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Q8Y1I7

- TRMB_RALSO

UniProt

Q8Y1I7 - TRMB_RALSO

Protein

tRNA (guanine-N(7)-)-methyltransferase

Gene

trmB

Organism
Ralstonia solanacearum (strain GMI1000) (Pseudomonas solanacearum)
Status
Reviewed - Annotation score: 2 out of 5- Protein inferred from homologyi
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    • History
      Entry version 71 (01 Oct 2014)
      Sequence version 1 (01 Mar 2002)
      Previous versions | rss
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    Functioni

    Catalyzes the formation of N(7)-methylguanine at position 46 (m7G46) in tRNA.UniRule annotation

    Catalytic activityi

    S-adenosyl-L-methionine + guanine(46) in tRNA = S-adenosyl-L-homocysteine + N(7)-methylguanine(46) in tRNA.UniRule annotation

    Pathwayi

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Binding sitei82 – 821S-adenosyl-L-methionineUniRule annotation
    Binding sitei107 – 1071S-adenosyl-L-methionineUniRule annotation
    Binding sitei134 – 1341S-adenosyl-L-methionineUniRule annotation
    Active sitei157 – 1571By similarity
    Binding sitei157 – 1571S-adenosyl-L-methionineUniRule annotation
    Binding sitei161 – 1611SubstrateUniRule annotation
    Binding sitei193 – 1931SubstrateUniRule annotation

    GO - Molecular functioni

    1. tRNA (guanine-N7-)-methyltransferase activity Source: UniProtKB-HAMAP

    Keywords - Molecular functioni

    Methyltransferase, Transferase

    Keywords - Biological processi

    tRNA processing

    Keywords - Ligandi

    S-adenosyl-L-methionine

    Enzyme and pathway databases

    BioCyciRSOL267608:GCVU-706-MONOMER.
    UniPathwayiUPA00989.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    tRNA (guanine-N(7)-)-methyltransferaseUniRule annotation (EC:2.1.1.33UniRule annotation)
    Alternative name(s):
    tRNA (guanine(46)-N(7))-methyltransferaseUniRule annotation
    tRNA(m7G46)-methyltransferaseUniRule annotation
    Gene namesi
    Name:trmBUniRule annotation
    Ordered Locus Names:RSc0703
    ORF Names:RS01608
    OrganismiRalstonia solanacearum (strain GMI1000) (Pseudomonas solanacearum)
    Taxonomic identifieri267608 [NCBI]
    Taxonomic lineageiBacteriaProteobacteriaBetaproteobacteriaBurkholderialesBurkholderiaceaeRalstonia
    ProteomesiUP000001436: Chromosome

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 251251tRNA (guanine-N(7)-)-methyltransferasePRO_0000171379Add
    BLAST

    Interactioni

    Protein-protein interaction databases

    STRINGi267608.RSc0703.

    Structurei

    3D structure databases

    ProteinModelPortaliQ8Y1I7.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Region

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Regioni163 – 1686Interaction with RNAUniRule annotation
    Regioni228 – 2314Substrate bindingUniRule annotation

    Sequence similaritiesi

    Belongs to the class I-like SAM-binding methyltransferase superfamily. TrmB family.UniRule annotation

    Phylogenomic databases

    eggNOGiCOG0220.
    HOGENOMiHOG000073968.
    KOiK03439.
    OMAiHSTLEMA.
    OrthoDBiEOG6K6VBC.

    Family and domain databases

    Gene3Di3.40.50.150. 1 hit.
    HAMAPiMF_01057. tRNA_methyltr_TrmB.
    InterProiIPR029063. SAM-dependent_MTases-like.
    IPR003358. tRNA_(Gua-N-7)_MeTrfase.
    [Graphical view]
    PfamiPF02390. Methyltransf_4. 1 hit.
    [Graphical view]
    SUPFAMiSSF53335. SSF53335. 1 hit.
    TIGRFAMsiTIGR00091. TIGR00091. 1 hit.
    PROSITEiPS51625. SAM_MT_TRMB. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    Q8Y1I7-1 [UniParc]FASTAAdd to Basket

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    MQPNEQPGTG PADTTLEQQD TAAAEVGHPR RIRSFVRRAG RTSTGQQRAI    50
    DELGPRFLLP YAAAPLDWEA AFGRTGARRI FEIGFGMGET SAHIAQLRPD 100
    DDFLGVEVHE PGVGALLKLI GERGIGNVRI VSHDAVEVLA QMIPEGTLDG 150
    IHVFFPDPWH KKRHNKRRLI QGPFVARLAA HLRPGGYLHC ATDWEEYAHQ 200
    MLEVLSAEPL LENTADGFAP RPDYRPVTKF EKRGLRLGHG VWDVVFRKRA 250
    A 251
    Length:251
    Mass (Da):27,913
    Last modified:March 1, 2002 - v1
    Checksum:i311572881A3ED6B2
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AL646052 Genomic DNA. Translation: CAD14233.1.
    RefSeqiNP_518824.1. NC_003295.1.

    Genome annotation databases

    EnsemblBacteriaiCAD14233; CAD14233; RSc0703.
    GeneIDi1219508.
    KEGGirso:RSc0703.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AL646052 Genomic DNA. Translation: CAD14233.1 .
    RefSeqi NP_518824.1. NC_003295.1.

    3D structure databases

    ProteinModelPortali Q8Y1I7.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    STRINGi 267608.RSc0703.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblBacteriai CAD14233 ; CAD14233 ; RSc0703 .
    GeneIDi 1219508.
    KEGGi rso:RSc0703.

    Phylogenomic databases

    eggNOGi COG0220.
    HOGENOMi HOG000073968.
    KOi K03439.
    OMAi HSTLEMA.
    OrthoDBi EOG6K6VBC.

    Enzyme and pathway databases

    UniPathwayi UPA00989 .
    BioCyci RSOL267608:GCVU-706-MONOMER.

    Family and domain databases

    Gene3Di 3.40.50.150. 1 hit.
    HAMAPi MF_01057. tRNA_methyltr_TrmB.
    InterProi IPR029063. SAM-dependent_MTases-like.
    IPR003358. tRNA_(Gua-N-7)_MeTrfase.
    [Graphical view ]
    Pfami PF02390. Methyltransf_4. 1 hit.
    [Graphical view ]
    SUPFAMi SSF53335. SSF53335. 1 hit.
    TIGRFAMsi TIGR00091. TIGR00091. 1 hit.
    PROSITEi PS51625. SAM_MT_TRMB. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: GMI1000.

    Entry informationi

    Entry nameiTRMB_RALSO
    AccessioniPrimary (citable) accession number: Q8Y1I7
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: June 16, 2003
    Last sequence update: March 1, 2002
    Last modified: October 1, 2014
    This is version 71 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programProkaryotic Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. PATHWAY comments
      Index of metabolic and biosynthesis pathways
    2. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3