Reviewed, UniProtKB/Swiss-Prot Q8Y6C6 (PUR2_LISMO)

Names and origin

Protein names

Recommended name:
    Phosphoribosylamine--glycine ligase
    EC=6.3.4.13
Alternative name(s):
    GARS
    Glycinamide ribonucleotide synthetase
    Phosphoribosylglycinamide synthetase

Gene names

Name:	purD
Ordered Locus Names:	lmo1764

Organism

Listeria monocytogenes [Complete proteome] [HAMAP]

Taxonomic identifier

1639 [NCBI]

Taxonomic lineage

Bacteria › Firmicutes › Bacillales › Listeriaceae › Listeria

Protein attributes

Sequence length	420 AA.
Sequence status	Complete.
Protein existence	Inferred from homology.

General annotation (Comments)

Catalytic activity	ATP + 5-phospho-D-ribosylamine + glycine = ADP + phosphate + N(1)-(5-phospho-D-ribosyl)glycinamide. HAMAP MF_00138
Cofactor	Binds 1 magnesium or manganese ion per subunit By similarity. HAMAP MF_00138
Pathway	Purine metabolism; IMP biosynthesis via de novo pathway; N(1)-(5-phospho-D-ribosyl)glycinamide from 5-phospho-alpha-D-ribose 1-diphosphate: step 2/2. HAMAP MF_00138
Sequence similarities	Belongs to the GARS family. Contains 1 ATP-grasp domain.

Ontologies

Keywords
Biological process	Purine biosynthesis
Ligand	ATP-binding Magnesium Manganese Metal-binding Nucleotide-binding
Molecular function	Ligase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	purine base biosynthetic process Inferred from electronic annotation. Source: InterPro purine nucleotide biosynthetic process Inferred from electronic annotation. Source: HAMAP
Molecular function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW magnesium ion binding Inferred from electronic annotation. Source: UniProtKB-KW manganese ion binding Inferred from electronic annotation. Source: UniProtKB-KW phosphoribosylamine-glycine ligase activity Inferred from electronic annotation. Source: HAMAP
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 420	420	Phosphoribosylamine--glycine ligase HAMAP MF_00138		PRO_0000151462
Regions
	Domain	108 – 314	207	ATP-grasp
	Nucleotide binding	134 – 195	62	ATP By similarity
Sites
	Metal binding	284	1	Magnesium or manganese By similarity
	Metal binding	286	1	Magnesium or manganese By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q8Y6C6-1 [UniParc]. Last modified March 27, 2002. Version 1. Checksum: ED0CD36347589C3F	FASTA	420	45,383
	10 20 30 40 50 60 MNLLVVGSGG REHAISKKLL ASNNVEKVFC APGNDGMRLD DIQLVAISET DKAGLIDFAK 70 80 90 100 110 120 KAEIAFVIVG PEVPLLEGVV DALEEAGIKA FGPKANAALI EGSKDFAKQF MEKYAIPTAA 130 140 150 160 170 180 SRTFTDYAEA KAYLNERGVP IVIKADGLAA GKGVTVALEM EEAVLALKDM MLEEKFGDAS 190 200 210 220 230 240 LKVVIEDFLA GEEFSLMAFV NGEEVYPMSI AQDHKRAYEG DKGPNTGGMG AYSPVPHISE 250 260 270 280 290 300 AVIEEAVQKI LLPTAKGMVK EGRYFRGILY AGLILTADGP KVIEFNARFG DPETQVVLPR 310 320 330 340 350 360 LESDFAALID ALLHNEKPDV RFKKTGLTLG VVLASAGYPE HYEKGNKLTG LNDIAEDVAI 370 380 390 400 410 420 YHAGTKQDEN GDFISDGGRV LLLAKEAETM SDARTLLYPE MQKLDNPNFF YRIDIGTKAE

References

[1]

"Comparative genomics of Listeria species." Glaser P., Frangeul L., Buchrieser C., Rusniok C., Amend A., Baquero F., Berche P., Bloecker H., Brandt P., Chakraborty T., Charbit A., Chetouani F., Couve E., de Daruvar A., Dehoux P., Domann E., Dominguez-Bernal G., Duchaud E. , Durant L., Dussurget O., Entian K.-D., Fsihi H., Garcia-del Portillo F., Garrido P., Gautier L., Goebel W., Gomez-Lopez N., Hain T., Hauf J., Jackson D., Jones L.-M., Kaerst U., Kreft J., Kuhn M., Kunst F., Kurapkat G., Madueno E., Maitournam A., Mata Vicente J., Ng E., Nedjari H., Nordsiek G., Novella S., de Pablos B., Perez-Diaz J.-C., Purcell R., Remmel B., Rose M., Schlueter T., Simoes N., Tierrez A., Vazquez-Boland J.-A., Voss H., Wehland J., Cossart P. Science 294:849-852(2001) [PubMed: 11679669] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC BAA-679 / EGD-e / Serovar 1/2a.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AL591981 Genomic DNA. Translation: CAC99842.1.
PIR	AD1295.
RefSeq	NP_465289.1.
3D structure databases
SMR	Q8Y6C6. Positions 1-419.
ModBase	Search...
Genome annotation databases
GeneID	985980.
KEGG	lmo:lmo1764.
Organism-specific databases
ListiList	LMO01764.
CMR	Search...
Phylogenomic databases
HOGENOM	HBG404060.
OMA	FSLMAFV.
Enzyme and pathway databases
BioCyc	LMON169963:LMO1764-MONOMER.
BRENDA	6.3.4.13. 96770.
Family and domain databases
HAMAP	MF_00138. GARS. [Tree]
InterPro	IPR011761. ATP-grasp. IPR013815. ATP_grasp_subdomain_1. IPR013816. ATP_grasp_subdomain_2. IPR013817. Pre-ATP_grasp. IPR016185. PreATP-grasp-like. IPR020561. PRibGlycinamid_synth_ATP-grasp. IPR000115. PRibGlycinamide_synth. IPR020560. PRibGlycinamide_synth_C-dom. IPR020559. PRibGlycinamide_synth_CS. IPR020562. PRibGlycinamide_synth_N-dom. IPR011054. Rudment_hybrid_motif. [Graphical view]
Gene3D	G3DSA:3.30.1490.20. ATP_grasp_subdomain_1. 1 hit. G3DSA:3.30.470.20. ATP_grasp_subdomain_2. 1 hit. G3DSA:3.40.50.20. Pre-ATP_grasp. 1 hit.
Pfam	PF01071. GARS_A. 1 hit. PF02843. GARS_C. 1 hit. PF02844. GARS_N. 1 hit. [Graphical view]
TIGRFAMs	TIGR00877. purD. 1 hit.
PROSITE	PS50975. ATP_GRASP. 1 hit. PS00184. GARS. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

PUR2_LISMO

Accession

Primary (citable) accession number: Q8Y6C6

Entry history

Integrated into UniProtKB/Swiss-Prot:	March 27, 2002
Last sequence update:	March 27, 2002
Last modified:	February 9, 2010
This is version 54 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)