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Q8Y5N8

- SYI_LISMO

UniProt

Q8Y5N8 - SYI_LISMO

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Protein

Isoleucine--tRNA ligase

Gene

ileS

Organism
Listeria monocytogenes serovar 1/2a (strain ATCC BAA-679 / EGD-e)
Status
Reviewed - Annotation score: 3 out of 5- Protein inferred from homologyi

Functioni

Catalyzes the attachment of isoleucine to tRNA(Ile). As IleRS can inadvertently accommodate and process structurally similar amino acids such as valine, to avoid such errors it has two additional distinct tRNA(Ile)-dependent editing activities. One activity is designated as 'pretransfer' editing and involves the hydrolysis of activated Val-AMP. The other activity is designated 'posttransfer' editing and involves deacylation of mischarged Val-tRNA(Ile).UniRule annotation

Catalytic activityi

ATP + L-isoleucine + tRNA(Ile) = AMP + diphosphate + L-isoleucyl-tRNA(Ile).UniRule annotation

Cofactori

Binds 1 zinc ion per subunit.UniRule annotation

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei552 – 5521Aminoacyl-adenylateUniRule annotation
Binding sitei596 – 5961ATPUniRule annotation
Metal bindingi888 – 8881ZincUniRule annotation
Metal bindingi891 – 8911ZincUniRule annotation
Metal bindingi908 – 9081ZincUniRule annotation
Metal bindingi911 – 9111ZincUniRule annotation

GO - Molecular functioni

  1. aminoacyl-tRNA editing activity Source: InterPro
  2. ATP binding Source: UniProtKB-HAMAP
  3. isoleucine-tRNA ligase activity Source: UniProtKB-HAMAP
  4. zinc ion binding Source: UniProtKB-HAMAP

GO - Biological processi

  1. isoleucyl-tRNA aminoacylation Source: UniProtKB-HAMAP
Complete GO annotation...

Keywords - Molecular functioni

Aminoacyl-tRNA synthetase, Ligase

Keywords - Biological processi

Protein biosynthesis

Keywords - Ligandi

ATP-binding, Metal-binding, Nucleotide-binding, Zinc

Enzyme and pathway databases

BioCyciLMON169963:LMO2019-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
Isoleucine--tRNA ligaseUniRule annotation (EC:6.1.1.5UniRule annotation)
Alternative name(s):
Isoleucyl-tRNA synthetaseUniRule annotation
Short name:
IleRSUniRule annotation
Gene namesi
Name:ileSUniRule annotation
Ordered Locus Names:lmo2019
OrganismiListeria monocytogenes serovar 1/2a (strain ATCC BAA-679 / EGD-e)
Taxonomic identifieri169963 [NCBI]
Taxonomic lineageiBacteriaFirmicutesBacilliBacillalesListeriaceaeListeria
ProteomesiUP000000817: Chromosome

Organism-specific databases

GenoListiLMO2019.

Subcellular locationi

Cytoplasm UniRule annotation

GO - Cellular componenti

  1. cytoplasm Source: UniProtKB-HAMAP
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 921921Isoleucine--tRNA ligasePRO_0000098411Add
BLAST

PTM databases

PhosSiteiP11121157.

Interactioni

Subunit structurei

Monomer.UniRule annotation

Protein-protein interaction databases

STRINGi169963.lmo2019.

Structurei

3D structure databases

ProteinModelPortaliQ8Y5N8.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Motif

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Motifi57 – 6711"HIGH" regionAdd
BLAST
Motifi593 – 5975"KMSKS" region

Domaini

IleRS has two distinct active sites: one for aminoacylation and one for editing. The misactivated valine is translocated from the active site to the editing site, which sterically excludes the correctly activated isoleucine. The single editing site contains two valyl binding pockets, one specific for each substrate (Val-AMP or Val-tRNA(Ile)).UniRule annotation

Sequence similaritiesi

Belongs to the class-I aminoacyl-tRNA synthetase family. IleS type 1 subfamily.UniRule annotation

Phylogenomic databases

eggNOGiCOG0060.
HOGENOMiHOG000246402.
KOiK01870.
OMAiKPVHWCL.
OrthoDBiEOG644ZM1.
PhylomeDBiQ8Y5N8.

Family and domain databases

Gene3Di1.10.730.10. 1 hit.
3.40.50.620. 2 hits.
3.90.740.10. 1 hit.
HAMAPiMF_02002. Ile_tRNA_synth_type1.
InterProiIPR001412. aa-tRNA-synth_I_CS.
IPR002300. aa-tRNA-synth_Ia.
IPR002301. Ile-tRNA-ligase.
IPR023585. Ile-tRNA-ligase_type1.
IPR014729. Rossmann-like_a/b/a_fold.
IPR009080. tRNAsynth_1a_anticodon-bd.
IPR013155. V/L/I-tRNA-synth_anticodon-bd.
IPR009008. Val/Leu/Ile-tRNA-synth_edit.
IPR010663. Znf_DNA_glyclase/IsotRNA_synth.
[Graphical view]
PANTHERiPTHR11946:SF9. PTHR11946:SF9. 1 hit.
PfamiPF08264. Anticodon_1. 1 hit.
PF00133. tRNA-synt_1. 1 hit.
PF06827. zf-FPG_IleRS. 1 hit.
[Graphical view]
PRINTSiPR00984. TRNASYNTHILE.
SUPFAMiSSF47323. SSF47323. 1 hit.
SSF50677. SSF50677. 1 hit.
TIGRFAMsiTIGR00392. ileS. 1 hit.
PROSITEiPS00178. AA_TRNA_LIGASE_I. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q8Y5N8 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MEYKDTLLMP KTDFPMRGNL PNKEPEWQAK WEEEKLYEKI QEKNAGRPTY
60 70 80 90 100
ILHDGPPYAN GELHMGHALN KTIKDIIVRY KSMAGFSSPY VPGWDTHGLP
110 120 130 140 150
IETAIAKKGV KRKEMSIAEF RKLCAEYAMK QVDGQRTGFK RLGINGDWEN
160 170 180 190 200
PYITLLPEYE AEQIKVFGEM AKKGYIYKGK KPVYWSPSSE SALAEAEIEY
210 220 230 240 250
QDKTSASIFV AFKVTDGKGV LDEGTNIVIW TTTPWTIPAN MGITVNPDLD
260 270 280 290 300
YVVIESAGEK YVVAEALLPS LREKLGFEDA TVVKTVRGSE LDRVVTKHPF
310 320 330 340 350
YDRDSLVMNG EHATAEAGTG AVHTAPGHGE DDFLIGKKYD LEILAPLDDR
360 370 380 390 400
GVFTEEAPGF EGVFYDTANK MVTEKLEEVG ALLKMEFITH SYPHDWRTKK
410 420 430 440 450
PVIFRATAQW FASIDAFRDD LLAAVKGVNW TPAWGETRLF NMVRDRGDWV
460 470 480 490 500
ISRQRAWGVP LPIFYAENGE AIITDETINH ISELFREHGS NVWFERDVKD
510 520 530 540 550
LLPAGFTHPG SPNGEFTKET DIMDVWFDSG SSHQAVLNAR PELSRPADLY
560 570 580 590 600
MEGSDQYRGW FNSSLTTAVA ITGEAPYRNV LSHGFALDGE GRKMSKSLGN
610 620 630 640 650
TLLPGKVIKQ LGADIVRLWV ASVDYQADVR VSDEILKQVS EVYRKIRNTM
660 670 680 690 700
RFLLGNINDF NPTTNTVSYE NLREVDKYML IKLNDLVKNV KDSYEAFEFS
710 720 730 740 750
TIYHQINNFC TVELSQFYMD FAKDVVYIEA ADSHDRRAMQ TVFYEAVVTL
760 770 780 790 800
TKLLAPILPH TTEEVWNSLI GEGAESIHLQ DLPDVKVLAN SEEITAKWDA
810 820 830 840 850
FMQIRDNVQK ALEFARNEKL IGKSMLAKVT LYVDGEAKTL FDSLEGDFAQ
860 870 880 890 900
LFIVSDFELV EGLENAPESA FKSNQVAVQI TVAEGETCER CRVVKKDVGV
910 920
NPKHPTLCGR CADIVVKHYE A
Length:921
Mass (Da):103,851
Last modified:March 1, 2002 - v1
Checksum:iA1FB8ED2EB475471
GO

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AL591981 Genomic DNA. Translation: CAD00097.1.
PIRiAC1327.
RefSeqiNP_465543.1. NC_003210.1.

Genome annotation databases

EnsemblBacteriaiCAD00097; CAD00097; CAD00097.
GeneIDi986874.
KEGGilmo:lmo2019.
PATRICi20313237. VBILisMon69206_2067.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AL591981 Genomic DNA. Translation: CAD00097.1 .
PIRi AC1327.
RefSeqi NP_465543.1. NC_003210.1.

3D structure databases

ProteinModelPortali Q8Y5N8.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

STRINGi 169963.lmo2019.

PTM databases

PhosSitei P11121157.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblBacteriai CAD00097 ; CAD00097 ; CAD00097 .
GeneIDi 986874.
KEGGi lmo:lmo2019.
PATRICi 20313237. VBILisMon69206_2067.

Organism-specific databases

GenoListi LMO2019.

Phylogenomic databases

eggNOGi COG0060.
HOGENOMi HOG000246402.
KOi K01870.
OMAi KPVHWCL.
OrthoDBi EOG644ZM1.
PhylomeDBi Q8Y5N8.

Enzyme and pathway databases

BioCyci LMON169963:LMO2019-MONOMER.

Family and domain databases

Gene3Di 1.10.730.10. 1 hit.
3.40.50.620. 2 hits.
3.90.740.10. 1 hit.
HAMAPi MF_02002. Ile_tRNA_synth_type1.
InterProi IPR001412. aa-tRNA-synth_I_CS.
IPR002300. aa-tRNA-synth_Ia.
IPR002301. Ile-tRNA-ligase.
IPR023585. Ile-tRNA-ligase_type1.
IPR014729. Rossmann-like_a/b/a_fold.
IPR009080. tRNAsynth_1a_anticodon-bd.
IPR013155. V/L/I-tRNA-synth_anticodon-bd.
IPR009008. Val/Leu/Ile-tRNA-synth_edit.
IPR010663. Znf_DNA_glyclase/IsotRNA_synth.
[Graphical view ]
PANTHERi PTHR11946:SF9. PTHR11946:SF9. 1 hit.
Pfami PF08264. Anticodon_1. 1 hit.
PF00133. tRNA-synt_1. 1 hit.
PF06827. zf-FPG_IleRS. 1 hit.
[Graphical view ]
PRINTSi PR00984. TRNASYNTHILE.
SUPFAMi SSF47323. SSF47323. 1 hit.
SSF50677. SSF50677. 1 hit.
TIGRFAMsi TIGR00392. ileS. 1 hit.
PROSITEi PS00178. AA_TRNA_LIGASE_I. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

  1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: ATCC BAA-679 / EGD-e.

Entry informationi

Entry nameiSYI_LISMO
AccessioniPrimary (citable) accession number: Q8Y5N8
Entry historyi
Integrated into UniProtKB/Swiss-Prot: December 20, 2005
Last sequence update: March 1, 2002
Last modified: October 29, 2014
This is version 84 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Aminoacyl-tRNA synthetases
    List of aminoacyl-tRNA synthetase entries
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3