Q8YBV9 (BIOD_BRUME) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Dethiobiotin synthetase
EC=6.3.3.3

Alternative name(s):
Dethiobiotin synthase
DTB synthetase
Short name=DTBS

Gene names

Name:	bioD
Ordered Locus Names:	BMEII0777

Organism

Brucella melitensis [Complete proteome] [HAMAP]

Taxonomic identifier

29459 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Alphaproteobacteria › Rhizobiales › Brucellaceae › Brucella

Protein attributes

Sequence length	212 AA.
Sequence status	Complete.
Protein existence	Inferred from homology.

General annotation (Comments)

Catalytic activity	ATP + 7,8-diaminononanoate + CO2 = ADP + phosphate + dethiobiotin. HAMAP MF_00336
Cofactor	Magnesium By similarity. HAMAP MF_00336
Pathway	Cofactor biosynthesis; biotin biosynthesis; biotin from 7,8-diaminononanoate: step 1/2. HAMAP MF_00336
Sequence similarities	Belongs to the dethiobiotin synthetase family.

Ontologies

Keywords
Biological process	Biotin biosynthesis
Ligand	ATP-binding Magnesium Nucleotide-binding
Molecular function	Ligase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	biotin biosynthetic process Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW dethiobiotin synthase activity Inferred from electronic annotation. Source: EC magnesium ion binding Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 212	212	Dethiobiotin synthetase HAMAP MF_00336		PRO_0000187950
Regions
	Nucleotide binding	9 – 17	9	ATP By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q8YBV9-1 [UniParc]. Last modified March 1, 2002. Version 1. Checksum: 0FDA10388F24EFE1	FASTA	212	23,017
	10 20 30 40 50 60 MNSRLIVTGT DTGIGKTVFS AALCHALGAV YWKPVQSGLE EETDSEIVAR LAQASPQRIL 70 80 90 100 110 120 PEAWRLNTPA SPHLSARLDG VEIRPEEMHI PATSLPLVIE GAGGLLVPLN DKTLFADLFA 130 140 150 160 170 180 IWRIPAILCA RAALGTINHT LLSLEAMRSR DIPVLGVAFI GEANEDTETT IAHLGRVKRL 190 200 210 GRLPLLDDLS PEKLHHSFAR NFHIDDFAGV AR

References

[1]

"The genome sequence of the facultative intracellular pathogen Brucella melitensis." DelVecchio V.G., Kapatral V., Redkar R.J., Patra G., Mujer C., Los T., Ivanova N., Anderson I., Bhattacharyya A., Lykidis A., Reznik G., Jablonski L., Larsen N., D'Souza M., Bernal A., Mazur M., Goltsman E., Selkov E. , Elzer P.H., Hagius S., O'Callaghan D., Letesson J.-J., Haselkorn R., Kyrpides N.C., Overbeek R. Proc. Natl. Acad. Sci. U.S.A. 99:443-448(2002) [PubMed: 11756688] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: 16M / ATCC 23456 / NCTC 10094 / Biotype 1.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AE008918 Genomic DNA. Translation: AAL54019.1.
PIR	AH3606.
RefSeq	NP_541755.1.
3D structure databases
ProteinModelPortal	Q8YBV9.
SMR	Q8YBV9. Positions 4-189.
ModBase	Search...
Genome annotation databases
GeneID	1198549.
GenomeReviews	Gene locus BMEII0777 in contig AE008918_GR.
KEGG	bme:BMEII0777.
NMPDR	fig|224914.1.peg.2837.
Organism-specific databases
CMR	Search...
Phylogenomic databases
HOGENOM	HBG650065.
OMA	WKPIQSG.
PhylomeDB	Q8YBV9.
ProtClustDB	PRK00090.
Enzyme and pathway databases
BioCyc	BMEL224914:BMEII0777-MONOMER.
BRENDA	6.3.3.3. 277959.
Family and domain databases
HAMAP	MF_00336. BioD. [Tree]
InterPro	IPR004472. BioD_synth. [Graphical view]
PIRSF	PIRSF006755. DTB_synth. 1 hit.
TIGRFAMs	TIGR00347. bioD. 1 hit.
ProtoNet	Search...

Entry information

Entry name

BIOD_BRUME

Accession

Primary (citable) accession number: Q8YBV9

Entry history

Integrated into UniProtKB/Swiss-Prot:	November 7, 2003
Last sequence update:	March 1, 2002
Last modified:	August 10, 2010
This is version 48 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)