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Q8YAC7

- TILS_LISMO

UniProt

Q8YAC7 - TILS_LISMO

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Protein
Bifunctional protein TilS/HprT
Gene
tilS/hprT, lmo0219
Organism
Listeria monocytogenes serovar 1/2a (strain ATCC BAA-679 / EGD-e)
Status
Reviewed - Annotation score: 4 out of 5 - Protein inferred from homologyi

Functioni

Ligates lysine onto the cytidine present at position 34 of the AUA codon-specific tRNA(Ile) that contains the anticodon CAU, in an ATP-dependent manner. Cytidine is converted to lysidine, thus changing the amino acid specificity of the tRNA from methionine to isoleucine By similarity.UniRule annotation

Catalytic activityi

IMP + diphosphate = hypoxanthine + 5-phospho-alpha-D-ribose 1-diphosphate.UniRule annotation
GMP + diphosphate = guanine + 5-phospho-alpha-D-ribose 1-diphosphate.UniRule annotation
(tRNA(Ile2))-cytidine(34) + L-lysine + ATP = (tRNA(Ile2))-lysidine(34) + AMP + diphosphate + H2O.UniRule annotation

Cofactori

Binds 2 magnesium ions per subunit. One of the ions does not make direct protein contacts By similarity.

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Metal bindingi627 – 6271Magnesium 1 By similarity

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Nucleotide bindingi29 – 346ATP Reviewed prediction

GO - Molecular functioni

  1. ATP binding Source: UniProtKB-HAMAP
  2. guanine phosphoribosyltransferase activity Source: UniProtKB-EC
  3. hypoxanthine phosphoribosyltransferase activity Source: UniProtKB-EC
  4. ligase activity, forming carbon-nitrogen bonds Source: UniProtKB-HAMAP
  5. metal ion binding Source: UniProtKB-KW
Complete GO annotation...

GO - Biological processi

  1. purine ribonucleoside salvage Source: InterPro
  2. tRNA modification Source: UniProtKB-HAMAP
Complete GO annotation...

Keywords - Molecular functioni

Glycosyltransferase, Ligase, Transferase

Keywords - Biological processi

tRNA processing

Keywords - Ligandi

ATP-binding, Magnesium, Metal-binding, Nucleotide-binding

Enzyme and pathway databases

BioCyciLMON169963:LMO0219-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
Bifunctional protein TilS/HprT
Including the following 2 domains:
tRNA(Ile)-lysidine synthase (EC:6.3.4.19)
Alternative name(s):
tRNA(Ile)-2-lysyl-cytidine synthase
tRNA(Ile)-lysidine synthetase
Hypoxanthine-guanine phosphoribosyltransferase (EC:2.4.2.8)
Short name:
HGPRT
Short name:
HGPRTase
Gene namesi
Name:tilS/hprT
Ordered Locus Names:lmo0219
OrganismiListeria monocytogenes serovar 1/2a (strain ATCC BAA-679 / EGD-e)
Taxonomic identifieri169963 [NCBI]
Taxonomic lineageiBacteriaFirmicutesBacilliBacillalesListeriaceaeListeria
ProteomesiUP000000817: Chromosome

Organism-specific databases

GenoListiLMO0219.

Subcellular locationi

Cytoplasm By similarity UniRule annotation

GO - Cellular componenti

  1. cytoplasm Source: UniProtKB-SubCell
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 648648Bifunctional protein TilS/HprTUniRule annotation
PRO_0000181718Add
BLAST

Interactioni

Protein-protein interaction databases

STRINGi169963.lmo0219.

Structurei

3D structure databases

ProteinModelPortaliQ8YAC7.

Family & Domainsi

Domaini

The N-terminal region contains the highly conserved SGGXDS motif, predicted to be a P-loop motif involved in ATP binding.UniRule annotation

Sequence similaritiesi

In the N-terminal section; belongs to the tRNA(Ile)-lysidine synthase family.
In the C-terminal section; belongs to the purine/pyrimidine phosphoribosyltransferase family.

Phylogenomic databases

eggNOGiCOG0634.
HOGENOMiHOG000217053.
KOiK15780.
OMAiNDISYPL.
OrthoDBiEOG693GNP.
PhylomeDBiQ8YAC7.

Family and domain databases

Gene3Di3.40.50.2020. 1 hit.
3.40.50.620. 1 hit.
HAMAPiMF_01161. tRNA_Ile_lys_synt.
InterProiIPR005904. Hxn_phspho_trans.
IPR012094. Lysidine-tRNA-synth.
IPR012796. Lysidine-tRNA-synth_C.
IPR012795. Lysidine-tRNA-synth_N.
IPR020825. Phe-tRNA_synthase_B3/B4.
IPR000836. PRibTrfase_dom.
IPR029057. PRTase-like.
IPR014729. Rossmann-like_a/b/a_fold.
IPR011063. tRNA-lysidine/thiocyt_synth.
[Graphical view]
PANTHERiPTHR11807:SF2. PTHR11807:SF2. 1 hit.
PfamiPF01171. ATP_bind_3. 1 hit.
PF00156. Pribosyltran. 1 hit.
PF11734. TilS_C. 1 hit.
[Graphical view]
SMARTiSM00977. TilS_C. 1 hit.
[Graphical view]
SUPFAMiSSF53271. SSF53271. 1 hit.
SSF56037. SSF56037. 1 hit.
TIGRFAMsiTIGR01203. HGPRTase. 1 hit.
TIGR02433. lysidine_TilS_C. 1 hit.
TIGR02432. lysidine_TilS_N. 1 hit.
PROSITEiPS00103. PUR_PYR_PR_TRANSFER. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q8YAC7-1 [UniParc]FASTAAdd to Basket

« Hide

MDDTEKRVHK YIEKHDLIRS DDKLLVAVSG GPDSLALLHF LWNSNLVPKE    50
AISVAHLNHQ LRENAAKEQR VVETFCERQG IPFYIEEVDI KSRAQSLQKG 100
LEETARIVRY DFFEKVMAEK NINKLVLAHH ADDQIETILM RLVRGSASIG 150
WSGIQPKREL KGGQAIRPFL PITKAEIIDY AQKHELAYEI DESNTSQEYT 200
RNRYRAQLLP FLKQENPAVY SHFERFSEET SEDFQFLEAL ASDLLKKNLI 250
KNGKQTTLLL SSFKNEANPL QRRAIHLLLR YLYNEDASFI TVNHIYQIIQ 300
MIQSDNPSSS IDLPNKLIAN RAYDKLHFQF GEREAPSEFY HQLELNDRIE 350
LDNKASIRLK LKSSVVQTNG LNGMLLDAEE ITLPLIVRNR VNGDRMTMKG 400
QAGSKKLKDI FIDAKIPRQE RDKLPVITDY TGKILWVPGV KKSAYDREFS 450
RSKKQYIIRY TRNIGGNESM HNDIQKVLIS EDELQEKIRE LGRELTTEYE 500
GRNPLVVGVL KGATPFMTDL LKRVDTYLEM DFMDVSSYGN GTVSSGEVKI 550
IKDLNASVEG RDVLVIEDII DSGRTLSYLV DLIKYRKAKS VKLVTLLDKP 600
AGRNVEIEAD YVGFVVPNEF VVGYGLDYAE RYRNLPYIGI LKPEIYSE 648
Length:648
Mass (Da):74,368
Last modified:March 1, 2002 - v1
Checksum:iDACEA788D7AD5F77
GO

Sequence conflict

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti5 – 51E → D in AAM74001. 1 Publication
Sequence conflicti57 – 571L → I in AAM74001. 1 Publication
Sequence conflicti60 – 601Q → H in AAM74001. 1 Publication
Sequence conflicti67 – 671K → N in AAM74001. 1 Publication
Sequence conflicti70 – 701R → N in AAM74001. 1 Publication
Sequence conflicti74 – 741T → A in AAM74001. 1 Publication
Sequence conflicti78 – 781R → S in AAM74001. 1 Publication
Sequence conflicti90 – 901I → V in AAM74001. 1 Publication
Sequence conflicti95 – 951Q → E in AAM74001. 1 Publication
Sequence conflicti101 – 1011L → I in AAM74001. 1 Publication
Sequence conflicti121 – 1211N → K in AAM74001. 1 Publication
Sequence conflicti126 – 1261V → A in AAM74001. 1 Publication
Sequence conflicti255 – 2551Q → R in AAM74001. 1 Publication
Sequence conflicti261 – 2622SS → TN in AAM74001. 1 Publication
Sequence conflicti318 – 3181I → V in AAM74001. 1 Publication

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AL591974 Genomic DNA. Translation: CAD00746.1.
AF467001 Genomic DNA. Translation: AAM74001.1.
PIRiAD1102.
RefSeqiNP_463750.1. NC_003210.1.

Genome annotation databases

EnsemblBacteriaiCAD00746; CAD00746; CAD00746.
GeneIDi987055.
KEGGilmo:lmo0219.
PATRICi20309445. VBILisMon69206_0224.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AL591974 Genomic DNA. Translation: CAD00746.1 .
AF467001 Genomic DNA. Translation: AAM74001.1 .
PIRi AD1102.
RefSeqi NP_463750.1. NC_003210.1.

3D structure databases

ProteinModelPortali Q8YAC7.
ModBasei Search...

Protein-protein interaction databases

STRINGi 169963.lmo0219.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblBacteriai CAD00746 ; CAD00746 ; CAD00746 .
GeneIDi 987055.
KEGGi lmo:lmo0219.
PATRICi 20309445. VBILisMon69206_0224.

Organism-specific databases

GenoListi LMO0219.

Phylogenomic databases

eggNOGi COG0634.
HOGENOMi HOG000217053.
KOi K15780.
OMAi NDISYPL.
OrthoDBi EOG693GNP.
PhylomeDBi Q8YAC7.

Enzyme and pathway databases

BioCyci LMON169963:LMO0219-MONOMER.

Family and domain databases

Gene3Di 3.40.50.2020. 1 hit.
3.40.50.620. 1 hit.
HAMAPi MF_01161. tRNA_Ile_lys_synt.
InterProi IPR005904. Hxn_phspho_trans.
IPR012094. Lysidine-tRNA-synth.
IPR012796. Lysidine-tRNA-synth_C.
IPR012795. Lysidine-tRNA-synth_N.
IPR020825. Phe-tRNA_synthase_B3/B4.
IPR000836. PRibTrfase_dom.
IPR029057. PRTase-like.
IPR014729. Rossmann-like_a/b/a_fold.
IPR011063. tRNA-lysidine/thiocyt_synth.
[Graphical view ]
PANTHERi PTHR11807:SF2. PTHR11807:SF2. 1 hit.
Pfami PF01171. ATP_bind_3. 1 hit.
PF00156. Pribosyltran. 1 hit.
PF11734. TilS_C. 1 hit.
[Graphical view ]
SMARTi SM00977. TilS_C. 1 hit.
[Graphical view ]
SUPFAMi SSF53271. SSF53271. 1 hit.
SSF56037. SSF56037. 1 hit.
TIGRFAMsi TIGR01203. HGPRTase. 1 hit.
TIGR02433. lysidine_TilS_C. 1 hit.
TIGR02432. lysidine_TilS_N. 1 hit.
PROSITEi PS00103. PUR_PYR_PR_TRANSFER. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: ATCC BAA-679 / EGD-e.
  2. Li G., Kathariou S.
    Submitted (JAN-2002) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    Strain: 4b1.

Entry informationi

Entry nameiTILS_LISMO
AccessioniPrimary (citable) accession number: Q8YAC7
Secondary accession number(s): Q8KU03
Entry historyi
Integrated into UniProtKB/Swiss-Prot: March 15, 2005
Last sequence update: March 1, 2002
Last modified: July 9, 2014
This is version 84 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

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