Q8Y2P8 (SYR_RALSO) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Arginine--tRNA ligase
EC=6.1.1.19

Alternative name(s):
Arginyl-tRNA synthetase
Short name=ArgRS

Gene names

Name:	argS
Ordered Locus Names:	RSc0287
ORF Names:	RS03254

Organism

Ralstonia solanacearum (strain GMI1000) (Pseudomonas solanacearum) [Complete proteome] [HAMAP]

Taxonomic identifier

267608 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Betaproteobacteria › Burkholderiales › Burkholderiaceae › Ralstonia

Protein attributes

Sequence length	600 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Catalytic activity	ATP + L-arginine + tRNA(Arg) = AMP + diphosphate + L-arginyl-tRNA(Arg). HAMAP MF_00123
Subunit structure	Monomer By similarity. HAMAP MF_00123
Subcellular location	Cytoplasm HAMAP MF_00123.
Sequence similarities	Belongs to the class-I aminoacyl-tRNA synthetase family.

Ontologies

Keywords
Biological process	Protein biosynthesis
Cellular component	Cytoplasm
Ligand	ATP-binding Nucleotide-binding
Molecular function	Aminoacyl-tRNA synthetase Ligase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	arginyl-tRNA aminoacylation Inferred from electronic annotation. Source: InterPro
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW arginine-tRNA ligase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 600	600	Arginine--tRNA ligase HAMAP MF_00123		PRO_0000151595
Regions
	Motif	132 – 142	11	"HIGH" region HAMAP MF_00123

Sequences

	Sequence		Length	Mass (Da)
	Q8Y2P8 [UniParc]. Last modified March 1, 2002. Version 1. Checksum: E02008F9FA98355C	FASTA	600	65,085
	10 20 30 40 50 60 MLPSHKQTIS QLLSDAVGTL LPEGTNRPEI VLERPKQAAH GDIACNVALQ LAKPLGTNPR 70 80 90 100 110 120 ELANRIADGI RADARGQRLV SAVEIAGPGF INLRLSPTAR TDVLAAVFAE GDRYGAADLH 130 140 150 160 170 180 DGAPVLVEFV SANPTGPLHV GHGRQAALGD ALAALLEWQG HKVHREFYYN DAGVQIHNLA 190 200 210 220 230 240 VSVQARARGF KPGDTGWPEA AYNGDYIADI AADYLAGKTV RASDGEPVTG ARDVENIEAI 250 260 270 280 290 300 RRFAVTYLRN EQDIDLQAFG VKFDHYYLES SLYADGKVQQ TVDALIAAGK TYEQEGALWL 310 320 330 340 350 360 RTTDDGDDKD RVMRKSDGSY TYFVPDVAYH TTKWGRGFTQ VINVQGSDHH GTIARVRAGL 370 380 390 400 410 420 QGLDLGIPKG YPDYVLHKMV TVMKDGAEVK ISKRAGSYVT VRDLIEWSNG DAESEAGVDT 430 440 450 460 470 480 IRACVESGAP NWPGRFTRGR DAVRFFLLSR KADTEFVFDV DLALKQSDEN PVYYVQYAHA 490 500 510 520 530 540 RICSVFEQWH AREGGDAASL AGADLAAVAG PEASPQAVAL VQRIAAFPDM LADAARELAP 550 560 570 580 590 600 HAVAFYLRDL AGDFHAFYNA DRVLVDDDAV KRARLALLAA TRQVLRNGLA VIGVSAPQKM

References

[1]

"Genome sequence of the plant pathogen Ralstonia solanacearum." Salanoubat M., Genin S., Artiguenave F., Gouzy J., Mangenot S., Arlat M., Billault A., Brottier P., Camus J.-C., Cattolico L., Chandler M., Choisne N., Claudel-Renard C., Cunnac S., Demange N., Gaspin C., Lavie M., Moisan A. , Robert C., Saurin W., Schiex T., Siguier P., Thebault P., Whalen M., Wincker P., Levy M., Weissenbach J., Boucher C.A. Nature 415:497-502(2002) [PubMed: 11823852] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: GMI1000.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AL646052 Genomic DNA. Translation: CAD13815.1.
RefSeq	NP_518408.1. NC_003295.1.
3D structure databases
ProteinModelPortal	Q8Y2P8.
ModBase	Search...
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	1219090.
GenomeReviews	Gene locus RSc0287 in contig AL646052_GR.
KEGG	rso:RSc0287.
NMPDR	fig|267608.1.peg.287.
PATRIC	20259115. VBIRalSol70888_0292.
Organism-specific databases
CMR	Search...
Phylogenomic databases
HOGENOM	HBG695395.
OMA	ETIRGCL.
ProtClustDB	PRK01611.
Enzyme and pathway databases
BioCyc	RSOL267608:RSC0287-MONOMER.
Family and domain databases
HAMAP	MF_00123. Arg_tRNA_synth. [Tree]
InterPro	IPR001412. aa-tRNA-synth_I_CS. IPR001278. Arg-tRNA-synth_Ia. IPR015945. Arg-tRNA-synth_Ia_core. IPR005148. Arg-tRNA-synth_N. IPR008909. DALR_anticod-bd. IPR014729. Rossmann-like_a/b/a_fold. IPR009080. tRNAsynth_1a_anticodon-bd. [Graphical view]
Gene3D	G3DSA:3.30.1360.70. Arg-tRNA-synth_Ic_N. 1 hit. G3DSA:3.40.50.620. Rossmann-like_a/b/a_fold. 1 hit.
KO	K01887.
PANTHER	PTHR11956. Arg_tRNA-synt_1c. 1 hit.
Pfam	PF03485. Arg_tRNA_synt_N. 1 hit. PF05746. DALR_1. 1 hit. PF00750. tRNA-synt_1d. 1 hit. [Graphical view]
PRINTS	PR01038. TRNASYNTHARG.
SMART	SM01016. Arg_tRNA_synt_N. 1 hit. SM00836. DALR_1. 1 hit. [Graphical view]
SUPFAM	SSF55190. Arg-tRNA-synth_Ic_N. 1 hit. SSF47323. tRNAsyn_1a_bind. 1 hit.
TIGRFAMs	TIGR00456. ArgS. 1 hit.
PROSITE	PS00178. AA_TRNA_LIGASE_I. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

SYR_RALSO

Accession

Primary (citable) accession number: Q8Y2P8

Entry history

Integrated into UniProtKB/Swiss-Prot:	December 15, 2003
Last sequence update:	March 1, 2002
Last modified:	January 25, 2012
This is version 58 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program