Reviewed, UniProtKB/Swiss-Prot Q8Y4H1 (NAMA_LISMO)

Names and origin

Protein names

Recommended name:
    NADPH dehydrogenase
    EC=1.6.99.1
Alternative name(s):
    Xenobiotic reductase

Gene names

Name:	namA
Ordered Locus Names:	lmo2471

Organism

Listeria monocytogenes [Complete proteome] [HAMAP]

Taxonomic identifier

1639 [NCBI]

Taxonomic lineage

Bacteria › Firmicutes › Bacillales › Listeriaceae › Listeria

Protein attributes

Sequence length	338 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Inferred from homology.

General annotation (Comments)

Function	Catalyzes the reduction of the double bond of an array of alpha, beta-unsaturated aldehydes and ketones. It also reduces the nitro group of nitroester and nitroaromatic compounds. It could have a role in detoxification processes By similarity.
Catalytic activity	NADPH + acceptor = NADP(+) + reduced acceptor.
Cofactor	FMN By similarity.
Subunit structure	Homotetramer By similarity.
Sequence similarities	Belongs to the NADH:flavin oxidoreductase/NADH oxidase family. NamA subfamily.

Ontologies

Keywords
Ligand	FMN Flavoprotein NADP
Molecular function	Oxidoreductase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	oxidation reduction Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	FMN binding Inferred from electronic annotation. Source: InterPro NADPH dehydrogenase activity Inferred from electronic annotation. Source: HAMAP
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 338	338	NADPH dehydrogenase		PRO_0000216124
Regions
	Nucleotide binding	22 – 26	5	FMN By similarity
Sites
	Binding site	27	1	Substrate By similarity
	Binding site	163	1	Substrate By similarity
	Binding site	166	1	Substrate By similarity
	Binding site	214	1	FMN By similarity
	Binding site	307	1	FMN By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q8Y4H1-1 [UniParc]. Last modified March 1, 2002. Version 1. Checksum: FEBBFF68FEB305A0	FASTA	338	37,005
	10 20 30 40 50 60 MSKLFSEYKL KDVTLKNRIV MSPMCMYSVE NKDGIATDFH FAHYVSRAAG GTGLVILEAT 70 80 90 100 110 120 AVQEVGRISE FDLGLWNDEQ VPALKKLVGG LHYHGAKAGI QLAHAGRKAV LPGEIVAPSA 130 140 150 160 170 180 IAFDEKSDKP VELTKEAIKE VVADFKRAAY RAKEAGFDVI EIHAAHGYLI HQFLSPITNR 190 200 210 220 230 240 REDNYGGPAG NRYKILSDII KAVKEVWDGP IIVRVSATDY AHGGLQLEDH IPFAKWMKAD 250 260 270 280 290 300 GVELIDVSTG GLVNVAPPVF PGYQVPFADE IRRGAGIATG ALGLITRGEQ AEEILCNERA 310 320 330 DLIIVGRELL RNPYFAKDAA KQLGETIEAP KQYSRAWK

References

[1]

"Comparative genomics of Listeria species." Glaser P., Frangeul L., Buchrieser C., Rusniok C., Amend A., Baquero F., Berche P., Bloecker H., Brandt P., Chakraborty T., Charbit A., Chetouani F., Couve E., de Daruvar A., Dehoux P., Domann E., Dominguez-Bernal G., Duchaud E. , Durant L., Dussurget O., Entian K.-D., Fsihi H., Garcia-del Portillo F., Garrido P., Gautier L., Goebel W., Gomez-Lopez N., Hain T., Hauf J., Jackson D., Jones L.-M., Kaerst U., Kreft J., Kuhn M., Kunst F., Kurapkat G., Madueno E., Maitournam A., Mata Vicente J., Ng E., Nedjari H., Nordsiek G., Novella S., de Pablos B., Perez-Diaz J.-C., Purcell R., Remmel B., Rose M., Schlueter T., Simoes N., Tierrez A., Vazquez-Boland J.-A., Voss H., Wehland J., Cossart P. Science 294:849-852(2001) [PubMed: 11679669] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC BAA-679 / EGD-e / Serovar 1/2a.

Cross-references

Sequence databases
EMBLGenBankDDBJ	AL591983 Genomic DNA. Translation: CAD00549.1.
PIR	AG1383.
RefSeq	NP_465994.1.
3D structure databases
HSSP	HSSP built from PDB template 1GVR based on UniProtKB P71278.
ModBase	Search...
Genome annotation databases
GeneID	987354.
GenomeReviews	Gene locus lmo2471 in contig AL591824_GR.
KEGG	lmo:lmo2471.
Organism-specific databases
ListiList	LMO2471.
CMR	Search...
Phylogenomic databases
HOGENOM	Q8Y4H1.
Enzyme and pathway databases
BioCyc	LMON169963:LMO2471-MON.
Family and domain databases
HAMAP	MF_01614. [Tree]
InterPro	IPR013785. Aldolase_TIM. IPR001155. OxRdtase_FMN_N. [Graphical view]
Gene3D	G3DSA:3.20.20.70. Aldolase_TIM. 1 hit.
Pfam	PF00724. Oxidored_FMN. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

NAMA_LISMO

Accession

Primary (citable) accession number: Q8Y4H1

Entry history

Integrated into UniProtKB/Swiss-Prot:	June 21, 2005
Last sequence update:	March 1, 2002
Last modified:	November 25, 2008
This is version 31 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)