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Protein

Glutamine--fructose-6-phosphate aminotransferase [isomerizing]

Gene

glmS

Organism
Brucella melitensis biotype 1 (strain 16M / ATCC 23456 / NCTC 10094)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Protein inferred from homologyi

Functioni

Catalyzes the first step in hexosamine metabolism, converting fructose-6P into glucosamine-6P using glutamine as a nitrogen source.

Catalytic activityi

L-glutamine + D-fructose 6-phosphate = L-glutamate + D-glucosamine 6-phosphate.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Active sitei2Nucleophile; for GATase activity1
Active sitei602For Fru-6P isomerization activity1

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionAminotransferase, Transferase

Names & Taxonomyi

Protein namesi
Recommended name:
Glutamine--fructose-6-phosphate aminotransferase [isomerizing] (EC:2.6.1.16)
Alternative name(s):
D-fructose-6-phosphate amidotransferase
GFAT
Glucosamine-6-phosphate synthase
Hexosephosphate aminotransferase
L-glutamine--D-fructose-6-phosphate amidotransferase
Gene namesi
Name:glmS
Ordered Locus Names:BMEII0685
OrganismiBrucella melitensis biotype 1 (strain 16M / ATCC 23456 / NCTC 10094)
Taxonomic identifieri224914 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaAlphaproteobacteriaRhizobialesBrucellaceaeBrucella
Proteomesi
  • UP000000419 Componenti: Chromosome II

Subcellular locationi

  • Cytoplasm

GO - Cellular componenti

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Initiator methionineiRemoved
ChainiPRO_00001353092 – 607Glutamine--fructose-6-phosphate aminotransferase [isomerizing]Add BLAST606

Interactioni

Subunit structurei

Homodimer.

Protein-protein interaction databases

STRINGi224914.BAWG_1888.

Structurei

3D structure databases

ProteinModelPortaliQ8YC47.
SMRiQ8YC47.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini2 – 217Glutamine amidotransferase type-2Add BLAST216
Domaini283 – 422SIS 1Add BLAST140
Domaini455 – 597SIS 2Add BLAST143

Keywords - Domaini

Glutamine amidotransferase, Repeat

Phylogenomic databases

eggNOGiENOG4105C46. Bacteria.
COG0449. LUCA.
HOGENOMiHOG000258896.
KOiK00820.
OMAiASEYRYA.

Family and domain databases

CDDicd05008. SIS_GlmS_GlmD_1. 1 hit.
cd05009. SIS_GlmS_GlmD_2. 1 hit.
Gene3Di3.60.20.10. 1 hit.
HAMAPiMF_00164. GlmS. 1 hit.
InterProiView protein in InterPro
IPR017932. GATase_2_dom.
IPR035466. GlmS/AgaS_SIS.
IPR035490. GlmS/FrlB_SIS.
IPR005855. GlmS_trans.
IPR029055. Ntn_hydrolases_N.
IPR001347. SIS.
PANTHERiPTHR10937:SF0. PTHR10937:SF0. 1 hit.
PfamiView protein in Pfam
PF01380. SIS. 2 hits.
SUPFAMiSSF56235. SSF56235. 1 hit.
TIGRFAMsiTIGR01135. glmS. 1 hit.
PROSITEiView protein in PROSITE
PS51278. GATASE_TYPE_2. 1 hit.
PS51464. SIS. 2 hits.

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

Q8YC47-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MCGIIGIIGN DEVAPRLVDA LKRLEYRGYD SAGIATLQNG RLDRRRAEGK
60 70 80 90 100
LVNLEKRLAG EPLPGVIGIG HTRWATHGRP VEHNAHPHIT TRLAVVHNGI
110 120 130 140 150
IENFAELRAM LEAEGRKFET ETDTEAVAHL VTRELEKGKS PVEAVRDCLP
160 170 180 190 200
HLKGAFALAF LFEGDEELLI GARQGPPLAV GYGEGEMFLG SDAIALAPFT
210 220 230 240 250
DTISYLEDGD WAVLTRNGVS IYDENNKPVE RPVQKSQNTN MLVSKGNHRH
260 270 280 290 300
FMQKEMFEQP EVISHTLANY LDFTTGKVRK EAIGIDFSKV DRLTITACGT
310 320 330 340 350
AYYAATVAKY WFEQIARLPV DSDIASEFRY REMPLSKDSL AMFVSQSGET
360 370 380 390 400
ADTLASLRYC KAQGLKIASV LNVTGSTIAR ESDAVFPTLA GPEIGVASTK
410 420 430 440 450
AFTCQLSAMA SLAIAAARAR GAIDEVREQE LVHQLSEAPR FINQVLKLED
460 470 480 490 500
QIAVVCHDLS KVNHVLYLGR GTSFPLAMEG ALKLKEISYI HAEGYAAGEL
510 520 530 540 550
KHGPIALIDE TMPVIVIAPS DRLYEKTVSN MQEVAARGGR IILITDKKGA
560 570 580 590 600
ESASIDTMAT IVLPEVPEFI SPLVYALPIQ MLAYHTAVLM GTDVDQPRNL

AKSVTVE
Length:607
Mass (Da):66,330
Last modified:January 23, 2007 - v4
Checksum:i8DFBB72BC3DB000F
GO

Sequence cautioni

The sequence AAL53927 differs from that shown. Reason: Erroneous initiation.Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AE008918 Genomic DNA. Translation: AAL53927.1. Different initiation.
PIRiAD3595.
RefSeqiWP_004684961.1. NZ_GG703779.1.

Genome annotation databases

EnsemblBacteriaiAAL53927; AAL53927; BMEII0685.
GeneIDi29595126.
KEGGibme:BMEII0685.
bmel:DK63_2556.
PATRICifig|224914.52.peg.2679.

Similar proteinsi

Entry informationi

Entry nameiGLMS_BRUME
AccessioniPrimary (citable) accession number: Q8YC47
Entry historyiIntegrated into UniProtKB/Swiss-Prot: July 11, 2002
Last sequence update: January 23, 2007
Last modified: October 25, 2017
This is version 103 of the entry and version 4 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome

Documents

  1. Brucella melitensis
    Brucella melitensis (strain 16M): entries and gene names