Reviewed, UniProtKB/Swiss-Prot Q8Y2V6 (RTCA_RALSO)

Names and origin

Protein names

Recommended name:
    Probable RNA 3'-terminal phosphate cyclase
      Short name=RNA-3'-phosphate cyclase
      Short name=RNA cyclase
    EC=6.5.1.4

Gene names

Name:	rtcA
Ordered Locus Names:	RSc0226
ORF Names:	RS00658

Organism

Ralstonia solanacearum (Pseudomonas solanacearum) [Complete proteome] [HAMAP]

Taxonomic identifier

305 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Betaproteobacteria › Burkholderiales › Burkholderiaceae › Ralstonia

Protein attributes

Sequence length	347 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Inferred from homology.

General annotation (Comments)

Function	Catalyzes the conversion of 3'-phosphate to a 2',3'-cyclic phosphodiester at the end of RNA. The mechanism of action of the enzyme occurs in 3 steps: (A) adenylation of the enzyme by ATP; (B) the enzyme acts on RNA-N3'P to produce RNA-N3'PP5'A; (C) a non catalytic nucleophilic attack by the adjacent 2'hydroxyl on the phosphorus in the diester linkage to produce the cyclic end product. The biological role of this enzyme is unknown but it is likely to function in some aspects of cellular RNA processing By similarity.
Catalytic activity	ATP + RNA 3'-terminal-phosphate = AMP + diphosphate + RNA terminal-2',3'-cyclic-phosphate. HAMAP MF_00200
Subcellular location	Cytoplasm Potential.
Sequence similarities	Belongs to the RNA 3'-terminal cyclase family. Type 1 subfamily.

Ontologies

Keywords
Cellular component	Cytoplasm
Ligand	ATP-binding Nucleotide-binding
Molecular function	Ligase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	RNA processing Inferred from electronic annotation. Source: InterPro
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW RNA-3'-phosphate cyclase activity Inferred from electronic annotation. Source: HAMAP
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 347	347	Probable RNA 3'-terminal phosphate cyclase HAMAP MF_00200		PRO_0000156420
Sites
	Active site	315	1	By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q8Y2V6-1 [UniParc]. Last modified March 1, 2002. Version 1. Checksum: 913E69C707B70524	FASTA	347	35,970
	10 20 30 40 50 60 MNNRLQPAPC IELDGAQGEG GGQILRTALT LSMLTGTPFR IERIRAGRSK PGLMRQHLTA 70 80 90 100 110 120 VQAAAEVSGA TVEGAEAGSQ ALAFAPGPIH GGDYRFAIGT AGSCTLVLQT VLPALWFADA 130 140 150 160 170 180 PSTVAVSGGT HNRAAPPVDF LIRAWQPLLA RMGVTQTLAL KRHGFYPAGG GEVLATVTPC 190 200 210 220 230 240 AGRLGALHLT ERGALRELSG QGIVANVRPG VARRELEALA ARVPGVVGSV RELSPAEGPG 250 260 270 280 290 300 NALVLDAVHE HVTEVFTGFG ERGVPAEQVA HGVASAALRY LHSTAAVDEY LADQLVLPMA 310 320 330 340 LAGAGCFTAV TASPHLTTNI AVIEKFLPVH ITVQAERDAS VVRVQVN

References

[1]

"Genome sequence of the plant pathogen Ralstonia solanacearum." Salanoubat M., Genin S., Artiguenave F., Gouzy J., Mangenot S., Arlat M., Billault A., Brottier P., Camus J.-C., Cattolico L., Chandler M., Choisne N., Claudel-Renard C., Cunnac S., Demange N., Gaspin C., Lavie M., Moisan A. , Robert C., Saurin W., Schiex T., Siguier P., Thebault P., Whalen M., Wincker P., Levy M., Weissenbach J., Boucher C.A. Nature 415:497-502(2002) [PubMed: 11823852] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: GMI1000.

Cross-references

Sequence databases
EMBLGenBankDDBJ	AL646052 Genomic DNA. Translation: CAD13754.1.
RefSeq	NP_518347.1.
3D structure databases
HSSP	HSSP built from PDB template 1QMH based on UniProtKB P46849.
ModBase	Search...
Genome annotation databases
GeneID	1219029.
GenomeReviews	Gene locus RSc0226 in contig AL646052_GR.
KEGG	rso:RSc0226.
NMPDR	fig|267608.1.peg.226.
Organism-specific databases
CMR	Search...
Phylogenomic databases
HOGENOM	Q8Y2V6.
OMA	EIDGSYG.
Enzyme and pathway databases
BioCyc	RSOL267608:RSC0226-MON.
BRENDA	6.5.1.4. 97066.
Family and domain databases
HAMAP	MF_00200. [Tree]
InterPro	IPR000228. RNA3'_term_phos_cycl-like. IPR013796. RNA3'_term_phos_cycl_insert. IPR017770. RNA3'_term_phos_cycl_sub. [Graphical view]
Gene3D	G3DSA:3.65.10.20. RNA3'_term_phos_cycl. 1 hit.
PANTHER	PTHR11096. RNA3'_term_phos_cycl. 1 hit.
Pfam	PF01137. RTC. 1 hit. PF05189. RTC_insert. 1 hit. [Graphical view]
TIGRFAMs	TIGR03399. RNA_3prim_cycl. 1 hit.
PROSITE	PS01287. RTC. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

RTCA_RALSO

Accession

Primary (citable) accession number: Q8Y2V6

Entry history

Integrated into UniProtKB/Swiss-Prot:	May 27, 2002
Last sequence update:	March 1, 2002
Last modified:	November 3, 2009
This is version 44 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)