Reviewed, UniProtKB/Swiss-Prot Q8Y6Z6 (LDH2_LISMO)

Names and origin

Protein names

Recommended name:
    L-lactate dehydrogenase 2
      Short name=L-LDH 2
    EC=1.1.1.27

Gene names

Name:	ldh2
Ordered Locus Names:	lmo1534

Organism

Listeria monocytogenes [Complete proteome] [HAMAP]

Taxonomic identifier

1639 [NCBI]

Taxonomic lineage

Bacteria › Firmicutes › Bacillales › Listeriaceae › Listeria

Protein attributes

Sequence length	311 AA.
Sequence status	Complete.
Protein existence	Inferred from homology.

General annotation (Comments)

Catalytic activity	(S)-lactate + NAD+ = pyruvate + NADH. HAMAP MF_00488
Pathway	Fermentation; pyruvate fermentation to lactate; (S)-lactate from pyruvate: step 1/1. HAMAP MF_00488
Subunit structure	Homotetramer By similarity. HAMAP MF_00488
Subcellular location	Cytoplasm By similarity HAMAP MF_00488.
Sequence similarities	Belongs to the LDH/MDH superfamily. LDH family.

Ontologies

Keywords
Biological process	Glycolysis
Cellular component	Cytoplasm
Ligand	NAD
Molecular function	Oxidoreductase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	anaerobic glycolysis Inferred from electronic annotation. Source: HAMAP oxidation reduction Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	L-lactate dehydrogenase activity Inferred from electronic annotation. Source: HAMAP binding Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 311	311	L-lactate dehydrogenase 2 HAMAP MF_00488		PRO_0000168366
Regions
	Nucleotide binding	12 – 40	29	NAD By similarity
Sites
	Active site	177	1	Proton acceptor By similarity
	Binding site	90	1	Substrate By similarity
	Binding site	122	1	NAD or substrate By similarity
	Binding site	153	1	Substrate By similarity
	Binding site	230	1	Substrate By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q8Y6Z6-1 [UniParc]. Last modified March 1, 2002. Version 1. Checksum: D7DB065307C8E301	FASTA	311	33,741
	10 20 30 40 50 60 MKPRKVMIIG AGNVGTAAAH AFVNQKFVEE LILVDLNKER VEGNRKDLAD AAAFMPGKMD 70 80 90 100 110 120 ITVRDASDCA DVDIAVITVT AGPLKEGQTR LDELRSTSRI VSGIVPEMMK GGFNGIFLIA 130 140 150 160 170 180 TNPCDIITYQ VWKLSGLPRE RVLGTGVWLD TTRLRRLLAE KLDIAAQSID AFILGEHGDS 190 200 210 220 230 240 QFPVWSHSSI YGKPVNEYSL EKLGESLDLK QIGETARDTG FEIYHQKGCT EYGIGGTIVE 250 260 270 280 290 300 ICRHIFSGSQ RALTVSCVLD GEYGESGLAI GVPAVLSQNG VKEIISLKLD EQEQQAFANS 310 VSVIKKSIAS I

References

[1]

"Comparative genomics of Listeria species." Glaser P., Frangeul L., Buchrieser C., Rusniok C., Amend A., Baquero F., Berche P., Bloecker H., Brandt P., Chakraborty T., Charbit A., Chetouani F., Couve E., de Daruvar A., Dehoux P., Domann E., Dominguez-Bernal G., Duchaud E. , Durant L., Dussurget O., Entian K.-D., Fsihi H., Garcia-del Portillo F., Garrido P., Gautier L., Goebel W., Gomez-Lopez N., Hain T., Hauf J., Jackson D., Jones L.-M., Kaerst U., Kreft J., Kuhn M., Kunst F., Kurapkat G., Madueno E., Maitournam A., Mata Vicente J., Ng E., Nedjari H., Nordsiek G., Novella S., de Pablos B., Perez-Diaz J.-C., Purcell R., Remmel B., Rose M., Schlueter T., Simoes N., Tierrez A., Vazquez-Boland J.-A., Voss H., Wehland J., Cossart P. Science 294:849-852(2001) [PubMed: 11679669] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC BAA-679 / EGD-e / Serovar 1/2a.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AL591979 Genomic DNA. Translation: CAC99612.1.
PIR	AF1266.
RefSeq	NP_465059.1.
3D structure databases
SMR	Q8Y6Z6. Positions 2-310.
ModBase	Search...
Genome annotation databases
GeneID	987808.
KEGG	lmo:lmo1534.
Organism-specific databases
ListiList	LMO01534.
CMR	Search...
Phylogenomic databases
HOGENOM	HBG566126.
OMA	REASDCA.
Enzyme and pathway databases
BioCyc	LMON169963:LMO1534-MONOMER.
BRENDA	1.1.1.27. 96770.
Family and domain databases
HAMAP	MF_00488. Lactate_dehydrog. [Tree]
InterPro	IPR001557. L-lactate/malate_DH. IPR011304. L-lactate_DH. IPR018177. L-lactate_DH_AS. IPR001236. Lactate/malate_DH. IPR015955. Lactate_DH/Glyco_Ohase_4_C. IPR016040. NAD(P)-bd_dom. [Graphical view]
Gene3D	G3DSA:3.90.110.10. lact_mal_DH. 1 hit. G3DSA:3.40.50.720. NAD(P)-bd. 1 hit.
Pfam	PF02866. Ldh_1_C. 1 hit. PF00056. Ldh_1_N. 1 hit. [Graphical view]
PIRSF	PIRSF000102. Lac_mal_DH. 1 hit.
PRINTS	PR00086. LLDHDRGNASE.
TIGRFAMs	TIGR01771. L-LDH-NAD. 1 hit.
PROSITE	PS00064. L_LDH. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

LDH2_LISMO

Accession

Primary (citable) accession number: Q8Y6Z6

Entry history

Integrated into UniProtKB/Swiss-Prot:	June 21, 2004
Last sequence update:	March 1, 2002
Last modified:	February 9, 2010
This is version 53 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)