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Q8Y304

- GLMU_RALSO

UniProt

Q8Y304 - GLMU_RALSO

Protein

Bifunctional protein GlmU

Gene

glmU

Organism
Ralstonia solanacearum (strain GMI1000) (Pseudomonas solanacearum)
Status
Reviewed - Annotation score: 4 out of 5- Protein inferred from homologyi
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    • History
      Entry version 78 (01 Oct 2014)
      Sequence version 1 (01 Mar 2002)
      Previous versions | rss
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    Functioni

    Catalyzes the last two sequential reactions in the de novo biosynthetic pathway for UDP-N-acetylglucosamine (UDP-GlcNAc). The C-terminal domain catalyzes the transfer of acetyl group from acetyl coenzyme A to glucosamine-1-phosphate (GlcN-1-P) to produce N-acetylglucosamine-1-phosphate (GlcNAc-1-P), which is converted into UDP-GlcNAc by the transfer of uridine 5-monophosphate (from uridine 5-triphosphate), a reaction catalyzed by the N-terminal domain.UniRule annotation

    Catalytic activityi

    Acetyl-CoA + alpha-D-glucosamine 1-phosphate = CoA + N-acetyl-alpha-D-glucosamine 1-phosphate.UniRule annotation
    UTP + N-acetyl-alpha-D-glucosamine 1-phosphate = diphosphate + UDP-N-acetyl-alpha-D-glucosamine.UniRule annotation

    Cofactori

    Binds 1 magnesium ion per subunit.UniRule annotation

    Pathwayi

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Binding sitei20 – 201UDP-GlcNAcUniRule annotation
    Binding sitei71 – 711UDP-GlcNAcUniRule annotation
    Metal bindingi100 – 1001MagnesiumUniRule annotation
    Binding sitei135 – 1351UDP-GlcNAc; via amide nitrogenUniRule annotation
    Binding sitei150 – 1501UDP-GlcNAcUniRule annotation
    Binding sitei165 – 1651UDP-GlcNAcUniRule annotation
    Metal bindingi223 – 2231MagnesiumUniRule annotation
    Binding sitei223 – 2231UDP-GlcNAcUniRule annotation
    Binding sitei329 – 3291Acetyl-CoA; amide nitrogenUniRule annotation
    Binding sitei347 – 3471Acetyl-CoAUniRule annotation
    Active sitei359 – 3591Proton acceptorUniRule annotation
    Binding sitei362 – 3621Acetyl-CoAUniRule annotation
    Binding sitei373 – 3731Acetyl-CoAUniRule annotation
    Binding sitei401 – 4011Acetyl-CoAUniRule annotation
    Binding sitei419 – 4191Acetyl-CoA; via amide nitrogenUniRule annotation
    Binding sitei436 – 4361Acetyl-CoAUniRule annotation

    GO - Molecular functioni

    1. glucosamine-1-phosphate N-acetyltransferase activity Source: UniProtKB-HAMAP
    2. magnesium ion binding Source: UniProtKB-HAMAP
    3. UDP-N-acetylglucosamine diphosphorylase activity Source: UniProtKB-HAMAP

    GO - Biological processi

    1. cell morphogenesis Source: UniProtKB-HAMAP
    2. lipid A biosynthetic process Source: UniProtKB-UniPathway
    3. lipopolysaccharide biosynthetic process Source: InterPro
    4. peptidoglycan biosynthetic process Source: UniProtKB-HAMAP
    5. regulation of cell shape Source: UniProtKB-KW
    6. UDP-N-acetylglucosamine biosynthetic process Source: UniProtKB-UniPathway

    Keywords - Molecular functioni

    Acyltransferase, Nucleotidyltransferase, Transferase

    Keywords - Biological processi

    Cell shape, Cell wall biogenesis/degradation, Peptidoglycan synthesis

    Keywords - Ligandi

    Magnesium, Metal-binding

    Enzyme and pathway databases

    BioCyciRSOL267608:GCVU-178-MONOMER.
    UniPathwayiUPA00113; UER00532.
    UPA00113; UER00533.
    UPA00973.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Bifunctional protein GlmUUniRule annotation
    Including the following 2 domains:
    UDP-N-acetylglucosamine pyrophosphorylaseUniRule annotation (EC:2.7.7.23UniRule annotation)
    Alternative name(s):
    N-acetylglucosamine-1-phosphate uridyltransferaseUniRule annotation
    Glucosamine-1-phosphate N-acetyltransferaseUniRule annotation (EC:2.3.1.157UniRule annotation)
    Gene namesi
    Name:glmUUniRule annotation
    Ordered Locus Names:RSc0177
    ORF Names:RS01048
    OrganismiRalstonia solanacearum (strain GMI1000) (Pseudomonas solanacearum)
    Taxonomic identifieri267608 [NCBI]
    Taxonomic lineageiBacteriaProteobacteriaBetaproteobacteriaBurkholderialesBurkholderiaceaeRalstonia
    ProteomesiUP000001436: Chromosome

    Subcellular locationi

    Cytoplasm UniRule annotation

    GO - Cellular componenti

    1. cytoplasm Source: UniProtKB-SubCell

    Keywords - Cellular componenti

    Cytoplasm

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 455455Bifunctional protein GlmUPRO_0000233830Add
    BLAST

    Interactioni

    Subunit structurei

    Homotrimer.UniRule annotation

    Protein-protein interaction databases

    STRINGi267608.RSc0177.

    Structurei

    3D structure databases

    ProteinModelPortaliQ8Y304.
    SMRiQ8Y304. Positions 1-447.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Region

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Regioni1 – 225225PyrophosphorylaseUniRule annotationAdd
    BLAST
    Regioni6 – 94UDP-GlcNAc bindingUniRule annotation
    Regioni76 – 772UDP-GlcNAc bindingUniRule annotation
    Regioni98 – 1003UDP-GlcNAc bindingUniRule annotation
    Regioni226 – 24621LinkerUniRule annotationAdd
    BLAST
    Regioni247 – 455209N-acetyltransferaseUniRule annotationAdd
    BLAST
    Regioni382 – 3832Acetyl-CoA bindingUniRule annotation

    Sequence similaritiesi

    In the N-terminal section; belongs to the N-acetylglucosamine-1-phosphate uridyltransferase family.UniRule annotation
    In the C-terminal section; belongs to the transferase hexapeptide repeat family.UniRule annotation

    Keywords - Domaini

    Repeat

    Phylogenomic databases

    eggNOGiCOG1207.
    HOGENOMiHOG000283476.
    KOiK04042.
    OMAiDCVTNQD.
    OrthoDBiEOG6Z6FQZ.

    Family and domain databases

    Gene3Di3.90.550.10. 1 hit.
    HAMAPiMF_01631. GlmU.
    InterProiIPR005882. Bifunctional_GlmU.
    IPR001451. Hexapep_transf.
    IPR025877. MobA-like_NTP_Trfase_dom.
    IPR029044. Nucleotide-diphossugar_trans.
    IPR011004. Trimer_LpxA-like.
    [Graphical view]
    PfamiPF00132. Hexapep. 3 hits.
    PF12804. NTP_transf_3. 1 hit.
    [Graphical view]
    SUPFAMiSSF51161. SSF51161. 1 hit.
    SSF53448. SSF53448. 1 hit.
    TIGRFAMsiTIGR01173. glmU. 1 hit.

    Sequencei

    Sequence statusi: Complete.

    Q8Y304-1 [UniParc]FASTAAdd to Basket

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    MNIVILAAGL GKRMRSALPK VLHPLAGKPL LAHVIETARS LSPTRLVVVV    50
    GHGGDRVRDM VGAPDVTFAT QDQQLGTGHA VMQALDQLDD TVPTLVLYGD 100
    VPLTRAETLN ALVGAAGQDH LGVLTVHLDD PTGYGRIVRD ATGRITRIVE 150
    QKDANETQLA IHEVNTGILV CPTARLKTWL ATLRNDNAQG EYYLTDVIER 200
    AASEGLPITS AHPLAEWETL GVNSKVQLAE LERIHQRNLA QQLLEDGVTL 250
    IDPARIDIRG RLTCGRDVVI DIDCIFEGNV TLGDGVRIGA HAVIRDAAIQ 300
    AGAEILPFCH IEQATVGAQS RIGPYARLRP GTELAEDVHI GNFVEVKNSQ 350
    IAAHSKANHL AYVGDATVGS RVNIGAGTIT CNYDGANKFR TIIEDDAFIG 400
    SDTQLVAPVR VGRGATLGAG TTLTKDAPEG QLTVSRARQT TVNGWQRPVK 450
    QKKDA 455
    Length:455
    Mass (Da):48,670
    Last modified:March 1, 2002 - v1
    Checksum:iD7A3647EA8320BD4
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AL646052 Genomic DNA. Translation: CAD13705.1.
    RefSeqiNP_518298.1. NC_003295.1.

    Genome annotation databases

    EnsemblBacteriaiCAD13705; CAD13705; RSc0177.
    GeneIDi1218980.
    KEGGirso:RSc0177.
    PATRICi20258893. VBIRalSol70888_0181.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AL646052 Genomic DNA. Translation: CAD13705.1 .
    RefSeqi NP_518298.1. NC_003295.1.

    3D structure databases

    ProteinModelPortali Q8Y304.
    SMRi Q8Y304. Positions 1-447.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    STRINGi 267608.RSc0177.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblBacteriai CAD13705 ; CAD13705 ; RSc0177 .
    GeneIDi 1218980.
    KEGGi rso:RSc0177.
    PATRICi 20258893. VBIRalSol70888_0181.

    Phylogenomic databases

    eggNOGi COG1207.
    HOGENOMi HOG000283476.
    KOi K04042.
    OMAi DCVTNQD.
    OrthoDBi EOG6Z6FQZ.

    Enzyme and pathway databases

    UniPathwayi UPA00113 ; UER00532 .
    UPA00113 ; UER00533 .
    UPA00973 .
    BioCyci RSOL267608:GCVU-178-MONOMER.

    Family and domain databases

    Gene3Di 3.90.550.10. 1 hit.
    HAMAPi MF_01631. GlmU.
    InterProi IPR005882. Bifunctional_GlmU.
    IPR001451. Hexapep_transf.
    IPR025877. MobA-like_NTP_Trfase_dom.
    IPR029044. Nucleotide-diphossugar_trans.
    IPR011004. Trimer_LpxA-like.
    [Graphical view ]
    Pfami PF00132. Hexapep. 3 hits.
    PF12804. NTP_transf_3. 1 hit.
    [Graphical view ]
    SUPFAMi SSF51161. SSF51161. 1 hit.
    SSF53448. SSF53448. 1 hit.
    TIGRFAMsi TIGR01173. glmU. 1 hit.
    ProtoNeti Search...

    Publicationsi

    1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: GMI1000.

    Entry informationi

    Entry nameiGLMU_RALSO
    AccessioniPrimary (citable) accession number: Q8Y304
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: May 2, 2006
    Last sequence update: March 1, 2002
    Last modified: October 1, 2014
    This is version 78 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programProkaryotic Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Multifunctional enzyme, Reference proteome

    Documents

    1. PATHWAY comments
      Index of metabolic and biosynthesis pathways
    2. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3