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Protein

2-C-methyl-D-erythritol 4-phosphate cytidylyltransferase 1

Gene

ispD1

Organism
Listeria monocytogenes serovar 1/2a (strain ATCC BAA-679 / EGD-e)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Protein inferred from homologyi

Functioni

Catalyzes the formation of 4-diphosphocytidyl-2-C-methyl-D-erythritol from CTP and 2-C-methyl-D-erythritol 4-phosphate (MEP).By similarity

Catalytic activityi

CTP + 2-C-methyl-D-erythritol 4-phosphate = diphosphate + 4-(cytidine 5'-diphospho)-2-C-methyl-D-erythritol.

Pathwayi: isopentenyl diphosphate biosynthesis via DXP pathway

This protein is involved in step 2 of the subpathway that synthesizes isopentenyl diphosphate from 1-deoxy-D-xylulose 5-phosphate.
Proteins known to be involved in the 6 steps of the subpathway in this organism are:
  1. 1-deoxy-D-xylulose 5-phosphate reductoisomerase (dxr)
  2. Putative 2-C-methyl-D-erythritol 4-phosphate cytidylyltransferase 2 (ispD2), 2-C-methyl-D-erythritol 4-phosphate cytidylyltransferase 1 (ispD1)
  3. 4-diphosphocytidyl-2-C-methyl-D-erythritol kinase (ispE)
  4. 2-C-methyl-D-erythritol 2,4-cyclodiphosphate synthase (ispF)
  5. 4-hydroxy-3-methylbut-2-en-1-yl diphosphate synthase (flavodoxin) (ispG)
  6. 4-hydroxy-3-methylbut-2-enyl diphosphate reductase (ispH)
This subpathway is part of the pathway isopentenyl diphosphate biosynthesis via DXP pathway, which is itself part of Isoprenoid biosynthesis.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes isopentenyl diphosphate from 1-deoxy-D-xylulose 5-phosphate, the pathway isopentenyl diphosphate biosynthesis via DXP pathway and in Isoprenoid biosynthesis.

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sitei15 – 151Transition state stabilizerBy similarity
Sitei22 – 221Transition state stabilizerBy similarity
Sitei153 – 1531Positions MEP for the nucleophilic attackBy similarity
Sitei209 – 2091Positions MEP for the nucleophilic attackBy similarity

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Nucleotidyltransferase, Transferase

Keywords - Biological processi

Isoprene biosynthesis

Enzyme and pathway databases

BioCyciLMON169963:LMO0235-MONOMER.
UniPathwayiUPA00056; UER00093.

Names & Taxonomyi

Protein namesi
Recommended name:
2-C-methyl-D-erythritol 4-phosphate cytidylyltransferase 1 (EC:2.7.7.60)
Alternative name(s):
4-diphosphocytidyl-2C-methyl-D-erythritol synthase 1
MEP cytidylyltransferase 1
Short name:
MCT 1
Gene namesi
Name:ispD1
Synonyms:ispD
Ordered Locus Names:lmo0235
OrganismiListeria monocytogenes serovar 1/2a (strain ATCC BAA-679 / EGD-e)
Taxonomic identifieri169963 [NCBI]
Taxonomic lineageiBacteriaFirmicutesBacilliBacillalesListeriaceaeListeria
Proteomesi
  • UP000000817 Componenti: Chromosome

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 2322322-C-methyl-D-erythritol 4-phosphate cytidylyltransferase 1PRO_0000075587Add
BLAST

Proteomic databases

PaxDbiQ8YAB5.

Interactioni

Protein-protein interaction databases

STRINGi169963.lmo0235.

Structurei

3D structure databases

ProteinModelPortaliQ8YAB5.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the IspD family.Curated

Phylogenomic databases

eggNOGiENOG4105CE5. Bacteria.
COG1211. LUCA.
HOGENOMiHOG000218563.
KOiK00991.
OMAiDYGGKER.
PhylomeDBiQ8YAB5.

Family and domain databases

Gene3Di3.90.550.10. 1 hit.
HAMAPiMF_00108. IspD. 1 hit.
InterProiIPR001228. IspD.
IPR018294. ISPD_synthase_CS.
IPR029044. Nucleotide-diphossugar_trans.
[Graphical view]
PfamiPF01128. IspD. 1 hit.
[Graphical view]
SUPFAMiSSF53448. SSF53448. 1 hit.
TIGRFAMsiTIGR00453. ispD. 1 hit.
PROSITEiPS01295. ISPD. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q8YAB5-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MNYELVFLAA GQGKRMNAQK NKMWLELVGE PIFIHALRPF LADNRCSKVI
60 70 80 90 100
VVCQEEERKH VKELMSQLNV AEHRIEIVKG GSERQYSVAA GLERCGTGRV
110 120 130 140 150
VLVHDGARPF ITLDIIDRLL IGVEQSKAAI CAVKVKDTVK RVMNGVVQET
160 170 180 190 200
VDRENLWQVQ TPQAFELPIL RKAHQLARKE QFLGTDEASL VERIPCPVAI
210 220 230
VQGSYYNIKL TTPEDMPLAK AILGELGGIA ND
Length:232
Mass (Da):25,910
Last modified:March 5, 2002 - v1
Checksum:iD8DEDEC6B391E969
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AL591974 Genomic DNA. Translation: CAD00762.1.
PIRiAD1104.
RefSeqiNP_463766.1. NC_003210.1.
WP_010989381.1. NC_003210.1.

Genome annotation databases

EnsemblBacteriaiCAD00762; CAD00762; CAD00762.
GeneIDi987220.
KEGGilmo:lmo0235.
PATRICi20309514. VBILisMon69206_0243.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AL591974 Genomic DNA. Translation: CAD00762.1.
PIRiAD1104.
RefSeqiNP_463766.1. NC_003210.1.
WP_010989381.1. NC_003210.1.

3D structure databases

ProteinModelPortaliQ8YAB5.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi169963.lmo0235.

Proteomic databases

PaxDbiQ8YAB5.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiCAD00762; CAD00762; CAD00762.
GeneIDi987220.
KEGGilmo:lmo0235.
PATRICi20309514. VBILisMon69206_0243.

Phylogenomic databases

eggNOGiENOG4105CE5. Bacteria.
COG1211. LUCA.
HOGENOMiHOG000218563.
KOiK00991.
OMAiDYGGKER.
PhylomeDBiQ8YAB5.

Enzyme and pathway databases

UniPathwayiUPA00056; UER00093.
BioCyciLMON169963:LMO0235-MONOMER.

Family and domain databases

Gene3Di3.90.550.10. 1 hit.
HAMAPiMF_00108. IspD. 1 hit.
InterProiIPR001228. IspD.
IPR018294. ISPD_synthase_CS.
IPR029044. Nucleotide-diphossugar_trans.
[Graphical view]
PfamiPF01128. IspD. 1 hit.
[Graphical view]
SUPFAMiSSF53448. SSF53448. 1 hit.
TIGRFAMsiTIGR00453. ispD. 1 hit.
PROSITEiPS01295. ISPD. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiISPD1_LISMO
AccessioniPrimary (citable) accession number: Q8YAB5
Entry historyi
Integrated into UniProtKB/Swiss-Prot: March 5, 2002
Last sequence update: March 5, 2002
Last modified: September 7, 2016
This is version 85 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.