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Q8YC48

- GLMU_BRUME

UniProt

Q8YC48 - GLMU_BRUME

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Protein

Bifunctional protein GlmU

Gene

glmU

Organism
Brucella melitensis biotype 1 (strain 16M / ATCC 23456 / NCTC 10094)
Status
Reviewed - Annotation score: 5 out of 5- Protein inferred from homologyi

Functioni

Catalyzes the last two sequential reactions in the de novo biosynthetic pathway for UDP-N-acetylglucosamine (UDP-GlcNAc). The C-terminal domain catalyzes the transfer of acetyl group from acetyl coenzyme A to glucosamine-1-phosphate (GlcN-1-P) to produce N-acetylglucosamine-1-phosphate (GlcNAc-1-P), which is converted into UDP-GlcNAc by the transfer of uridine 5-monophosphate (from uridine 5-triphosphate), a reaction catalyzed by the N-terminal domain.UniRule annotation

Catalytic activityi

Acetyl-CoA + alpha-D-glucosamine 1-phosphate = CoA + N-acetyl-alpha-D-glucosamine 1-phosphate.UniRule annotation
UTP + N-acetyl-alpha-D-glucosamine 1-phosphate = diphosphate + UDP-N-acetyl-alpha-D-glucosamine.UniRule annotation

Cofactori

Binds 1 magnesium ion per subunit.UniRule annotation

Pathwayi

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei25 – 251UDP-GlcNAcUniRule annotation
Binding sitei78 – 781UDP-GlcNAcUniRule annotation
Metal bindingi108 – 1081MagnesiumUniRule annotation
Binding sitei144 – 1441UDP-GlcNAc; via amide nitrogenUniRule annotation
Binding sitei158 – 1581UDP-GlcNAcUniRule annotation
Binding sitei173 – 1731UDP-GlcNAcUniRule annotation
Metal bindingi230 – 2301MagnesiumUniRule annotation
Binding sitei230 – 2301UDP-GlcNAcUniRule annotation
Binding sitei319 – 3191Acetyl-CoA; amide nitrogenUniRule annotation
Binding sitei337 – 3371Acetyl-CoAUniRule annotation
Active sitei349 – 3491Proton acceptorUniRule annotation
Binding sitei352 – 3521Acetyl-CoAUniRule annotation
Binding sitei363 – 3631Acetyl-CoAUniRule annotation
Binding sitei391 – 3911Acetyl-CoAUniRule annotation
Binding sitei409 – 4091Acetyl-CoA; via amide nitrogenUniRule annotation
Binding sitei426 – 4261Acetyl-CoAUniRule annotation

GO - Molecular functioni

  1. glucosamine-1-phosphate N-acetyltransferase activity Source: UniProtKB-HAMAP
  2. magnesium ion binding Source: UniProtKB-HAMAP
  3. UDP-N-acetylglucosamine diphosphorylase activity Source: UniProtKB-HAMAP

GO - Biological processi

  1. cell morphogenesis Source: UniProtKB-HAMAP
  2. cell wall organization Source: UniProtKB-KW
  3. lipid A biosynthetic process Source: UniProtKB-UniPathway
  4. lipopolysaccharide biosynthetic process Source: InterPro
  5. peptidoglycan biosynthetic process Source: UniProtKB-HAMAP
  6. regulation of cell shape Source: UniProtKB-KW
  7. UDP-N-acetylglucosamine biosynthetic process Source: UniProtKB-UniPathway
Complete GO annotation...

Keywords - Molecular functioni

Acyltransferase, Nucleotidyltransferase, Transferase

Keywords - Biological processi

Cell shape, Cell wall biogenesis/degradation, Peptidoglycan synthesis

Keywords - Ligandi

Magnesium, Metal-binding

Enzyme and pathway databases

BioCyciBMEL224914:GCJ0-2808-MONOMER.
UniPathwayiUPA00113; UER00532.
UPA00113; UER00533.
UPA00973.

Names & Taxonomyi

Protein namesi
Recommended name:
Bifunctional protein GlmUUniRule annotation
Including the following 2 domains:
UDP-N-acetylglucosamine pyrophosphorylaseUniRule annotation (EC:2.7.7.23UniRule annotation)
Alternative name(s):
N-acetylglucosamine-1-phosphate uridyltransferaseUniRule annotation
Glucosamine-1-phosphate N-acetyltransferaseUniRule annotation (EC:2.3.1.157UniRule annotation)
Gene namesi
Name:glmUUniRule annotation
Ordered Locus Names:BMEII0684
OrganismiBrucella melitensis biotype 1 (strain 16M / ATCC 23456 / NCTC 10094)
Taxonomic identifieri224914 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaAlphaproteobacteriaRhizobialesBrucellaceaeBrucella
ProteomesiUP000000419: Chromosome II

Subcellular locationi

Cytoplasm UniRule annotation

GO - Cellular componenti

  1. cytoplasm Source: UniProtKB-HAMAP
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 454454Bifunctional protein GlmUPRO_0000233745Add
BLAST

Interactioni

Subunit structurei

Homotrimer.UniRule annotation

Protein-protein interaction databases

STRINGi224914.BMEII0684.

Structurei

3D structure databases

ProteinModelPortaliQ8YC48.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni1 – 232232PyrophosphorylaseUniRule annotationAdd
BLAST
Regioni11 – 144UDP-GlcNAc bindingUniRule annotation
Regioni83 – 842UDP-GlcNAc bindingUniRule annotation
Regioni233 – 25321LinkerUniRule annotationAdd
BLAST
Regioni254 – 454201N-acetyltransferaseUniRule annotationAdd
BLAST
Regioni372 – 3732Acetyl-CoA bindingUniRule annotation

Sequence similaritiesi

In the N-terminal section; belongs to the N-acetylglucosamine-1-phosphate uridyltransferase family.UniRule annotation
In the C-terminal section; belongs to the transferase hexapeptide repeat family.UniRule annotation

Keywords - Domaini

Repeat

Phylogenomic databases

HOGENOMiHOG000283476.
KOiK04042.
OMAiIGERAYI.
OrthoDBiEOG6Z6FQZ.

Family and domain databases

Gene3Di3.90.550.10. 1 hit.
HAMAPiMF_01631. GlmU.
InterProiIPR005882. Bifunctional_GlmU.
IPR001451. Hexapep_transf.
IPR018357. Hexapep_transf_CS.
IPR025877. MobA-like_NTP_Trfase_dom.
IPR029044. Nucleotide-diphossugar_trans.
IPR011004. Trimer_LpxA-like.
[Graphical view]
PfamiPF00132. Hexapep. 4 hits.
PF12804. NTP_transf_3. 1 hit.
[Graphical view]
SUPFAMiSSF51161. SSF51161. 1 hit.
SSF53448. SSF53448. 1 hit.
TIGRFAMsiTIGR01173. glmU. 1 hit.
PROSITEiPS00101. HEXAPEP_TRANSFERASES. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q8YC48-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MTDRTCLSIV LAAGEGTRMK SNLPKVLHRV AGLPLVCHVV NAVRGTGKSD
60 70 80 90 100
VALVVGRGAE DVRSAVEKIA GPVSAFEQKE RLGTAHAVLA AREAIARGYD
110 120 130 140 150
DLLIVFGDTP LIEAQSLLAA RERLAQGADL VVIGFRPASP HGYGRLIEEG
160 170 180 190 200
GQLVAIIEEK EATDEQKKIG FCNGGLMALR GQHALALLDA VGNDNAKGEY
210 220 230 240 250
YLTDIVAIAH GKGLNVTAIE VPVDNVIGIN NRAELAEAET IWQNRKRREL
260 270 280 290 300
MLSGVTLIAP ETVFFSYDTV IEPDVVIEPN VFFGPSVHVA SGALIHSFSH
310 320 330 340 350
LEGAQVGEKA EIGPFARLRP GADLAEKSKV GNFCEVKNAK VGKGAKINHL
360 370 380 390 400
AYIGDAVIGA SSNIGAGTIT CNYDGYNKFK TIIGDNAFIG SNSSLVAPVE
410 420 430 440 450
IGDNAYIASG SVITADVPAD ALALGRARQE TKEGRAKILR EKYAAIKAAK

SVSK
Length:454
Mass (Da):47,905
Last modified:May 2, 2006 - v2
Checksum:i0D9A7E45C22E2A05
GO

Sequence cautioni

The sequence AAL53926.1 differs from that shown. Reason: Erroneous initiation.

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AE008918 Genomic DNA. Translation: AAL53926.1. Different initiation.
PIRiAC3595.
RefSeqiNP_541662.1. NC_003318.1.

Genome annotation databases

EnsemblBacteriaiAAL53926; AAL53926; BMEII0684.
GeneIDi1198456.
KEGGibme:BMEII0684.
PATRICi17856108. VBIBruMel92729_3016.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AE008918 Genomic DNA. Translation: AAL53926.1 . Different initiation.
PIRi AC3595.
RefSeqi NP_541662.1. NC_003318.1.

3D structure databases

ProteinModelPortali Q8YC48.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

STRINGi 224914.BMEII0684.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblBacteriai AAL53926 ; AAL53926 ; BMEII0684 .
GeneIDi 1198456.
KEGGi bme:BMEII0684.
PATRICi 17856108. VBIBruMel92729_3016.

Phylogenomic databases

HOGENOMi HOG000283476.
KOi K04042.
OMAi IGERAYI.
OrthoDBi EOG6Z6FQZ.

Enzyme and pathway databases

UniPathwayi UPA00113 ; UER00532 .
UPA00113 ; UER00533 .
UPA00973 .
BioCyci BMEL224914:GCJ0-2808-MONOMER.

Family and domain databases

Gene3Di 3.90.550.10. 1 hit.
HAMAPi MF_01631. GlmU.
InterProi IPR005882. Bifunctional_GlmU.
IPR001451. Hexapep_transf.
IPR018357. Hexapep_transf_CS.
IPR025877. MobA-like_NTP_Trfase_dom.
IPR029044. Nucleotide-diphossugar_trans.
IPR011004. Trimer_LpxA-like.
[Graphical view ]
Pfami PF00132. Hexapep. 4 hits.
PF12804. NTP_transf_3. 1 hit.
[Graphical view ]
SUPFAMi SSF51161. SSF51161. 1 hit.
SSF53448. SSF53448. 1 hit.
TIGRFAMsi TIGR01173. glmU. 1 hit.
PROSITEi PS00101. HEXAPEP_TRANSFERASES. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

  1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: 16M / ATCC 23456 / NCTC 10094.

Entry informationi

Entry nameiGLMU_BRUME
AccessioniPrimary (citable) accession number: Q8YC48
Entry historyi
Integrated into UniProtKB/Swiss-Prot: May 2, 2006
Last sequence update: May 2, 2006
Last modified: October 29, 2014
This is version 92 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Multifunctional enzyme

Documents

  1. Brucella melitensis
    Brucella melitensis (strain 16M): entries and gene names
  2. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  3. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3