Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Protein

Serine/threonine phosphatase stp

Gene

stp

Organism
Listeria monocytogenes serovar 1/2a (strain ATCC BAA-679 / EGD-e)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Protein phosphatase that dephosphorylates EF-Tu.1 Publication

Catalytic activityi

[a protein]-serine/threonine phosphate + H2O = [a protein]-serine/threonine + phosphate.

Cofactori

Mn2+1 PublicationNote: Binds 2 manganese ions per subunit.1 Publication

Enzyme regulationi

Activity not affected by inhibitors of phosphatases of the PPP family such as okadaic acid and cypermethrin, or by inhibitors of phosphatases of the PTP family such as sodium orthovanadate.

Kineticsi

  1. KM=3.77 µM for MBP1 Publication
  2. KM=0.62 µM for EF-Tu1 Publication
  1. Vmax=120.48 pmol/min/µg enzyme toward MBP1 Publication
  2. Vmax=2.94 pmol/min/µg enzyme toward EF-Tu1 Publication

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Metal bindingi36Manganese 1By similarity1
Metal bindingi36Manganese 2By similarity1
Metal bindingi37Manganese 1; via carbonyl oxygenBy similarity1
Metal bindingi194Manganese 2By similarity1
Metal bindingi233Manganese 2By similarity1

GO - Molecular functioni

Keywordsi

Molecular functionHydrolase, Protein phosphatase
LigandManganese, Metal-binding

Enzyme and pathway databases

BioCyciLMON169963:G1GTA-1949-MONOMER
SABIO-RKQ8Y678

Names & Taxonomyi

Protein namesi
Recommended name:
Serine/threonine phosphatase stp (EC:3.1.3.16)
Gene namesi
Name:stp
Ordered Locus Names:lmo1821
OrganismiListeria monocytogenes serovar 1/2a (strain ATCC BAA-679 / EGD-e)
Taxonomic identifieri169963 [NCBI]
Taxonomic lineageiBacteriaFirmicutesBacilliBacillalesListeriaceaeListeria
Proteomesi
  • UP000000817 Componenti: Chromosome

Subcellular locationi

GO - Cellular componenti

Keywords - Cellular componenti

Cytoplasm, Membrane

Pathology & Biotechi

Disruption phenotypei

The stp-disrupted strain is 4 times less virulent than the wild strain, demonstrating that stp is required for full virulence of L.monocytogenes in BALB/c mice.1 Publication

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00003630631 – 252Serine/threonine phosphatase stpAdd BLAST252

Proteomic databases

PaxDbiQ8Y678

Interactioni

Protein-protein interaction databases

STRINGi169963.lmo1821

Structurei

3D structure databases

ProteinModelPortaliQ8Y678
SMRiQ8Y678
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini2 – 242PPM-type phosphatasePROSITE-ProRule annotationAdd BLAST241

Sequence similaritiesi

Belongs to the PP2C family.Curated

Phylogenomic databases

eggNOGiENOG4108ZF3 Bacteria
COG0631 LUCA
HOGENOMiHOG000235782
KOiK20074
OMAiNNEDFHR
PhylomeDBiQ8Y678

Family and domain databases

CDDicd00143 PP2Cc, 1 hit
Gene3Di3.60.40.10, 1 hit
InterProiView protein in InterPro
IPR015655 PP2C
IPR036457 PPM-type_dom_sf
IPR001932 PPM-type_phosphatase_dom
PANTHERiPTHR13832 PTHR13832, 1 hit
SMARTiView protein in SMART
SM00331 PP2C_SIG, 1 hit
SM00332 PP2Cc, 1 hit
SUPFAMiSSF81606 SSF81606, 1 hit
PROSITEiView protein in PROSITE
PS51746 PPM_2, 1 hit

Sequencei

Sequence statusi: Complete.

Q8Y678-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MHAEFRTDRG RIRHHNEDNG GVFENKDNQP IVIVADGMGG HRAGDVASEM
60 70 80 90 100
AVRLLSDAWK ETTALLTAEE IETWLRKTIQ EVNKEIVLYA ESEMDLNGMG
110 120 130 140 150
TTLVAAIMAQ SQVVIANVGD SRGYLLQNHV LRQLTEDHSL VHELLRTGEI
160 170 180 190 200
SKEDAMNHPR KNILLRALGV EGKVEVDTFV VPFQTSDTLL LCSDGLTNMV
210 220 230 240 250
PETEMEEILK SKRTLSEKAD VFITKANSYG GEDNITVLLV ERDLTQKGRD

AS
Length:252
Mass (Da):28,026
Last modified:March 1, 2002 - v1
Checksum:i47EE5DA232A43338
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AL591981 Genomic DNA Translation: CAC99899.1
PIRiAE1302
RefSeqiNP_465346.1, NC_003210.1
WP_003723065.1, NC_003210.1

Genome annotation databases

EnsemblBacteriaiCAC99899; CAC99899; CAC99899
GeneIDi985901
KEGGilmo:lmo1821
PATRICifig|169963.11.peg.1866

Similar proteinsi

Entry informationi

Entry nameiSTP1_LISMO
AccessioniPrimary (citable) accession number: Q8Y678
Entry historyiIntegrated into UniProtKB/Swiss-Prot: February 10, 2009
Last sequence update: March 1, 2002
Last modified: March 28, 2018
This is version 93 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Cookie policy

We would like to use anonymized google analytics cookies to gather statistics on how uniprot.org is used in aggregate. Learn more

UniProt is an ELIXIR core data resource
Main funding by: National Institutes of Health