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Protein

Glutamate--tRNA ligase

Gene

gltX

Organism
Listeria monocytogenes serovar 1/2a (strain ATCC BAA-679 / EGD-e)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Protein inferred from homologyi

Functioni

Catalyzes the attachment of glutamate to tRNA(Glu) in a two-step reaction: glutamate is first activated by ATP to form Glu-AMP and then transferred to the acceptor end of tRNA(Glu).UniRule annotation

Catalytic activityi

ATP + L-glutamate + tRNA(Glu) = AMP + diphosphate + L-glutamyl-tRNA(Glu).UniRule annotation

Cofactori

Zn2+UniRule annotationNote: Binds 1 zinc ion per subunit.UniRule annotation

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Metal bindingi110ZincUniRule annotation1
Metal bindingi112ZincUniRule annotation1
Metal bindingi137ZincUniRule annotation1
Metal bindingi139ZincUniRule annotation1
Binding sitei257ATPUniRule annotation1

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionAminoacyl-tRNA synthetase, Ligase
Biological processProtein biosynthesis
LigandATP-binding, Metal-binding, Nucleotide-binding, Zinc

Names & Taxonomyi

Protein namesi
Recommended name:
Glutamate--tRNA ligaseUniRule annotation (EC:6.1.1.17UniRule annotation)
Alternative name(s):
Glutamyl-tRNA synthetaseUniRule annotation
Short name:
GluRSUniRule annotation
Gene namesi
Name:gltXUniRule annotation
Ordered Locus Names:lmo0237
OrganismiListeria monocytogenes serovar 1/2a (strain ATCC BAA-679 / EGD-e)
Taxonomic identifieri169963 [NCBI]
Taxonomic lineageiBacteriaFirmicutesBacilliBacillalesListeriaceaeListeria
Proteomesi
  • UP000000817 Componenti: Chromosome

Subcellular locationi

  • Cytoplasm UniRule annotation

GO - Cellular componenti

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001195941 – 491Glutamate--tRNA ligaseAdd BLAST491

Proteomic databases

PaxDbiQ8YAB3.

Interactioni

Subunit structurei

Monomer.UniRule annotation

Protein-protein interaction databases

STRINGi169963.lmo0237.

Structurei

3D structure databases

ProteinModelPortaliQ8YAB3.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Motif

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Motifi13 – 23"HIGH" regionUniRule annotationAdd BLAST11
Motifi254 – 258"KMSKS" regionUniRule annotation5

Sequence similaritiesi

Belongs to the class-I aminoacyl-tRNA synthetase family. Glutamate--tRNA ligase type 1 subfamily.UniRule annotation

Phylogenomic databases

eggNOGiENOG4105C20. Bacteria.
COG0008. LUCA.
HOGENOMiHOG000252720.
KOiK09698.
OMAiNWINSQY.
PhylomeDBiQ8YAB3.

Family and domain databases

CDDicd00808. GluRS_core. 1 hit.
Gene3Di1.10.10.350. 1 hit.
1.10.1160.10. 1 hit.
3.40.50.620. 2 hits.
HAMAPiMF_00022. Glu_tRNA_synth_type1. 1 hit.
InterProiView protein in InterPro
IPR008925. aa-tRNA-synth_I_codon-bd.
IPR020751. aa-tRNA-synth_I_codon-bd_sub2.
IPR001412. aa-tRNA-synth_I_CS.
IPR004527. Glu-tRNA-ligase_bac/mito.
IPR000924. Glu/Gln-tRNA-synth.
IPR020061. Glu/Gln-tRNA-synth_Ib_a-bdl.
IPR020058. Glu/Gln-tRNA-synth_Ib_cat-dom.
IPR033910. GluRS_core.
IPR014729. Rossmann-like_a/b/a_fold.
PfamiView protein in Pfam
PF00749. tRNA-synt_1c. 1 hit.
PRINTSiPR00987. TRNASYNTHGLU.
SUPFAMiSSF48163. SSF48163. 1 hit.
TIGRFAMsiTIGR00464. gltX_bact. 1 hit.
PROSITEiView protein in PROSITE
PS00178. AA_TRNA_LIGASE_I. 1 hit.

Sequencei

Sequence statusi: Complete.

Q8YAB3-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MSETKRVRVR YAPSPTGFLH IGNARTALFN YLFARHNDGD FIIRIEDTDA
60 70 80 90 100
KRNIADGEES QMTNLKWLGM DWDEGVDVPG KYGPYRQSER QSIYEPLIQE
110 120 130 140 150
LLDKDLAYKC YCTEEELDAE REKQKANGEM PRYSGKCRHL TKEQQAEKEA
160 170 180 190 200
QGFKPSIRFK VPANETITFN DMVKDDVSFE SNGIGDFVIA KKDGIPTYNF
210 220 230 240 250
AVAVDDHLME ISHVLRGDDH ISNTPKQILI YNAFGWEPPI FGHMTLIVNE
260 270 280 290 300
SRRKLSKRDG SIIQFIEQYR DLGYLPEALF NFIAMLGWSP EGEEEIFSKE
310 320 330 340 350
EFIKMFDPKR LSKSPALFDN VKLTWVNNQY VKKLPLNDVV ELSLPHLQKA
360 370 380 390 400
GVVSAELNQA ELDWVHKLVS LYHEQMSYGA EIVPLSEMFF ADAESITFDE
410 420 430 440 450
EETAVLAEET VPTVISAFKK ELEALEVLEA AEVKAAIKRV QKETGVKGKG
460 470 480 490
LFMPIRIVTT GEMHGPELPL AIEVLGREKV LNRLDTWLKN N
Length:491
Mass (Da):56,036
Last modified:March 1, 2002 - v1
Checksum:i7A1744ED14F62FE6
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AL591974 Genomic DNA. Translation: CAD00764.1.
PIRiAF1104.
RefSeqiNP_463768.1. NC_003210.1.
WP_003732826.1. NC_003210.1.

Genome annotation databases

EnsemblBacteriaiCAD00764; CAD00764; CAD00764.
GeneIDi987229.
KEGGilmo:lmo0237.
PATRICi20309518. VBILisMon69206_0245.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AL591974 Genomic DNA. Translation: CAD00764.1.
PIRiAF1104.
RefSeqiNP_463768.1. NC_003210.1.
WP_003732826.1. NC_003210.1.

3D structure databases

ProteinModelPortaliQ8YAB3.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi169963.lmo0237.

Proteomic databases

PaxDbiQ8YAB3.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiCAD00764; CAD00764; CAD00764.
GeneIDi987229.
KEGGilmo:lmo0237.
PATRICi20309518. VBILisMon69206_0245.

Phylogenomic databases

eggNOGiENOG4105C20. Bacteria.
COG0008. LUCA.
HOGENOMiHOG000252720.
KOiK09698.
OMAiNWINSQY.
PhylomeDBiQ8YAB3.

Family and domain databases

CDDicd00808. GluRS_core. 1 hit.
Gene3Di1.10.10.350. 1 hit.
1.10.1160.10. 1 hit.
3.40.50.620. 2 hits.
HAMAPiMF_00022. Glu_tRNA_synth_type1. 1 hit.
InterProiView protein in InterPro
IPR008925. aa-tRNA-synth_I_codon-bd.
IPR020751. aa-tRNA-synth_I_codon-bd_sub2.
IPR001412. aa-tRNA-synth_I_CS.
IPR004527. Glu-tRNA-ligase_bac/mito.
IPR000924. Glu/Gln-tRNA-synth.
IPR020061. Glu/Gln-tRNA-synth_Ib_a-bdl.
IPR020058. Glu/Gln-tRNA-synth_Ib_cat-dom.
IPR033910. GluRS_core.
IPR014729. Rossmann-like_a/b/a_fold.
PfamiView protein in Pfam
PF00749. tRNA-synt_1c. 1 hit.
PRINTSiPR00987. TRNASYNTHGLU.
SUPFAMiSSF48163. SSF48163. 1 hit.
TIGRFAMsiTIGR00464. gltX_bact. 1 hit.
PROSITEiView protein in PROSITE
PS00178. AA_TRNA_LIGASE_I. 1 hit.
ProtoNetiSearch...

Entry informationi

Entry nameiSYE_LISMO
AccessioniPrimary (citable) accession number: Q8YAB3
Entry historyiIntegrated into UniProtKB/Swiss-Prot: August 13, 2002
Last sequence update: March 1, 2002
Last modified: February 15, 2017
This is version 92 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Aminoacyl-tRNA synthetases
    List of aminoacyl-tRNA synthetase entries
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.