Q8Y5N4 (NADB_LISMO) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
L-aspartate oxidase
Short name=LASPO
EC=1.4.3.16

Alternative name(s):
Quinolinate synthase B

Gene names

Name:	nadB
Ordered Locus Names:	lmo2023

Organism

Listeria monocytogenes

Taxonomic identifier

1639 [NCBI]

Taxonomic lineage

Bacteria › Firmicutes › Bacillales › Listeriaceae › Listeria

Protein attributes

Sequence length	484 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Catalyzes the oxidation of L-aspartate to iminoaspartate.
Catalytic activity	L-aspartate + O2 = iminosuccinate + H2O2.
Cofactor	FAD.
Pathway	Cofactor biosynthesis; NAD(+) biosynthesis; iminoaspartate from L-aspartate (oxidase route): step 1/1.
Subcellular location	Cytoplasm By similarity.
Sequence similarities	Belongs to the FAD-dependent oxidoreductase 2 family. NadB subfamily.

Ontologies

Keywords
Biological process	Pyridine nucleotide biosynthesis
Cellular component	Cytoplasm
Ligand	FAD Flavoprotein
Molecular function	Oxidoreductase
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological process	NAD biosynthetic process Inferred from electronic annotation. Source: InterPro
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	L-aspartate oxidase activity Inferred from electronic annotation. Source: EC L-aspartate:fumarate oxidoreductase activity Inferred from electronic annotation. Source: EC electron carrier activity Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 484	484	L-aspartate oxidase		PRO_0000184387
Regions
	Nucleotide binding	7 – 21	15	FAD Potential
Sites
	Active site	224	1	By similarity
	Active site	243	1	By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q8Y5N4 [UniParc]. Last modified March 1, 2002. Version 1. Checksum: 304FDD719D443957	FASTA	484	53,115
	10 20 30 40 50 60 MIKERVIIVG SGISGCTAAL RLMQDYDVTI ITKGYKEESN SMLAQGGVAA AVSKNDTPKK 70 80 90 100 110 120 HFSDTFQAGC FHNKVLAVNQ LVTHGPMVIQ KLIAEGMAFD EQDGELALGL EGAHQLPRIL 130 140 150 160 170 180 HTGGDQTGKF LTTFLQEKLT DIHWQEQKMA IEIIKQNDSA IGVHCLDKEN RLHTYYGEHI 190 200 210 220 230 240 ILASGGLGQL FPVTTNAATI SGDGLALAYR AGAKLTDMEF IQFHPTLLFL NGRCHGLISE 250 260 270 280 290 300 AVRGEGAKLI RADGSAIMTD VHPRADLAPR DIVAATLFAE IQDGNEVFLD ITAIPNFEKR 310 320 330 340 350 360 FPGITANLDA HHIPFRETKR IPVHPGAHFL MGGIRTDLSG KTNIPGLYAI GEVANAGVHG 370 380 390 400 410 420 ANRLASNSLL ETLVFGEKVA EYIRTQKINP IDHPEIPLSN QIQTPHLPDK QLLQEKIWET 430 440 450 460 470 480 LGITRKPEKI TEFLHWLTDF DYANHTRKTA EISHMLITAK LIAESALKRT ESLGAHRILK GVIK

References

[1]

"Comparative genomics of Listeria species." Glaser P., Frangeul L., Buchrieser C., Rusniok C., Amend A., Baquero F., Berche P., Bloecker H., Brandt P., Chakraborty T., Charbit A., Chetouani F., Couve E., de Daruvar A., Dehoux P., Domann E., Dominguez-Bernal G., Duchaud E. , Durant L., Dussurget O., Entian K.-D., Fsihi H., Garcia-del Portillo F., Garrido P., Gautier L., Goebel W., Gomez-Lopez N., Hain T., Hauf J., Jackson D., Jones L.-M., Kaerst U., Kreft J., Kuhn M., Kunst F., Kurapkat G., Madueno E., Maitournam A., Mata Vicente J., Ng E., Nedjari H., Nordsiek G., Novella S., de Pablos B., Perez-Diaz J.-C., Purcell R., Remmel B., Rose M., Schlueter T., Simoes N., Tierrez A., Vazquez-Boland J.-A., Voss H., Wehland J., Cossart P. Science 294:849-852(2001) [PubMed: 11679669] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC BAA-679 / EGD-e / Serovar 1/2a.

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Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AL591982 Genomic DNA. Translation: CAD00101.1.
PIR	AG1327.
RefSeq	NP_465547.1. NC_003210.1.
3D structure databases
ProteinModelPortal	Q8Y5N4.
ModBase	Search...
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	986699.
GenomeReviews	Gene locus lmo2023 in contig AL591824_GR.
KEGG	lmo:lmo2023.
PATRIC	20313245. VBILisMon69206_2071.
Organism-specific databases
GenoList	LMO2023.
Phylogenomic databases
HOGENOM	HBG293998.
OMA	GAYIWNR.
ProtClustDB	PRK08071.
Enzyme and pathway databases
BioCyc	LMON169963:LMO2023-MONOMER.
Family and domain databases
InterPro	IPR003953. FAD_bind2_N. IPR013027. FAD_pyr_nucl-diS_OxRdtase. IPR015939. Fum_Rdtase/Succ_DH_flav-like_C. IPR004112. Fum_Rdtase/Succ_DH_flav_C. IPR005288. NadB. [Graphical view]
Gene3D	G3DSA:1.20.58.100. Fum_Rdtase/Succ_DH_flav-like_C. 1 hit.
KO	K00278.
Pfam	PF00890. FAD_binding_2. 1 hit. PF02910. Succ_DH_flav_C. 1 hit. [Graphical view]
PRINTS	PR00368. FADPNR.
SUPFAM	SSF46977. Succ_DH_flav_C. 1 hit.
TIGRFAMs	TIGR00551. NadB. 1 hit.
ProtoNet	Search...

Entry information

Entry name

NADB_LISMO

Accession

Primary (citable) accession number: Q8Y5N4

Entry history

Integrated into UniProtKB/Swiss-Prot:	August 30, 2002
Last sequence update:	March 1, 2002
Last modified:	January 25, 2012
This is version 58 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program