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Protein

Diadenylate cyclase

Gene

dacA

Organism
Listeria monocytogenes serovar 1/2a (strain ATCC BAA-679 / EGD-e)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Catalyzes the condensation of 2 ATP molecules into cyclic di-AMP (c-di-AMP) (PubMed:25605729). c-di-AMP is a signaling compound secreted into the host's cytosol where it triggers the cytosolic surveillance pathway (CSP), a host pathway of innate immunity characterized by expression of beta interferon (IFN-beta) and coregulated genes (PubMed:20508090). Expression of truncated proteins (missing first 80 or 100 residues) in E.coli leads to c-di-AMP synthesis (PubMed:25605729).2 Publications

Catalytic activityi

2 ATP = 2 diphosphate + cyclic di-3',5'-adenylate.UniRule annotation

Cofactori

Co2+1 PublicationNote: Optimal activity at 0.5-1 mM CoCl(2), also functions with Mn2+, for a construct missing residues 1-80.1 Publication

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei171ATPCombined sources1
Binding sitei188ATP; via amide nitrogen and carbonyl oxygenCombined sources1

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi201 – 204ATPCombined sources4
Nucleotide bindingi222 – 224ATPCombined sources3

GO - Molecular functioni

  • adenylate cyclase activity Source: UniProtKB
  • ATP binding Source: UniProtKB-KW
  • nucleotidyltransferase activity Source: UniProtKB-KW
Complete GO annotation...

Keywords - Molecular functioni

Nucleotidyltransferase, Transferase

Keywords - Ligandi

ATP-binding, Nucleotide-binding

Enzyme and pathway databases

BRENDAi2.7.7.85. 3045.

Names & Taxonomyi

Protein namesi
Recommended name:
Diadenylate cyclase1 PublicationUniRule annotation (EC:2.7.7.85UniRule annotation)
Short name:
DACUniRule annotation
Alternative name(s):
Cyclic-di-AMP synthaseUniRule annotation
Short name:
c-di-AMP synthaseUniRule annotation
Diadenylate cyclase CdaA1 Publication
Gene namesi
Name:dacA1 PublicationUniRule annotation
Synonyms:cdaA1 Publication
Ordered Locus Names:lmo2120
OrganismiListeria monocytogenes serovar 1/2a (strain ATCC BAA-679 / EGD-e)
Taxonomic identifieri169963 [NCBI]
Taxonomic lineageiBacteriaFirmicutesBacilliBacillalesListeriaceaeListeria
Proteomesi
  • UP000000817 Componenti: Chromosome

Subcellular locationi

  • Cell membrane UniRule annotation; Multi-pass membrane protein UniRule annotation

Topology

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Transmembranei12 – 32HelicalUniRule annotationAdd BLAST21
Transmembranei37 – 57HelicalUniRule annotationAdd BLAST21
Transmembranei61 – 81HelicalUniRule annotationAdd BLAST21

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Cell membrane, Membrane

Pathology & Biotechi

Disruption phenotypei

Essential, it cannot be deleted.1 Publication

Mutagenesis

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Mutagenesisi171D → N: No c-di-AMP formation (construct without residues 1-80). 1 Publication1
Mutagenesisi172G → A: No c-di-AMP formation (construct without residues 1-80). 1 Publication1
Mutagenesisi202T → N: No c-di-AMP formation (construct without residues 1-80). 1 Publication1

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00004241791 – 273Diadenylate cyclaseAdd BLAST273

Proteomic databases

PaxDbiQ8Y5E4.

Interactioni

Subunit structurei

Homodimer, required for active site formation (Probable).1 Publication

Protein-protein interaction databases

STRINGi169963.lmo2120.

Structurei

Secondary structure

1273
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Helixi101 – 126Combined sources26
Beta strandi130 – 134Combined sources5
Beta strandi136 – 139Combined sources4
Helixi141 – 144Combined sources4
Beta strandi147 – 153Combined sources7
Helixi156 – 162Combined sources7
Helixi168 – 170Combined sources3
Beta strandi171 – 177Combined sources7
Beta strandi180 – 186Combined sources7
Helixi202 – 213Combined sources12
Beta strandi217 – 221Combined sources5
Turni223 – 225Combined sources3
Beta strandi228 – 232Combined sources5
Beta strandi235 – 237Combined sources3
Helixi242 – 253Combined sources12

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
4RV7X-ray2.80A/B/C/D101-273[»]
ProteinModelPortaliQ8Y5E4.
SMRiQ8Y5E4.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini82 – 242DACPROSITE-ProRule annotationAdd BLAST161

Sequence similaritiesi

Belongs to the adenylate cyclase family. DacA/CdaA subfamily.UniRule annotation
Contains 1 DAC domain.PROSITE-ProRule annotation

Keywords - Domaini

Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiENOG4105C8B. Bacteria.
COG1624. LUCA.
HOGENOMiHOG000054800.
KOiK18672.
OMAiWRDLLDI.
PhylomeDBiQ8Y5E4.

Family and domain databases

HAMAPiMF_01499. DacA. 1 hit.
InterProiIPR014046. c-di-AMP_synthase.
IPR003390. DNA_integrity_scan_DisA_N.
[Graphical view]
PfamiPF02457. DisA_N. 1 hit.
[Graphical view]
PIRSFiPIRSF004793. UCP004793. 1 hit.
TIGRFAMsiTIGR00159. TIGR00159. 1 hit.
PROSITEiPS51794. DAC. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q8Y5E4-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MDFSNMSILH YLANIVDILV VWFVIYKVIM LIRGTKAVQL LKGIFIIIAV
60 70 80 90 100
KLLSGFFGLQ TVEWITDQML TWGFLAIIII FQPELRRALE TLGRGNIFTR
110 120 130 140 150
YGSRIEREQH HLIESIEKST QYMAKRRIGA LISVARDTGM DDYIETGIPL
160 170 180 190 200
NAKISSQLLI NIFIPNTPLH DGAVIIKGNE IASAASYLPL SDSPFLSKEL
210 220 230 240 250
GTRHRAALGI SEVTDSITIV VSEETGGISL TKGGELFRDV SEEELHKILL
260 270
KELVTVTAKK PSIFSKWKGG KSE
Length:273
Mass (Da):30,445
Last modified:March 1, 2002 - v1
Checksum:i878951A75FB06D88
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AL591982 Genomic DNA. Translation: CAD00198.1.
PIRiAH1339.
RefSeqiNP_465644.1. NC_003210.1.
WP_003722380.1. NC_003210.1.

Genome annotation databases

EnsemblBacteriaiCAD00198; CAD00198; CAD00198.
GeneIDi984739.
KEGGilmo:lmo2120.
PATRICi20313450. VBILisMon69206_2172.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AL591982 Genomic DNA. Translation: CAD00198.1.
PIRiAH1339.
RefSeqiNP_465644.1. NC_003210.1.
WP_003722380.1. NC_003210.1.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
4RV7X-ray2.80A/B/C/D101-273[»]
ProteinModelPortaliQ8Y5E4.
SMRiQ8Y5E4.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi169963.lmo2120.

Proteomic databases

PaxDbiQ8Y5E4.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiCAD00198; CAD00198; CAD00198.
GeneIDi984739.
KEGGilmo:lmo2120.
PATRICi20313450. VBILisMon69206_2172.

Phylogenomic databases

eggNOGiENOG4105C8B. Bacteria.
COG1624. LUCA.
HOGENOMiHOG000054800.
KOiK18672.
OMAiWRDLLDI.
PhylomeDBiQ8Y5E4.

Enzyme and pathway databases

BRENDAi2.7.7.85. 3045.

Family and domain databases

HAMAPiMF_01499. DacA. 1 hit.
InterProiIPR014046. c-di-AMP_synthase.
IPR003390. DNA_integrity_scan_DisA_N.
[Graphical view]
PfamiPF02457. DisA_N. 1 hit.
[Graphical view]
PIRSFiPIRSF004793. UCP004793. 1 hit.
TIGRFAMsiTIGR00159. TIGR00159. 1 hit.
PROSITEiPS51794. DAC. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiDACA_LISMO
AccessioniPrimary (citable) accession number: Q8Y5E4
Entry historyi
Integrated into UniProtKB/Swiss-Prot: October 16, 2013
Last sequence update: March 1, 2002
Last modified: November 2, 2016
This is version 76 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.