Reviewed, UniProtKB/Swiss-Prot Q8Y551 (FUMC_LISMO)

Names and origin

Protein names

Recommended name:
    Fumarate hydratase class II
      Short name=Fumarase C
    EC=4.2.1.2

Gene names

Name:	fumC
Synonyms:	citG
Ordered Locus Names:	lmo2225

Organism

Listeria monocytogenes [Complete proteome] [HAMAP]

Taxonomic identifier

1639 [NCBI]

Taxonomic lineage

Bacteria › Firmicutes › Bacillales › Listeriaceae › Listeria

Protein attributes

Sequence length	455 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Inferred from homology.

General annotation (Comments)

Catalytic activity	(S)-malate = fumarate + H2O. HAMAP MF_00743
Pathway	Carbohydrate metabolism; tricarboxylic acid cycle. HAMAP MF_00743
Subunit structure	Homotetramer By similarity.
Subcellular location	Cytoplasm By similarity.
Miscellaneous	There are 2 substrate binding sites: the catalytic A site, and the non-catalytic B site that may play a role in the transfer of substrate or product between the active site and the solvent. Alternatively, the B site may bind allosteric effectors By similarity.
Sequence similarities	Belongs to the class-II fumarase/aspartase family. Fumarase subfamily.

Ontologies

Keywords
Biological process	Tricarboxylic acid cycle
Cellular component	Cytoplasm
Molecular function	Lyase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	fumarate metabolic process Inferred from electronic annotation. Source: InterPro tricarboxylic acid cycle Inferred from electronic annotation. Source: HAMAP
Cellular component	tricarboxylic acid cycle enzyme complex Inferred from electronic annotation. Source: InterPro
Molecular function	fumarate hydratase activity Inferred from electronic annotation. Source: HAMAP
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 455	455	Fumarate hydratase class II HAMAP MF_00743		PRO_0000161286
Regions
	Region	122 – 125	4	B site By similarity
	Region	132 – 134	3	Substrate binding By similarity
Sites
	Binding site	98	1	Substrate By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q8Y551-1 [UniParc]. Last modified March 1, 2002. Version 1. Checksum: 5AF66C261A8F844A	FASTA	455	49,626
	10 20 30 40 50 60 MERIERDTLG EISVDATKYW GAQTERSKRN FAIGDNPMPI EIIYAFAQLK KATAKVNAAE 70 80 90 100 110 120 GKLAEEKAIA IGQVCDQIIQ GELDEHFPLV VWQTGSGTQS NMNVNEVIAH VANLTLGEGQ 130 140 150 160 170 180 IHPNDDVNMS QSSNDTFPTA MHIAAYGALV TKLLPEITKM EAVLTEKKNK YMHLVKIGRT 190 200 210 220 230 240 HLQDATPLTL GQEISGWEAC LTNNKNYLET SMKAILPLAI GGTAVGTGLN ASRDFGDKVA 250 260 270 280 290 300 EELMKQTGYP FTSDSNKYFA LTSHSPINFV HGAIRSLASD LMKIANDIRL LASGPRSGIG 310 320 330 340 350 360 ELEIPANEPG SSIMPGKVNP TQCEAITMVA AQVMGNDVTI NVAASQGNFE LNVYKPVIIF 370 380 390 400 410 420 NFLESIKLLA DSMRSFRVHC LEGLTANEKV IETKVNDSLM LVTALNPHIG YEKAAKIAKL 430 440 450 AFDENTTLKE AAIKTGFVTE KEFDLWINPL KMTNL

References

[1]

"Comparative genomics of Listeria species." Glaser P., Frangeul L., Buchrieser C., Rusniok C., Amend A., Baquero F., Berche P., Bloecker H., Brandt P., Chakraborty T., Charbit A., Chetouani F., Couve E., de Daruvar A., Dehoux P., Domann E., Dominguez-Bernal G., Duchaud E. , Durant L., Dussurget O., Entian K.-D., Fsihi H., Garcia-del Portillo F., Garrido P., Gautier L., Goebel W., Gomez-Lopez N., Hain T., Hauf J., Jackson D., Jones L.-M., Kaerst U., Kreft J., Kuhn M., Kunst F., Kurapkat G., Madueno E., Maitournam A., Mata Vicente J., Ng E., Nedjari H., Nordsiek G., Novella S., de Pablos B., Perez-Diaz J.-C., Purcell R., Remmel B., Rose M., Schlueter T., Simoes N., Tierrez A., Vazquez-Boland J.-A., Voss H., Wehland J., Cossart P. Science 294:849-852(2001) [PubMed: 11679669] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC BAA-679 / EGD-e / Serovar 1/2a.

Cross-references

Sequence databases
EMBLGenBankDDBJ	AL591982 Genomic DNA. Translation: CAD00303.1.
PIR	AI1352.
RefSeq	NP_465749.1.
3D structure databases
HSSP	HSSP built from PDB template 1FUR based on UniProtKB P05042.
ModBase	Search...
Genome annotation databases
GeneID	987997.
GenomeReviews	Gene locus lmo2225 in contig AL591824_GR.
KEGG	lmo:lmo2225.
Organism-specific databases
ListiList	LMO02225.
CMR	Search...
Phylogenomic databases
HOGENOM	Q8Y551.
OMA	GSQGHFE.
Enzyme and pathway databases
BioCyc	LMON169963:LMO2225-MON.
BRENDA	4.2.1.2. 96770.
Family and domain databases
HAMAP	MF_00743. [Tree]
InterPro	IPR005677. Fum_hydII. IPR018951. Fumarase_C_C. IPR000362. Fumarate_lyase. IPR020557. Fumarate_lyase_CS. [Graphical view]
Pfam	PF10415. FumaraseC_C. 1 hit. PF00206. Lyase_1. 1 hit. [Graphical view]
PRINTS	PR00149. FUMRATELYASE.
TIGRFAMs	TIGR00979. fumC_II. 1 hit.
PROSITE	PS00163. FUMARATE_LYASES. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

FUMC_LISMO

Accession

Primary (citable) accession number: Q8Y551

Entry history

Integrated into UniProtKB/Swiss-Prot:	December 15, 2003
Last sequence update:	March 1, 2002
Last modified:	November 3, 2009
This is version 46 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)