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Q8Y5H2 (ASSY_LISMO) Reviewed, UniProtKB/Swiss-Prot

Last modified July 9, 2014. Version 84. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Argininosuccinate synthase

EC=6.3.4.5
Alternative name(s):
Citrulline--aspartate ligase
Gene names
Name:argG
Ordered Locus Names:lmo2090
OrganismListeria monocytogenes serovar 1/2a (strain ATCC BAA-679 / EGD-e) [Reference proteome] [HAMAP]
Taxonomic identifier169963 [NCBI]
Taxonomic lineageBacteriaFirmicutesBacilliBacillalesListeriaceaeListeria

Protein attributes

Sequence length404 AA.
Sequence statusComplete.
Protein existenceInferred from homology

General annotation (Comments)

Catalytic activity

ATP + L-citrulline + L-aspartate = AMP + diphosphate + N(omega)-(L-arginino)succinate. HAMAP-Rule MF_00005

Pathway

Amino-acid biosynthesis; L-arginine biosynthesis; L-arginine from L-ornithine and carbamoyl phosphate: step 2/3. HAMAP-Rule MF_00005

Subunit structure

Homotetramer By similarity. HAMAP-Rule MF_00005

Subcellular location

Cytoplasm By similarity HAMAP-Rule MF_00005.

Sequence similarities

Belongs to the argininosuccinate synthase family. Type 1 subfamily.

Ontologies

Keywords
   Biological processAmino-acid biosynthesis
Arginine biosynthesis
   Cellular componentCytoplasm
   LigandATP-binding
Nucleotide-binding
   Molecular functionLigase
   Technical termComplete proteome
Reference proteome
Gene Ontology (GO)
   Biological_processarginine biosynthetic process

Inferred from electronic annotation. Source: UniProtKB-HAMAP

   Cellular_componentcytoplasm

Inferred from electronic annotation. Source: UniProtKB-SubCell

   Molecular_functionATP binding

Inferred from electronic annotation. Source: UniProtKB-HAMAP

argininosuccinate synthase activity

Inferred from electronic annotation. Source: UniProtKB-HAMAP

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 404404Argininosuccinate synthase HAMAP-Rule MF_00005
PRO_0000148610

Regions

Nucleotide binding9 – 179ATP By similarity

Sites

Binding site861Citrulline By similarity
Binding site1161ATP; via amide nitrogen By similarity
Binding site1181Aspartate By similarity
Binding site1221Aspartate By similarity
Binding site1221Citrulline By similarity
Binding site1231Aspartate By similarity
Binding site1261Citrulline By similarity
Binding site1741Citrulline By similarity
Binding site1831Citrulline By similarity
Binding site2591Citrulline By similarity
Binding site2711Citrulline By similarity

Sequences

Sequence LengthMass (Da)Tools
Q8Y5H2 [UniParc].

Last modified March 1, 2002. Version 1.
Checksum: 2C150FFC1B3BF4C3

FASTA40444,480
        10         20         30         40         50         60 
MAKEKIVLAY SGGLDTSVAI QWLVESGYEV IACCLDVGEG KNLDFIKEKA ITVGASESYT 

        70         80         90        100        110        120 
IDAKEEFAED FALIALQAHA YYEGKYPLIS ALSRPLIAKK LVEVARQEGA SAIAHGCTGK 

       130        140        150        160        170        180 
GNDQVRFEVA IHALAPDLKV VSPVRDWKWS REEEINYAKE HDIPVPIDLD NPFSIDQNLW 

       190        200        210        220        230        240 
GRSNECGVLE NPWTTPPEAA YDLTVSLEDA PDTADIVEIT FDAGIPISLN GENMSLANLI 

       250        260        270        280        290        300 
LTLNEIAGKH GVGRIDHIEN RLVGIKSREV YECPAAVTLI TAHKELEDLT FVREVAHFKP 

       310        320        330        340        350        360 
IIEQKISETI YNGLWFSPLT EALVAFLKST QKFVNGTIRV KLFKGHAIVE GRKSPNSLYD 

       370        380        390        400 
ENLATYTSSD TFDQDAAVGF IKLWGLPTKV NAEVNSKVTI TTEV 

« Hide

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
AL591982 Genomic DNA. Translation: CAD00168.1.
PIRAB1336.
RefSeqNP_465614.1. NC_003210.1.

3D structure databases

ProteinModelPortalQ8Y5H2.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

STRING169963.lmo2090.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaCAD00168; CAD00168; CAD00168.
GeneID984368.
KEGGlmo:lmo2090.
PATRIC20313386. VBILisMon69206_2140.

Organism-specific databases

GenoListLMO2090.

Phylogenomic databases

eggNOGCOG0137.
HOGENOMHOG000230093.
KOK01940.
OMAAPPEEAY.
OrthoDBEOG6K9QCV.
PhylomeDBQ8Y5H2.

Enzyme and pathway databases

BioCycLMON169963:LMO2090-MONOMER.
UniPathwayUPA00068; UER00113.

Family and domain databases

Gene3D3.40.50.620. 1 hit.
3.90.1260.10. 1 hit.
HAMAPMF_00005. Arg_succ_synth_type1.
InterProIPR001518. Arginosuc_synth.
IPR018223. Arginosuc_synth_CS.
IPR023434. Arginosuc_synth_type_1_subfam.
IPR024074. AS_cat/multimer_dom_body.
IPR014729. Rossmann-like_a/b/a_fold.
[Graphical view]
PfamPF00764. Arginosuc_synth. 1 hit.
[Graphical view]
TIGRFAMsTIGR00032. argG. 1 hit.
PROSITEPS00564. ARGININOSUCCIN_SYN_1. 1 hit.
PS00565. ARGININOSUCCIN_SYN_2. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameASSY_LISMO
AccessionPrimary (citable) accession number: Q8Y5H2
Entry history
Integrated into UniProtKB/Swiss-Prot: May 27, 2002
Last sequence update: March 1, 2002
Last modified: July 9, 2014
This is version 84 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

PATHWAY comments

Index of metabolic and biosynthesis pathways