Q8Y2E1 (KPRS_RALSO) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Ribose-phosphate pyrophosphokinase
Short name=RPPK
EC=2.7.6.1

Alternative name(s):
Phosphoribosyl pyrophosphate synthase
Short name=P-Rib-PP synthase
Short name=PRPP synthase

Gene names

Name:	prs
Synonyms:	prsA
Ordered Locus Names:	RSc0395
ORF Names:	RS03363

Organism

Ralstonia solanacearum (strain GMI1000) (Pseudomonas solanacearum) [Complete proteome] [HAMAP]

Taxonomic identifier

267608 [NCBI]

Taxonomic lineage

cellular organisms › Bacteria › Proteobacteria › Betaproteobacteria › Burkholderiales › Burkholderiaceae › Ralstonia › Ralstonia solanacearum

Protein attributes

Sequence length	316 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Catalytic activity	ATP + D-ribose 5-phosphate = AMP + 5-phospho-alpha-D-ribose 1-diphosphate. HAMAP-Rule MF_00583
Cofactor	Binds 1 magnesium ion per subunit By similarity.
Pathway	Metabolic intermediate biosynthesis; 5-phospho-alpha-D-ribose 1-diphosphate biosynthesis; 5-phospho-alpha-D-ribose 1-diphosphate from D-ribose 5-phosphate (route I): step 1/1. HAMAP-Rule MF_00583
Subcellular location	Cytoplasm By similarity.
Sequence similarities	Belongs to the ribose-phosphate pyrophosphokinase family.

Ontologies

Keywords
Biological process	Nucleotide biosynthesis
Cellular component	Cytoplasm
Ligand	ATP-binding Magnesium Metal-binding Nucleotide-binding
Molecular function	Kinase Transferase
Technical term	Complete proteome
Gene Ontology (GO)
Biological_process	5-phosphoribose 1-diphosphate biosynthetic process Inferred from electronic annotation. Source: UniProtKB-UniPathway nucleoside metabolic process Inferred from electronic annotation. Source: InterPro nucleotide biosynthetic process Inferred from electronic annotation. Source: UniProtKB-KW ribonucleoside monophosphate biosynthetic process Inferred from electronic annotation. Source: HAMAP
Cellular_component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular_function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW kinase activity Inferred from electronic annotation. Source: UniProtKB-KW magnesium ion binding Inferred from electronic annotation. Source: HAMAP ribose phosphate diphosphokinase activity Inferred from electronic annotation. Source: HAMAP
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 316	316	Ribose-phosphate pyrophosphokinase HAMAP-Rule MF_00583		PRO_0000141179
Regions
	Region	214 – 227	14	Binding of phosphoribosylpyrophosphate Potential
Sites
	Metal binding	131	1	Magnesium Potential
	Metal binding	133	1	Magnesium Potential
	Metal binding	142	1	Magnesium Potential
	Metal binding	146	1	Magnesium Potential

Sequences

	Sequence		Length	Mass (Da)
	Q8Y2E1 [UniParc]. Last modified March 1, 2002. Version 1. Checksum: 83613A8C824BD57C	FASTA	316	34,314
	10 20 30 40 50 60 MSSEGLMVFT GNANPKLAEA VVKHLNIPLG KALVGRFSDG EVQVEIQENV RGKHVFILQS 70 80 90 100 110 120 TCAPTNDNLM ELMVMVDALK RASARRITAA IPYFGYARQD RRPRSARVAI SAKVVANMLE 130 140 150 160 170 180 VAGVERVLTM DLHADQIQGF FDIPVDNIYA SPILLEDLRK KNYDNLLVVS PDVGGVVRAR 190 200 210 220 230 240 ALAKQLGVDL AIIDKRRPKA NVAEVMNIIG EVEGRNCVIM DDMIDTGGTL CKAAQVLKER 250 260 270 280 290 300 GAKQVFSYCT HPVLSGGAAA RIADSALDEV VVTDTIPLRD DAAQCGKIRQ LSTAPLLAET 310 FTRIVRGDSI MSLFAE

References

[1]

"Genome sequence of the plant pathogen Ralstonia solanacearum." Salanoubat M., Genin S., Artiguenave F., Gouzy J., Mangenot S., Arlat M., Billault A., Brottier P., Camus J.-C., Cattolico L., Chandler M., Choisne N., Claudel-Renard C., Cunnac S., Demange N., Gaspin C., Lavie M., Moisan A. , Robert C., Saurin W., Schiex T., Siguier P., Thebault P., Whalen M., Wincker P., Levy M., Weissenbach J., Boucher C.A. Nature 415:497-502(2002) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: GMI1000.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AL646052 Genomic DNA. Translation: CAD13923.1.
RefSeq	NP_518516.1. NC_003295.1.
3D structure databases
ProteinModelPortal	Q8Y2E1.
SMR	Q8Y2E1. Positions 5-311.
ModBase	Search...
Protein-protein interaction databases
STRING	267608.RSc0395.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblBacteria	CAD13923; CAD13923; RSc0395.
GeneID	1219198.
KEGG	rso:RSc0395.
PATRIC	20259335. VBIRalSol70888_0402.
Organism-specific databases
CMR	Search...
Phylogenomic databases
eggNOG	COG0462.
HOGENOM	HOG000210452.
KO	K00948.
OMA	TRISPTE.
ProtClustDB	PRK01259.
Enzyme and pathway databases
UniPathway	UPA00087; UER00172.
Family and domain databases
HAMAP	MF_00583_B. RibP_PPkinase_B.
InterPro	IPR000842. PRib_PP_synth_CS. IPR000836. PRibTrfase_dom. IPR005946. Rib-P_diPkinase. [Graphical view]
Pfam	PF00156. Pribosyltran. 1 hit. [Graphical view]
TIGRFAMs	TIGR01251. ribP_PPkin. 1 hit.
PROSITE	PS00114. PRPP_SYNTHASE. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

KPRS_RALSO

Accession

Primary (citable) accession number: Q8Y2E1

Entry history

Integrated into UniProtKB/Swiss-Prot:	October 25, 2002
Last sequence update:	March 1, 2002
Last modified:	May 1, 2013
This is version 74 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program