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Q8Y304

- GLMU_RALSO

UniProt

Q8Y304 - GLMU_RALSO

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Protein

Bifunctional protein GlmU

Gene

glmU

Organism
Ralstonia solanacearum (strain GMI1000) (Pseudomonas solanacearum)
Status
Reviewed - Annotation score: 4 out of 5- Protein inferred from homologyi

Functioni

Catalyzes the last two sequential reactions in the de novo biosynthetic pathway for UDP-N-acetylglucosamine (UDP-GlcNAc). The C-terminal domain catalyzes the transfer of acetyl group from acetyl coenzyme A to glucosamine-1-phosphate (GlcN-1-P) to produce N-acetylglucosamine-1-phosphate (GlcNAc-1-P), which is converted into UDP-GlcNAc by the transfer of uridine 5-monophosphate (from uridine 5-triphosphate), a reaction catalyzed by the N-terminal domain.UniRule annotation

Catalytic activityi

Acetyl-CoA + alpha-D-glucosamine 1-phosphate = CoA + N-acetyl-alpha-D-glucosamine 1-phosphate.UniRule annotation
UTP + N-acetyl-alpha-D-glucosamine 1-phosphate = diphosphate + UDP-N-acetyl-alpha-D-glucosamine.UniRule annotation

Cofactori

Binds 1 magnesium ion per subunit.UniRule annotation

Pathwayi

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei20 – 201UDP-GlcNAcUniRule annotation
Binding sitei71 – 711UDP-GlcNAcUniRule annotation
Metal bindingi100 – 1001MagnesiumUniRule annotation
Binding sitei135 – 1351UDP-GlcNAc; via amide nitrogenUniRule annotation
Binding sitei150 – 1501UDP-GlcNAcUniRule annotation
Binding sitei165 – 1651UDP-GlcNAcUniRule annotation
Metal bindingi223 – 2231MagnesiumUniRule annotation
Binding sitei223 – 2231UDP-GlcNAcUniRule annotation
Binding sitei329 – 3291Acetyl-CoA; amide nitrogenUniRule annotation
Binding sitei347 – 3471Acetyl-CoAUniRule annotation
Active sitei359 – 3591Proton acceptorUniRule annotation
Binding sitei362 – 3621Acetyl-CoAUniRule annotation
Binding sitei373 – 3731Acetyl-CoAUniRule annotation
Binding sitei401 – 4011Acetyl-CoAUniRule annotation
Binding sitei419 – 4191Acetyl-CoA; via amide nitrogenUniRule annotation
Binding sitei436 – 4361Acetyl-CoAUniRule annotation

GO - Molecular functioni

  1. glucosamine-1-phosphate N-acetyltransferase activity Source: UniProtKB-HAMAP
  2. magnesium ion binding Source: UniProtKB-HAMAP
  3. UDP-N-acetylglucosamine diphosphorylase activity Source: UniProtKB-HAMAP

GO - Biological processi

  1. cell morphogenesis Source: UniProtKB-HAMAP
  2. cell wall organization Source: UniProtKB-KW
  3. lipid A biosynthetic process Source: UniProtKB-UniPathway
  4. lipopolysaccharide biosynthetic process Source: InterPro
  5. peptidoglycan biosynthetic process Source: UniProtKB-HAMAP
  6. regulation of cell shape Source: UniProtKB-KW
  7. UDP-N-acetylglucosamine biosynthetic process Source: UniProtKB-UniPathway
Complete GO annotation...

Keywords - Molecular functioni

Acyltransferase, Nucleotidyltransferase, Transferase

Keywords - Biological processi

Cell shape, Cell wall biogenesis/degradation, Peptidoglycan synthesis

Keywords - Ligandi

Magnesium, Metal-binding

Enzyme and pathway databases

BioCyciRSOL267608:GCVU-178-MONOMER.
UniPathwayiUPA00113; UER00532.
UPA00113; UER00533.
UPA00973.

Names & Taxonomyi

Protein namesi
Recommended name:
Bifunctional protein GlmUUniRule annotation
Including the following 2 domains:
UDP-N-acetylglucosamine pyrophosphorylaseUniRule annotation (EC:2.7.7.23UniRule annotation)
Alternative name(s):
N-acetylglucosamine-1-phosphate uridyltransferaseUniRule annotation
Glucosamine-1-phosphate N-acetyltransferaseUniRule annotation (EC:2.3.1.157UniRule annotation)
Gene namesi
Name:glmUUniRule annotation
Ordered Locus Names:RSc0177
ORF Names:RS01048
OrganismiRalstonia solanacearum (strain GMI1000) (Pseudomonas solanacearum)
Taxonomic identifieri267608 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaBetaproteobacteriaBurkholderialesBurkholderiaceaeRalstonia
ProteomesiUP000001436: Chromosome

Subcellular locationi

Cytoplasm UniRule annotation

GO - Cellular componenti

  1. cytoplasm Source: UniProtKB-HAMAP
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 455455Bifunctional protein GlmUPRO_0000233830Add
BLAST

Interactioni

Subunit structurei

Homotrimer.UniRule annotation

Protein-protein interaction databases

STRINGi267608.RSc0177.

Structurei

3D structure databases

ProteinModelPortaliQ8Y304.
SMRiQ8Y304. Positions 1-447.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni1 – 225225PyrophosphorylaseUniRule annotationAdd
BLAST
Regioni6 – 94UDP-GlcNAc bindingUniRule annotation
Regioni76 – 772UDP-GlcNAc bindingUniRule annotation
Regioni98 – 1003UDP-GlcNAc bindingUniRule annotation
Regioni226 – 24621LinkerUniRule annotationAdd
BLAST
Regioni247 – 455209N-acetyltransferaseUniRule annotationAdd
BLAST
Regioni382 – 3832Acetyl-CoA bindingUniRule annotation

Sequence similaritiesi

In the N-terminal section; belongs to the N-acetylglucosamine-1-phosphate uridyltransferase family.UniRule annotation
In the C-terminal section; belongs to the transferase hexapeptide repeat family.UniRule annotation

Keywords - Domaini

Repeat

Phylogenomic databases

eggNOGiCOG1207.
HOGENOMiHOG000283476.
KOiK04042.
OMAiDCVTNQD.
OrthoDBiEOG6Z6FQZ.

Family and domain databases

Gene3Di3.90.550.10. 1 hit.
HAMAPiMF_01631. GlmU.
InterProiIPR005882. Bifunctional_GlmU.
IPR001451. Hexapep_transf.
IPR025877. MobA-like_NTP_Trfase_dom.
IPR029044. Nucleotide-diphossugar_trans.
IPR011004. Trimer_LpxA-like.
[Graphical view]
PfamiPF00132. Hexapep. 3 hits.
PF12804. NTP_transf_3. 1 hit.
[Graphical view]
SUPFAMiSSF51161. SSF51161. 1 hit.
SSF53448. SSF53448. 1 hit.
TIGRFAMsiTIGR01173. glmU. 1 hit.

Sequencei

Sequence statusi: Complete.

Q8Y304-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MNIVILAAGL GKRMRSALPK VLHPLAGKPL LAHVIETARS LSPTRLVVVV
60 70 80 90 100
GHGGDRVRDM VGAPDVTFAT QDQQLGTGHA VMQALDQLDD TVPTLVLYGD
110 120 130 140 150
VPLTRAETLN ALVGAAGQDH LGVLTVHLDD PTGYGRIVRD ATGRITRIVE
160 170 180 190 200
QKDANETQLA IHEVNTGILV CPTARLKTWL ATLRNDNAQG EYYLTDVIER
210 220 230 240 250
AASEGLPITS AHPLAEWETL GVNSKVQLAE LERIHQRNLA QQLLEDGVTL
260 270 280 290 300
IDPARIDIRG RLTCGRDVVI DIDCIFEGNV TLGDGVRIGA HAVIRDAAIQ
310 320 330 340 350
AGAEILPFCH IEQATVGAQS RIGPYARLRP GTELAEDVHI GNFVEVKNSQ
360 370 380 390 400
IAAHSKANHL AYVGDATVGS RVNIGAGTIT CNYDGANKFR TIIEDDAFIG
410 420 430 440 450
SDTQLVAPVR VGRGATLGAG TTLTKDAPEG QLTVSRARQT TVNGWQRPVK

QKKDA
Length:455
Mass (Da):48,670
Last modified:March 1, 2002 - v1
Checksum:iD7A3647EA8320BD4
GO

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AL646052 Genomic DNA. Translation: CAD13705.1.
RefSeqiNP_518298.1. NC_003295.1.

Genome annotation databases

EnsemblBacteriaiCAD13705; CAD13705; RSc0177.
GeneIDi1218980.
KEGGirso:RSc0177.
PATRICi20258893. VBIRalSol70888_0181.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AL646052 Genomic DNA. Translation: CAD13705.1 .
RefSeqi NP_518298.1. NC_003295.1.

3D structure databases

ProteinModelPortali Q8Y304.
SMRi Q8Y304. Positions 1-447.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

STRINGi 267608.RSc0177.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblBacteriai CAD13705 ; CAD13705 ; RSc0177 .
GeneIDi 1218980.
KEGGi rso:RSc0177.
PATRICi 20258893. VBIRalSol70888_0181.

Phylogenomic databases

eggNOGi COG1207.
HOGENOMi HOG000283476.
KOi K04042.
OMAi DCVTNQD.
OrthoDBi EOG6Z6FQZ.

Enzyme and pathway databases

UniPathwayi UPA00113 ; UER00532 .
UPA00113 ; UER00533 .
UPA00973 .
BioCyci RSOL267608:GCVU-178-MONOMER.

Family and domain databases

Gene3Di 3.90.550.10. 1 hit.
HAMAPi MF_01631. GlmU.
InterProi IPR005882. Bifunctional_GlmU.
IPR001451. Hexapep_transf.
IPR025877. MobA-like_NTP_Trfase_dom.
IPR029044. Nucleotide-diphossugar_trans.
IPR011004. Trimer_LpxA-like.
[Graphical view ]
Pfami PF00132. Hexapep. 3 hits.
PF12804. NTP_transf_3. 1 hit.
[Graphical view ]
SUPFAMi SSF51161. SSF51161. 1 hit.
SSF53448. SSF53448. 1 hit.
TIGRFAMsi TIGR01173. glmU. 1 hit.
ProtoNeti Search...

Publicationsi

  1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: GMI1000.

Entry informationi

Entry nameiGLMU_RALSO
AccessioniPrimary (citable) accession number: Q8Y304
Entry historyi
Integrated into UniProtKB/Swiss-Prot: May 2, 2006
Last sequence update: March 1, 2002
Last modified: October 29, 2014
This is version 79 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Multifunctional enzyme, Reference proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3