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Q8Y1I7 (TRMB_RALSO) Reviewed, UniProtKB/Swiss-Prot

Last modified April 16, 2014. Version 68. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
tRNA (guanine-N(7)-)-methyltransferase

EC=2.1.1.33
Alternative name(s):
tRNA (guanine(46)-N(7))-methyltransferase
tRNA(m7G46)-methyltransferase
Gene names
Name:trmB
Ordered Locus Names:RSc0703
ORF Names:RS01608
OrganismRalstonia solanacearum (strain GMI1000) (Pseudomonas solanacearum) [Complete proteome] [HAMAP]
Taxonomic identifier267608 [NCBI]
Taxonomic lineageBacteriaProteobacteriaBetaproteobacteriaBurkholderialesBurkholderiaceaeRalstonia

Protein attributes

Sequence length251 AA.
Sequence statusComplete.
Protein existenceInferred from homology

General annotation (Comments)

Function

Catalyzes the formation of N(7)-methylguanine at position 46 (m7G46) in tRNA By similarity. HAMAP-Rule MF_01057

Catalytic activity

S-adenosyl-L-methionine + guanine46 in tRNA = S-adenosyl-L-homocysteine + N(7)-methylguanine46 in tRNA. HAMAP-Rule MF_01057

Pathway

tRNA modification; N(7)-methylguanine-tRNA biosynthesis. HAMAP-Rule MF_01057

Sequence similarities

Belongs to the class I-like SAM-binding methyltransferase superfamily. TrmB family.

Ontologies

Keywords
   Biological processtRNA processing
   LigandS-adenosyl-L-methionine
   Molecular functionMethyltransferase
Transferase
   Technical termComplete proteome
Gene Ontology (GO)
   Molecular_functiontRNA (guanine-N7-)-methyltransferase activity

Inferred from electronic annotation. Source: UniProtKB-HAMAP

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 251251tRNA (guanine-N(7)-)-methyltransferase HAMAP-Rule MF_01057
PRO_0000171379

Regions

Region163 – 1686Interaction with RNA Potential
Region228 – 2314Substrate binding By similarity

Sites

Active site1571 By similarity
Binding site821S-adenosyl-L-methionine By similarity
Binding site1071S-adenosyl-L-methionine By similarity
Binding site1341S-adenosyl-L-methionine By similarity
Binding site1571S-adenosyl-L-methionine By similarity
Binding site1611Substrate By similarity
Binding site1931Substrate By similarity

Sequences

Sequence LengthMass (Da)Tools
Q8Y1I7 [UniParc].

Last modified March 1, 2002. Version 1.
Checksum: 311572881A3ED6B2

FASTA25127,913
        10         20         30         40         50         60 
MQPNEQPGTG PADTTLEQQD TAAAEVGHPR RIRSFVRRAG RTSTGQQRAI DELGPRFLLP 

        70         80         90        100        110        120 
YAAAPLDWEA AFGRTGARRI FEIGFGMGET SAHIAQLRPD DDFLGVEVHE PGVGALLKLI 

       130        140        150        160        170        180 
GERGIGNVRI VSHDAVEVLA QMIPEGTLDG IHVFFPDPWH KKRHNKRRLI QGPFVARLAA 

       190        200        210        220        230        240 
HLRPGGYLHC ATDWEEYAHQ MLEVLSAEPL LENTADGFAP RPDYRPVTKF EKRGLRLGHG 

       250 
VWDVVFRKRA A 

« Hide

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
AL646052 Genomic DNA. Translation: CAD14233.1.
RefSeqNP_518824.1. NC_003295.1.

3D structure databases

ProteinModelPortalQ8Y1I7.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

STRING267608.RSc0703.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaCAD14233; CAD14233; RSc0703.
GeneID1219508.
KEGGrso:RSc0703.

Organism-specific databases

CMRSearch...

Phylogenomic databases

eggNOGCOG0220.
HOGENOMHOG000073968.
KOK03439.
OMADNYWPVM.
OrthoDBEOG6K6VBC.
ProtClustDBPRK00121.

Enzyme and pathway databases

BioCycRSOL267608:GCVU-706-MONOMER.
UniPathwayUPA00989.

Family and domain databases

HAMAPMF_01057. tRNA_methyltr_TrmB.
InterProIPR003358. tRNA_(Gua-N-7)_MeTrfase.
[Graphical view]
PfamPF02390. Methyltransf_4. 1 hit.
[Graphical view]
TIGRFAMsTIGR00091. TIGR00091. 1 hit.
PROSITEPS51625. SAM_MT_TRMB. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameTRMB_RALSO
AccessionPrimary (citable) accession number: Q8Y1I7
Entry history
Integrated into UniProtKB/Swiss-Prot: June 16, 2003
Last sequence update: March 1, 2002
Last modified: April 16, 2014
This is version 68 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

PATHWAY comments

Index of metabolic and biosynthesis pathways