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Protein

Bifunctional protein TilS/HprT

Gene

tilS/hprT

Organism
Listeria monocytogenes serovar 1/2a (strain ATCC BAA-679 / EGD-e)
Status
Reviewed-Annotation score: -Protein inferred from homologyi

Functioni

Ligates lysine onto the cytidine present at position 34 of the AUA codon-specific tRNA(Ile) that contains the anticodon CAU, in an ATP-dependent manner. Cytidine is converted to lysidine, thus changing the amino acid specificity of the tRNA from methionine to isoleucine (By similarity).By similarity

Catalytic activityi

IMP + diphosphate = hypoxanthine + 5-phospho-alpha-D-ribose 1-diphosphate.
GMP + diphosphate = guanine + 5-phospho-alpha-D-ribose 1-diphosphate.
(tRNA(Ile2))-cytidine(34) + L-lysine + ATP = (tRNA(Ile2))-lysidine(34) + AMP + diphosphate + H2O.

Cofactori

Mg2+By similarityNote: Binds 2 magnesium ions per subunit. One of the ions does not make direct protein contacts.By similarity

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Metal bindingi627Magnesium 1By similarity1

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi29 – 34ATPSequence analysis6

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionGlycosyltransferase, Ligase, Transferase
Biological processtRNA processing
LigandATP-binding, Magnesium, Metal-binding, Nucleotide-binding

Enzyme and pathway databases

BioCyciLMON169963:G1GTA-225-MONOMER

Names & Taxonomyi

Protein namesi
Recommended name:
Bifunctional protein TilS/HprT
Including the following 2 domains:
tRNA(Ile)-lysidine synthase (EC:6.3.4.19)
Alternative name(s):
tRNA(Ile)-2-lysyl-cytidine synthase
tRNA(Ile)-lysidine synthetase
Hypoxanthine-guanine phosphoribosyltransferase (EC:2.4.2.8)
Short name:
HGPRT
Short name:
HGPRTase
Gene namesi
Name:tilS/hprT
Ordered Locus Names:lmo0219
OrganismiListeria monocytogenes serovar 1/2a (strain ATCC BAA-679 / EGD-e)
Taxonomic identifieri169963 [NCBI]
Taxonomic lineageiBacteriaFirmicutesBacilliBacillalesListeriaceaeListeria
Proteomesi
  • UP000000817 Componenti: Chromosome

Subcellular locationi

GO - Cellular componenti

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001817181 – 648Bifunctional protein TilS/HprTAdd BLAST648

Proteomic databases

PaxDbiQ8YAC7
PRIDEiQ8YAC7

Interactioni

Protein-protein interaction databases

STRINGi169963.lmo0219

Structurei

3D structure databases

ProteinModelPortaliQ8YAC7
SMRiQ8YAC7
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domaini

The N-terminal region contains the highly conserved SGGXDS motif, predicted to be a P-loop motif involved in ATP binding.

Sequence similaritiesi

In the N-terminal section; belongs to the tRNA(Ile)-lysidine synthase family.Curated
In the C-terminal section; belongs to the purine/pyrimidine phosphoribosyltransferase family.Curated

Phylogenomic databases

eggNOGiENOG4105D3U Bacteria
COG0037 LUCA
COG0634 LUCA
HOGENOMiHOG000217053
KOiK15780
OMAiAHCNFNL
PhylomeDBiQ8YAC7

Family and domain databases

CDDicd01992 PP-ATPase, 1 hit
cd06223 PRTases_typeI, 1 hit
Gene3Di3.40.50.620, 1 hit
HAMAPiMF_01161 tRNA_Ile_lys_synt, 1 hit
InterProiView protein in InterPro
IPR005904 Hxn_phspho_trans
IPR012796 Lysidine-tRNA-synth_C
IPR000836 PRibTrfase_dom
IPR029057 PRTase-like
IPR014729 Rossmann-like_a/b/a_fold
IPR011063 TilS/TtcA_N
IPR012094 tRNA_Ile_lys_synt
IPR012795 tRNA_Ile_lys_synt_N
PANTHERiPTHR43033:SF1 PTHR43033:SF1, 1 hit
PfamiView protein in Pfam
PF01171 ATP_bind_3, 1 hit
PF00156 Pribosyltran, 1 hit
PF11734 TilS_C, 1 hit
SMARTiView protein in SMART
SM00977 TilS_C, 1 hit
SUPFAMiSSF53271 SSF53271, 1 hit
TIGRFAMsiTIGR01203 HGPRTase, 1 hit
TIGR02433 lysidine_TilS_C, 1 hit
TIGR02432 lysidine_TilS_N, 1 hit
PROSITEiView protein in PROSITE
PS00103 PUR_PYR_PR_TRANSFER, 1 hit

Sequencei

Sequence statusi: Complete.

Q8YAC7-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MDDTEKRVHK YIEKHDLIRS DDKLLVAVSG GPDSLALLHF LWNSNLVPKE
60 70 80 90 100
AISVAHLNHQ LRENAAKEQR VVETFCERQG IPFYIEEVDI KSRAQSLQKG
110 120 130 140 150
LEETARIVRY DFFEKVMAEK NINKLVLAHH ADDQIETILM RLVRGSASIG
160 170 180 190 200
WSGIQPKREL KGGQAIRPFL PITKAEIIDY AQKHELAYEI DESNTSQEYT
210 220 230 240 250
RNRYRAQLLP FLKQENPAVY SHFERFSEET SEDFQFLEAL ASDLLKKNLI
260 270 280 290 300
KNGKQTTLLL SSFKNEANPL QRRAIHLLLR YLYNEDASFI TVNHIYQIIQ
310 320 330 340 350
MIQSDNPSSS IDLPNKLIAN RAYDKLHFQF GEREAPSEFY HQLELNDRIE
360 370 380 390 400
LDNKASIRLK LKSSVVQTNG LNGMLLDAEE ITLPLIVRNR VNGDRMTMKG
410 420 430 440 450
QAGSKKLKDI FIDAKIPRQE RDKLPVITDY TGKILWVPGV KKSAYDREFS
460 470 480 490 500
RSKKQYIIRY TRNIGGNESM HNDIQKVLIS EDELQEKIRE LGRELTTEYE
510 520 530 540 550
GRNPLVVGVL KGATPFMTDL LKRVDTYLEM DFMDVSSYGN GTVSSGEVKI
560 570 580 590 600
IKDLNASVEG RDVLVIEDII DSGRTLSYLV DLIKYRKAKS VKLVTLLDKP
610 620 630 640
AGRNVEIEAD YVGFVVPNEF VVGYGLDYAE RYRNLPYIGI LKPEIYSE
Length:648
Mass (Da):74,368
Last modified:March 1, 2002 - v1
Checksum:iDACEA788D7AD5F77
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti5E → D in AAM74001 (Ref. 2) Curated1
Sequence conflicti57L → I in AAM74001 (Ref. 2) Curated1
Sequence conflicti60Q → H in AAM74001 (Ref. 2) Curated1
Sequence conflicti67K → N in AAM74001 (Ref. 2) Curated1
Sequence conflicti70R → N in AAM74001 (Ref. 2) Curated1
Sequence conflicti74T → A in AAM74001 (Ref. 2) Curated1
Sequence conflicti78R → S in AAM74001 (Ref. 2) Curated1
Sequence conflicti90I → V in AAM74001 (Ref. 2) Curated1
Sequence conflicti95Q → E in AAM74001 (Ref. 2) Curated1
Sequence conflicti101L → I in AAM74001 (Ref. 2) Curated1
Sequence conflicti121N → K in AAM74001 (Ref. 2) Curated1
Sequence conflicti126V → A in AAM74001 (Ref. 2) Curated1
Sequence conflicti255Q → R in AAM74001 (Ref. 2) Curated1
Sequence conflicti261 – 262SS → TN in AAM74001 (Ref. 2) Curated2
Sequence conflicti318I → V in AAM74001 (Ref. 2) Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AL591974 Genomic DNA Translation: CAD00746.1
AF467001 Genomic DNA Translation: AAM74001.1
PIRiAD1102
RefSeqiNP_463750.1, NC_003210.1
WP_003723741.1, NC_003210.1

Genome annotation databases

EnsemblBacteriaiCAD00746; CAD00746; CAD00746
GeneIDi987055
KEGGilmo:lmo0219
PATRICifig|169963.11.peg.224

Similar proteinsi

Entry informationi

Entry nameiTILS_LISMO
AccessioniPrimary (citable) accession number: Q8YAC7
Secondary accession number(s): Q8KU03
Entry historyiIntegrated into UniProtKB/Swiss-Prot: March 15, 2005
Last sequence update: March 1, 2002
Last modified: May 23, 2018
This is version 103 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

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