Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Protein

Ribosomal protein S12 methylthiotransferase RimO

Gene

rimO

Organism
Brucella melitensis biotype 1 (strain 16M / ATCC 23456 / NCTC 10094)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Protein inferred from homologyi

Functioni

Catalyzes the methylthiolation of an aspartic acid residue of ribosomal protein S12.UniRule annotation

Catalytic activityi

L-aspartate-[ribosomal protein S12] + sulfur-(sulfur carrier) + 2 S-adenosyl-L-methionine = 3-methylthio-L-aspartate-[ribosomal protein S12] + S-adenosyl-L-homocysteine + (sulfur carrier) + L-methionine + 5'-deoxyadenosine.UniRule annotation

Cofactori

[4Fe-4S] clusterUniRule annotationNote: Binds 2 [4Fe-4S] clusters. One cluster is coordinated with 3 cysteines and an exchangeable S-adenosyl-L-methionine.UniRule annotation

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Metal bindingi13 – 131Iron-sulfur (4Fe-4S)UniRule annotation
Metal bindingi49 – 491Iron-sulfur (4Fe-4S)UniRule annotation
Metal bindingi78 – 781Iron-sulfur (4Fe-4S)UniRule annotation
Metal bindingi145 – 1451Iron-sulfur (4Fe-4S-S-AdoMet)UniRule annotation
Metal bindingi149 – 1491Iron-sulfur (4Fe-4S-S-AdoMet)UniRule annotation
Metal bindingi152 – 1521Iron-sulfur (4Fe-4S-S-AdoMet)UniRule annotation

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Transferase

Keywords - Ligandi

4Fe-4S, Iron, Iron-sulfur, Metal-binding, S-adenosyl-L-methionine

Names & Taxonomyi

Protein namesi
Recommended name:
Ribosomal protein S12 methylthiotransferase RimOUniRule annotation (EC:2.8.4.4UniRule annotation)
Short name:
S12 MTTaseUniRule annotation
Short name:
S12 methylthiotransferaseUniRule annotation
Alternative name(s):
Ribosomal protein S12 (aspartate-C(3))-methylthiotransferaseUniRule annotation
Ribosome maturation factor RimOUniRule annotation
Gene namesi
Name:rimOUniRule annotation
Ordered Locus Names:BMEII0703
OrganismiBrucella melitensis biotype 1 (strain 16M / ATCC 23456 / NCTC 10094)
Taxonomic identifieri224914 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaAlphaproteobacteriaRhizobialesBrucellaceaeBrucella
ProteomesiUP000000419 Componenti: Chromosome II

Subcellular locationi

  • Cytoplasm UniRule annotation

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 437437Ribosomal protein S12 methylthiotransferase RimOPRO_0000374724Add
BLAST

Interactioni

Protein-protein interaction databases

STRINGi224914.BAWG_1872.

Structurei

3D structure databases

ProteinModelPortaliQ8YC29.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini4 – 114111MTTase N-terminalUniRule annotationAdd
BLAST
Domaini372 – 43766TRAMUniRule annotationAdd
BLAST

Sequence similaritiesi

Belongs to the methylthiotransferase family. RimO subfamily.UniRule annotation
Contains 1 MTTase N-terminal domain.UniRule annotation
Contains 1 TRAM domain.UniRule annotation

Phylogenomic databases

HOGENOMiHOG000224766.
KOiK14441.
OMAiCAIPSFK.
OrthoDBiEOG6P5ZD8.

Family and domain databases

Gene3Di2.40.50.140. 1 hit.
3.80.30.20. 1 hit.
HAMAPiMF_01865. MTTase_RimO.
InterProiIPR006638. Elp3/MiaB/NifB.
IPR023970. MeThioTfrase/rSAM.
IPR005839. Methylthiotransferase.
IPR020612. Methylthiotransferase_CS.
IPR013848. Methylthiotransferase_N.
IPR012340. NA-bd_OB-fold.
IPR005840. Ribosomal_S12_MeSTrfase_RimO.
IPR007197. rSAM.
IPR023404. rSAM_horseshoe.
IPR002792. TRAM_dom.
[Graphical view]
PANTHERiPTHR11918. PTHR11918. 1 hit.
PfamiPF04055. Radical_SAM. 1 hit.
PF00919. UPF0004. 1 hit.
[Graphical view]
SMARTiSM00729. Elp3. 1 hit.
[Graphical view]
TIGRFAMsiTIGR00089. TIGR00089. 1 hit.
TIGR01125. TIGR01125. 1 hit.
PROSITEiPS51449. MTTASE_N. 1 hit.
PS01278. MTTASE_RADICAL. 1 hit.
PS50926. TRAM. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q8YC29-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MSAPRVSFVS LGCPKALVDS ERIITGLRSE GYEISRKHDG ADLVIVNTCG
60 70 80 90 100
FLDSARDESL EAIGLALNEN GKVIVTGCLG AEPDVIRERH PNVLAITGPQ
110 120 130 140 150
AYESVMNAVH EVAPPAHDPF VDLVPPQGVK LTPRHYAYLK ISEGCSNRCS
160 170 180 190 200
FCIIPALRGD LVSRPINEVL REAEKLVQAG VKEILVISQD TSAYGLDIKY
210 220 230 240 250
QEAMWQDRTV RTKFLDLSRE LGEMGVWVRM HYVYPYPHVD EVIPLMAEGK
260 270 280 290 300
ILPYLDIPFQ HASPAVLKNM RRPAHQEKTS RRIQAWRETC PDLAVRSTFI
310 320 330 340 350
VGYPGETEED FQMLLDWLDE AKIERAGCFK YEAVKGAKAN DLGLEQVPEE
360 370 380 390 400
VKEARWHRFM AKQQQISTNL LKKKVGKRLP VIIDEANGTI GKGRTRYDAP
410 420 430
EIDGSVHISS RRPLRVGDIV TVKIEASDAY DLHGTAV
Length:437
Mass (Da):48,774
Last modified:March 1, 2002 - v1
Checksum:i530B6217DDF24656
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AE008918 Genomic DNA. Translation: AAL53945.1.
PIRiAF3597.
RefSeqiWP_002966068.1. NZ_GG703779.1.

Genome annotation databases

EnsemblBacteriaiAAL53945; AAL53945; BMEII0703.
GeneIDi3828258.
KEGGibme:BMEII0703.
PATRICi17799660. VBIBruMel146950_2167.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AE008918 Genomic DNA. Translation: AAL53945.1.
PIRiAF3597.
RefSeqiWP_002966068.1. NZ_GG703779.1.

3D structure databases

ProteinModelPortaliQ8YC29.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi224914.BAWG_1872.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiAAL53945; AAL53945; BMEII0703.
GeneIDi3828258.
KEGGibme:BMEII0703.
PATRICi17799660. VBIBruMel146950_2167.

Phylogenomic databases

HOGENOMiHOG000224766.
KOiK14441.
OMAiCAIPSFK.
OrthoDBiEOG6P5ZD8.

Family and domain databases

Gene3Di2.40.50.140. 1 hit.
3.80.30.20. 1 hit.
HAMAPiMF_01865. MTTase_RimO.
InterProiIPR006638. Elp3/MiaB/NifB.
IPR023970. MeThioTfrase/rSAM.
IPR005839. Methylthiotransferase.
IPR020612. Methylthiotransferase_CS.
IPR013848. Methylthiotransferase_N.
IPR012340. NA-bd_OB-fold.
IPR005840. Ribosomal_S12_MeSTrfase_RimO.
IPR007197. rSAM.
IPR023404. rSAM_horseshoe.
IPR002792. TRAM_dom.
[Graphical view]
PANTHERiPTHR11918. PTHR11918. 1 hit.
PfamiPF04055. Radical_SAM. 1 hit.
PF00919. UPF0004. 1 hit.
[Graphical view]
SMARTiSM00729. Elp3. 1 hit.
[Graphical view]
TIGRFAMsiTIGR00089. TIGR00089. 1 hit.
TIGR01125. TIGR01125. 1 hit.
PROSITEiPS51449. MTTASE_N. 1 hit.
PS01278. MTTASE_RADICAL. 1 hit.
PS50926. TRAM. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

  1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: 16M / ATCC 23456 / NCTC 10094.

Entry informationi

Entry nameiRIMO_BRUME
AccessioniPrimary (citable) accession number: Q8YC29
Entry historyi
Integrated into UniProtKB/Swiss-Prot: May 26, 2009
Last sequence update: March 1, 2002
Last modified: July 22, 2015
This is version 92 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome

Documents

  1. Brucella melitensis
    Brucella melitensis (strain 16M): entries and gene names
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.