Q8Y5J6 (MSCL_LISMO) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Large-conductance mechanosensitive channel

Gene names

Name:	mscL
Ordered Locus Names:	lmo2064

Organism

Listeria monocytogenes

Taxonomic identifier

1639 [NCBI]

Taxonomic lineage

Bacteria › Firmicutes › Bacillales › Listeriaceae › Listeria

Protein attributes

Sequence length	128 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Channel that opens in response to stretch forces in the membrane lipid bilayer. May participate in the regulation of osmotic pressure changes within the cell By similarity. HAMAP MF_00115
Subunit structure	Homopentamer By similarity. HAMAP MF_00115
Subcellular location	Cell membrane; Multi-pass membrane protein By similarity HAMAP MF_00115.
Sequence similarities	Belongs to the MscL family.

Ontologies

Keywords
Biological process	Ion transport Transport
Cellular component	Cell membrane Membrane
Domain	Transmembrane Transmembrane helix
Molecular function	Ionic channel
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Cellular component	integral to membrane Inferred from electronic annotation. Source: UniProtKB-KW plasma membrane Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	ion channel activity Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 128	128	Large-conductance mechanosensitive channel HAMAP MF_00115		PRO_0000192449
Regions
	Topological domain	1 – 10	10	Cytoplasmic Potential
	Transmembrane	11 – 31	21	Helical; Potential
	Topological domain	32 – 69	38	Extracellular Potential
	Transmembrane	70 – 90	21	Helical; Potential
	Topological domain	91 – 128	38	Cytoplasmic Potential

Sequences

	Sequence		Length	Mass (Da)
	Q8Y5J6 [UniParc]. Last modified March 1, 2002. Version 1. Checksum: 87CB121823326806	FASTA	128	14,239
	10 20 30 40 50 60 MKKMLVEFRD FALKGNVLDL AVAVVIGAAF GKIVSSLVDN IIMPLVGVLL GGLDFTDLSF 70 80 90 100 110 120 KVGKSVIQYG AFIQSIVDFI IIAFAIFIFV KVLTSFIKKK EQTVEETPVP PTEEYLKEIR DLLKEQQK

References

[1]

"Comparative genomics of Listeria species." Glaser P., Frangeul L., Buchrieser C., Rusniok C., Amend A., Baquero F., Berche P., Bloecker H., Brandt P., Chakraborty T., Charbit A., Chetouani F., Couve E., de Daruvar A., Dehoux P., Domann E., Dominguez-Bernal G., Duchaud E. , Durant L., Dussurget O., Entian K.-D., Fsihi H., Garcia-del Portillo F., Garrido P., Gautier L., Goebel W., Gomez-Lopez N., Hain T., Hauf J., Jackson D., Jones L.-M., Kaerst U., Kreft J., Kuhn M., Kunst F., Kurapkat G., Madueno E., Maitournam A., Mata Vicente J., Ng E., Nedjari H., Nordsiek G., Novella S., de Pablos B., Perez-Diaz J.-C., Purcell R., Remmel B., Rose M., Schlueter T., Simoes N., Tierrez A., Vazquez-Boland J.-A., Voss H., Wehland J., Cossart P. Science 294:849-852(2001) [PubMed: 11679669] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC BAA-679 / EGD-e / Serovar 1/2a.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AL591982 Genomic DNA. Translation: CAD00142.1.
PIR	AH1332.
RefSeq	NP_465588.1. NC_003210.1.
3D structure databases
ProteinModelPortal	Q8Y5J6.
ModBase	Search...
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	984800.
GenomeReviews	Gene locus lmo2064 in contig AL591824_GR.
KEGG	lmo:lmo2064.
PATRIC	20313328. VBILisMon69206_2112.
Organism-specific databases
GenoList	LMO2064.
Phylogenomic databases
HOGENOM	HBG727604.
OMA	VIDFVIV.
ProtClustDB	PRK13953.
Enzyme and pathway databases
BioCyc	LMON169963:LMO2064-MONOMER.
Family and domain databases
HAMAP	MF_00115. MscL. [Tree]
InterPro	IPR019823. Mechanosensitive_channel_CS. IPR001185. MS_channel. [Graphical view]
KO	K03282.
Pfam	PF01741. MscL. 1 hit. [Graphical view]
PRINTS	PR01264. MECHCHANNEL.
SUPFAM	SSF81330. MS_channel. 1 hit.
TIGRFAMs	TIGR00220. MscL. 1 hit.
PROSITE	PS01327. MSCL. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

MSCL_LISMO

Accession

Primary (citable) accession number: Q8Y5J6

Entry history

Integrated into UniProtKB/Swiss-Prot:	October 31, 2003
Last sequence update:	March 1, 2002
Last modified:	January 25, 2012
This is version 58 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program