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Protein

Methylenetetrahydrofolate--tRNA-(uracil-5-)-methyltransferase TrmFO

Gene

trmFO

Organism
Listeria monocytogenes serovar 1/2a (strain ATCC BAA-679 / EGD-e)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Protein inferred from homologyi

Functioni

Catalyzes the folate-dependent formation of 5-methyl-uridine at position 54 (M-5-U54) in all tRNAs.UniRule annotation

Catalytic activityi

5,10-methylenetetrahydrofolate + uracil(54) in tRNA + FADH2 = tetrahydrofolate + 5-methyluracil(54) in tRNA + FAD.UniRule annotation

Cofactori

FADUniRule annotation

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Nucleotide bindingi9 – 146FADUniRule annotation

GO - Molecular functioni

Complete GO annotation...

Keywords - Molecular functioni

Methyltransferase, Transferase

Keywords - Biological processi

tRNA processing

Keywords - Ligandi

FAD, Flavoprotein

Enzyme and pathway databases

BioCyciLMON169963:LMO1276-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
Methylenetetrahydrofolate--tRNA-(uracil-5-)-methyltransferase TrmFOUniRule annotation (EC:2.1.1.74UniRule annotation)
Alternative name(s):
Folate-dependent tRNA (uracil-5-)-methyltransferaseUniRule annotation
Folate-dependent tRNA(M-5-U54)-methyltransferaseUniRule annotation
Gene namesi
Name:trmFOUniRule annotation
Synonyms:gid
Ordered Locus Names:lmo1276
OrganismiListeria monocytogenes serovar 1/2a (strain ATCC BAA-679 / EGD-e)
Taxonomic identifieri169963 [NCBI]
Taxonomic lineageiBacteriaFirmicutesBacilliBacillalesListeriaceaeListeria
ProteomesiUP000000817 Componenti: Chromosome

Organism-specific databases

GenoListiLMO1276.

Subcellular locationi

  • Cytoplasm UniRule annotation

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 434434Methylenetetrahydrofolate--tRNA-(uracil-5-)-methyltransferase TrmFOPRO_0000117251Add
BLAST

Interactioni

Protein-protein interaction databases

STRINGi169963.lmo1276.

Structurei

3D structure databases

ProteinModelPortaliQ8Y7K1.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the MnmG family. TrmFO subfamily.UniRule annotation

Phylogenomic databases

eggNOGiCOG1206.
HOGENOMiHOG000252054.
KOiK04094.
OMAiDYLNCPM.
OrthoDBiEOG6J74VT.
PhylomeDBiQ8Y7K1.

Family and domain databases

HAMAPiMF_01037. TrmFO.
InterProiIPR004417. Folate-dep_Ribothymidyl_synth.
IPR002218. GIDA-rel.
IPR020595. GIDA-rel_CS.
[Graphical view]
PfamiPF01134. GIDA. 1 hit.
[Graphical view]
TIGRFAMsiTIGR00137. gid_trmFO. 1 hit.
PROSITEiPS01281. GIDA_2. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q8Y7K1-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MEKSVNVIGA GLAGSEAVWQ LVNRGVKVDL YEMRPVKQTP AHHTDKFAEL
60 70 80 90 100
VCTNSLRANG LTNAVGVIKE EMRMLDSIII EAADKASVPA GGALAVDRHE
110 120 130 140 150
FSGYITDKVK NHPLVTVHTE EVTTIPEGPT IIATGPLTSP ALADEIKQLT
160 170 180 190 200
GEEYLYFYDA AAPIIEKDSI DMDKVYLKSR YDKGEAAYLN CPMSEEEFNA
210 220 230 240 250
FYEALVTAET AALKEFEKEV FFEGCMPIEV MAKRGIKTML FGPLKPVGLE
260 270 280 290 300
DPKTGKRPYA VLQLRQDDAA GTLYNMVGFQ THLKWGEQKR VFGMIPGLEN
310 320 330 340 350
AEIVRYGVMH RNTFINSPTV LEPTYQLKTR NDLFFAGQMT GVEGYVESAA
360 370 380 390 400
SGLAAGINAA NFIQEKELVV FPTETAIGSL AHYITSASKK SFQPMNVNFG
410 420 430
LFPELETKIR AKQERNEKLA ERALNAIKKV AEEL
Length:434
Mass (Da):47,899
Last modified:March 1, 2002 - v1
Checksum:i9A24FC605A7D8C76
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AL591978 Genomic DNA. Translation: CAC99354.1.
PIRiAD1234.
RefSeqiNP_464801.1. NC_003210.1.
WP_010989721.1. NC_003210.1.

Genome annotation databases

EnsemblBacteriaiCAC99354; CAC99354; CAC99354.
GeneIDi985107.
KEGGilmo:lmo1276.
PATRICi20311669. VBILisMon69206_1311.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AL591978 Genomic DNA. Translation: CAC99354.1.
PIRiAD1234.
RefSeqiNP_464801.1. NC_003210.1.
WP_010989721.1. NC_003210.1.

3D structure databases

ProteinModelPortaliQ8Y7K1.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi169963.lmo1276.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiCAC99354; CAC99354; CAC99354.
GeneIDi985107.
KEGGilmo:lmo1276.
PATRICi20311669. VBILisMon69206_1311.

Organism-specific databases

GenoListiLMO1276.

Phylogenomic databases

eggNOGiCOG1206.
HOGENOMiHOG000252054.
KOiK04094.
OMAiDYLNCPM.
OrthoDBiEOG6J74VT.
PhylomeDBiQ8Y7K1.

Enzyme and pathway databases

BioCyciLMON169963:LMO1276-MONOMER.

Family and domain databases

HAMAPiMF_01037. TrmFO.
InterProiIPR004417. Folate-dep_Ribothymidyl_synth.
IPR002218. GIDA-rel.
IPR020595. GIDA-rel_CS.
[Graphical view]
PfamiPF01134. GIDA. 1 hit.
[Graphical view]
TIGRFAMsiTIGR00137. gid_trmFO. 1 hit.
PROSITEiPS01281. GIDA_2. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

  1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: ATCC BAA-679 / EGD-e.

Entry informationi

Entry nameiTRMFO_LISMO
AccessioniPrimary (citable) accession number: Q8Y7K1
Entry historyi
Integrated into UniProtKB/Swiss-Prot: November 28, 2002
Last sequence update: March 1, 2002
Last modified: July 22, 2015
This is version 83 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.