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Q8Y3G3

- PCKG_RALSO

UniProt

Q8Y3G3 - PCKG_RALSO

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Protein
Phosphoenolpyruvate carboxykinase [GTP]
Gene
pckG, pckA, RSc0017, RS01839
Organism
Ralstonia solanacearum (strain GMI1000) (Pseudomonas solanacearum)
Status
Reviewed - Annotation score: 3 out of 5 - Protein inferred from homologyi

Functioni

Catalyzes the conversion of oxaloacetate (OAA) to phosphoenolpyruvate (PEP), the rate-limiting step in the metabolic pathway that produces glucose from lactate and other precursors derived from the citric acid cycle By similarity.UniRule annotation

Catalytic activityi

GTP + oxaloacetate = GDP + phosphoenolpyruvate + CO2.UniRule annotation

Cofactori

Binds 1 manganese ion per subunit By similarity.UniRule annotation

Pathwayi

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei85 – 851Substrate By similarity
Binding sitei226 – 2261Substrate; via amide nitrogen By similarity
Metal bindingi233 – 2331Manganese By similarity
Binding sitei233 – 2331Substrate By similarity
Metal bindingi253 – 2531Manganese; via tele nitrogen By similarity
Binding sitei275 – 2751Substrate By similarity
Active sitei277 – 2771 By similarity
Metal bindingi302 – 3021Manganese By similarity
Binding sitei402 – 4021GTP By similarity
Binding sitei433 – 4331GTP By similarity

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Nucleotide bindingi276 – 2816GTP By similarity
Nucleotide bindingi530 – 5334GTP By similarity

GO - Molecular functioni

  1. GTP binding Source: UniProtKB-HAMAP
  2. manganese ion binding Source: UniProtKB-HAMAP
  3. phosphoenolpyruvate carboxykinase (GTP) activity Source: UniProtKB-HAMAP

GO - Biological processi

  1. gluconeogenesis Source: UniProtKB-HAMAP
Complete GO annotation...

Keywords - Molecular functioni

Decarboxylase, Lyase

Keywords - Biological processi

Gluconeogenesis

Keywords - Ligandi

GTP-binding, Manganese, Metal-binding, Nucleotide-binding

Enzyme and pathway databases

BioCyciRSOL267608:GCVU-17-MONOMER.
UniPathwayiUPA00138.

Names & Taxonomyi

Protein namesi
Recommended name:
Phosphoenolpyruvate carboxykinase [GTP] (EC:4.1.1.32)
Short name:
PEP carboxykinase
Short name:
PEPCK
Gene namesi
Name:pckG
Synonyms:pckA
Ordered Locus Names:RSc0017
ORF Names:RS01839
OrganismiRalstonia solanacearum (strain GMI1000) (Pseudomonas solanacearum)
Taxonomic identifieri267608 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaBetaproteobacteriaBurkholderialesBurkholderiaceaeRalstonia
ProteomesiUP000001436: Chromosome

Subcellular locationi

Cytoplasm By similarity UniRule annotation

GO - Cellular componenti

  1. cytoplasm Source: UniProtKB-SubCell
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 622622Phosphoenolpyruvate carboxykinase [GTP]UniRule annotation
PRO_0000103613Add
BLAST

Interactioni

Subunit structurei

Monomer By similarity.UniRule annotation

Protein-protein interaction databases

STRINGi267608.RSc0017.

Structurei

3D structure databases

ProteinModelPortaliQ8Y3G3.

Family & Domainsi

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni400 – 4023Substrate binding By similarity

Sequence similaritiesi

Phylogenomic databases

eggNOGiCOG1274.
HOGENOMiHOG000191700.
KOiK01596.
OMAiWDNPAGV.
OrthoDBiEOG6MPWQS.

Family and domain databases

Gene3Di3.40.449.10. 1 hit.
3.90.228.20. 2 hits.
HAMAPiMF_00452. PEPCK_GTP.
InterProiIPR018091. PEP_carboxykin_GTP_CS.
IPR013035. PEP_carboxykinase_C.
IPR008209. PEP_carboxykinase_GTP.
IPR008210. PEP_carboxykinase_N.
[Graphical view]
PANTHERiPTHR11561. PTHR11561. 1 hit.
PfamiPF00821. PEPCK. 1 hit.
[Graphical view]
PIRSFiPIRSF001348. PEP_carboxykinase_GTP. 1 hit.
SUPFAMiSSF68923. SSF68923. 1 hit.
PROSITEiPS00505. PEPCK_GTP. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q8Y3G3-1 [UniParc]FASTAAdd to Basket

« Hide

MNQPVMQGVP ALNVPAYVKH ARLVAWVSEI AALTKPERVV WCDGSQEEYD    50
RLCAEMVAAG TLKQLNPAKR KNSYLALSDP SDVARVEDRT FICSQKQEDA 100
GPTNNWTAPA EMRQTLNGLF DGCMRGRTLY VVPFSMGPLG SPIAHIGVEL 150
SDSPYVAVNM RIMTRMGRAV YDVLGADGEF VPCVHTVGKP LAAGEQDVPW 200
PCNPTKYIVH FPETREIWSF GSGYGGNALL GKKCFALRIA STMGRDQGWL 250
AEHMLILGVT SPEGKTYHVA AAFPSACGKT NFAMLIPPAG FDGWKVTTIG 300
DDIAWIKPRQ DANGQTRLYA INPEAGFFGV APGTGEKTNF NAMATLKENV 350
IFTNVALTDD GDVWWEGMTD TPPAHLTDWQ GQDWTPAIAK ETGRKAAHPN 400
SRFTAPAAQC PSIDPEWDNP AGVAIDAFIF GGRRSTTVPL VTEARDWTEG 450
VYMAATMGSE TTAAAVGQQG VVRRDPFAML PFCGYNMADY FAHWLKLGDQ 500
LAKSGAELPK IFCVNWFRKD EQGRFVWPGF GENMRVLKWM IDRIEGQARG 550
DEHVFGVSPR YEELRWDGLD FSAEQFAKVI SLDAQAWQQE LTLHAELFAQ 600
LAHHLPQALP EAKARLEARL QG 622
Length:622
Mass (Da):68,462
Last modified:March 1, 2002 - v1
Checksum:i6E6E16F6E90A61FE
GO

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AL646052 Genomic DNA. Translation: CAD13545.1.
RefSeqiNP_518138.1. NC_003295.1.
WP_010999984.1. NC_003295.1.

Genome annotation databases

EnsemblBacteriaiCAD13545; CAD13545; RSc0017.
GeneIDi1218819.
KEGGirso:RSc0017.
PATRICi20258565. VBIRalSol70888_0018.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AL646052 Genomic DNA. Translation: CAD13545.1 .
RefSeqi NP_518138.1. NC_003295.1.
WP_010999984.1. NC_003295.1.

3D structure databases

ProteinModelPortali Q8Y3G3.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

STRINGi 267608.RSc0017.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblBacteriai CAD13545 ; CAD13545 ; RSc0017 .
GeneIDi 1218819.
KEGGi rso:RSc0017.
PATRICi 20258565. VBIRalSol70888_0018.

Phylogenomic databases

eggNOGi COG1274.
HOGENOMi HOG000191700.
KOi K01596.
OMAi WDNPAGV.
OrthoDBi EOG6MPWQS.

Enzyme and pathway databases

UniPathwayi UPA00138 .
BioCyci RSOL267608:GCVU-17-MONOMER.

Family and domain databases

Gene3Di 3.40.449.10. 1 hit.
3.90.228.20. 2 hits.
HAMAPi MF_00452. PEPCK_GTP.
InterProi IPR018091. PEP_carboxykin_GTP_CS.
IPR013035. PEP_carboxykinase_C.
IPR008209. PEP_carboxykinase_GTP.
IPR008210. PEP_carboxykinase_N.
[Graphical view ]
PANTHERi PTHR11561. PTHR11561. 1 hit.
Pfami PF00821. PEPCK. 1 hit.
[Graphical view ]
PIRSFi PIRSF001348. PEP_carboxykinase_GTP. 1 hit.
SUPFAMi SSF68923. SSF68923. 1 hit.
PROSITEi PS00505. PEPCK_GTP. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

  1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: GMI1000.

Entry informationi

Entry nameiPCKG_RALSO
AccessioniPrimary (citable) accession number: Q8Y3G3
Entry historyi
Integrated into UniProtKB/Swiss-Prot: May 27, 2002
Last sequence update: March 1, 2002
Last modified: September 3, 2014
This is version 77 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

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