Reviewed, UniProtKB/Swiss-Prot Q8Y4I3 (TPIS1_LISMO)

Names and origin

Protein names

Recommended name:
    Triosephosphate isomerase 1
      Short name=TIM 1
    EC=5.3.1.1
Alternative name(s):
    Triose-phosphate isomerase 1

Gene names

Name:	tpiA1
Synonyms:	tpi
Ordered Locus Names:	lmo2457

Organism

Listeria monocytogenes [Complete proteome] [HAMAP]

Taxonomic identifier

1639 [NCBI]

Taxonomic lineage

Bacteria › Firmicutes › Bacillales › Listeriaceae › Listeria

Protein attributes

Sequence length	251 AA.
Sequence status	Complete.
Protein existence	Inferred from homology.

General annotation (Comments)

Catalytic activity	D-glyceraldehyde 3-phosphate = glycerone phosphate. HAMAP MF_00147
Pathway	Carbohydrate biosynthesis; gluconeogenesis. HAMAP MF_00147 Carbohydrate degradation; glycolysis; D-glyceraldehyde 3-phosphate from glycerone phosphate: step 1/1. HAMAP MF_00147
Subunit structure	Homodimer By similarity. HAMAP MF_00147
Subcellular location	Cytoplasm Probable HAMAP MF_00147.
Sequence similarities	Belongs to the triosephosphate isomerase family.

Ontologies

Keywords
Biological process	Gluconeogenesis Glycolysis Pentose shunt
Cellular component	Cytoplasm
Molecular function	Isomerase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	gluconeogenesis Inferred from electronic annotation. Source: HAMAP glycolysis Inferred from electronic annotation. Source: HAMAP pentose-phosphate shunt Inferred from electronic annotation. Source: HAMAP
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	triose-phosphate isomerase activity Inferred from electronic annotation. Source: HAMAP
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 251	251	Triosephosphate isomerase 1 HAMAP MF_00147		PRO_0000090242
Sites
	Active site	95	1	Electrophile By similarity
	Active site	167	1	Proton acceptor By similarity
	Binding site	9	1	Substrate By similarity
	Binding site	11	1	Substrate By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q8Y4I3-1 [UniParc]. Last modified March 27, 2002. Version 1. Checksum: 2AEDF1AC5F24CE35	FASTA	251	26,878
	10 20 30 40 50 60 MRKPIIAGNW KMNKTAAKAG QFAEDVKNNV PSSDAVESVV AAPALFLQEL VRLTEGTDLR 70 80 90 100 110 120 VSAQNCYFED EGAFTGEISP FALADLGVSY VIIGHSERRE YFHETDEDIN KKAHAIFKHG 130 140 150 160 170 180 MTPIICCGET LDQREAGQTD TWVRGQIRAA LAGLTEEQVI KSVIAYEPIW AIGTGKSSTS 190 200 210 220 230 240 ADANETCAVI RAEVADAVSQ KAADAVRIQY GGSVKPENIA DYLAESDIDG ALVGGASLEP 250 ASFLALLEAV K

References

[1]

"Comparative genomics of Listeria species." Glaser P., Frangeul L., Buchrieser C., Rusniok C., Amend A., Baquero F., Berche P., Bloecker H., Brandt P., Chakraborty T., Charbit A., Chetouani F., Couve E., de Daruvar A., Dehoux P., Domann E., Dominguez-Bernal G., Duchaud E. , Durant L., Dussurget O., Entian K.-D., Fsihi H., Garcia-del Portillo F., Garrido P., Gautier L., Goebel W., Gomez-Lopez N., Hain T., Hauf J., Jackson D., Jones L.-M., Kaerst U., Kreft J., Kuhn M., Kunst F., Kurapkat G., Madueno E., Maitournam A., Mata Vicente J., Ng E., Nedjari H., Nordsiek G., Novella S., de Pablos B., Perez-Diaz J.-C., Purcell R., Remmel B., Rose M., Schlueter T., Simoes N., Tierrez A., Vazquez-Boland J.-A., Voss H., Wehland J., Cossart P. Science 294:849-852(2001) [PubMed: 11679669] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC BAA-679 / EGD-e / Serovar 1/2a.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AL591983 Genomic DNA. Translation: CAD00535.1.
PIR	AI1381.
RefSeq	NP_465980.1.
3D structure databases
SMR	Q8Y4I3. Positions 2-249.
ModBase	Search...
Genome annotation databases
GeneID	987380.
KEGG	lmo:lmo2457.
Organism-specific databases
ListiList	LMO02457.
CMR	Search...
Phylogenomic databases
HOGENOM	HBG708281.
OMA	DIRSVQT.
Enzyme and pathway databases
BioCyc	LMON169963:LMO2457-MONOMER.
BRENDA	5.3.1.1. 96770.
Family and domain databases
HAMAP	MF_00147_B. TIM_B. [Tree]
InterPro	IPR013785. Aldolase_TIM. IPR000652. Triosephosphate_isomerase. IPR020861. Triosephosphate_isomerase_AS. [Graphical view]
Gene3D	G3DSA:3.20.20.70. Aldolase_TIM. 1 hit.
PANTHER	PTHR21139. Triophos_ismrse. 1 hit.
Pfam	PF00121. TIM. 1 hit. [Graphical view]
TIGRFAMs	TIGR00419. tim. 1 hit.
PROSITE	PS00171. TIM_1. 1 hit. PS51440. TIM_2. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

TPIS1_LISMO

Accession

Primary (citable) accession number: Q8Y4I3

Entry history

Integrated into UniProtKB/Swiss-Prot:	March 27, 2002
Last sequence update:	March 27, 2002
Last modified:	February 9, 2010
This is version 52 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)