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Protein

Shikimate dehydrogenase (NADP(+))

Gene

aroE

Organism
Listeria monocytogenes serovar 1/2a (strain ATCC BAA-679 / EGD-e)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at protein leveli

Functioni

Involved in the biosynthesis of the chorismate, which leads to the biosynthesis of aromatic amino acids. Catalyzes the reversible NADPH linked reduction of 3-dehydroshikimate (DHSA) to yield shikimate (SA).UniRule annotation

Catalytic activityi

Shikimate + NADP+ = 3-dehydroshikimate + NADPH.UniRule annotation

Pathwayi: chorismate biosynthesis

This protein is involved in step 4 of the subpathway that synthesizes chorismate from D-erythrose 4-phosphate and phosphoenolpyruvate.UniRule annotation
Proteins known to be involved in the 7 steps of the subpathway in this organism are:
  1. no protein annotated in this organism
  2. 3-dehydroquinate synthase (aroB)
  3. 3-dehydroquinate dehydratase (aroD)
  4. Shikimate dehydrogenase (NADP(+)) (aroE), Shikimate dehydrogenase (NADP(+)) (aroE), Shikimate dehydrogenase (NADP(+)) (aroE)
  5. Shikimate kinase (aroK)
  6. 3-phosphoshikimate 1-carboxyvinyltransferase (aroA)
  7. Chorismate synthase (aroC)
This subpathway is part of the pathway chorismate biosynthesis, which is itself part of Metabolic intermediate biosynthesis.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes chorismate from D-erythrose 4-phosphate and phosphoenolpyruvate, the pathway chorismate biosynthesis and in Metabolic intermediate biosynthesis.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei73ShikimateUniRule annotation1 Publication1
Active sitei77Proton acceptorUniRule annotation1 Publication1
Binding sitei98ShikimateUniRule annotation1 Publication1
Binding sitei113ShikimateUniRule annotation1 Publication1
Binding sitei214NADP1 Publication1
Binding sitei238NADP; via carbonyl oxygenUniRule annotation1 Publication1
Binding sitei240ShikimateUniRule annotation1
Binding sitei261NADP; via carbonyl oxygenUniRule annotation1 Publication1

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi137 – 141NADPUniRule annotation1 Publication5
Nucleotide bindingi161 – 164NAD1 Publication4

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Oxidoreductase

Keywords - Biological processi

Amino-acid biosynthesis, Aromatic amino acid biosynthesis

Keywords - Ligandi

NADP

Enzyme and pathway databases

UniPathwayiUPA00053; UER00087.

Names & Taxonomyi

Protein namesi
Recommended name:
Shikimate dehydrogenase (NADP(+))UniRule annotation (EC:1.1.1.25UniRule annotation)
Short name:
SDHUniRule annotation
Gene namesi
Name:aroEUniRule annotation
Ordered Locus Names:lmo0490
OrganismiListeria monocytogenes serovar 1/2a (strain ATCC BAA-679 / EGD-e)
Taxonomic identifieri169963 [NCBI]
Taxonomic lineageiBacteriaFirmicutesBacilliBacillalesListeriaceaeListeria
Proteomesi
  • UP000000817 Componenti: Chromosome

Subcellular locationi

GO - Cellular componenti

Complete GO annotation...

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001360141 – 291Shikimate dehydrogenase (NADP(+))Add BLAST291

Proteomic databases

PaxDbiQ8Y9N5.

Interactioni

Subunit structurei

Homodimer or homotetramer.UniRule annotation1 Publication

Protein-protein interaction databases

STRINGi169963.lmo0490.

Structurei

Secondary structure

1291
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Beta strandi14 – 22Combined sources9
Helixi28 – 39Combined sources12
Beta strandi43 – 49Combined sources7
Helixi52 – 64Combined sources13
Beta strandi69 – 72Combined sources4
Turni77 – 79Combined sources3
Helixi80 – 83Combined sources4
Beta strandi85 – 87Combined sources3
Helixi89 – 94Combined sources6
Beta strandi98 – 103Combined sources6
Beta strandi106 – 110Combined sources5
Helixi113 – 123Combined sources11
Beta strandi131 – 136Combined sources6
Helixi140 – 151Combined sources12
Beta strandi155 – 161Combined sources7
Helixi167 – 180Combined sources14
Beta strandi184 – 189Combined sources6
Helixi193 – 201Combined sources9
Beta strandi204 – 208Combined sources5
Helixi226 – 228Combined sources3
Beta strandi234 – 238Combined sources5
Beta strandi241 – 244Combined sources4
Helixi246 – 253Combined sources8
Beta strandi257 – 259Combined sources3
Helixi262 – 277Combined sources16
Helixi283 – 290Combined sources8

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
3TNLX-ray1.45A/B/C/D1-291[»]
3TOZX-ray2.20A/B/C/D/E/F/G/H1-291[»]
ProteinModelPortaliQ8Y9N5.
SMRiQ8Y9N5.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni26 – 28Shikimate bindingUniRule annotation1 Publication3

Sequence similaritiesi

Belongs to the shikimate dehydrogenase family.UniRule annotation

Phylogenomic databases

eggNOGiENOG4105E2X. Bacteria.
COG0169. LUCA.
HOGENOMiHOG000237875.
KOiK00014.
OMAiSIFARND.
PhylomeDBiQ8Y9N5.

Family and domain databases

Gene3Di3.40.50.720. 1 hit.
HAMAPiMF_00222. Shikimate_DH_AroE. 1 hit.
InterProiIPR016040. NAD(P)-bd_dom.
IPR011342. Shikimate_DH.
IPR013708. Shikimate_DH-bd_N.
IPR022893. Shikimate_DH_fam.
[Graphical view]
PfamiPF08501. Shikimate_dh_N. 1 hit.
[Graphical view]
SUPFAMiSSF51735. SSF51735. 1 hit.
TIGRFAMsiTIGR00507. aroE. 1 hit.

Sequencei

Sequence statusi: Complete.

Q8Y9N5-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MTNKITERIT GHTELIGLIA TPIRHSLSPT MHNEAFAKLG LDYVYLAFEV
60 70 80 90 100
GDKELKDVVQ GFRAMNLRGW NVSMPNKTNI HKYLDKLSPA AELVGAVNTV
110 120 130 140 150
VNDDGVLTGH ITDGTGYMRA LKEAGHDIIG KKMTICGAGG AATAICIQAA
160 170 180 190 200
LDGVKEISIF NRKDDFYANA EKTVEKINSK TDCKAQLFDI EDHEQLRKEI
210 220 230 240 250
AESVIFTNAT GVGMKPFEGE TLLPSADMLR PELIVSDVVY KPTKTRLLEI
260 270 280 290
AEEQGCQTLN GLGMMLWQGA KAFEIWTHKE MPVDYIKEIL F
Length:291
Mass (Da):32,161
Last modified:March 1, 2002 - v1
Checksum:iBDBDFF2732CD7D5D
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AL591975 Genomic DNA. Translation: CAC98569.1.
PIRiAC1136.
RefSeqiNP_464018.1. NC_003210.1.
WP_003725703.1. NC_003210.1.

Genome annotation databases

EnsemblBacteriaiCAC98569; CAC98569; CAC98569.
GeneIDi985396.
KEGGilmo:lmo0490.
PATRICi20310047. VBILisMon69206_0509.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AL591975 Genomic DNA. Translation: CAC98569.1.
PIRiAC1136.
RefSeqiNP_464018.1. NC_003210.1.
WP_003725703.1. NC_003210.1.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
3TNLX-ray1.45A/B/C/D1-291[»]
3TOZX-ray2.20A/B/C/D/E/F/G/H1-291[»]
ProteinModelPortaliQ8Y9N5.
SMRiQ8Y9N5.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi169963.lmo0490.

Proteomic databases

PaxDbiQ8Y9N5.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiCAC98569; CAC98569; CAC98569.
GeneIDi985396.
KEGGilmo:lmo0490.
PATRICi20310047. VBILisMon69206_0509.

Phylogenomic databases

eggNOGiENOG4105E2X. Bacteria.
COG0169. LUCA.
HOGENOMiHOG000237875.
KOiK00014.
OMAiSIFARND.
PhylomeDBiQ8Y9N5.

Enzyme and pathway databases

UniPathwayiUPA00053; UER00087.

Family and domain databases

Gene3Di3.40.50.720. 1 hit.
HAMAPiMF_00222. Shikimate_DH_AroE. 1 hit.
InterProiIPR016040. NAD(P)-bd_dom.
IPR011342. Shikimate_DH.
IPR013708. Shikimate_DH-bd_N.
IPR022893. Shikimate_DH_fam.
[Graphical view]
PfamiPF08501. Shikimate_dh_N. 1 hit.
[Graphical view]
SUPFAMiSSF51735. SSF51735. 1 hit.
TIGRFAMsiTIGR00507. aroE. 1 hit.
ProtoNetiSearch...

Entry informationi

Entry nameiAROE_LISMO
AccessioniPrimary (citable) accession number: Q8Y9N5
Entry historyi
Integrated into UniProtKB/Swiss-Prot: October 19, 2002
Last sequence update: March 1, 2002
Last modified: November 30, 2016
This is version 104 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  3. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.