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Protein

Thymidylate synthase

Gene

thyA

Organism
Listeria monocytogenes serovar 1/2a (strain ATCC BAA-679 / EGD-e)
Status
Reviewed-Annotation score: -Protein inferred from homologyi

Functioni

Catalyzes the reductive methylation of 2'-deoxyuridine-5'-monophosphate (dUMP) to 2'-deoxythymidine-5'-monophosphate (dTMP) while utilizing 5,10-methylenetetrahydrofolate (mTHF) as the methyl donor and reductant in the reaction, yielding dihydrofolate (DHF) as a by-product. This enzymatic reaction provides an intracellular de novo source of dTMP, an essential precursor for DNA biosynthesis.UniRule annotation

Catalytic activityi

5,10-methylenetetrahydrofolate + dUMP = dihydrofolate + dTMP.UniRule annotation

Pathwayi: dTTP biosynthesis

This protein is involved in the pathway dTTP biosynthesis, which is part of Pyrimidine metabolism.UniRule annotation
View all proteins of this organism that are known to be involved in the pathway dTTP biosynthesis and in Pyrimidine metabolism.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei21dUMPUniRule annotation1
Active sitei196NucleophileUniRule annotation1
Binding sitei2195,10-methylenetetrahydrofolateUniRule annotation1
Binding sitei227dUMPUniRule annotation1
Binding sitei3135,10-methylenetetrahydrofolate; via carbonyl oxygenUniRule annotation1

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi176 – 177dUMP; shared with dimeric partnerUniRule annotation2
Nucleotide bindingi216 – 219dUMPUniRule annotation4
Nucleotide bindingi257 – 259dUMPUniRule annotation3

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionMethyltransferase, Transferase
Biological processNucleotide biosynthesis

Enzyme and pathway databases

BioCyciLMON169963:G1GTA-2004-MONOMER
UniPathwayiUPA00575

Names & Taxonomyi

Protein namesi
Recommended name:
Thymidylate synthaseUniRule annotation (EC:2.1.1.45UniRule annotation)
Short name:
TSUniRule annotation
Short name:
TSaseUniRule annotation
Gene namesi
Name:thyAUniRule annotation
Ordered Locus Names:lmo1874
OrganismiListeria monocytogenes serovar 1/2a (strain ATCC BAA-679 / EGD-e)
Taxonomic identifieri169963 [NCBI]
Taxonomic lineageiBacteriaFirmicutesBacilliBacillalesListeriaceaeListeria
Proteomesi
  • UP000000817 Componenti: Chromosome

Subcellular locationi

  • Cytoplasm UniRule annotation

GO - Cellular componenti

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001409771 – 314Thymidylate synthaseAdd BLAST314

Proteomic databases

PaxDbiQ8Y626
PRIDEiQ8Y626

Interactioni

Subunit structurei

Homodimer.UniRule annotation

Protein-protein interaction databases

STRINGi169963.lmo1874

Structurei

3D structure databases

ProteinModelPortaliQ8Y626
SMRiQ8Y626
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the thymidylate synthase family. Bacterial-type ThyA subfamily.UniRule annotation

Phylogenomic databases

eggNOGiENOG4105C0V Bacteria
COG0207 LUCA
HOGENOMiHOG000257899
KOiK00560
OMAiKQYLDLC
PhylomeDBiQ8Y626

Family and domain databases

CDDicd00351 TS_Pyrimidine_HMase, 1 hit
Gene3Di3.30.572.10, 1 hit
HAMAPiMF_00008 Thymidy_synth_bact, 1 hit
InterProiView protein in InterPro
IPR023451 Thymidate_synth/dCMP_Mease
IPR036926 Thymidate_synth/dCMP_Mease_sf
IPR000398 Thymidylate_synthase
IPR020940 Thymidylate_synthase_AS
PfamiView protein in Pfam
PF00303 Thymidylat_synt, 1 hit
PRINTSiPR00108 THYMDSNTHASE
SUPFAMiSSF55831 SSF55831, 1 hit
TIGRFAMsiTIGR03284 thym_sym, 1 hit
PROSITEiView protein in PROSITE
PS00091 THYMIDYLATE_SYNTHASE, 1 hit

Sequencei

Sequence statusi: Complete.

Q8Y626-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MKQYLDLEKY VLENGTQKGD RTGTGTISTF GYQMRFDLQE GFPIMTTKRV
60 70 80 90 100
PFKLVVSELL WFLHGDTNIR YLLQHNNNIW NEWAFERFVK SDDYKGEDMT
110 120 130 140 150
DFGLRAERDP AFKEVYQAEM EKFKTRIIED EAFATKYGEL GNIYGKQWRE
160 170 180 190 200
WKTSQGETID QLADLIEMIK TNPNSRRLIV SAWNPEDIPN MALPPCHSLF
210 220 230 240 250
QFYVADGKLS CQLYQRSADI FLGVPFNIAS YALLTHLIAR EVGLDVGEFI
260 270 280 290 300
HTMGDAHLYN NHIEQVKEQL SRTPHALPKL VLSDKPATIF DFEVADISLD
310
GYNPDPSIKA PISV
Length:314
Mass (Da):36,163
Last modified:March 1, 2002 - v1
Checksum:i2E8191ABD21ADF15
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AL591981 Genomic DNA Translation: CAC99952.1
PIRiAB1309
RefSeqiNP_465399.1, NC_003210.1
WP_010989834.1, NC_003210.1

Genome annotation databases

EnsemblBacteriaiCAC99952; CAC99952; CAC99952
GeneIDi985817
KEGGilmo:lmo1874
PATRICifig|169963.11.peg.1919

Similar proteinsi

Entry informationi

Entry nameiTYSY_LISMO
AccessioniPrimary (citable) accession number: Q8Y626
Entry historyiIntegrated into UniProtKB/Swiss-Prot: August 2, 2002
Last sequence update: March 1, 2002
Last modified: May 23, 2018
This is version 95 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome
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Main funding by: National Institutes of Health