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Q8Y7K1 (TRMFO_LISMO) Reviewed, UniProtKB/Swiss-Prot

Last modified July 9, 2014. Version 76. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (1) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Methylenetetrahydrofolate--tRNA-(uracil-5-)-methyltransferase TrmFO

EC=2.1.1.74
Alternative name(s):
Folate-dependent tRNA (uracil-5-)-methyltransferase
Folate-dependent tRNA(M-5-U54)-methyltransferase
Gene names
Name:trmFO
Synonyms:gid
Ordered Locus Names:lmo1276
OrganismListeria monocytogenes serovar 1/2a (strain ATCC BAA-679 / EGD-e) [Reference proteome] [HAMAP]
Taxonomic identifier169963 [NCBI]
Taxonomic lineageBacteriaFirmicutesBacilliBacillalesListeriaceaeListeria

Protein attributes

Sequence length434 AA.
Sequence statusComplete.
Protein existenceInferred from homology

General annotation (Comments)

Function

Catalyzes the folate-dependent formation of 5-methyl-uridine at position 54 (M-5-U54) in all tRNAs By similarity. HAMAP-Rule MF_01037

Catalytic activity

5,10-methylenetetrahydrofolate + uracil54 in tRNA + FADH2 = tetrahydrofolate + 5-methyluracil54 in tRNA + FAD. HAMAP-Rule MF_01037

Cofactor

FAD By similarity. HAMAP-Rule MF_01037

Subcellular location

Cytoplasm By similarity HAMAP-Rule MF_01037.

Sequence similarities

Belongs to the MnmG family. TrmFO subfamily.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 434434Methylenetetrahydrofolate--tRNA-(uracil-5-)-methyltransferase TrmFO HAMAP-Rule MF_01037
PRO_0000117251

Regions

Nucleotide binding9 – 146FAD By similarity

Sequences

Sequence LengthMass (Da)Tools
Q8Y7K1 [UniParc].

Last modified March 1, 2002. Version 1.
Checksum: 9A24FC605A7D8C76

FASTA43447,899
        10         20         30         40         50         60 
MEKSVNVIGA GLAGSEAVWQ LVNRGVKVDL YEMRPVKQTP AHHTDKFAEL VCTNSLRANG 

        70         80         90        100        110        120 
LTNAVGVIKE EMRMLDSIII EAADKASVPA GGALAVDRHE FSGYITDKVK NHPLVTVHTE 

       130        140        150        160        170        180 
EVTTIPEGPT IIATGPLTSP ALADEIKQLT GEEYLYFYDA AAPIIEKDSI DMDKVYLKSR 

       190        200        210        220        230        240 
YDKGEAAYLN CPMSEEEFNA FYEALVTAET AALKEFEKEV FFEGCMPIEV MAKRGIKTML 

       250        260        270        280        290        300 
FGPLKPVGLE DPKTGKRPYA VLQLRQDDAA GTLYNMVGFQ THLKWGEQKR VFGMIPGLEN 

       310        320        330        340        350        360 
AEIVRYGVMH RNTFINSPTV LEPTYQLKTR NDLFFAGQMT GVEGYVESAA SGLAAGINAA 

       370        380        390        400        410        420 
NFIQEKELVV FPTETAIGSL AHYITSASKK SFQPMNVNFG LFPELETKIR AKQERNEKLA 

       430 
ERALNAIKKV AEEL 

« Hide

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
AL591978 Genomic DNA. Translation: CAC99354.1.
PIRAD1234.
RefSeqNP_464801.1. NC_003210.1.

3D structure databases

ProteinModelPortalQ8Y7K1.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

STRING169963.lmo1276.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaCAC99354; CAC99354; CAC99354.
GeneID985107.
KEGGlmo:lmo1276.
PATRIC20311669. VBILisMon69206_1311.

Organism-specific databases

GenoListLMO1276.

Phylogenomic databases

eggNOGCOG1206.
HOGENOMHOG000252054.
KOK04094.
OMAEIYFEGC.
OrthoDBEOG6J74VT.
PhylomeDBQ8Y7K1.

Enzyme and pathway databases

BioCycLMON169963:LMO1276-MONOMER.

Family and domain databases

HAMAPMF_01037. TrmFO.
InterProIPR004417. Folate-dep_Ribothymidyl_synth.
IPR002218. GIDA-rel.
IPR020595. GIDA-rel_CS.
[Graphical view]
PfamPF01134. GIDA. 1 hit.
[Graphical view]
TIGRFAMsTIGR00137. gid_trmFO. 1 hit.
PROSITEPS01281. GIDA_2. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameTRMFO_LISMO
AccessionPrimary (citable) accession number: Q8Y7K1
Entry history
Integrated into UniProtKB/Swiss-Prot: November 28, 2002
Last sequence update: March 1, 2002
Last modified: July 9, 2014
This is version 76 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families