Reviewed, UniProtKB/Swiss-Prot Q8Y2Q7 (ACEK_RALSO)

Names and origin

Protein names

Recommended name:
    Isocitrate dehydrogenase kinase/phosphatase
      Short name=IDH kinase/phosphatase
      Short name=IDHK/P
    EC=2.7.11.5
    EC=3.1.3.-

Gene names

Name:	aceK
Ordered Locus Names:	RSc0278
ORF Names:	RS03245

Organism

Ralstonia solanacearum (Pseudomonas solanacearum) [Complete proteome] [HAMAP]

Taxonomic identifier

305 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Betaproteobacteria › Burkholderiales › Burkholderiaceae › Ralstonia

Protein attributes

Sequence length	608 AA.
Sequence status	Complete.
Protein existence	Inferred from homology.

General annotation (Comments)

Function	Bifunctional enzyme which can phosphorylate or dephosphorylate isocitrate dehydrogenase (IDH) on a specific serine residue. This is a regulatory mechanism which enables bacteria to bypass the Krebs cycle via the glyoxylate shunt in response to the source of carbon. When bacteria are grown on glucose, IDH is fully active and unphosphorylated, but when grown on acetate or ethanol, the activity of IDH declines drastically concomitant with its phosphorylation By similarity. HAMAP MF_00747
Catalytic activity	ATP + [isocitrate dehydrogenase (NADP+)] = ADP + [isocitrate dehydrogenase (NADP+)] phosphate. HAMAP MF_00747
Subcellular location	Cytoplasm By similarity HAMAP MF_00747.
Sequence similarities	Belongs to the aceK family.

Ontologies

Keywords
Biological process	Glyoxylate bypass Tricarboxylic acid cycle
Cellular component	Cytoplasm
Ligand	ATP-binding NADP Nucleotide-binding
Molecular function	Hydrolase Kinase Protein phosphatase Transferase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	glucose metabolic process Inferred from electronic annotation. Source: InterPro glyoxylate cycle Inferred from electronic annotation. Source: HAMAP tricarboxylic acid cycle Inferred from electronic annotation. Source: HAMAP
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	ATP binding Inferred from electronic annotation. Source: HAMAP [isocitrate dehydrogenase (NADP+)] kinase activity Inferred from electronic annotation. Source: HAMAP phosphoprotein phosphatase activity Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 608	608	Isocitrate dehydrogenase kinase/phosphatase HAMAP MF_00747		PRO_0000057906
Regions
	Nucleotide binding	328 – 334	7	ATP By similarity
Sites
	Active site	384	1	By similarity
	Binding site	349	1	ATP By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q8Y2Q7-1 [UniParc]. Last modified March 1, 2002. Version 1. Checksum: A7300AF83E114901	FASTA	608	70,553
	10 20 30 40 50 60 MSHFPKLLSS QIAFDVARTM LDGFDKHYRL FREVSVRAKA LFEAGDWQGL QQLQRDRIAY 70 80 90 100 110 120 YDERVREASV ILEDEYDAEN IDDEIWRQIK LHFIGLLTNH HQPECAETFF NSVCTRILHR 130 140 150 160 170 180 SYFNNDFIFL RPAISTEYIE NEESPTKPTY RAYYPGSREG LGGCLERIVH NFQLDAPFED 190 200 210 220 230 240 LPRDIGYVVR AVGEHFGDFR IAPNFQIHVL SSLFFRNTAA YIIGRVINGD KTYPLAVPIM 250 260 270 280 290 300 RGADGQLRLD TVLLRPEQLL ILFSFTHSYF MVDMEIPSAY VTFLRDLMPR KPRAEIYTSL 310 320 330 340 350 360 GLQKQGKNLF YRDFLHHLQH SSDKFIIAPG IKGLVMLVFT LPSYPYVFKV IKDYFPPPKE 370 380 390 400 410 420 TTRELIKSKY QLVKQHDRVG RMADTLEYSD VAFPLSRFDD ALIKELEKHA PSMVEYQRGA 430 440 450 460 470 480 DGSDEIVIRH VYIERRMTPL NIWLHEGTDA QVEHGIIEYG NAIKELIAAN IFPGDMLYKN 490 500 510 520 530 540 FGVTRHGRVV FYDYDEIEYL TDCNVRDVPQ PRNEEEEMSG EVWYSVGPHD IFPQTYGTFL 550 560 570 580 590 600 LGDPRVREAF LRHHADFFDP AMWQAHKARL LSGQTHDFYA YDASERFVNR YGDSAPGNAA TPLRRAAA

References

[1]

"Genome sequence of the plant pathogen Ralstonia solanacearum." Salanoubat M., Genin S., Artiguenave F., Gouzy J., Mangenot S., Arlat M., Billault A., Brottier P., Camus J.-C., Cattolico L., Chandler M., Choisne N., Claudel-Renard C., Cunnac S., Demange N., Gaspin C., Lavie M., Moisan A. , Robert C., Saurin W., Schiex T., Siguier P., Thebault P., Whalen M., Wincker P., Levy M., Weissenbach J., Boucher C.A. Nature 415:497-502(2002) [PubMed: 11823852] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: GMI1000.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AL646052 Genomic DNA. Translation: CAD13806.1.
RefSeq	NP_518399.1.
3D structure databases
ModBase	Search...
Genome annotation databases
GeneID	1219081.
GenomeReviews	Gene locus RSc0278 in contig AL646052_GR.
KEGG	rso:RSc0278.
NMPDR	fig|267608.1.peg.278.
Organism-specific databases
CMR	Search...
Phylogenomic databases
HOGENOM	HBG298593.
OMA	RMTPLNI.
Enzyme and pathway databases
BioCyc	RSOL267608:RSC0278-MONOMER.
BRENDA	2.7.11.5. 97066.
Family and domain databases
HAMAP	MF_00747. AceK. [Tree]
InterPro	IPR010452. Isocitrate_DH_AceK. [Graphical view]
Pfam	PF06315. AceK. 1 hit. [Graphical view]
PIRSF	PIRSF000719. AceK. 1 hit.
ProDom	PD043552. Isocitrate_DH_AceK. 1 hit. [Graphical view] [Entries sharing at least one domain]
ProtoNet	Search...

Entry information

Entry name

ACEK_RALSO

Accession

Primary (citable) accession number: Q8Y2Q7

Entry history

Integrated into UniProtKB/Swiss-Prot:	January 16, 2004
Last sequence update:	March 1, 2002
Last modified:	January 19, 2010
This is version 50 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)