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Q8Y678

- STP1_LISMO

UniProt

Q8Y678 - STP1_LISMO

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Protein

Serine/threonine phosphatase stp

Gene

stp

Organism
Listeria monocytogenes serovar 1/2a (strain ATCC BAA-679 / EGD-e)
Status
Reviewed - Annotation score: 4 out of 5- Experimental evidence at protein leveli

Functioni

Protein phosphatase that dephosphorylates EF-Tu.1 Publication

Catalytic activityi

[a protein]-serine/threonine phosphate + H2O = [a protein]-serine/threonine + phosphate.

Cofactori

Mn2+1 PublicationNote: Binds 2 manganese ions per subunit.1 Publication

Enzyme regulationi

Activity not affected by inhibitors of phosphatases of the PPP family such as okadaic acid and cypermethrin, or by inhibitors of phosphatases of the PTP family such as sodium orthovanadate.

Kineticsi

  1. KM=3.77 µM for MBP1 Publication
  2. KM=0.62 µM for EF-Tu1 Publication

Vmax=120.48 pmol/min/µg enzyme toward MBP1 Publication

Vmax=2.94 pmol/min/µg enzyme toward EF-Tu1 Publication

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Metal bindingi36 – 361Manganese 1By similarity
Metal bindingi36 – 361Manganese 2By similarity
Metal bindingi37 – 371Manganese 1; via carbonyl oxygenBy similarity
Metal bindingi194 – 1941Manganese 2By similarity
Metal bindingi233 – 2331Manganese 2By similarity

GO - Molecular functioni

  1. metal ion binding Source: UniProtKB-KW
  2. phosphoprotein phosphatase activity Source: UniProtKB-KW
Complete GO annotation...

Keywords - Molecular functioni

Hydrolase, Protein phosphatase

Keywords - Ligandi

Manganese, Metal-binding

Enzyme and pathway databases

BioCyciLMON169963:LMO1821-MONOMER.

Protein family/group databases

PptaseDBiP3D0611176.

Names & Taxonomyi

Protein namesi
Recommended name:
Serine/threonine phosphatase stp (EC:3.1.3.16)
Gene namesi
Name:stp
Ordered Locus Names:lmo1821
OrganismiListeria monocytogenes serovar 1/2a (strain ATCC BAA-679 / EGD-e)
Taxonomic identifieri169963 [NCBI]
Taxonomic lineageiBacteriaFirmicutesBacilliBacillalesListeriaceaeListeria
ProteomesiUP000000817: Chromosome

Organism-specific databases

GenoListiLMO1821.

Subcellular locationi

Cytoplasm 1 Publication. Membrane 1 Publication; Peripheral membrane protein 1 Publication

GO - Cellular componenti

  1. cytoplasm Source: UniProtKB-KW
  2. membrane Source: UniProtKB-KW
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm, Membrane

Pathology & Biotechi

Disruption phenotypei

The stp-disrupted strain is 4 times less virulent than the wild strain, demonstrating that stp is required for full virulence of L.monocytogenes in BALB/c mice.1 Publication

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 252252Serine/threonine phosphatase stpPRO_0000363063Add
BLAST

Interactioni

Protein-protein interaction databases

STRINGi169963.lmo1821.

Structurei

3D structure databases

ProteinModelPortaliQ8Y678.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini2 – 235234PP2C-likeAdd
BLAST

Sequence similaritiesi

Belongs to the PP2C family.Curated
Contains 1 PP2C-like domain.Curated

Phylogenomic databases

eggNOGiCOG0631.
HOGENOMiHOG000235782.
KOiK01090.
OMAiHEECQGM.
OrthoDBiEOG65N17S.
PhylomeDBiQ8Y678.

Family and domain databases

Gene3Di3.60.40.10. 1 hit.
InterProiIPR001932. PP2C-like_dom.
IPR015655. Protein_Pase_2C.
[Graphical view]
PANTHERiPTHR13832. PTHR13832. 1 hit.
PfamiPF00481. PP2C. 1 hit.
[Graphical view]
SMARTiSM00331. PP2C_SIG. 1 hit.
SM00332. PP2Cc. 1 hit.
[Graphical view]
SUPFAMiSSF81606. SSF81606. 1 hit.

Sequencei

Sequence statusi: Complete.

Q8Y678-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MHAEFRTDRG RIRHHNEDNG GVFENKDNQP IVIVADGMGG HRAGDVASEM
60 70 80 90 100
AVRLLSDAWK ETTALLTAEE IETWLRKTIQ EVNKEIVLYA ESEMDLNGMG
110 120 130 140 150
TTLVAAIMAQ SQVVIANVGD SRGYLLQNHV LRQLTEDHSL VHELLRTGEI
160 170 180 190 200
SKEDAMNHPR KNILLRALGV EGKVEVDTFV VPFQTSDTLL LCSDGLTNMV
210 220 230 240 250
PETEMEEILK SKRTLSEKAD VFITKANSYG GEDNITVLLV ERDLTQKGRD

AS
Length:252
Mass (Da):28,026
Last modified:March 1, 2002 - v1
Checksum:i47EE5DA232A43338
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AL591981 Genomic DNA. Translation: CAC99899.1.
PIRiAE1302.
RefSeqiNP_465346.1. NC_003210.1.

Genome annotation databases

EnsemblBacteriaiCAC99899; CAC99899; CAC99899.
GeneIDi985901.
KEGGilmo:lmo1821.
PATRICi20312835. VBILisMon69206_1866.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AL591981 Genomic DNA. Translation: CAC99899.1 .
PIRi AE1302.
RefSeqi NP_465346.1. NC_003210.1.

3D structure databases

ProteinModelPortali Q8Y678.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

STRINGi 169963.lmo1821.

Protein family/group databases

PptaseDBi P3D0611176.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblBacteriai CAC99899 ; CAC99899 ; CAC99899 .
GeneIDi 985901.
KEGGi lmo:lmo1821.
PATRICi 20312835. VBILisMon69206_1866.

Organism-specific databases

GenoListi LMO1821.

Phylogenomic databases

eggNOGi COG0631.
HOGENOMi HOG000235782.
KOi K01090.
OMAi HEECQGM.
OrthoDBi EOG65N17S.
PhylomeDBi Q8Y678.

Enzyme and pathway databases

BioCyci LMON169963:LMO1821-MONOMER.

Family and domain databases

Gene3Di 3.60.40.10. 1 hit.
InterProi IPR001932. PP2C-like_dom.
IPR015655. Protein_Pase_2C.
[Graphical view ]
PANTHERi PTHR13832. PTHR13832. 1 hit.
Pfami PF00481. PP2C. 1 hit.
[Graphical view ]
SMARTi SM00331. PP2C_SIG. 1 hit.
SM00332. PP2Cc. 1 hit.
[Graphical view ]
SUPFAMi SSF81606. SSF81606. 1 hit.
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: ATCC BAA-679 / EGD-e.
  2. "Translation elongation factor EF-Tu is a target for Stp, a serine-threonine phosphatase involved in virulence of Listeria monocytogenes."
    Archambaud C., Gouin E., Pizarro-Cerda J., Cossart P., Dussurget O.
    Mol. Microbiol. 56:383-396(2005) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION AS PHOSPHATASE, SUBCELLULAR LOCATION, COFACTOR, BIOPHYSICOCHEMICAL PROPERTIES, DISRUPTION PHENOTYPE, IDENTIFICATION OF SUBSTRATE.
    Strain: ATCC BAA-679 / EGD-e.

Entry informationi

Entry nameiSTP1_LISMO
AccessioniPrimary (citable) accession number: Q8Y678
Entry historyi
Integrated into UniProtKB/Swiss-Prot: February 10, 2009
Last sequence update: March 1, 2002
Last modified: November 26, 2014
This is version 74 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3