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Q8Y678

- STP1_LISMO

UniProt

Q8Y678 - STP1_LISMO

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Protein
Serine/threonine phosphatase stp
Gene
stp, lmo1821
Organism
Listeria monocytogenes serovar 1/2a (strain ATCC BAA-679 / EGD-e)
Status
Reviewed - Annotation score: 4 out of 5 - Experimental evidence at protein leveli

Functioni

Protein phosphatase that dephosphorylates EF-Tu.1 Publication

Catalytic activityi

[a protein]-serine/threonine phosphate + H2O = [a protein]-serine/threonine + phosphate.

Cofactori

Binds 2 manganese ions per subunit.1 Publication

Enzyme regulationi

Activity not affected by inhibitors of phosphatases of the PPP family such as okadaic acid and cypermethrin, or by inhibitors of phosphatases of the PTP family such as sodium orthovanadate.

Kineticsi

  1. KM=3.77 µM for MBP1 Publication
  2. KM=0.62 µM for EF-Tu

Vmax=120.48 pmol/min/µg enzyme toward MBP

Vmax=2.94 pmol/min/µg enzyme toward EF-Tu

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Metal bindingi36 – 361Manganese 1 By similarity
Metal bindingi36 – 361Manganese 2 By similarity
Metal bindingi37 – 371Manganese 1; via carbonyl oxygen By similarity
Metal bindingi194 – 1941Manganese 2 By similarity
Metal bindingi233 – 2331Manganese 2 By similarity

GO - Molecular functioni

  1. metal ion binding Source: UniProtKB-KW
  2. phosphoprotein phosphatase activity Source: UniProtKB-KW
Complete GO annotation...

GO - Biological processi

    Complete GO annotation...

    Keywords - Molecular functioni

    Hydrolase, Protein phosphatase

    Keywords - Ligandi

    Manganese, Metal-binding

    Enzyme and pathway databases

    BioCyciLMON169963:LMO1821-MONOMER.

    Protein family/group databases

    PptaseDBiP3D0611176.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Serine/threonine phosphatase stp (EC:3.1.3.16)
    Gene namesi
    Name:stp
    Ordered Locus Names:lmo1821
    OrganismiListeria monocytogenes serovar 1/2a (strain ATCC BAA-679 / EGD-e)
    Taxonomic identifieri169963 [NCBI]
    Taxonomic lineageiBacteriaFirmicutesBacilliBacillalesListeriaceaeListeria
    ProteomesiUP000000817: Chromosome

    Organism-specific databases

    GenoListiLMO1821.

    Subcellular locationi

    Cytoplasm. Membrane; Peripheral membrane protein 1 Publication

    GO - Cellular componenti

    1. cytoplasm Source: UniProtKB-SubCell
    2. membrane Source: UniProtKB-SubCell
    Complete GO annotation...

    Keywords - Cellular componenti

    Cytoplasm, Membrane

    Pathology & Biotechi

    Disruption phenotypei

    The stp-disrupted strain is 4 times less virulent than the wild strain, demonstrating that stp is required for full virulence of L.monocytogenes in BALB/c mice.1 Publication

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 252252Serine/threonine phosphatase stp
    PRO_0000363063Add
    BLAST

    Interactioni

    Protein-protein interaction databases

    STRINGi169963.lmo1821.

    Structurei

    3D structure databases

    ProteinModelPortaliQ8Y678.

    Family & Domainsi

    Domains and Repeats

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Domaini2 – 235234PP2C-like
    Add
    BLAST

    Sequence similaritiesi

    Belongs to the PP2C family.
    Contains 1 PP2C-like domain.

    Phylogenomic databases

    eggNOGiCOG0631.
    HOGENOMiHOG000235782.
    KOiK01090.
    OMAiHEECQGM.
    OrthoDBiEOG65N17S.
    PhylomeDBiQ8Y678.

    Family and domain databases

    Gene3Di3.60.40.10. 1 hit.
    InterProiIPR001932. PP2C-like_dom.
    IPR015655. Protein_Pase_2C.
    [Graphical view]
    PANTHERiPTHR13832. PTHR13832. 1 hit.
    PfamiPF00481. PP2C. 1 hit.
    [Graphical view]
    SMARTiSM00331. PP2C_SIG. 1 hit.
    SM00332. PP2Cc. 1 hit.
    [Graphical view]
    SUPFAMiSSF81606. SSF81606. 1 hit.

    Sequencei

    Sequence statusi: Complete.

    Q8Y678-1 [UniParc]FASTAAdd to Basket

    « Hide

    MHAEFRTDRG RIRHHNEDNG GVFENKDNQP IVIVADGMGG HRAGDVASEM    50
    AVRLLSDAWK ETTALLTAEE IETWLRKTIQ EVNKEIVLYA ESEMDLNGMG 100
    TTLVAAIMAQ SQVVIANVGD SRGYLLQNHV LRQLTEDHSL VHELLRTGEI 150
    SKEDAMNHPR KNILLRALGV EGKVEVDTFV VPFQTSDTLL LCSDGLTNMV 200
    PETEMEEILK SKRTLSEKAD VFITKANSYG GEDNITVLLV ERDLTQKGRD 250
    AS 252
    Length:252
    Mass (Da):28,026
    Last modified:March 1, 2002 - v1
    Checksum:i47EE5DA232A43338
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AL591981 Genomic DNA. Translation: CAC99899.1.
    PIRiAE1302.
    RefSeqiNP_465346.1. NC_003210.1.

    Genome annotation databases

    EnsemblBacteriaiCAC99899; CAC99899; CAC99899.
    GeneIDi985901.
    KEGGilmo:lmo1821.
    PATRICi20312835. VBILisMon69206_1866.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AL591981 Genomic DNA. Translation: CAC99899.1 .
    PIRi AE1302.
    RefSeqi NP_465346.1. NC_003210.1.

    3D structure databases

    ProteinModelPortali Q8Y678.
    ModBasei Search...

    Protein-protein interaction databases

    STRINGi 169963.lmo1821.

    Protein family/group databases

    PptaseDBi P3D0611176.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblBacteriai CAC99899 ; CAC99899 ; CAC99899 .
    GeneIDi 985901.
    KEGGi lmo:lmo1821.
    PATRICi 20312835. VBILisMon69206_1866.

    Organism-specific databases

    GenoListi LMO1821.

    Phylogenomic databases

    eggNOGi COG0631.
    HOGENOMi HOG000235782.
    KOi K01090.
    OMAi HEECQGM.
    OrthoDBi EOG65N17S.
    PhylomeDBi Q8Y678.

    Enzyme and pathway databases

    BioCyci LMON169963:LMO1821-MONOMER.

    Family and domain databases

    Gene3Di 3.60.40.10. 1 hit.
    InterProi IPR001932. PP2C-like_dom.
    IPR015655. Protein_Pase_2C.
    [Graphical view ]
    PANTHERi PTHR13832. PTHR13832. 1 hit.
    Pfami PF00481. PP2C. 1 hit.
    [Graphical view ]
    SMARTi SM00331. PP2C_SIG. 1 hit.
    SM00332. PP2Cc. 1 hit.
    [Graphical view ]
    SUPFAMi SSF81606. SSF81606. 1 hit.
    ProtoNeti Search...

    Publicationsi

    « Hide 'large scale' publications
    1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: ATCC BAA-679 / EGD-e.
    2. "Translation elongation factor EF-Tu is a target for Stp, a serine-threonine phosphatase involved in virulence of Listeria monocytogenes."
      Archambaud C., Gouin E., Pizarro-Cerda J., Cossart P., Dussurget O.
      Mol. Microbiol. 56:383-396(2005) [PubMed] [Europe PMC] [Abstract]
      Cited for: FUNCTION AS PHOSPHATASE, SUBCELLULAR LOCATION, COFACTOR, BIOPHYSICOCHEMICAL PROPERTIES, DISRUPTION PHENOTYPE, IDENTIFICATION OF SUBSTRATE.
      Strain: ATCC BAA-679 / EGD-e.

    Entry informationi

    Entry nameiSTP1_LISMO
    AccessioniPrimary (citable) accession number: Q8Y678
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: February 10, 2009
    Last sequence update: March 1, 2002
    Last modified: July 9, 2014
    This is version 71 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programProkaryotic Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3

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