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Protein

Ribosomal protein S12 methylthiotransferase RimO

Gene

rimO

Organism
Brucella melitensis biotype 1 (strain 16M / ATCC 23456 / NCTC 10094)
Status
Reviewed-Annotation score: -Protein inferred from homologyi

Functioni

Catalyzes the methylthiolation of an aspartic acid residue of ribosomal protein S12.UniRule annotation

Catalytic activityi

L-aspartate-[ribosomal protein S12] + sulfur-(sulfur carrier) + 2 S-adenosyl-L-methionine = 3-methylthio-L-aspartate-[ribosomal protein S12] + S-adenosyl-L-homocysteine + (sulfur carrier) + L-methionine + 5'-deoxyadenosine.UniRule annotation

Cofactori

[4Fe-4S] clusterUniRule annotationNote: Binds 2 [4Fe-4S] clusters. One cluster is coordinated with 3 cysteines and an exchangeable S-adenosyl-L-methionine.UniRule annotation

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Metal bindingi13Iron-sulfur (4Fe-4S)UniRule annotation1
Metal bindingi49Iron-sulfur (4Fe-4S)UniRule annotation1
Metal bindingi78Iron-sulfur (4Fe-4S)UniRule annotation1
Metal bindingi145Iron-sulfur (4Fe-4S-S-AdoMet)UniRule annotation1
Metal bindingi149Iron-sulfur (4Fe-4S-S-AdoMet)UniRule annotation1
Metal bindingi152Iron-sulfur (4Fe-4S-S-AdoMet)UniRule annotation1

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionTransferase
Ligand4Fe-4S, Iron, Iron-sulfur, Metal-binding, S-adenosyl-L-methionine

Names & Taxonomyi

Protein namesi
Recommended name:
Ribosomal protein S12 methylthiotransferase RimOUniRule annotation (EC:2.8.4.4UniRule annotation)
Short name:
S12 MTTaseUniRule annotation
Short name:
S12 methylthiotransferaseUniRule annotation
Alternative name(s):
Ribosomal protein S12 (aspartate-C(3))-methylthiotransferaseUniRule annotation
Ribosome maturation factor RimOUniRule annotation
Gene namesi
Name:rimOUniRule annotation
Ordered Locus Names:BMEII0703
OrganismiBrucella melitensis biotype 1 (strain 16M / ATCC 23456 / NCTC 10094)
Taxonomic identifieri224914 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaAlphaproteobacteriaRhizobialesBrucellaceaeBrucella
Proteomesi
  • UP000000419 Componenti: Chromosome II

Subcellular locationi

  • Cytoplasm UniRule annotation

GO - Cellular componenti

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00003747241 – 437Ribosomal protein S12 methylthiotransferase RimOAdd BLAST437

Interactioni

Protein-protein interaction databases

STRINGi224914.BAWG_1872

Structurei

3D structure databases

ProteinModelPortaliQ8YC29
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini4 – 114MTTase N-terminalUniRule annotationAdd BLAST111
Domaini372 – 437TRAMUniRule annotationAdd BLAST66

Sequence similaritiesi

Belongs to the methylthiotransferase family. RimO subfamily.UniRule annotation

Phylogenomic databases

eggNOGiENOG4105CBM Bacteria
COG0621 LUCA
HOGENOMiHOG000224766
KOiK14441
OMAiCLSERYK

Family and domain databases

Gene3Di3.40.50.12160, 1 hit
3.80.30.20, 1 hit
HAMAPiMF_01865 MTTase_RimO, 1 hit
InterProiView protein in InterPro
IPR006638 Elp3/MiaB/NifB
IPR005839 Methylthiotransferase
IPR020612 Methylthiotransferase_CS
IPR013848 Methylthiotransferase_N
IPR038135 Methylthiotransferase_N_sf
IPR005840 Ribosomal_S12_MeSTrfase_RimO
IPR007197 rSAM
IPR023404 rSAM_horseshoe
IPR002792 TRAM_dom
PANTHERiPTHR43837 PTHR43837, 1 hit
PfamiView protein in Pfam
PF04055 Radical_SAM, 1 hit
PF01938 TRAM, 1 hit
PF00919 UPF0004, 1 hit
SFLDiSFLDF00274 ribosomal_protein_S12_methylth, 1 hit
SFLDG01061 methylthiotransferase, 1 hit
SFLDS00029 Radical_SAM, 1 hit
SMARTiView protein in SMART
SM00729 Elp3, 1 hit
TIGRFAMsiTIGR00089 TIGR00089, 1 hit
TIGR01125 TIGR01125, 1 hit
PROSITEiView protein in PROSITE
PS51449 MTTASE_N, 1 hit
PS01278 MTTASE_RADICAL, 1 hit
PS50926 TRAM, 1 hit

Sequencei

Sequence statusi: Complete.

Q8YC29-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MSAPRVSFVS LGCPKALVDS ERIITGLRSE GYEISRKHDG ADLVIVNTCG
60 70 80 90 100
FLDSARDESL EAIGLALNEN GKVIVTGCLG AEPDVIRERH PNVLAITGPQ
110 120 130 140 150
AYESVMNAVH EVAPPAHDPF VDLVPPQGVK LTPRHYAYLK ISEGCSNRCS
160 170 180 190 200
FCIIPALRGD LVSRPINEVL REAEKLVQAG VKEILVISQD TSAYGLDIKY
210 220 230 240 250
QEAMWQDRTV RTKFLDLSRE LGEMGVWVRM HYVYPYPHVD EVIPLMAEGK
260 270 280 290 300
ILPYLDIPFQ HASPAVLKNM RRPAHQEKTS RRIQAWRETC PDLAVRSTFI
310 320 330 340 350
VGYPGETEED FQMLLDWLDE AKIERAGCFK YEAVKGAKAN DLGLEQVPEE
360 370 380 390 400
VKEARWHRFM AKQQQISTNL LKKKVGKRLP VIIDEANGTI GKGRTRYDAP
410 420 430
EIDGSVHISS RRPLRVGDIV TVKIEASDAY DLHGTAV
Length:437
Mass (Da):48,774
Last modified:March 1, 2002 - v1
Checksum:i530B6217DDF24656
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AE008918 Genomic DNA Translation: AAL53945.1
PIRiAF3597
RefSeqiWP_002966068.1, NZ_GG703779.1

Genome annotation databases

EnsemblBacteriaiAAL53945; AAL53945; BMEII0703
GeneIDi29595209
KEGGibme:BMEII0703
bmel:DK63_2539
PATRICifig|224914.52.peg.2661

Similar proteinsi

Entry informationi

Entry nameiRIMO_BRUME
AccessioniPrimary (citable) accession number: Q8YC29
Entry historyiIntegrated into UniProtKB/Swiss-Prot: May 26, 2009
Last sequence update: March 1, 2002
Last modified: March 28, 2018
This is version 103 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome

Documents

  1. Brucella melitensis
    Brucella melitensis (strain 16M): entries and gene names
  2. SIMILARITY comments
    Index of protein domains and families
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