Q8YC29 (RIMO_BRUME) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Ribosomal protein S12 methylthiotransferase RimO
Short name=S12 MTTase
Short name=S12 methylthiotransferase
EC=2.-.-.-

Alternative name(s):
Ribosome maturation factor RimO

Gene names

Name:	rimO
Ordered Locus Names:	BMEII0703

Organism

Brucella melitensis biotype 1 (strain 16M / ATCC 23456 / NCTC 10094) [Complete proteome] [HAMAP]

Taxonomic identifier

224914 [NCBI]

Taxonomic lineage

cellular organisms › Bacteria › Proteobacteria › Alphaproteobacteria › Rhizobiales › Brucellaceae › Brucella › Brucella melitensis › Brucella melitensis bv. 1

Protein attributes

Sequence length	437 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Catalyzes the methylthiolation of an aspartic acid residue of ribosomal protein S12 By similarity. HAMAP-Rule MF_01865
Cofactor	Binds 2 4Fe-4S clusters. One cluster is coordinated with 3 cysteines and an exchangeable S-adenosyl-L-methionine By similarity.
Subcellular location	Cytoplasm Potential HAMAP-Rule MF_01865.
Sequence similarities	Belongs to the methylthiotransferase family. RimO subfamily. Contains 1 MTTase N-terminal domain. Contains 1 TRAM domain.

Ontologies

Keywords
Cellular component	Cytoplasm
Ligand	4Fe-4S Iron Iron-sulfur Metal-binding S-adenosyl-L-methionine
Molecular function	Transferase
Technical term	Complete proteome
Gene Ontology (GO)
Biological_process	RNA modification Inferred from electronic annotation. Source: InterPro peptidyl-L-beta-methylthioaspartic acid biosynthetic process from peptidyl-aspartic acid Inferred from electronic annotation. Source: HAMAP
Cellular_component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular_function	4 iron, 4 sulfur cluster binding Inferred from electronic annotation. Source: HAMAP iron ion binding Inferred from electronic annotation. Source: HAMAP transferase activity Inferred from electronic annotation. Source: HAMAP
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 437	437	Ribosomal protein S12 methylthiotransferase RimO HAMAP-Rule MF_01865		PRO_0000374724
Regions
	Domain	4 – 114	111	MTTase N-terminal
	Domain	372 – 437	66	TRAM
Sites
	Metal binding	13	1	Iron-sulfur (4Fe-4S) By similarity
	Metal binding	49	1	Iron-sulfur (4Fe-4S) By similarity
	Metal binding	78	1	Iron-sulfur (4Fe-4S) By similarity
	Metal binding	145	1	Iron-sulfur (4Fe-4S-S-AdoMet) By similarity
	Metal binding	149	1	Iron-sulfur (4Fe-4S-S-AdoMet) By similarity
	Metal binding	152	1	Iron-sulfur (4Fe-4S-S-AdoMet) By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q8YC29 [UniParc]. Last modified March 1, 2002. Version 1. Checksum: 530B6217DDF24656	FASTA	437	48,774
	10 20 30 40 50 60 MSAPRVSFVS LGCPKALVDS ERIITGLRSE GYEISRKHDG ADLVIVNTCG FLDSARDESL 70 80 90 100 110 120 EAIGLALNEN GKVIVTGCLG AEPDVIRERH PNVLAITGPQ AYESVMNAVH EVAPPAHDPF 130 140 150 160 170 180 VDLVPPQGVK LTPRHYAYLK ISEGCSNRCS FCIIPALRGD LVSRPINEVL REAEKLVQAG 190 200 210 220 230 240 VKEILVISQD TSAYGLDIKY QEAMWQDRTV RTKFLDLSRE LGEMGVWVRM HYVYPYPHVD 250 260 270 280 290 300 EVIPLMAEGK ILPYLDIPFQ HASPAVLKNM RRPAHQEKTS RRIQAWRETC PDLAVRSTFI 310 320 330 340 350 360 VGYPGETEED FQMLLDWLDE AKIERAGCFK YEAVKGAKAN DLGLEQVPEE VKEARWHRFM 370 380 390 400 410 420 AKQQQISTNL LKKKVGKRLP VIIDEANGTI GKGRTRYDAP EIDGSVHISS RRPLRVGDIV 430 TVKIEASDAY DLHGTAV

References

[1]

"The genome sequence of the facultative intracellular pathogen Brucella melitensis." DelVecchio V.G., Kapatral V., Redkar R.J., Patra G., Mujer C., Los T., Ivanova N., Anderson I., Bhattacharyya A., Lykidis A., Reznik G., Jablonski L., Larsen N., D'Souza M., Bernal A., Mazur M., Goltsman E., Selkov E. , Elzer P.H., Hagius S., O'Callaghan D., Letesson J.-J., Haselkorn R., Kyrpides N.C., Overbeek R. Proc. Natl. Acad. Sci. U.S.A. 99:443-448(2002) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: 16M / ATCC 23456 / NCTC 10094.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AE008918 Genomic DNA. Translation: AAL53945.1.
PIR	AF3597.
RefSeq	NP_541681.1. NC_003318.1.
3D structure databases
ProteinModelPortal	Q8YC29.
ModBase	Search...
Protein-protein interaction databases
STRING	224914.BMEII0703.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblBacteria	AAL53945; AAL53945; BMEII0703.
GeneID	1198475.
KEGG	bme:BMEII0703.
PATRIC	17799660. VBIBruMel146950_2167.
Organism-specific databases
CMR	Search...
Phylogenomic databases
HOGENOM	HOG000224766.
KO	K14441.
OMA	RVDTEHM.
ProtClustDB	PRK14862.
Family and domain databases
Gene3D	2.40.50.140. 1 hit. 3.80.30.20. 1 hit.
HAMAP	MF_01865. MTTase_RimO.
InterPro	IPR006638. Elp3/MiaB/NifB. IPR023970. MeThioTfrase/rSAM. IPR005839. Methylthiotransferase. IPR020612. Methylthiotransferase_CS. IPR013848. Methylthiotransferase_N. IPR012340. NA-bd_OB-fold. IPR005840. Ribosomal_S12_MeSTrfase_RimO. IPR007197. rSAM. IPR023404. rSAM_horseshoe. IPR002792. TRAM_dom. [Graphical view]
PANTHER	PTHR11918. PTHR11918. 1 hit.
Pfam	PF04055. Radical_SAM. 1 hit. PF00919. UPF0004. 1 hit. [Graphical view]
SMART	SM00729. Elp3. 1 hit. [Graphical view]
TIGRFAMs	TIGR00089. TIGR00089. 1 hit. TIGR01125. TIGR01125. 1 hit.
PROSITE	PS51449. MTTASE_N. 1 hit. PS01278. MTTASE_RADICAL. 1 hit. PS50926. TRAM. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

RIMO_BRUME

Accession

Primary (citable) accession number: Q8YC29

Entry history

Integrated into UniProtKB/Swiss-Prot:	May 26, 2009
Last sequence update:	March 1, 2002
Last modified:	May 1, 2013
This is version 80 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program