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Q8Y1I7

- TRMB_RALSO

UniProt

Q8Y1I7 - TRMB_RALSO

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Protein

tRNA (guanine-N(7)-)-methyltransferase

Gene

trmB

Organism
Ralstonia solanacearum (strain GMI1000) (Pseudomonas solanacearum)
Status
Reviewed - Annotation score: 2 out of 5- Protein inferred from homologyi

Functioni

Catalyzes the formation of N(7)-methylguanine at position 46 (m7G46) in tRNA.UniRule annotation

Catalytic activityi

S-adenosyl-L-methionine + guanine(46) in tRNA = S-adenosyl-L-homocysteine + N(7)-methylguanine(46) in tRNA.UniRule annotation

Pathwayi

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei82 – 821S-adenosyl-L-methionineUniRule annotation
Binding sitei107 – 1071S-adenosyl-L-methionineUniRule annotation
Binding sitei134 – 1341S-adenosyl-L-methionineUniRule annotation
Active sitei157 – 1571By similarity
Binding sitei157 – 1571S-adenosyl-L-methionineUniRule annotation
Binding sitei161 – 1611SubstrateUniRule annotation
Binding sitei193 – 1931SubstrateUniRule annotation

GO - Molecular functioni

  1. tRNA (guanine-N7-)-methyltransferase activity Source: UniProtKB-HAMAP
Complete GO annotation...

Keywords - Molecular functioni

Methyltransferase, Transferase

Keywords - Biological processi

tRNA processing

Keywords - Ligandi

S-adenosyl-L-methionine

Enzyme and pathway databases

BioCyciRSOL267608:GCVU-706-MONOMER.
UniPathwayiUPA00989.

Names & Taxonomyi

Protein namesi
Recommended name:
tRNA (guanine-N(7)-)-methyltransferaseUniRule annotation (EC:2.1.1.33UniRule annotation)
Alternative name(s):
tRNA (guanine(46)-N(7))-methyltransferaseUniRule annotation
tRNA(m7G46)-methyltransferaseUniRule annotation
Gene namesi
Name:trmBUniRule annotation
Ordered Locus Names:RSc0703
ORF Names:RS01608
OrganismiRalstonia solanacearum (strain GMI1000) (Pseudomonas solanacearum)
Taxonomic identifieri267608 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaBetaproteobacteriaBurkholderialesBurkholderiaceaeRalstonia
ProteomesiUP000001436: Chromosome

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 251251tRNA (guanine-N(7)-)-methyltransferasePRO_0000171379Add
BLAST

Interactioni

Protein-protein interaction databases

STRINGi267608.RSc0703.

Structurei

3D structure databases

ProteinModelPortaliQ8Y1I7.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni163 – 1686Interaction with RNAUniRule annotation
Regioni228 – 2314Substrate bindingUniRule annotation

Sequence similaritiesi

Belongs to the class I-like SAM-binding methyltransferase superfamily. TrmB family.UniRule annotation

Phylogenomic databases

eggNOGiCOG0220.
HOGENOMiHOG000073968.
KOiK03439.
OMAiHSTLEMA.
OrthoDBiEOG6K6VBC.

Family and domain databases

Gene3Di3.40.50.150. 1 hit.
HAMAPiMF_01057. tRNA_methyltr_TrmB.
InterProiIPR029063. SAM-dependent_MTases-like.
IPR003358. tRNA_(Gua-N-7)_MeTrfase.
[Graphical view]
PfamiPF02390. Methyltransf_4. 1 hit.
[Graphical view]
SUPFAMiSSF53335. SSF53335. 1 hit.
TIGRFAMsiTIGR00091. TIGR00091. 1 hit.
PROSITEiPS51625. SAM_MT_TRMB. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q8Y1I7-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MQPNEQPGTG PADTTLEQQD TAAAEVGHPR RIRSFVRRAG RTSTGQQRAI
60 70 80 90 100
DELGPRFLLP YAAAPLDWEA AFGRTGARRI FEIGFGMGET SAHIAQLRPD
110 120 130 140 150
DDFLGVEVHE PGVGALLKLI GERGIGNVRI VSHDAVEVLA QMIPEGTLDG
160 170 180 190 200
IHVFFPDPWH KKRHNKRRLI QGPFVARLAA HLRPGGYLHC ATDWEEYAHQ
210 220 230 240 250
MLEVLSAEPL LENTADGFAP RPDYRPVTKF EKRGLRLGHG VWDVVFRKRA

A
Length:251
Mass (Da):27,913
Last modified:March 1, 2002 - v1
Checksum:i311572881A3ED6B2
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AL646052 Genomic DNA. Translation: CAD14233.1.
RefSeqiNP_518824.1. NC_003295.1.
WP_011000658.1. NC_003295.1.

Genome annotation databases

EnsemblBacteriaiCAD14233; CAD14233; RSc0703.
GeneIDi1219508.
KEGGirso:RSc0703.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AL646052 Genomic DNA. Translation: CAD14233.1 .
RefSeqi NP_518824.1. NC_003295.1.
WP_011000658.1. NC_003295.1.

3D structure databases

ProteinModelPortali Q8Y1I7.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

STRINGi 267608.RSc0703.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblBacteriai CAD14233 ; CAD14233 ; RSc0703 .
GeneIDi 1219508.
KEGGi rso:RSc0703.

Phylogenomic databases

eggNOGi COG0220.
HOGENOMi HOG000073968.
KOi K03439.
OMAi HSTLEMA.
OrthoDBi EOG6K6VBC.

Enzyme and pathway databases

UniPathwayi UPA00989 .
BioCyci RSOL267608:GCVU-706-MONOMER.

Family and domain databases

Gene3Di 3.40.50.150. 1 hit.
HAMAPi MF_01057. tRNA_methyltr_TrmB.
InterProi IPR029063. SAM-dependent_MTases-like.
IPR003358. tRNA_(Gua-N-7)_MeTrfase.
[Graphical view ]
Pfami PF02390. Methyltransf_4. 1 hit.
[Graphical view ]
SUPFAMi SSF53335. SSF53335. 1 hit.
TIGRFAMsi TIGR00091. TIGR00091. 1 hit.
PROSITEi PS51625. SAM_MT_TRMB. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

  1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: GMI1000.

Entry informationi

Entry nameiTRMB_RALSO
AccessioniPrimary (citable) accession number: Q8Y1I7
Entry historyi
Integrated into UniProtKB/Swiss-Prot: June 16, 2003
Last sequence update: March 1, 2002
Last modified: October 29, 2014
This is version 72 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3