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Q8Y3G3

- PCKG_RALSO

UniProt

Q8Y3G3 - PCKG_RALSO

Protein

Phosphoenolpyruvate carboxykinase [GTP]

Gene

pckG

Organism
Ralstonia solanacearum (strain GMI1000) (Pseudomonas solanacearum)
Status
Reviewed - Annotation score: 3 out of 5- Protein inferred from homologyi
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    • History
      Entry version 78 (01 Oct 2014)
      Sequence version 1 (01 Mar 2002)
      Previous versions | rss
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    Functioni

    Catalyzes the conversion of oxaloacetate (OAA) to phosphoenolpyruvate (PEP), the rate-limiting step in the metabolic pathway that produces glucose from lactate and other precursors derived from the citric acid cycle.UniRule annotation

    Catalytic activityi

    GTP + oxaloacetate = GDP + phosphoenolpyruvate + CO2.UniRule annotation

    Cofactori

    Binds 1 manganese ion per subunit.UniRule annotation

    Pathwayi

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Binding sitei85 – 851SubstrateUniRule annotation
    Binding sitei226 – 2261Substrate; via amide nitrogenUniRule annotation
    Metal bindingi233 – 2331ManganeseUniRule annotation
    Binding sitei233 – 2331SubstrateUniRule annotation
    Metal bindingi253 – 2531Manganese; via tele nitrogenUniRule annotation
    Binding sitei275 – 2751SubstrateUniRule annotation
    Active sitei277 – 2771UniRule annotation
    Metal bindingi302 – 3021ManganeseUniRule annotation
    Binding sitei402 – 4021GTPUniRule annotation
    Binding sitei433 – 4331GTPUniRule annotation

    Regions

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Nucleotide bindingi276 – 2816GTPUniRule annotation
    Nucleotide bindingi530 – 5334GTPUniRule annotation

    GO - Molecular functioni

    1. GTP binding Source: UniProtKB-HAMAP
    2. manganese ion binding Source: UniProtKB-HAMAP
    3. phosphoenolpyruvate carboxykinase (GTP) activity Source: UniProtKB-HAMAP

    GO - Biological processi

    1. gluconeogenesis Source: UniProtKB-HAMAP

    Keywords - Molecular functioni

    Decarboxylase, Lyase

    Keywords - Biological processi

    Gluconeogenesis

    Keywords - Ligandi

    GTP-binding, Manganese, Metal-binding, Nucleotide-binding

    Enzyme and pathway databases

    BioCyciRSOL267608:GCVU-17-MONOMER.
    UniPathwayiUPA00138.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Phosphoenolpyruvate carboxykinase [GTP]UniRule annotation (EC:4.1.1.32UniRule annotation)
    Short name:
    PEP carboxykinaseUniRule annotation
    Short name:
    PEPCKUniRule annotation
    Gene namesi
    Name:pckGUniRule annotation
    Synonyms:pckA
    Ordered Locus Names:RSc0017
    ORF Names:RS01839
    OrganismiRalstonia solanacearum (strain GMI1000) (Pseudomonas solanacearum)
    Taxonomic identifieri267608 [NCBI]
    Taxonomic lineageiBacteriaProteobacteriaBetaproteobacteriaBurkholderialesBurkholderiaceaeRalstonia
    ProteomesiUP000001436: Chromosome

    Subcellular locationi

    Cytoplasm UniRule annotation

    GO - Cellular componenti

    1. cytoplasm Source: UniProtKB-SubCell

    Keywords - Cellular componenti

    Cytoplasm

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 622622Phosphoenolpyruvate carboxykinase [GTP]PRO_0000103613Add
    BLAST

    Interactioni

    Subunit structurei

    Monomer.UniRule annotation

    Protein-protein interaction databases

    STRINGi267608.RSc0017.

    Structurei

    3D structure databases

    ProteinModelPortaliQ8Y3G3.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Region

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Regioni400 – 4023Substrate bindingUniRule annotation

    Sequence similaritiesi

    Belongs to the phosphoenolpyruvate carboxykinase [GTP] family.UniRule annotation

    Phylogenomic databases

    eggNOGiCOG1274.
    HOGENOMiHOG000191700.
    KOiK01596.
    OMAiWDNPAGV.
    OrthoDBiEOG6MPWQS.

    Family and domain databases

    Gene3Di3.40.449.10. 1 hit.
    3.90.228.20. 2 hits.
    HAMAPiMF_00452. PEPCK_GTP.
    InterProiIPR018091. PEP_carboxykin_GTP_CS.
    IPR013035. PEP_carboxykinase_C.
    IPR008209. PEP_carboxykinase_GTP.
    IPR008210. PEP_carboxykinase_N.
    [Graphical view]
    PANTHERiPTHR11561. PTHR11561. 1 hit.
    PfamiPF00821. PEPCK. 1 hit.
    [Graphical view]
    PIRSFiPIRSF001348. PEP_carboxykinase_GTP. 1 hit.
    SUPFAMiSSF68923. SSF68923. 1 hit.
    PROSITEiPS00505. PEPCK_GTP. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    Q8Y3G3-1 [UniParc]FASTAAdd to Basket

    « Hide

    MNQPVMQGVP ALNVPAYVKH ARLVAWVSEI AALTKPERVV WCDGSQEEYD    50
    RLCAEMVAAG TLKQLNPAKR KNSYLALSDP SDVARVEDRT FICSQKQEDA 100
    GPTNNWTAPA EMRQTLNGLF DGCMRGRTLY VVPFSMGPLG SPIAHIGVEL 150
    SDSPYVAVNM RIMTRMGRAV YDVLGADGEF VPCVHTVGKP LAAGEQDVPW 200
    PCNPTKYIVH FPETREIWSF GSGYGGNALL GKKCFALRIA STMGRDQGWL 250
    AEHMLILGVT SPEGKTYHVA AAFPSACGKT NFAMLIPPAG FDGWKVTTIG 300
    DDIAWIKPRQ DANGQTRLYA INPEAGFFGV APGTGEKTNF NAMATLKENV 350
    IFTNVALTDD GDVWWEGMTD TPPAHLTDWQ GQDWTPAIAK ETGRKAAHPN 400
    SRFTAPAAQC PSIDPEWDNP AGVAIDAFIF GGRRSTTVPL VTEARDWTEG 450
    VYMAATMGSE TTAAAVGQQG VVRRDPFAML PFCGYNMADY FAHWLKLGDQ 500
    LAKSGAELPK IFCVNWFRKD EQGRFVWPGF GENMRVLKWM IDRIEGQARG 550
    DEHVFGVSPR YEELRWDGLD FSAEQFAKVI SLDAQAWQQE LTLHAELFAQ 600
    LAHHLPQALP EAKARLEARL QG 622
    Length:622
    Mass (Da):68,462
    Last modified:March 1, 2002 - v1
    Checksum:i6E6E16F6E90A61FE
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AL646052 Genomic DNA. Translation: CAD13545.1.
    RefSeqiNP_518138.1. NC_003295.1.
    WP_010999984.1. NC_003295.1.

    Genome annotation databases

    EnsemblBacteriaiCAD13545; CAD13545; RSc0017.
    GeneIDi1218819.
    KEGGirso:RSc0017.
    PATRICi20258565. VBIRalSol70888_0018.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AL646052 Genomic DNA. Translation: CAD13545.1 .
    RefSeqi NP_518138.1. NC_003295.1.
    WP_010999984.1. NC_003295.1.

    3D structure databases

    ProteinModelPortali Q8Y3G3.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    STRINGi 267608.RSc0017.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblBacteriai CAD13545 ; CAD13545 ; RSc0017 .
    GeneIDi 1218819.
    KEGGi rso:RSc0017.
    PATRICi 20258565. VBIRalSol70888_0018.

    Phylogenomic databases

    eggNOGi COG1274.
    HOGENOMi HOG000191700.
    KOi K01596.
    OMAi WDNPAGV.
    OrthoDBi EOG6MPWQS.

    Enzyme and pathway databases

    UniPathwayi UPA00138 .
    BioCyci RSOL267608:GCVU-17-MONOMER.

    Family and domain databases

    Gene3Di 3.40.449.10. 1 hit.
    3.90.228.20. 2 hits.
    HAMAPi MF_00452. PEPCK_GTP.
    InterProi IPR018091. PEP_carboxykin_GTP_CS.
    IPR013035. PEP_carboxykinase_C.
    IPR008209. PEP_carboxykinase_GTP.
    IPR008210. PEP_carboxykinase_N.
    [Graphical view ]
    PANTHERi PTHR11561. PTHR11561. 1 hit.
    Pfami PF00821. PEPCK. 1 hit.
    [Graphical view ]
    PIRSFi PIRSF001348. PEP_carboxykinase_GTP. 1 hit.
    SUPFAMi SSF68923. SSF68923. 1 hit.
    PROSITEi PS00505. PEPCK_GTP. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: GMI1000.

    Entry informationi

    Entry nameiPCKG_RALSO
    AccessioniPrimary (citable) accession number: Q8Y3G3
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: May 27, 2002
    Last sequence update: March 1, 2002
    Last modified: October 1, 2014
    This is version 78 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programProkaryotic Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. PATHWAY comments
      Index of metabolic and biosynthesis pathways
    2. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3