Nitrous-oxide reductase precursor

Contribute

Send feedback

Reviewed, UniProtKB/Swiss-Prot Q8YBC6 (NOSZ_BRUME)

Last modified November 25, 2008. Version 48. History...

Clusters with 100%, 90%, 50% identity |

Documents (3) |

Third-party data |

Customize display

text xml rdf/xml gff fasta

Names and origin · Protein attributes · General annotation (Comments) · Ontologies · Sequence annotation (Features) · Sequences · References · Cross-references · Entry information · Relevant documents

Hide | Top

Names and origin

Protein names

Recommended name:
    Nitrous-oxide reductase
    EC=1.7.99.6
Alternative name(s):
    N(2)OR
    N2O reductase

Gene names

Name:	nosZ
Ordered Locus Names:	BMEII0973/BMEII0974

Organism

Brucella melitensis [Complete proteome] [HAMAP]

Taxonomic identifier

29459 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Alphaproteobacteria › Rhizobiales › Brucellaceae › Brucella

Hide | Top

Protein attributes

Sequence length	639 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Inferred from homology.

Hide | Top

General annotation (Comments)

Function	Nitrous-oxide reductase is part of a bacterial respiratory system which is activated under anaerobic conditions in the presence of nitrate or nitrous oxide By similarity.
Catalytic activity	N(2) + H(2)O + acceptor = N(2)O + reduced acceptor.
Cofactor	Binds 2 calcium ions per subunit By similarity. Binds 6 copper ions per subunit. Each subunit contains 2 copper centers; Cu(A) (binuclear) and Cu(Z) (tetranuclear). Cu(Z) is thought to be the site of nitrous oxide reduction By similarity.
Pathway	Nitrogen metabolism; nitrate reduction (denitrification); dinitrogen from nitrate: step 4/4.
Subunit structure	Homodimer By similarity.
Subcellular location	PeriplasmBy similarity.
Post-translational modification	Predicted to be exported by the Tat system. The position of the signal peptide cleavage has not been experimentally proven.
Sequence similarities	Belongs to the nosZ family. In the C-terminal section; belongs to the cytochrome c oxidase subunit 2 family.
Sequence caution	The sequence AAL54215.1 differs from that shown. Reason: Erroneous termination at position 253. Translated as Gln. The sequence AAL54216.1 differs from that shown. Reason: Erroneous termination at position 253. Translated as Gln.

Hide | Top

Ontologies

Keywords
Cellular component	Periplasm
Domain	Signal
Ligand	Calcium Copper Metal-binding
Molecular function	Oxidoreductase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	oxidation reduction Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	membrane Inferred from electronic annotation. Source: InterPro periplasmic space Inferred from electronic annotation. Source: HAMAP
Molecular function	calcium ion binding Inferred from electronic annotation. Source: HAMAP copper ion binding Inferred from electronic annotation. Source: HAMAP cytochrome-c oxidase activity Inferred from electronic annotation. Source: InterPro nitrous-oxide reductase activity Inferred from electronic annotation. Source: HAMAP
Complete GO annotation...

Hide | Top

Sequence annotation (Features)

Feature key

Position(s)

Length

Description

Graphical view

Feature identifier

Molecule processing

Signal peptide

1 – 44

Tat-type signal Potential

Chain

45 – 639

595

Nitrous-oxide reductase

PRO_0000019825

Regions

Region

541 – 639

COX2-like

Sites

Metal binding

136

Copper Z2 By similarity

Metal binding

137

Copper Z3 By similarity

Metal binding

185

Copper Z2 By similarity

Metal binding

262

Calcium 2; via carbonyl oxygen By similarity

Metal binding

265

Calcium 2 By similarity

Metal binding

273

Calcium 2; via carbonyl oxygen By similarity

Metal binding

279

Calcium 2 By similarity

Metal binding

324

Calcium 2 By similarity

Metal binding

326

Copper Z1 By similarity

Metal binding

381

Copper Z1 By similarity

Metal binding

432

Copper Z3 By similarity

Metal binding

453

Calcium 1; via carbonyl oxygen By similarity

Metal binding

468

Calcium 1 By similarity

Metal binding

493

Copper Z4 By similarity

Metal binding

582

Copper A1 By similarity

Metal binding

617

Copper A1 By similarity

Metal binding

617

Copper A2 By similarity

Metal binding

619

Copper A2; via carbonyl oxygen By similarity

Metal binding

621

Copper A1 By similarity

Metal binding

621

Copper A2 By similarity

Metal binding

625

Copper A2 By similarity

Metal binding

628

Copper A1 By similarity

Hide | Top

Sequences

Sequence

Length

Mass (Da)

Tools

Q8YBC6-1 [UniParc].

Last modified January 9, 2007. Version 3.
Checksum: CFF2E492E332B54E
Show »

FASTA

639

71,087

        10         20         30         40         50         60 
MTEETRKSQL SRRQLLGTSA FVAAAGASGL GGALLAGSSE TALAAGAAKG GMSPEVKPGE 

        70         80         90        100        110        120 
LDEYYVFFSS GQCGEVRIVG LPSMRELMRI PVFNRDSATG WGLTNESRKV LTEGLLPQDR 

       130        140        150        160        170        180 
EFLKDRGDIF LNGDLHHPHP SFTDGTYDGR YLYANDKANT RVCRIRLDVM KCDKIIQLPN 

       190        200        210        220        230        240 
QHTVHGLRVQ KYPKTGYVFC NGEDRVPIPN DGSHLNDVKQ YHAIFTAVDG ETMKVAWQVM 

       250        260        270        280        290        300 
VDGNLDNVDC DYQGKYAFST CYNSEEGTTL AEMMSSEQDW IVVFNLKRIE EAVANGDFKE 

       310        320        330        340        350        360 
MNGVPVIDGR HGSKYTRYIP VPNSPHGINT ALDGIHVVAN GKLSPTVTVF DVRKFDDLFD 

       370        380        390        400        410        420 
DKIKPRDAVV AEPELGLGPL HTAYDDKGNA YTTLFIDSQI CKWNIEDAKR AFKGEKVDPI 

       430        440        450        460        470        480 
RQKLDVHYQP GHNHSSMGQT KEVDGKWLIS LNKFSKDRYL NVGPLKPEND QLIDISGDKM 

       490        500        510        520        530        540 
VIVHDGPSFA EPHDATIVHR SKINPVSFWS REDPFFADAV AQAKADGLDL MEANEVVRDG 

       550        560        570        580        590        600 
NKVRVYMTSS APAFGLTEFT VQQDDEVTVY VTNIDEIEDL THGFAIVNYG VNMEVAPQAT 

       610        620        630 
ASVTFKASKP GVWWYYCSWF CHAMHMEMQG RMLVEPKKA

« Hide

Hide | Top

References

[1]

"The genome sequence of the facultative intracellular pathogen Brucella melitensis."
DelVecchio V.G., Kapatral V., Redkar R.J., Patra G., Mujer C., Los T., Ivanova N., Anderson I., Bhattacharyya A., Lykidis A., Reznik G., Jablonski L., Larsen N., D'Souza M., Bernal A., Mazur M., Goltsman E., Selkov E.

Overbeek R.
Proc. Natl. Acad. Sci. U.S.A. 99:443-448(2002) [PubMed: 11756688] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: 16M / ATCC 23456 / NCTC 10094 / Biotype 1.

Hide | Top

Cross-references

Sequence databases
	AE009730 Genomic DNA. Translation: AAL54215.1. Sequence problems. AE009730 Genomic DNA. Translation: AAL54216.1. Sequence problems.
PIR	AD3631. AE3631.
RefSeq	NP_541951.1. NP_541952.1.
3D structure databases
HSSP	HSSP built from PDB template 1FWX based on UniProtKB Q51705.
ModBase	Search...
Genome annotation databases
GeneID	1198745. 1198746.
GenomeReviews	Gene locus nosZ in contig AE008918_GR.
KEGG	bme:BMEII0973. bme:BMEII0974.
Organism-specific databases
CMR	Search...
Phylogenomic databases
HOGENOM	Q8YBC6.
Enzyme and pathway databases
BioCyc	BMEL224914:BMEII0973-MON. BMEL224914:BMEII0974-MON.
Family and domain databases
HAMAP	MF_00716. [Tree]
InterPro	IPR001505. Copper_CuA. IPR002429. COX2_C. IPR008972. Cupredoxin. IPR006311. Tat. IPR015943. WD40/YVTN_repeat-like. [Graphical view]
Gene3D	G3DSA:2.60.40.420. Cupredoxin. 1 hit. G3DSA:2.130.10.10. WD40/YVTN_repeat-like. 1 hit.
Pfam	PF00116. COX2. 1 hit. [Graphical view]
TIGRFAMs	TIGR01409. TAT_signal_seq. 1 hit.
PROSITE	PS00078. COX2. False negative. PS50857. COX2_CUA. 1 hit. PS51318. TAT. 1 hit. [Graphical view]
ProtoNet	Search...

Hide | Top

Entry information

Entry name

NOSZ_BRUME

Accession

Primary (citable) accession number: Q8YBC6
Secondary accession number(s): Q8YBC7

Entry history

Integrated into UniProtKB/Swiss-Prot:	October 3, 2003
Last sequence update:	January 9, 2007
Last modified:	November 25, 2008
This is version 48 of the entry and version 3 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)

Hide | Top

Relevant documents

Brucella melitensis

Brucella melitensis (strain 16M): entries and gene names

PATHWAY comments

Index of metabolic and biosynthesis pathways

SIMILARITY comments

Index of protein domains and families

Names and origin

Protein attributes

General annotation (Comments)

Ontologies

Keywords

Gene Ontology (GO)

Sequence annotation (Features)

Molecule processing

Regions

Sites

Sequences

References

Cross-references

Sequence databases

3D structure databases

Genome annotation databases

Organism-specific databases

Phylogenomic databases

Enzyme and pathway databases

Family and domain databases

Entry information

Relevant documents