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Q8Y233 (RUVC_RALSO) Reviewed, UniProtKB/Swiss-Prot

Last modified May 14, 2014. Version 73. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (1) | Third-party data text xml rdf/xml gff fasta
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Names and origin

Protein namesRecommended name:
Crossover junction endodeoxyribonuclease RuvC

EC=3.1.22.4
Alternative name(s):
Holliday junction nuclease RuvC
Holliday junction resolvase RuvC
Gene names
Name:ruvC
Ordered Locus Names:RSc0503
ORF Names:RS05019
OrganismRalstonia solanacearum (strain GMI1000) (Pseudomonas solanacearum) [Complete proteome] [HAMAP]
Taxonomic identifier267608 [NCBI]
Taxonomic lineageBacteriaProteobacteriaBetaproteobacteriaBurkholderialesBurkholderiaceaeRalstonia

Protein attributes

Sequence length181 AA.
Sequence statusComplete.
Protein existenceInferred from homology

General annotation (Comments)

Function

Nuclease that resolves Holliday junction intermediates in genetic recombination. Cleaves the cruciform structure in supercoiled DNA by nicking to strands with the same polarity at sites symmetrically opposed at the junction in the homologous arms and leaves a 5'-terminal phosphate and a 3'-terminal hydroxyl group By similarity. HAMAP-Rule MF_00034

Catalytic activity

Endonucleolytic cleavage at a junction such as a reciprocal single-stranded crossover between two homologous DNA duplexes (Holliday junction). HAMAP-Rule MF_00034

Cofactor

Binds 1 magnesium ion per subunit By similarity. HAMAP-Rule MF_00034

Sequence similarities

Belongs to the RuvC family.

Ontologies

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 181181Crossover junction endodeoxyribonuclease RuvC HAMAP-Rule MF_00034
PRO_0000183124

Sites

Metal binding71Magnesium By similarity
Metal binding671Magnesium By similarity
Metal binding1391Magnesium By similarity
Metal binding1421Magnesium By similarity

Sequences

Sequence LengthMass (Da)Tools
Q8Y233 [UniParc].

Last modified March 1, 2002. Version 1.
Checksum: 180F7E04EB305EAC

FASTA18119,260
        10         20         30         40         50         60 
MRILGIDPGL RTTGFGVLER HGHQLVYVAS GTIKSDGNAD LPSRLKTLYD GVSELVRTYR 

        70         80         90        100        110        120 
PDCASIEKVF VNVNPQSTLL LGQARGAVIC GLMSGNLPVF EYTALQLKQA VVGYGRANKD 

       130        140        150        160        170        180 
QVQDMVVRLL NLEGRPGTDA ADALGVAICH AHGGETLAAM AGLAPQLARK GLRVRRGRLV 


G 

« Hide

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
AL646052 Genomic DNA. Translation: CAD14031.1.
RefSeqNP_518624.1. NC_003295.1.

3D structure databases

ProteinModelPortalQ8Y233.
SMRQ8Y233. Positions 2-155.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

STRING267608.RSc0503.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaCAD14031; CAD14031; RSc0503.
GeneID1219307.
KEGGrso:RSc0503.
PATRIC20259581. VBIRalSol70888_0524.

Organism-specific databases

CMRSearch...

Phylogenomic databases

eggNOGCOG0817.
HOGENOMHOG000012181.
KOK01159.
OMAPDCAAIE.
OrthoDBEOG6RG044.

Enzyme and pathway databases

BioCycRSOL267608:GCVU-505-MONOMER.

Family and domain databases

Gene3D3.30.420.10. 1 hit.
HAMAPMF_00034. RuvC.
InterProIPR012337. RNaseH-like_dom.
IPR020563. X-over_junc_endoDNase_Mg_BS.
IPR002176. X-over_junc_endoDNase_RuvC.
[Graphical view]
PfamPF02075. RuvC. 1 hit.
[Graphical view]
PRINTSPR00696. RSOLVASERUVC.
SUPFAMSSF53098. SSF53098. 1 hit.
TIGRFAMsTIGR00228. ruvC. 1 hit.
PROSITEPS01321. RUVC. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameRUVC_RALSO
AccessionPrimary (citable) accession number: Q8Y233
Entry history
Integrated into UniProtKB/Swiss-Prot: May 15, 2002
Last sequence update: March 1, 2002
Last modified: May 14, 2014
This is version 73 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families