviews	Gene locus BMEII0425 in contig AE008918_GR.
KEGG	bme:BMEII0425.
NMPDR	fig|224914.1.peg.2485.
Organism-specific databases
CMR	Search...
Phylogenomic databases
HOGENOM	Q8YCV3.
OMA	EHFGETD.
Enzyme and pathway databases
BioCyc	BMEL224914:BMEII0425-MON.
BRENDA	5.3.1.1. 277959.
Family and domain databases
HAMAP	MF_00147. [Tree]
InterPro	IPR013785. Aldolase_TIM. IPR000652. Triosephosphate_isomerase. [Graphical view]
Gene3D	G3DSA:3.20.20.70. Aldolase_TIM. 1 hit.
PANTHER	PTHR21139. Triophos_ismrse. 1 hit.
Pfam	PF00121. TIM. 1 hit. [Graphical view]
ProDom	PD001005. Triophos_ismrse. 1 hit. [Graphical view] [Entries sharing at least one domain]
TIGRFAMs	TIGR00419. tim. 1 hit.
PROSITE	PS00171. TIM_1. 1 hit. PS51440. TIM_2. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

TPIS2_BRUME

Accession

Primary (citable) accession number: Q8YCV3

Entry history

Integrated into UniProtKB/Swiss-Prot:	December 15, 2003
Last sequence update:	March 1, 2002
Last modified:	November 3, 2009
This is version 48 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)