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Protein

Catalase-peroxidase

Gene

katG

Organism
Ralstonia solanacearum (strain GMI1000) (Pseudomonas solanacearum)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Protein inferred from homologyi

Functioni

Bifunctional enzyme with both catalase and broad-spectrum peroxidase activity.UniRule annotation

Catalytic activityi

Donor + H2O2 = oxidized donor + 2 H2O.UniRule annotation
2 H2O2 = O2 + 2 H2O.UniRule annotation

Cofactori

heme bUniRule annotationNote: Binds 1 heme b (iron(II)-protoporphyrin IX) group per dimer.UniRule annotation

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sitei89 – 891Transition state stabilizerUniRule annotation
Active sitei93 – 931Proton acceptorUniRule annotation
Metal bindingi256 – 2561Iron (heme axial ligand)UniRule annotation

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Oxidoreductase, Peroxidase

Keywords - Biological processi

Hydrogen peroxide

Keywords - Ligandi

Heme, Iron, Metal-binding

Enzyme and pathway databases

BioCyciRSOL267608:GCVU-780-MONOMER.
RSOL267608:GCVU-781-MONOMER.

Protein family/group databases

PeroxiBasei3065. RsoCP01_GMI1000.

Names & Taxonomyi

Protein namesi
Recommended name:
Catalase-peroxidaseUniRule annotation (EC:1.11.1.21UniRule annotation)
Short name:
CPUniRule annotation
Alternative name(s):
Peroxidase/catalaseUniRule annotation
Gene namesi
Name:katGUniRule annotation
Ordered Locus Names:RSc0776/RSc0775
OrganismiRalstonia solanacearum (strain GMI1000) (Pseudomonas solanacearum)
Taxonomic identifieri267608 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaBetaproteobacteriaBurkholderialesBurkholderiaceaeRalstonia
Proteomesi
  • UP000001436 Componenti: Chromosome

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 724724Catalase-peroxidasePRO_0000354880Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Cross-linki92 ↔ 215Tryptophyl-tyrosyl-methioninium (Trp-Tyr) (with M-241)UniRule annotation
Cross-linki215 ↔ 241Tryptophyl-tyrosyl-methioninium (Tyr-Met) (with W-92)UniRule annotation

Post-translational modificationi

The covalent Trp-Tyr-Met adduct is important for the catalase, but not the peroxidase activity of the enzyme.UniRule annotation

Proteomic databases

PRIDEiQ8Y1B4.

Interactioni

Subunit structurei

Homodimer or homotetramer.UniRule annotation

Protein-protein interaction databases

STRINGi267608.RSc0775.

Family & Domainsi

Sequence similaritiesi

Belongs to the peroxidase family. Peroxidase/catalase subfamily.UniRule annotation

Phylogenomic databases

eggNOGiENOG4105C1X. Bacteria.
COG0376. LUCA.
KOiK03782.
OrthoDBiPOG091H05R1.

Family and domain databases

HAMAPiMF_01961. Catal_peroxid. 1 hit.
InterProiIPR000763. Catalase_peroxidase.
IPR010255. Haem_peroxidase.
IPR002016. Haem_peroxidase_pln/fun/bac.
IPR019794. Peroxidases_AS.
IPR019793. Peroxidases_heam-ligand_BS.
[Graphical view]
PfamiPF00141. peroxidase. 2 hits.
[Graphical view]
PRINTSiPR00460. BPEROXIDASE.
PR00458. PEROXIDASE.
SUPFAMiSSF48113. SSF48113. 2 hits.
TIGRFAMsiTIGR00198. cat_per_HPI. 1 hit.
PROSITEiPS00435. PEROXIDASE_1. 1 hit.
PS00436. PEROXIDASE_2. 1 hit.
PS50873. PEROXIDASE_4. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q8Y1B4-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MTTESKCPFK HAAAGGGVSN RDWWPNQLNL KVLHQHSSKS DPMDTDFDYA
60 70 80 90 100
QAFKRLDLAA VKQDLLALMT TSQDWWPADF GHYGPLFIRM AWHSAGTYRT
110 120 130 140 150
GDGRGGAGAG QQRFAPLNSW PDNANLDKAR RLLWPIKQKY GRNISWADLM
160 170 180 190 200
ILTGNVALES MGFKTFGFAG GRKDVWEPEE DVYWGSETTW LGDQRYTGDR
210 220 230 240 250
DLENPLAAVQ MGLIYVNPEG PNGNPDPIAA ARDIRETFAR MAMNDEETVA
260 270 280 290 300
LIAGGHSFGK THGAGPAPTW GPEPEAAGIE EQGLGWSSRF GTGKGTDAIT
310 320 330 340 350
SGLEVTWTTT PTQWSNNFFQ NLFGYEWELT KSPAGAHQWV AKDAAETIPD
360 370 380 390 400
AHDPSAKHLP TMLTTDLSLR FDPAYEKISR RFYEHPEQFA DAFARAWFKL
410 420 430 440 450
THRDMGPRAR YLGPEVPAEA LIWQDPIPAV DHKLIDTQDI AALKARILAS
460 470 480 490 500
GLSVSQLVST AWASASTFRG SDMRGGANGA RIRLAPQKDW EANQPAQLAK
510 520 530 540 550
VLETLESIQG AFNGAQSGGK KVSLADLIVL AGCAGVEQAA KNAGHAVEVP
560 570 580 590 600
FTPGRMDAAQ AQTDVESFAV LEPIADGFRN YQKGKYTLPA EALLVDKAQL
610 620 630 640 650
LTLTAPEMTV LVGGLRVLDT NIGQTRHGVF TQRPESLTND FFVNLLDMGT
660 670 680 690 700
EWKATDGRDV FEGRDRATGA LKWTGTRVDL VFGSHSQLRA LAEVYGSADA
710 720
QAKFVRDFVA AWDKVMNLDR FDLA
Length:724
Mass (Da):79,362
Last modified:November 25, 2008 - v2
Checksum:i859F125FCEBB1DA1
GO

Sequence cautioni

The sequence CAD14477 differs from that shown. Reason: Frameshift at position 271. Curated
The sequence CAD14478 differs from that shown. Reason: Frameshift at position 271. Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AL646052 Genomic DNA. Translation: CAD14477.1. Frameshift.
AL646052 Genomic DNA. Translation: CAD14478.1. Frameshift.

Genome annotation databases

EnsemblBacteriaiCAD14477; CAD14477; RSc0775.
CAD14478; CAD14478; RSc0776.
KEGGirso:RSc0775.
rso:RSc0776.
PATRICi20260149. VBIRalSol70888_0806.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AL646052 Genomic DNA. Translation: CAD14477.1. Frameshift.
AL646052 Genomic DNA. Translation: CAD14478.1. Frameshift.

3D structure databases

ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi267608.RSc0775.

Protein family/group databases

PeroxiBasei3065. RsoCP01_GMI1000.

Proteomic databases

PRIDEiQ8Y1B4.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiCAD14477; CAD14477; RSc0775.
CAD14478; CAD14478; RSc0776.
KEGGirso:RSc0775.
rso:RSc0776.
PATRICi20260149. VBIRalSol70888_0806.

Phylogenomic databases

eggNOGiENOG4105C1X. Bacteria.
COG0376. LUCA.
KOiK03782.
OrthoDBiPOG091H05R1.

Enzyme and pathway databases

BioCyciRSOL267608:GCVU-780-MONOMER.
RSOL267608:GCVU-781-MONOMER.

Family and domain databases

HAMAPiMF_01961. Catal_peroxid. 1 hit.
InterProiIPR000763. Catalase_peroxidase.
IPR010255. Haem_peroxidase.
IPR002016. Haem_peroxidase_pln/fun/bac.
IPR019794. Peroxidases_AS.
IPR019793. Peroxidases_heam-ligand_BS.
[Graphical view]
PfamiPF00141. peroxidase. 2 hits.
[Graphical view]
PRINTSiPR00460. BPEROXIDASE.
PR00458. PEROXIDASE.
SUPFAMiSSF48113. SSF48113. 2 hits.
TIGRFAMsiTIGR00198. cat_per_HPI. 1 hit.
PROSITEiPS00435. PEROXIDASE_1. 1 hit.
PS00436. PEROXIDASE_2. 1 hit.
PS50873. PEROXIDASE_4. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiKATG_RALSO
AccessioniPrimary (citable) accession number: Q8Y1B4
Secondary accession number(s): Q8Y1B5
Entry historyi
Integrated into UniProtKB/Swiss-Prot: November 25, 2008
Last sequence update: November 25, 2008
Last modified: September 7, 2016
This is version 90 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.