Reviewed, UniProtKB/Swiss-Prot Q8YAS7 (PTC_LISMO)

Names and origin

Protein names

Recommended name:
    Putrescine carbamoyltransferase
      Short name=PTCase
      Short name=PTC
    EC=2.1.3.6
Alternative name(s):
    Putrescine transcarbamylase
    Putrescine transcarbamoylase

Gene names

Name:	ptcA
Ordered Locus Names:	lmo0036

Organism

Listeria monocytogenes [Complete proteome] [HAMAP]

Taxonomic identifier

1639 [NCBI]

Taxonomic lineage

Bacteria › Firmicutes › Bacillales › Listeriaceae › Listeria

Protein attributes

Sequence length	341 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Inferred from homology.

General annotation (Comments)

Function	Catalyzes the phosphorolysis of N-carbamoylputrescine to form carbamoyl phosphate and putrescine. Is involved in the degradation pathway of the polyamine agmatine By similarity.
Catalytic activity	Carbamoyl phosphate + putrescine = phosphate + N-carbamoylputrescine. HAMAP MF_02102
Pathway	Amine and polyamine biosynthesis; putrescine biosynthesis via agmatine pathway; putrescine from N-carbamoylputrescine (transferase route): step 1/1. HAMAP MF_02102
Subunit structure	Homotrimer By similarity.
Subcellular location	Cytoplasm Probable.
Sequence similarities	Belongs to the ATCase/OTCase family. PTCase subfamily.

Ontologies

Keywords
Biological process	Polyamine biosynthesis
Cellular component	Cytoplasm
Molecular function	Transferase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	cellular amino acid metabolic process Inferred from electronic annotation. Source: InterPro putrescine biosynthetic process from arginine via N-carbamoylputrescine Inferred from electronic annotation. Source: HAMAP
Cellular component	ornithine carbamoyltransferase complex Inferred from electronic annotation. Source: InterPro
Molecular function	amino acid binding Inferred from electronic annotation. Source: InterPro ornithine carbamoyltransferase activity Inferred from electronic annotation. Source: InterPro putrescine carbamoyltransferase activity Inferred from electronic annotation. Source: HAMAP
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 341	341	Putrescine carbamoyltransferase HAMAP MF_02102		PRO_0000112946
Regions
	Region	54 – 58	5	Carbamoyl phosphate binding By similarity
	Region	271 – 274	4	Putrescine binding By similarity
Sites
	Binding site	105	1	Carbamoyl phosphate By similarity
	Binding site	132	1	Carbamoyl phosphate By similarity
	Site	29	1	Important for structural integrity By similarity
	Site	145	1	Important for structural integrity By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q8YAS7-1 [UniParc]. Last modified March 1, 2002. Version 1. Checksum: 266C2B25ABD24E6B	FASTA	341	38,428
	10 20 30 40 50 60 MNKKRDFIDT KDFSKEEILF MIEIGRKMKE SIKNGHYPQL LKHKTLGMIF EQSSTRTRVS 70 80 90 100 110 120 FETAMTQLGG HAQYLAPGQI QLGGHESVGD TAKVLSRLVD ILMARVERHQ TVVELANTAA 130 140 150 160 170 180 IPVINGMSDY NHPTQELGDA ITMFEHLPKG KKIEDCKIVF VGDATQVCAS TMFMATKLGM 190 200 210 220 230 240 DFVQFGPKGF QLREEHLKVA EENCEVSGGS YLITEDVDIA LKDADFIYTD VWYGLYEAEL 250 260 270 280 290 300 SEEERMKTFY PKYQVNKELI SKAAPHVKFM HCLPATRGEE VTDEVLDAPY SVVIDEAENR 310 320 330 340 LTAMRALLVY FMNPYVKEAG FAVAEKYDAE LELLLRNGAG L

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Comparative genomics of Listeria species." Glaser P., Frangeul L., Buchrieser C., Rusniok C., Amend A., Baquero F., Berche P., Bloecker H., Brandt P., Chakraborty T., Charbit A., Chetouani F., Couve E., de Daruvar A., Dehoux P., Domann E., Dominguez-Bernal G., Duchaud E. , Durant L., Dussurget O., Entian K.-D., Fsihi H., Garcia-del Portillo F., Garrido P., Gautier L., Goebel W., Gomez-Lopez N., Hain T., Hauf J., Jackson D., Jones L.-M., Kaerst U., Kreft J., Kuhn M., Kunst F., Kurapkat G., Madueno E., Maitournam A., Mata Vicente J., Ng E., Nedjari H., Nordsiek G., Novella S., de Pablos B., Perez-Diaz J.-C., Purcell R., Remmel B., Rose M., Schlueter T., Simoes N., Tierrez A., Vazquez-Boland J.-A., Voss H., Wehland J., Cossart P. Science 294:849-852(2001) [PubMed: 11679669] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC BAA-679 / EGD-e / Serovar 1/2a.
[2]	"Retrieving sequences of enzymes experimentally characterized but erroneously annotated: the case of the putrescine carbamoyltransferase." Naumoff D.G., Xu Y., Glansdorff N., Labedan B. BMC Genomics 5:52-52(2004) [PubMed: 15287962] [Abstract] Cited for: REANNOTATION AS A PTCASE.
[3]	"The difficulty of annotating genes: the case of putrescine carbamoyltransferase." Naumoff D.G., Xu Y., Stalon V., Glansdorff N., Labedan B. Microbiology 150:3908-3911(2004) [PubMed: 15583144] [Abstract] Cited for: GENE NAME.

Cross-references

Sequence databases
EMBLGenBankDDBJ	AL591973 Genomic DNA. Translation: CAC98251.1.
PIR	AE1079.
RefSeq	NP_463569.1.
3D structure databases
HSSP	HSSP built from PDB template 1A1S based on UniProtKB Q51742.
ModBase	Search...
Genome annotation databases
GeneID	985152.
GenomeReviews	Gene locus lmo0036 in contig AL591824_GR.
KEGG	lmo:lmo0036.
Organism-specific databases
ListiList	LMO00036.
CMR	Search...
Phylogenomic databases
HOGENOM	Q8YAS7.
OMA	LGGHAQY.
Enzyme and pathway databases
BioCyc	LMON169963:LMO0036-MON.
BRENDA	2.1.3.3. 96770.
Family and domain databases
HAMAP	MF_02102. [Tree]
InterPro	IPR006132. Asp/Orn_carbamoyltranf_P_bd. IPR006130. Asp/Orn_carbamoylTrfase. IPR006131. Asp_carbamoyltransf_Asp/Orn_bd. IPR002292. Orn_carbamltrans. [Graphical view]
Pfam	PF00185. OTCace. 1 hit. PF02729. OTCace_N. 1 hit. [Graphical view]
PRINTS	PR00100. AOTCASE. PR00102. OTCASE.
TIGRFAMs	TIGR00658. orni_carb_tr. 1 hit.
PROSITE	PS00097. CARBAMOYLTRANSFERASE. False negative. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

PTC_LISMO

Accession

Primary (citable) accession number: Q8YAS7

Entry history

Integrated into UniProtKB/Swiss-Prot:	July 11, 2003
Last sequence update:	March 1, 2002
Last modified:	November 3, 2009
This is version 48 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)