Q8Y832 (ISPD2_LISMO) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Putative 2-C-methyl-D-erythritol 4-phosphate cytidylyltransferase 2
EC=2.7.7.60

Alternative name(s):
4-diphosphocytidyl-2C-methyl-D-erythritol synthase 2
MEP cytidylyltransferase 2
Short name=MCT 2

Gene names

Name:	ispD2
Ordered Locus Names:	lmo1086

Organism

Listeria monocytogenes

Taxonomic identifier

1639 [NCBI]

Taxonomic lineage

Bacteria › Firmicutes › Bacillales › Listeriaceae › Listeria

Protein attributes

Sequence length	236 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Catalyzes the formation of 4-diphosphocytidyl-2-C-methyl-D-erythritol from CTP and 2-C-methyl-D-erythritol 4-phosphate (MEP) By similarity. HAMAP MF_00108
Catalytic activity	CTP + 2-C-methyl-D-erythritol 4-phosphate = diphosphate + 4-(cytidine 5'-diphospho)-2-C-methyl-D-erythritol. HAMAP MF_00108
Pathway	Isoprenoid biosynthesis; isopentenyl diphosphate biosynthesis via DXP pathway; isopentenyl diphosphate from 1-deoxy-D-xylulose 5-phosphate: step 2/6. HAMAP MF_00108
Sequence similarities	Belongs to the IspD family.

Ontologies

Keywords
Biological process	Isoprene biosynthesis
Molecular function	Nucleotidyltransferase Transferase
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological process	isoprenoid biosynthetic process Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	2-C-methyl-D-erythritol 4-phosphate cytidylyltransferase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 236	236	Putative 2-C-methyl-D-erythritol 4-phosphate cytidylyltransferase 2 HAMAP MF_00108		PRO_0000075588
Sites
	Site	14	1	Transition state stabilizer By similarity
	Site	22	1	Transition state stabilizer By similarity
	Site	159	1	Positions MEP for the nucleophilic attack By similarity
	Site	216	1	Positions MEP for the nucleophilic attack By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q8Y832 [UniParc]. Last modified March 1, 2002. Version 1. Checksum: ACD3CE124E9A5098	FASTA	236	26,712
	10 20 30 40 50 60 MIYAEILAGG KGTRMGNVNM PKQYLPLKGK PIIVHTIEKF ILNDRFEKII IATPKDWINH 70 80 90 100 110 120 TQDIIKKYIF DSRVIVIEGG TDRNETIMNG IRYVEKEFGL NEDDIIVTHD AVRPFITHRI 130 140 150 160 170 180 IEENIDMALE FGSVDTVIPA VDTIVESTNH DFITDIPVRG NIYQGQTPQS FNMKTIQKHY 190 200 210 220 230 NNLTDDEKQI LTDACKICLL AGEKVKLVNG GISNIKITTP YDLKVANAIV QERINS

References

[1]

"Comparative genomics of Listeria species." Glaser P., Frangeul L., Buchrieser C., Rusniok C., Amend A., Baquero F., Berche P., Bloecker H., Brandt P., Chakraborty T., Charbit A., Chetouani F., Couve E., de Daruvar A., Dehoux P., Domann E., Dominguez-Bernal G., Duchaud E. , Durant L., Dussurget O., Entian K.-D., Fsihi H., Garcia-del Portillo F., Garrido P., Gautier L., Goebel W., Gomez-Lopez N., Hain T., Hauf J., Jackson D., Jones L.-M., Kaerst U., Kreft J., Kuhn M., Kunst F., Kurapkat G., Madueno E., Maitournam A., Mata Vicente J., Ng E., Nedjari H., Nordsiek G., Novella S., de Pablos B., Perez-Diaz J.-C., Purcell R., Remmel B., Rose M., Schlueter T., Simoes N., Tierrez A., Vazquez-Boland J.-A., Voss H., Wehland J., Cossart P. Science 294:849-852(2001) [PubMed: 11679669] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC BAA-679 / EGD-e / Serovar 1/2a.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AL591977 Genomic DNA. Translation: CAC99164.1.
PIR	AF1210.
RefSeq	NP_464611.1. NC_003210.1.
3D structure databases
ProteinModelPortal	Q8Y832.
ModBase	Search...
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	986270.
GenomeReviews	Gene locus lmo1086 in contig AL591824_GR.
KEGG	lmo:lmo1086.
PATRIC	20311277. VBILisMon69206_1116.
Organism-specific databases
GenoList	LMO1086.
Phylogenomic databases
HOGENOM	HBG672839.
OMA	GELFNIK.
ProtClustDB	PRK00155.
Enzyme and pathway databases
BioCyc	LMON169963:LMO1086-MONOMER.
Family and domain databases
HAMAP	MF_00108. IspD. [Tree]
InterPro	IPR001228. ISPD_synthase. IPR018294. ISPD_synthase_CS. [Graphical view]
KO	K00991.
Pfam	PF01128. IspD. 1 hit. [Graphical view]
PROSITE	PS01295. ISPD. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

ISPD2_LISMO

Accession

Primary (citable) accession number: Q8Y832

Entry history

Integrated into UniProtKB/Swiss-Prot:	August 31, 2004
Last sequence update:	March 1, 2002
Last modified:	January 25, 2012
This is version 51 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program