Reviewed, UniProtKB/Swiss-Prot Q8Y9L8 (KPRS2_LISMO)

Names and origin

Protein names

Recommended name:
    Ribose-phosphate pyrophosphokinase 2
      Short name=RPPK 2
    EC=2.7.6.1
Alternative name(s):
    Phosphoribosyl pyrophosphate synthetase 2
      Short name=P-Rib-PP synthetase 2
      Short name=PRPP synthetase 2

Gene names

Name:	prs2
Ordered Locus Names:	lmo0509

Organism

Listeria monocytogenes [Complete proteome] [HAMAP]

Taxonomic identifier

1639 [NCBI]

Taxonomic lineage

Bacteria › Firmicutes › Bacillales › Listeriaceae › Listeria

Protein attributes

Sequence length	311 AA.
Sequence status	Complete.
Protein existence	Inferred from homology.

General annotation (Comments)

Catalytic activity	ATP + D-ribose 5-phosphate = AMP + 5-phospho-alpha-D-ribose 1-diphosphate. HAMAP MF_00583
Cofactor	Binds 1 magnesium ion per subunit By similarity. HAMAP MF_00583
Pathway	Metabolic intermediate biosynthesis; 5-phospho-alpha-D-ribose 1-diphosphate biosynthesis; 5-phospho-alpha-D-ribose 1-diphosphate from D-ribose 5-phosphate (route I): step 1/1. HAMAP MF_00583
Subcellular location	Cytoplasm By similarity HAMAP MF_00583.
Sequence similarities	Belongs to the ribose-phosphate pyrophosphokinase family.

Ontologies

Keywords
Biological process	Nucleotide biosynthesis
Cellular component	Cytoplasm
Ligand	ATP-binding Magnesium Metal-binding Nucleotide-binding
Molecular function	Kinase Transferase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	nucleoside metabolic process Inferred from electronic annotation. Source: InterPro ribonucleoside monophosphate biosynthetic process Inferred from electronic annotation. Source: HAMAP
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW kinase activity Inferred from electronic annotation. Source: UniProtKB-KW magnesium ion binding Inferred from electronic annotation. Source: HAMAP ribose phosphate diphosphokinase activity Inferred from electronic annotation. Source: HAMAP
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 311	311	Ribose-phosphate pyrophosphokinase 2 HAMAP MF_00583		PRO_0000141158
Regions
	Region	212 – 225	14	Binding of phosphoribosylpyrophosphate Potential
Sites
	Metal binding	129	1	Magnesium Potential
	Metal binding	131	1	Magnesium Potential
	Metal binding	140	1	Magnesium Potential
	Metal binding	144	1	Magnesium Potential

Sequences

	Sequence		Length	Mass (Da)
	Q8Y9L8-1 [UniParc]. Last modified March 1, 2002. Version 1. Checksum: 06B5C703555AD1C7	FASTA	311	34,345
	10 20 30 40 50 60 MAGKMKLFSV TSERPLATKI ADYLDIPLCE VELQKFSDGE VKINIEESIR GTNAYVVQSM 70 80 90 100 110 120 NSNVNERLME LLIMVDALKR ASVHSINIIM PYYGYARQDR KARSREPITA KLMANLIQRA 130 140 150 160 170 180 GANRLITVDL HAAQIQGFFN IPIDHLSAIP LIGDYLIENY GEKDVVVVAP DHSGVVRARR 190 200 210 220 230 240 IADRLNAPIA ILNRKPRPHE DEIMSVIGDV KGKVAIVVDD IIDTGVRATT SADILLEKGA 250 260 270 280 290 300 VEVIACATHS VMAGNATERL QNSNIKEVIT SDSIDLPEDK QFDKLTTISI GRILGRAIEG 310 VQENRSLHPL F

References

[1]

"Comparative genomics of Listeria species." Glaser P., Frangeul L., Buchrieser C., Rusniok C., Amend A., Baquero F., Berche P., Bloecker H., Brandt P., Chakraborty T., Charbit A., Chetouani F., Couve E., de Daruvar A., Dehoux P., Domann E., Dominguez-Bernal G., Duchaud E. , Durant L., Dussurget O., Entian K.-D., Fsihi H., Garcia-del Portillo F., Garrido P., Gautier L., Goebel W., Gomez-Lopez N., Hain T., Hauf J., Jackson D., Jones L.-M., Kaerst U., Kreft J., Kuhn M., Kunst F., Kurapkat G., Madueno E., Maitournam A., Mata Vicente J., Ng E., Nedjari H., Nordsiek G., Novella S., de Pablos B., Perez-Diaz J.-C., Purcell R., Remmel B., Rose M., Schlueter T., Simoes N., Tierrez A., Vazquez-Boland J.-A., Voss H., Wehland J., Cossart P. Science 294:849-852(2001) [PubMed: 11679669] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC BAA-679 / EGD-e / Serovar 1/2a.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AL591975 Genomic DNA. Translation: CAC98588.1.
PIR	AF1138.
RefSeq	NP_464037.1.
3D structure databases
SMR	Q8Y9L8. Positions 4-311.
ModBase	Search...
Genome annotation databases
GeneID	985257.
KEGG	lmo:lmo0509.
Organism-specific databases
ListiList	LMO00509.
CMR	Search...
Phylogenomic databases
HOGENOM	HBG519284.
OMA	RIKEVIT.
Enzyme and pathway databases
BioCyc	LMON169963:LMO0509-MONOMER.
BRENDA	2.7.6.1. 96770.
Family and domain databases
HAMAP	MF_00583_B. RibP_PPkinase_B. [Tree]
InterPro	IPR000842. PRib-PP_synthetase_CS. IPR000836. PRibTrfase. IPR005946. PRPP_kinase. [Graphical view]
Pfam	PF00156. Pribosyltran. 1 hit. [Graphical view]
TIGRFAMs	TIGR01251. ribP_PPkin. 1 hit.
PROSITE	PS00114. PRPP_SYNTHETASE. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

KPRS2_LISMO

Accession

Primary (citable) accession number: Q8Y9L8

Entry history

Integrated into UniProtKB/Swiss-Prot:	October 25, 2002
Last sequence update:	March 1, 2002
Last modified:	February 9, 2010
This is version 48 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)