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Protein

Phosphoenolpyruvate carboxykinase [GTP]

Gene

pckG

Organism
Ralstonia solanacearum (strain GMI1000) (Pseudomonas solanacearum)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Protein inferred from homologyi

Functioni

Catalyzes the conversion of oxaloacetate (OAA) to phosphoenolpyruvate (PEP), the rate-limiting step in the metabolic pathway that produces glucose from lactate and other precursors derived from the citric acid cycle.UniRule annotation

Catalytic activityi

GTP + oxaloacetate = GDP + phosphoenolpyruvate + CO2.UniRule annotation

Cofactori

Mn2+UniRule annotationNote: Binds 1 Mn2+ ion per subunit.UniRule annotation

Pathway:igluconeogenesis

This protein is involved in the pathway gluconeogenesis, which is part of Carbohydrate biosynthesis.UniRule annotation
View all proteins of this organism that are known to be involved in the pathway gluconeogenesis and in Carbohydrate biosynthesis.

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei85 – 851SubstrateUniRule annotation
Metal bindingi233 – 2331ManganeseUniRule annotation
Metal bindingi253 – 2531Manganese; via tele nitrogenUniRule annotation
Binding sitei275 – 2751SubstrateUniRule annotation
Active sitei277 – 2771UniRule annotation
Metal bindingi302 – 3021ManganeseUniRule annotation
Binding sitei402 – 4021GTPUniRule annotation
Binding sitei433 – 4331GTPUniRule annotation

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Nucleotide bindingi276 – 2816GTPUniRule annotation
Nucleotide bindingi530 – 5334GTPUniRule annotation

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Decarboxylase, Lyase

Keywords - Biological processi

Gluconeogenesis

Keywords - Ligandi

GTP-binding, Manganese, Metal-binding, Nucleotide-binding

Enzyme and pathway databases

BioCyciRSOL267608:GCVU-17-MONOMER.
UniPathwayiUPA00138.

Names & Taxonomyi

Protein namesi
Recommended name:
Phosphoenolpyruvate carboxykinase [GTP]UniRule annotation (EC:4.1.1.32UniRule annotation)
Short name:
PEP carboxykinaseUniRule annotation
Short name:
PEPCKUniRule annotation
Gene namesi
Name:pckGUniRule annotation
Synonyms:pckA
Ordered Locus Names:RSc0017
ORF Names:RS01839
OrganismiRalstonia solanacearum (strain GMI1000) (Pseudomonas solanacearum)
Taxonomic identifieri267608 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaBetaproteobacteriaBurkholderialesBurkholderiaceaeRalstonia
ProteomesiUP000001436 Componenti: Chromosome

Subcellular locationi

  • Cytoplasm UniRule annotation

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 622622Phosphoenolpyruvate carboxykinase [GTP]PRO_0000103613Add
BLAST

Interactioni

Subunit structurei

Monomer.UniRule annotation

Protein-protein interaction databases

STRINGi267608.RSc0017.

Structurei

3D structure databases

ProteinModelPortaliQ8Y3G3.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni224 – 2263Substrate bindingUniRule annotation
Regioni400 – 4023Substrate bindingUniRule annotation

Sequence similaritiesi

Belongs to the phosphoenolpyruvate carboxykinase [GTP] family.UniRule annotation

Phylogenomic databases

eggNOGiCOG1274.
HOGENOMiHOG000191700.
KOiK01596.
OMAiWMRFGED.
OrthoDBiEOG6MPWQS.

Family and domain databases

Gene3Di3.40.449.10. 1 hit.
3.90.228.20. 2 hits.
HAMAPiMF_00452. PEPCK_GTP.
InterProiIPR018091. PEP_carboxykin_GTP_CS.
IPR013035. PEP_carboxykinase_C.
IPR008209. PEP_carboxykinase_GTP.
IPR008210. PEP_carboxykinase_N.
[Graphical view]
PANTHERiPTHR11561. PTHR11561. 1 hit.
PfamiPF00821. PEPCK. 1 hit.
[Graphical view]
PIRSFiPIRSF001348. PEP_carboxykinase_GTP. 1 hit.
SUPFAMiSSF68923. SSF68923. 1 hit.
PROSITEiPS00505. PEPCK_GTP. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q8Y3G3-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MNQPVMQGVP ALNVPAYVKH ARLVAWVSEI AALTKPERVV WCDGSQEEYD
60 70 80 90 100
RLCAEMVAAG TLKQLNPAKR KNSYLALSDP SDVARVEDRT FICSQKQEDA
110 120 130 140 150
GPTNNWTAPA EMRQTLNGLF DGCMRGRTLY VVPFSMGPLG SPIAHIGVEL
160 170 180 190 200
SDSPYVAVNM RIMTRMGRAV YDVLGADGEF VPCVHTVGKP LAAGEQDVPW
210 220 230 240 250
PCNPTKYIVH FPETREIWSF GSGYGGNALL GKKCFALRIA STMGRDQGWL
260 270 280 290 300
AEHMLILGVT SPEGKTYHVA AAFPSACGKT NFAMLIPPAG FDGWKVTTIG
310 320 330 340 350
DDIAWIKPRQ DANGQTRLYA INPEAGFFGV APGTGEKTNF NAMATLKENV
360 370 380 390 400
IFTNVALTDD GDVWWEGMTD TPPAHLTDWQ GQDWTPAIAK ETGRKAAHPN
410 420 430 440 450
SRFTAPAAQC PSIDPEWDNP AGVAIDAFIF GGRRSTTVPL VTEARDWTEG
460 470 480 490 500
VYMAATMGSE TTAAAVGQQG VVRRDPFAML PFCGYNMADY FAHWLKLGDQ
510 520 530 540 550
LAKSGAELPK IFCVNWFRKD EQGRFVWPGF GENMRVLKWM IDRIEGQARG
560 570 580 590 600
DEHVFGVSPR YEELRWDGLD FSAEQFAKVI SLDAQAWQQE LTLHAELFAQ
610 620
LAHHLPQALP EAKARLEARL QG
Length:622
Mass (Da):68,462
Last modified:March 1, 2002 - v1
Checksum:i6E6E16F6E90A61FE
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AL646052 Genomic DNA. Translation: CAD13545.1.
RefSeqiWP_010999984.1. NC_003295.1.

Genome annotation databases

EnsemblBacteriaiCAD13545; CAD13545; RSc0017.
GeneIDi1218819.
KEGGirso:RSc0017.
PATRICi20258565. VBIRalSol70888_0018.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AL646052 Genomic DNA. Translation: CAD13545.1.
RefSeqiWP_010999984.1. NC_003295.1.

3D structure databases

ProteinModelPortaliQ8Y3G3.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi267608.RSc0017.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiCAD13545; CAD13545; RSc0017.
GeneIDi1218819.
KEGGirso:RSc0017.
PATRICi20258565. VBIRalSol70888_0018.

Phylogenomic databases

eggNOGiCOG1274.
HOGENOMiHOG000191700.
KOiK01596.
OMAiWMRFGED.
OrthoDBiEOG6MPWQS.

Enzyme and pathway databases

UniPathwayiUPA00138.
BioCyciRSOL267608:GCVU-17-MONOMER.

Family and domain databases

Gene3Di3.40.449.10. 1 hit.
3.90.228.20. 2 hits.
HAMAPiMF_00452. PEPCK_GTP.
InterProiIPR018091. PEP_carboxykin_GTP_CS.
IPR013035. PEP_carboxykinase_C.
IPR008209. PEP_carboxykinase_GTP.
IPR008210. PEP_carboxykinase_N.
[Graphical view]
PANTHERiPTHR11561. PTHR11561. 1 hit.
PfamiPF00821. PEPCK. 1 hit.
[Graphical view]
PIRSFiPIRSF001348. PEP_carboxykinase_GTP. 1 hit.
SUPFAMiSSF68923. SSF68923. 1 hit.
PROSITEiPS00505. PEPCK_GTP. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

  1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: GMI1000.

Entry informationi

Entry nameiPCKG_RALSO
AccessioniPrimary (citable) accession number: Q8Y3G3
Entry historyi
Integrated into UniProtKB/Swiss-Prot: May 27, 2002
Last sequence update: March 1, 2002
Last modified: July 22, 2015
This is version 84 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.