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Q8YAC7

- TILS_LISMO

UniProt

Q8YAC7 - TILS_LISMO

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Protein

Bifunctional protein TilS/HprT

Gene

tilS/hprT

Organism
Listeria monocytogenes serovar 1/2a (strain ATCC BAA-679 / EGD-e)
Status
Reviewed - Annotation score: 3 out of 5- Protein inferred from homologyi

Functioni

Ligates lysine onto the cytidine present at position 34 of the AUA codon-specific tRNA(Ile) that contains the anticodon CAU, in an ATP-dependent manner. Cytidine is converted to lysidine, thus changing the amino acid specificity of the tRNA from methionine to isoleucine (By similarity).By similarity

Catalytic activityi

IMP + diphosphate = hypoxanthine + 5-phospho-alpha-D-ribose 1-diphosphate.
GMP + diphosphate = guanine + 5-phospho-alpha-D-ribose 1-diphosphate.
(tRNA(Ile2))-cytidine(34) + L-lysine + ATP = (tRNA(Ile2))-lysidine(34) + AMP + diphosphate + H2O.

Cofactori

Binds 2 magnesium ions per subunit. One of the ions does not make direct protein contacts (By similarity).By similarity

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Metal bindingi627 – 6271Magnesium 1By similarity

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Nucleotide bindingi29 – 346ATPSequence Analysis

GO - Molecular functioni

  1. ATP binding Source: UniProtKB-HAMAP
  2. guanine phosphoribosyltransferase activity Source: UniProtKB-EC
  3. hypoxanthine phosphoribosyltransferase activity Source: UniProtKB-EC
  4. ligase activity, forming carbon-nitrogen bonds Source: UniProtKB-HAMAP
  5. metal ion binding Source: UniProtKB-KW

GO - Biological processi

  1. purine ribonucleoside salvage Source: InterPro
  2. tRNA modification Source: UniProtKB-HAMAP
Complete GO annotation...

Keywords - Molecular functioni

Glycosyltransferase, Ligase, Transferase

Keywords - Biological processi

tRNA processing

Keywords - Ligandi

ATP-binding, Magnesium, Metal-binding, Nucleotide-binding

Enzyme and pathway databases

BioCyciLMON169963:LMO0219-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
Bifunctional protein TilS/HprT
Including the following 2 domains:
tRNA(Ile)-lysidine synthase (EC:6.3.4.19)
Alternative name(s):
tRNA(Ile)-2-lysyl-cytidine synthase
tRNA(Ile)-lysidine synthetase
Hypoxanthine-guanine phosphoribosyltransferase (EC:2.4.2.8)
Short name:
HGPRT
Short name:
HGPRTase
Gene namesi
Name:tilS/hprT
Ordered Locus Names:lmo0219
OrganismiListeria monocytogenes serovar 1/2a (strain ATCC BAA-679 / EGD-e)
Taxonomic identifieri169963 [NCBI]
Taxonomic lineageiBacteriaFirmicutesBacilliBacillalesListeriaceaeListeria
ProteomesiUP000000817: Chromosome

Organism-specific databases

GenoListiLMO0219.

Subcellular locationi

Cytoplasm By similarity

GO - Cellular componenti

  1. cytoplasm Source: UniProtKB-HAMAP
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 648648Bifunctional protein TilS/HprTPRO_0000181718Add
BLAST

Interactioni

Protein-protein interaction databases

STRINGi169963.lmo0219.

Structurei

3D structure databases

ProteinModelPortaliQ8YAC7.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domaini

The N-terminal region contains the highly conserved SGGXDS motif, predicted to be a P-loop motif involved in ATP binding.

Sequence similaritiesi

In the N-terminal section; belongs to the tRNA(Ile)-lysidine synthase family.Curated
In the C-terminal section; belongs to the purine/pyrimidine phosphoribosyltransferase family.Curated

Phylogenomic databases

eggNOGiCOG0634.
HOGENOMiHOG000217053.
KOiK15780.
OMAiNDISYPL.
OrthoDBiEOG693GNP.
PhylomeDBiQ8YAC7.

Family and domain databases

Gene3Di3.40.50.2020. 1 hit.
3.40.50.620. 1 hit.
HAMAPiMF_01161. tRNA_Ile_lys_synt.
InterProiIPR005904. Hxn_phspho_trans.
IPR012094. Lysidine-tRNA-synth.
IPR012796. Lysidine-tRNA-synth_C.
IPR012795. Lysidine-tRNA-synth_N.
IPR020825. Phe-tRNA_synthase_B3/B4.
IPR000836. PRibTrfase_dom.
IPR029057. PRTase-like.
IPR014729. Rossmann-like_a/b/a_fold.
IPR011063. tRNA-lysidine/thiocyt_synth.
[Graphical view]
PANTHERiPTHR11807:SF2. PTHR11807:SF2. 1 hit.
PfamiPF01171. ATP_bind_3. 1 hit.
PF00156. Pribosyltran. 1 hit.
PF11734. TilS_C. 1 hit.
[Graphical view]
SMARTiSM00977. TilS_C. 1 hit.
[Graphical view]
SUPFAMiSSF53271. SSF53271. 1 hit.
SSF56037. SSF56037. 1 hit.
TIGRFAMsiTIGR01203. HGPRTase. 1 hit.
TIGR02433. lysidine_TilS_C. 1 hit.
TIGR02432. lysidine_TilS_N. 1 hit.
PROSITEiPS00103. PUR_PYR_PR_TRANSFER. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q8YAC7 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MDDTEKRVHK YIEKHDLIRS DDKLLVAVSG GPDSLALLHF LWNSNLVPKE
60 70 80 90 100
AISVAHLNHQ LRENAAKEQR VVETFCERQG IPFYIEEVDI KSRAQSLQKG
110 120 130 140 150
LEETARIVRY DFFEKVMAEK NINKLVLAHH ADDQIETILM RLVRGSASIG
160 170 180 190 200
WSGIQPKREL KGGQAIRPFL PITKAEIIDY AQKHELAYEI DESNTSQEYT
210 220 230 240 250
RNRYRAQLLP FLKQENPAVY SHFERFSEET SEDFQFLEAL ASDLLKKNLI
260 270 280 290 300
KNGKQTTLLL SSFKNEANPL QRRAIHLLLR YLYNEDASFI TVNHIYQIIQ
310 320 330 340 350
MIQSDNPSSS IDLPNKLIAN RAYDKLHFQF GEREAPSEFY HQLELNDRIE
360 370 380 390 400
LDNKASIRLK LKSSVVQTNG LNGMLLDAEE ITLPLIVRNR VNGDRMTMKG
410 420 430 440 450
QAGSKKLKDI FIDAKIPRQE RDKLPVITDY TGKILWVPGV KKSAYDREFS
460 470 480 490 500
RSKKQYIIRY TRNIGGNESM HNDIQKVLIS EDELQEKIRE LGRELTTEYE
510 520 530 540 550
GRNPLVVGVL KGATPFMTDL LKRVDTYLEM DFMDVSSYGN GTVSSGEVKI
560 570 580 590 600
IKDLNASVEG RDVLVIEDII DSGRTLSYLV DLIKYRKAKS VKLVTLLDKP
610 620 630 640
AGRNVEIEAD YVGFVVPNEF VVGYGLDYAE RYRNLPYIGI LKPEIYSE
Length:648
Mass (Da):74,368
Last modified:March 1, 2002 - v1
Checksum:iDACEA788D7AD5F77
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti5 – 51E → D in AAM74001. 1 PublicationCurated
Sequence conflicti57 – 571L → I in AAM74001. 1 PublicationCurated
Sequence conflicti60 – 601Q → H in AAM74001. 1 PublicationCurated
Sequence conflicti67 – 671K → N in AAM74001. 1 PublicationCurated
Sequence conflicti70 – 701R → N in AAM74001. 1 PublicationCurated
Sequence conflicti74 – 741T → A in AAM74001. 1 PublicationCurated
Sequence conflicti78 – 781R → S in AAM74001. 1 PublicationCurated
Sequence conflicti90 – 901I → V in AAM74001. 1 PublicationCurated
Sequence conflicti95 – 951Q → E in AAM74001. 1 PublicationCurated
Sequence conflicti101 – 1011L → I in AAM74001. 1 PublicationCurated
Sequence conflicti121 – 1211N → K in AAM74001. 1 PublicationCurated
Sequence conflicti126 – 1261V → A in AAM74001. 1 PublicationCurated
Sequence conflicti255 – 2551Q → R in AAM74001. 1 PublicationCurated
Sequence conflicti261 – 2622SS → TN in AAM74001. 1 PublicationCurated
Sequence conflicti318 – 3181I → V in AAM74001. 1 PublicationCurated

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AL591974 Genomic DNA. Translation: CAD00746.1.
AF467001 Genomic DNA. Translation: AAM74001.1.
PIRiAD1102.
RefSeqiNP_463750.1. NC_003210.1.

Genome annotation databases

EnsemblBacteriaiCAD00746; CAD00746; CAD00746.
GeneIDi987055.
KEGGilmo:lmo0219.
PATRICi20309445. VBILisMon69206_0224.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AL591974 Genomic DNA. Translation: CAD00746.1 .
AF467001 Genomic DNA. Translation: AAM74001.1 .
PIRi AD1102.
RefSeqi NP_463750.1. NC_003210.1.

3D structure databases

ProteinModelPortali Q8YAC7.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

STRINGi 169963.lmo0219.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblBacteriai CAD00746 ; CAD00746 ; CAD00746 .
GeneIDi 987055.
KEGGi lmo:lmo0219.
PATRICi 20309445. VBILisMon69206_0224.

Organism-specific databases

GenoListi LMO0219.

Phylogenomic databases

eggNOGi COG0634.
HOGENOMi HOG000217053.
KOi K15780.
OMAi NDISYPL.
OrthoDBi EOG693GNP.
PhylomeDBi Q8YAC7.

Enzyme and pathway databases

BioCyci LMON169963:LMO0219-MONOMER.

Family and domain databases

Gene3Di 3.40.50.2020. 1 hit.
3.40.50.620. 1 hit.
HAMAPi MF_01161. tRNA_Ile_lys_synt.
InterProi IPR005904. Hxn_phspho_trans.
IPR012094. Lysidine-tRNA-synth.
IPR012796. Lysidine-tRNA-synth_C.
IPR012795. Lysidine-tRNA-synth_N.
IPR020825. Phe-tRNA_synthase_B3/B4.
IPR000836. PRibTrfase_dom.
IPR029057. PRTase-like.
IPR014729. Rossmann-like_a/b/a_fold.
IPR011063. tRNA-lysidine/thiocyt_synth.
[Graphical view ]
PANTHERi PTHR11807:SF2. PTHR11807:SF2. 1 hit.
Pfami PF01171. ATP_bind_3. 1 hit.
PF00156. Pribosyltran. 1 hit.
PF11734. TilS_C. 1 hit.
[Graphical view ]
SMARTi SM00977. TilS_C. 1 hit.
[Graphical view ]
SUPFAMi SSF53271. SSF53271. 1 hit.
SSF56037. SSF56037. 1 hit.
TIGRFAMsi TIGR01203. HGPRTase. 1 hit.
TIGR02433. lysidine_TilS_C. 1 hit.
TIGR02432. lysidine_TilS_N. 1 hit.
PROSITEi PS00103. PUR_PYR_PR_TRANSFER. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: ATCC BAA-679 / EGD-e.
  2. Li G., Kathariou S.
    Submitted (JAN-2002) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    Strain: 4b1.

Entry informationi

Entry nameiTILS_LISMO
AccessioniPrimary (citable) accession number: Q8YAC7
Secondary accession number(s): Q8KU03
Entry historyi
Integrated into UniProtKB/Swiss-Prot: March 15, 2005
Last sequence update: March 1, 2002
Last modified: October 29, 2014
This is version 86 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3