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Protein

Diadenylate cyclase

Gene

dacA

Organism
Listeria monocytogenes serovar 1/2a (strain ATCC BAA-679 / EGD-e)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Catalyzes the condensation of 2 ATP molecules into cyclic di-AMP (c-di-AMP) (PubMed:25605729). c-di-AMP is a signaling compound secreted into the host's cytosol where it triggers the cytosolic surveillance pathway (CSP), a host pathway of innate immunity characterized by expression of beta interferon (IFN-beta) and coregulated genes (PubMed:20508090). Expression of truncated proteins (missing first 80 or 100 residues) in E.coli leads to c-di-AMP synthesis (PubMed:25605729).2 Publications

Catalytic activityi

2 ATP = 2 diphosphate + cyclic di-3',5'-adenylate.UniRule annotation

Cofactori

Co2+1 PublicationNote: Optimal activity at 0.5-1 mM CoCl(2), also functions with Mn2+, for a construct missing residues 1-80.1 Publication

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei171 – 1711ATPCombined sources
Binding sitei188 – 1881ATP; via amide nitrogen and carbonyl oxygenCombined sources

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Nucleotide bindingi201 – 2044ATPCombined sources
Nucleotide bindingi222 – 2243ATPCombined sources

GO - Molecular functioni

  • adenylate cyclase activity Source: UniProtKB
  • ATP binding Source: UniProtKB-KW
  • nucleotidyltransferase activity Source: UniProtKB-KW
Complete GO annotation...

Keywords - Molecular functioni

Nucleotidyltransferase, Transferase

Keywords - Ligandi

ATP-binding, Nucleotide-binding

Enzyme and pathway databases

BioCyciLMON169963:LMO2120-MONOMER.
BRENDAi2.7.7.85. 3045.

Names & Taxonomyi

Protein namesi
Recommended name:
Diadenylate cyclase1 PublicationUniRule annotation (EC:2.7.7.85UniRule annotation)
Short name:
DACUniRule annotation
Alternative name(s):
Cyclic-di-AMP synthaseUniRule annotation
Short name:
c-di-AMP synthaseUniRule annotation
Diadenylate cyclase CdaA1 Publication
Gene namesi
Name:dacA1 PublicationUniRule annotation
Synonyms:cdaA1 Publication
Ordered Locus Names:lmo2120
OrganismiListeria monocytogenes serovar 1/2a (strain ATCC BAA-679 / EGD-e)
Taxonomic identifieri169963 [NCBI]
Taxonomic lineageiBacteriaFirmicutesBacilliBacillalesListeriaceaeListeria
Proteomesi
  • UP000000817 Componenti: Chromosome

Subcellular locationi

  • Cell membrane UniRule annotation; Multi-pass membrane protein UniRule annotation

Topology

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Transmembranei12 – 3221HelicalUniRule annotationAdd
BLAST
Transmembranei37 – 5721HelicalUniRule annotationAdd
BLAST
Transmembranei61 – 8121HelicalUniRule annotationAdd
BLAST

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Cell membrane, Membrane

Pathology & Biotechi

Disruption phenotypei

Essential, it cannot be deleted.1 Publication

Mutagenesis

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Mutagenesisi171 – 1711D → N: No c-di-AMP formation (construct without residues 1-80). 1 Publication
Mutagenesisi172 – 1721G → A: No c-di-AMP formation (construct without residues 1-80). 1 Publication
Mutagenesisi202 – 2021T → N: No c-di-AMP formation (construct without residues 1-80). 1 Publication

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 273273Diadenylate cyclasePRO_0000424179Add
BLAST

Proteomic databases

PaxDbiQ8Y5E4.

Interactioni

Subunit structurei

Homodimer, required for active site formation (Probable).1 Publication

Protein-protein interaction databases

STRINGi169963.lmo2120.

Structurei

Secondary structure

1
273
Legend: HelixTurnBeta strand
Show more details
Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Helixi101 – 12626Combined sources
Beta strandi130 – 1345Combined sources
Beta strandi136 – 1394Combined sources
Helixi141 – 1444Combined sources
Beta strandi147 – 1537Combined sources
Helixi156 – 1627Combined sources
Helixi168 – 1703Combined sources
Beta strandi171 – 1777Combined sources
Beta strandi180 – 1867Combined sources
Helixi202 – 21312Combined sources
Beta strandi217 – 2215Combined sources
Turni223 – 2253Combined sources
Beta strandi228 – 2325Combined sources
Beta strandi235 – 2373Combined sources
Helixi242 – 25312Combined sources

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
4RV7X-ray2.80A/B/C/D101-273[»]
ProteinModelPortaliQ8Y5E4.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini82 – 242161DACPROSITE-ProRule annotationAdd
BLAST

Sequence similaritiesi

Belongs to the adenylate cyclase family. DacA/CdaA subfamily.UniRule annotation
Contains 1 DAC domain.PROSITE-ProRule annotation

Keywords - Domaini

Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiENOG4105C8B. Bacteria.
COG1624. LUCA.
HOGENOMiHOG000054800.
KOiK18672.
OMAiWRDLLDI.
OrthoDBiEOG6DRPM4.
PhylomeDBiQ8Y5E4.

Family and domain databases

HAMAPiMF_01499. DacA.
InterProiIPR014046. c-di-AMP_synthase.
IPR003390. DNA_integrity_scan_DisA_N.
[Graphical view]
PfamiPF02457. DisA_N. 1 hit.
[Graphical view]
PIRSFiPIRSF004793. UCP004793. 1 hit.
TIGRFAMsiTIGR00159. TIGR00159. 1 hit.
PROSITEiPS51794. DAC. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q8Y5E4-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MDFSNMSILH YLANIVDILV VWFVIYKVIM LIRGTKAVQL LKGIFIIIAV
60 70 80 90 100
KLLSGFFGLQ TVEWITDQML TWGFLAIIII FQPELRRALE TLGRGNIFTR
110 120 130 140 150
YGSRIEREQH HLIESIEKST QYMAKRRIGA LISVARDTGM DDYIETGIPL
160 170 180 190 200
NAKISSQLLI NIFIPNTPLH DGAVIIKGNE IASAASYLPL SDSPFLSKEL
210 220 230 240 250
GTRHRAALGI SEVTDSITIV VSEETGGISL TKGGELFRDV SEEELHKILL
260 270
KELVTVTAKK PSIFSKWKGG KSE
Length:273
Mass (Da):30,445
Last modified:March 1, 2002 - v1
Checksum:i878951A75FB06D88
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AL591982 Genomic DNA. Translation: CAD00198.1.
PIRiAH1339.
RefSeqiNP_465644.1. NC_003210.1.
WP_003722380.1. NC_003210.1.

Genome annotation databases

EnsemblBacteriaiCAD00198; CAD00198; CAD00198.
GeneIDi984739.
KEGGilmo:lmo2120.
PATRICi20313450. VBILisMon69206_2172.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AL591982 Genomic DNA. Translation: CAD00198.1.
PIRiAH1339.
RefSeqiNP_465644.1. NC_003210.1.
WP_003722380.1. NC_003210.1.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
4RV7X-ray2.80A/B/C/D101-273[»]
ProteinModelPortaliQ8Y5E4.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi169963.lmo2120.

Proteomic databases

PaxDbiQ8Y5E4.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiCAD00198; CAD00198; CAD00198.
GeneIDi984739.
KEGGilmo:lmo2120.
PATRICi20313450. VBILisMon69206_2172.

Phylogenomic databases

eggNOGiENOG4105C8B. Bacteria.
COG1624. LUCA.
HOGENOMiHOG000054800.
KOiK18672.
OMAiWRDLLDI.
OrthoDBiEOG6DRPM4.
PhylomeDBiQ8Y5E4.

Enzyme and pathway databases

BioCyciLMON169963:LMO2120-MONOMER.
BRENDAi2.7.7.85. 3045.

Family and domain databases

HAMAPiMF_01499. DacA.
InterProiIPR014046. c-di-AMP_synthase.
IPR003390. DNA_integrity_scan_DisA_N.
[Graphical view]
PfamiPF02457. DisA_N. 1 hit.
[Graphical view]
PIRSFiPIRSF004793. UCP004793. 1 hit.
TIGRFAMsiTIGR00159. TIGR00159. 1 hit.
PROSITEiPS51794. DAC. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: ATCC BAA-679 / EGD-e.
  2. "c-di-AMP secreted by intracellular Listeria monocytogenes activates a host type I interferon response."
    Woodward J.J., Iavarone A.T., Portnoy D.A.
    Science 328:1703-1705(2010) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION, GENE NAME, DISRUPTION PHENOTYPE.
  3. "Structural and biochemical analysis of the essential diadenylate cyclase CdaA from Listeria monocytogenes."
    Rosenberg J., Dickmanns A., Neumann P., Gunka K., Arens J., Kaever V., Stuelke J., Ficner R., Commichau F.M.
    J. Biol. Chem. 290:6596-6606(2015) [PubMed] [Europe PMC] [Abstract]
    Cited for: X-RAY CRYSTALLOGRAPHY (2.80 ANGSTROMS) OF 101-273 IN COMPLEX WITH ATP, FUNCTION, COFACTOR, SUBUNIT, MUTAGENESIS OF ASP-171; GLY-172 AND THR-202.
    Strain: ATCC BAA-679 / EGD-e.

Entry informationi

Entry nameiDACA_LISMO
AccessioniPrimary (citable) accession number: Q8Y5E4
Entry historyi
Integrated into UniProtKB/Swiss-Prot: October 16, 2013
Last sequence update: March 1, 2002
Last modified: July 6, 2016
This is version 73 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.