Reviewed, UniProtKB/Swiss-Prot Q8Y4C4 (MURA1_LISMO)

Names and origin

Protein names

Recommended name:
    UDP-N-acetylglucosamine 1-carboxyvinyltransferase 1
    EC=2.5.1.7
Alternative name(s):
    Enoylpyruvate transferase 1
    UDP-N-acetylglucosamine enolpyruvyl transferase 1
      Short name=EPT 1

Gene names

Name:	murA1
Synonyms:	murA
Ordered Locus Names:	lmo2526

Organism

Listeria monocytogenes [Complete proteome] [HAMAP]

Taxonomic identifier

1639 [NCBI]

Taxonomic lineage

Bacteria › Firmicutes › Bacillales › Listeriaceae › Listeria

Protein attributes

Sequence length	430 AA.
Sequence status	Complete.
Protein existence	Inferred from homology.

General annotation (Comments)

Function	Cell wall formation. Adds enolpyruvyl to UDP-N-acetylglucosamine By similarity. HAMAP MF_00111
Catalytic activity	Phosphoenolpyruvate + UDP-N-acetyl-D-glucosamine = phosphate + UDP-N-acetyl-3-O-(1-carboxyvinyl)-D-glucosamine. HAMAP MF_00111
Pathway	Cell wall biogenesis; peptidoglycan biosynthesis. HAMAP MF_00111
Subcellular location	Cytoplasm Probable HAMAP MF_00111.
Sequence similarities	Belongs to the EPSP synthase family. MurA subfamily.

Ontologies

Keywords
Biological process	Cell cycle Cell division Cell shape Cell wall biogenesis/degradation Peptidoglycan synthesis
Cellular component	Cytoplasm
Molecular function	Transferase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	UDP-N-acetylgalactosamine biosynthetic process Inferred from electronic annotation. Source: InterPro cell cycle Inferred from electronic annotation. Source: UniProtKB-KW cell division Inferred from electronic annotation. Source: UniProtKB-KW cellular cell wall organization Inferred from electronic annotation. Source: UniProtKB-KW peptidoglycan biosynthetic process Inferred from electronic annotation. Source: HAMAP regulation of cell shape Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	UDP-N-acetylglucosamine 1-carboxyvinyltransferase activity Inferred from electronic annotation. Source: HAMAP
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 430	430	UDP-N-acetylglucosamine 1-carboxyvinyltransferase 1 HAMAP MF_00111		PRO_0000178887
Sites
	Active site	117	1	Proton donor By similarity
	Binding site	117	1	Phosphoenolpyruvate (covalent) By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q8Y4C4-1 [UniParc]. Last modified March 1, 2002. Version 1. Checksum: F73905754D13BFB9	FASTA	430	45,979
	10 20 30 40 50 60 MEKIIVRGGK QLNGSVKMEG AKNAVLPVIA ATLLASKGTS VLKNVPNLSD VFTINEVLKY 70 80 90 100 110 120 LNADVSFVND EVTVDATGEI TSDAPFEYVR KMRASIVVMG PLLARTGSAR VALPGGCAIG 130 140 150 160 170 180 SRPVDLHLKG FEAMGAVVKI ENGYIEATAE KLVGAKVYLD FPSVGATQNI MMAATLAEGT 190 200 210 220 230 240 TVIENVAREP EIVDLANFLN QMGARVIGAG TEVIRIEGVK ELTATEHSII PDRIEAGTFM 250 260 270 280 290 300 IAAAITGGNV LIEDAVPEHI SSLIAKLEEM GVQIIEEENG IRVIGPDKLK AVDVKTMPHP 310 320 330 340 350 360 GFPTDMQSQM MVIQMLSEGT SIMTETVFEN RFMHVEEMRR MNADMKIEGH SVIISGPAKL 370 380 390 400 410 420 QGAEVAATDL RAAAALILAG LVADGYTQVT ELKYLDRGYN NFHGKLQALG ADVERVDDSK 430 VDVTNLASLF

References

[1]

"Comparative genomics of Listeria species." Glaser P., Frangeul L., Buchrieser C., Rusniok C., Amend A., Baquero F., Berche P., Bloecker H., Brandt P., Chakraborty T., Charbit A., Chetouani F., Couve E., de Daruvar A., Dehoux P., Domann E., Dominguez-Bernal G., Duchaud E. , Durant L., Dussurget O., Entian K.-D., Fsihi H., Garcia-del Portillo F., Garrido P., Gautier L., Goebel W., Gomez-Lopez N., Hain T., Hauf J., Jackson D., Jones L.-M., Kaerst U., Kreft J., Kuhn M., Kunst F., Kurapkat G., Madueno E., Maitournam A., Mata Vicente J., Ng E., Nedjari H., Nordsiek G., Novella S., de Pablos B., Perez-Diaz J.-C., Purcell R., Remmel B., Rose M., Schlueter T., Simoes N., Tierrez A., Vazquez-Boland J.-A., Voss H., Wehland J., Cossart P. Science 294:849-852(2001) [PubMed: 11679669] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC BAA-679 / EGD-e / Serovar 1/2a.

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Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AL591983 Genomic DNA. Translation: CAD00604.1.
PIR	AF1390.
RefSeq	NP_466049.1.
3D structure databases
SMR	Q8Y4C4. Positions 1-417.
ModBase	Search...
Genome annotation databases
GeneID	984514.
KEGG	lmo:lmo2526.
Organism-specific databases
ListiList	LMO02526.
CMR	Search...
Phylogenomic databases
HOGENOM	HBG482701.
OMA	VSRVYHL.
Enzyme and pathway databases
BioCyc	LMON169963:LMO2526-MONOMER.
BRENDA	2.5.1.7. 96770.
Family and domain databases
HAMAP	MF_00111. MurA. [Tree]
InterPro	IPR001986. EPSP_synthase_core. IPR013792. RNA3'P_cycl/enolpyr_Trfase_a/b. IPR005750. UDP_GlcNAc_COvinyl_MurA. [Graphical view]
Gene3D	G3DSA:3.65.10.10. EPSP_synthase. 2 hits.
PANTHER	PTHR21090:SF4. AcGlu_Tran_MurA. 1 hit.
Pfam	PF00275. EPSP_synthase. 1 hit. [Graphical view]
TIGRFAMs	TIGR01072. murA. 1 hit.
ProtoNet	Search...

Entry information

Entry name

MURA1_LISMO

Accession

Primary (citable) accession number: Q8Y4C4

Entry history

Integrated into UniProtKB/Swiss-Prot:	August 30, 2002
Last sequence update:	March 1, 2002
Last modified:	February 9, 2010
This is version 56 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)