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Q8Y304

- GLMU_RALSO

UniProt

Q8Y304 - GLMU_RALSO

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Protein
Bifunctional protein GlmU
Gene
glmU, RSc0177, RS01048
Organism
Ralstonia solanacearum (strain GMI1000) (Pseudomonas solanacearum)
Status
Reviewed - Annotation score: 4 out of 5 - Protein inferred from homologyi

Functioni

Catalyzes the last two sequential reactions in the de novo biosynthetic pathway for UDP-N-acetylglucosamine (UDP-GlcNAc). The C-terminal domain catalyzes the transfer of acetyl group from acetyl coenzyme A to glucosamine-1-phosphate (GlcN-1-P) to produce N-acetylglucosamine-1-phosphate (GlcNAc-1-P), which is converted into UDP-GlcNAc by the transfer of uridine 5-monophosphate (from uridine 5-triphosphate), a reaction catalyzed by the N-terminal domain By similarity.UniRule annotation

Catalytic activityi

Acetyl-CoA + alpha-D-glucosamine 1-phosphate = CoA + N-acetyl-alpha-D-glucosamine 1-phosphate.UniRule annotation
UTP + N-acetyl-alpha-D-glucosamine 1-phosphate = diphosphate + UDP-N-acetyl-alpha-D-glucosamine.UniRule annotation

Cofactori

Binds 1 magnesium ion per subunit By similarity.UniRule annotation

Pathwayi

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei20 – 201UDP-GlcNAc By similarity
Binding sitei71 – 711UDP-GlcNAc By similarity
Metal bindingi100 – 1001Magnesium By similarity
Binding sitei135 – 1351UDP-GlcNAc; via amide nitrogen By similarity
Binding sitei150 – 1501UDP-GlcNAc By similarity
Binding sitei165 – 1651UDP-GlcNAc By similarity
Metal bindingi223 – 2231Magnesium By similarity
Binding sitei223 – 2231UDP-GlcNAc By similarity
Binding sitei329 – 3291Acetyl-CoA; amide nitrogen By similarity
Binding sitei347 – 3471Acetyl-CoA By similarity
Active sitei359 – 3591Proton acceptor By similarity
Binding sitei362 – 3621Acetyl-CoA By similarity
Binding sitei373 – 3731Acetyl-CoA By similarity
Binding sitei401 – 4011Acetyl-CoA By similarity
Binding sitei419 – 4191Acetyl-CoA; via amide nitrogen By similarity
Binding sitei436 – 4361Acetyl-CoA By similarity

GO - Molecular functioni

  1. UDP-N-acetylglucosamine diphosphorylase activity Source: UniProtKB-HAMAP
  2. glucosamine-1-phosphate N-acetyltransferase activity Source: UniProtKB-HAMAP
  3. magnesium ion binding Source: UniProtKB-HAMAP

GO - Biological processi

  1. UDP-N-acetylglucosamine biosynthetic process Source: UniProtKB-UniPathway
  2. cell morphogenesis Source: UniProtKB-HAMAP
  3. lipid A biosynthetic process Source: UniProtKB-UniPathway
  4. lipopolysaccharide biosynthetic process Source: InterPro
  5. peptidoglycan biosynthetic process Source: UniProtKB-HAMAP
  6. regulation of cell shape Source: UniProtKB-KW
Complete GO annotation...

Keywords - Molecular functioni

Acyltransferase, Nucleotidyltransferase, Transferase

Keywords - Biological processi

Cell shape, Cell wall biogenesis/degradation, Peptidoglycan synthesis

Keywords - Ligandi

Magnesium, Metal-binding

Enzyme and pathway databases

BioCyciRSOL267608:GCVU-178-MONOMER.
UniPathwayiUPA00113; UER00532.
UPA00113; UER00533.
UPA00973.

Names & Taxonomyi

Protein namesi
Recommended name:
Bifunctional protein GlmU
Including the following 2 domains:
UDP-N-acetylglucosamine pyrophosphorylase (EC:2.7.7.23)
Alternative name(s):
N-acetylglucosamine-1-phosphate uridyltransferase
Glucosamine-1-phosphate N-acetyltransferase (EC:2.3.1.157)
Gene namesi
Name:glmU
Ordered Locus Names:RSc0177
ORF Names:RS01048
OrganismiRalstonia solanacearum (strain GMI1000) (Pseudomonas solanacearum)
Taxonomic identifieri267608 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaBetaproteobacteriaBurkholderialesBurkholderiaceaeRalstonia
ProteomesiUP000001436: Chromosome

Subcellular locationi

Cytoplasm By similarity UniRule annotation

GO - Cellular componenti

  1. cytoplasm Source: UniProtKB-SubCell
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 455455Bifunctional protein GlmUUniRule annotation
PRO_0000233830Add
BLAST

Interactioni

Subunit structurei

Homotrimer By similarity.UniRule annotation

Protein-protein interaction databases

STRINGi267608.RSc0177.

Structurei

3D structure databases

ProteinModelPortaliQ8Y304.
SMRiQ8Y304. Positions 1-447.

Family & Domainsi

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni1 – 225225Pyrophosphorylase By similarity
Add
BLAST
Regioni6 – 94UDP-GlcNAc binding By similarity
Regioni76 – 772UDP-GlcNAc binding By similarity
Regioni98 – 1003UDP-GlcNAc binding By similarity
Regioni226 – 24621Linker By similarity
Add
BLAST
Regioni247 – 455209N-acetyltransferase By similarity
Add
BLAST
Regioni382 – 3832Acetyl-CoA binding By similarity

Sequence similaritiesi

In the N-terminal section; belongs to the N-acetylglucosamine-1-phosphate uridyltransferase family.
In the C-terminal section; belongs to the transferase hexapeptide repeat family.

Keywords - Domaini

Repeat

Phylogenomic databases

eggNOGiCOG1207.
HOGENOMiHOG000283476.
KOiK04042.
OMAiDCVTNQD.
OrthoDBiEOG6Z6FQZ.

Family and domain databases

Gene3Di3.90.550.10. 1 hit.
HAMAPiMF_01631. GlmU.
InterProiIPR005882. Bifunctional_GlmU.
IPR001451. Hexapep_transf.
IPR025877. MobA-like_NTP_Trfase_dom.
IPR029044. Nucleotide-diphossugar_trans.
IPR011004. Trimer_LpxA-like.
[Graphical view]
PfamiPF00132. Hexapep. 3 hits.
PF12804. NTP_transf_3. 1 hit.
[Graphical view]
SUPFAMiSSF51161. SSF51161. 1 hit.
SSF53448. SSF53448. 1 hit.
TIGRFAMsiTIGR01173. glmU. 1 hit.

Sequencei

Sequence statusi: Complete.

Q8Y304-1 [UniParc]FASTAAdd to Basket

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MNIVILAAGL GKRMRSALPK VLHPLAGKPL LAHVIETARS LSPTRLVVVV    50
GHGGDRVRDM VGAPDVTFAT QDQQLGTGHA VMQALDQLDD TVPTLVLYGD 100
VPLTRAETLN ALVGAAGQDH LGVLTVHLDD PTGYGRIVRD ATGRITRIVE 150
QKDANETQLA IHEVNTGILV CPTARLKTWL ATLRNDNAQG EYYLTDVIER 200
AASEGLPITS AHPLAEWETL GVNSKVQLAE LERIHQRNLA QQLLEDGVTL 250
IDPARIDIRG RLTCGRDVVI DIDCIFEGNV TLGDGVRIGA HAVIRDAAIQ 300
AGAEILPFCH IEQATVGAQS RIGPYARLRP GTELAEDVHI GNFVEVKNSQ 350
IAAHSKANHL AYVGDATVGS RVNIGAGTIT CNYDGANKFR TIIEDDAFIG 400
SDTQLVAPVR VGRGATLGAG TTLTKDAPEG QLTVSRARQT TVNGWQRPVK 450
QKKDA 455
Length:455
Mass (Da):48,670
Last modified:March 1, 2002 - v1
Checksum:iD7A3647EA8320BD4
GO

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AL646052 Genomic DNA. Translation: CAD13705.1.
RefSeqiNP_518298.1. NC_003295.1.

Genome annotation databases

EnsemblBacteriaiCAD13705; CAD13705; RSc0177.
GeneIDi1218980.
KEGGirso:RSc0177.
PATRICi20258893. VBIRalSol70888_0181.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AL646052 Genomic DNA. Translation: CAD13705.1 .
RefSeqi NP_518298.1. NC_003295.1.

3D structure databases

ProteinModelPortali Q8Y304.
SMRi Q8Y304. Positions 1-447.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

STRINGi 267608.RSc0177.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblBacteriai CAD13705 ; CAD13705 ; RSc0177 .
GeneIDi 1218980.
KEGGi rso:RSc0177.
PATRICi 20258893. VBIRalSol70888_0181.

Phylogenomic databases

eggNOGi COG1207.
HOGENOMi HOG000283476.
KOi K04042.
OMAi DCVTNQD.
OrthoDBi EOG6Z6FQZ.

Enzyme and pathway databases

UniPathwayi UPA00113 ; UER00532 .
UPA00113 ; UER00533 .
UPA00973 .
BioCyci RSOL267608:GCVU-178-MONOMER.

Family and domain databases

Gene3Di 3.90.550.10. 1 hit.
HAMAPi MF_01631. GlmU.
InterProi IPR005882. Bifunctional_GlmU.
IPR001451. Hexapep_transf.
IPR025877. MobA-like_NTP_Trfase_dom.
IPR029044. Nucleotide-diphossugar_trans.
IPR011004. Trimer_LpxA-like.
[Graphical view ]
Pfami PF00132. Hexapep. 3 hits.
PF12804. NTP_transf_3. 1 hit.
[Graphical view ]
SUPFAMi SSF51161. SSF51161. 1 hit.
SSF53448. SSF53448. 1 hit.
TIGRFAMsi TIGR01173. glmU. 1 hit.
ProtoNeti Search...

Publicationsi

  1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: GMI1000.

Entry informationi

Entry nameiGLMU_RALSO
AccessioniPrimary (citable) accession number: Q8Y304
Entry historyi
Integrated into UniProtKB/Swiss-Prot: May 2, 2006
Last sequence update: March 1, 2002
Last modified: June 11, 2014
This is version 77 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Multifunctional enzyme

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

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