Q8Y199 (SYQ_RALSO) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Glutamine--tRNA ligase
EC=6.1.1.18

Alternative name(s):
Glutaminyl-tRNA synthetase
Short name=GlnRS

Gene names

Name:	glnS
Ordered Locus Names:	RSc0791
ORF Names:	RS05046

Organism

Ralstonia solanacearum (strain GMI1000) (Pseudomonas solanacearum) [Complete proteome] [HAMAP]

Taxonomic identifier

267608 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Betaproteobacteria › Burkholderiales › Burkholderiaceae › Ralstonia

Protein attributes

Sequence length	580 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Catalytic activity	ATP + L-glutamine + tRNA(Gln) = AMP + diphosphate + L-glutaminyl-tRNA(Gln). HAMAP MF_00126
Subunit structure	Monomer By similarity. HAMAP MF_00126
Subcellular location	Cytoplasm HAMAP MF_00126.
Sequence similarities	Belongs to the class-I aminoacyl-tRNA synthetase family.

Ontologies

Keywords
Biological process	Protein biosynthesis
Cellular component	Cytoplasm
Ligand	ATP-binding Nucleotide-binding
Molecular function	Aminoacyl-tRNA synthetase Ligase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	glutaminyl-tRNA aminoacylation Inferred from electronic annotation. Source: InterPro
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW glutamine-tRNA ligase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 580	580	Glutamine--tRNA ligase HAMAP MF_00126		PRO_0000195846
Regions
	Motif	51 – 61	11	"HIGH" region HAMAP MF_00126
	Motif	294 – 298	5	"KMSKS" region HAMAP MF_00126
Sites
	Binding site	297	1	ATP By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q8Y199 [UniParc]. Last modified March 1, 2002. Version 1. Checksum: AA6C3A8783EA769C	FASTA	580	65,607
	10 20 30 40 50 60 MSQDNATGAA AASTSNFLRQ IIDTDLEQGT YAGRQDTAGH ALPPIITRFP PEPNGYLHIG 70 80 90 100 110 120 HAKSIWVNFG LAKEYGGRCH LRFDDTNPVK EDTEYVDSII DAVHWLGYSW QNGTGEHLYY 130 140 150 160 170 180 ASDYFEQLYG FAEVLIQRGA AYIDSQSAEQ IAANRGDFTR PGTPSPFRDR SVEENLALFR 190 200 210 220 230 240 DMRAGKYQDG QHVLRARIDM AAPNIVMRDP VLYRIRHAHH HRTGDAWCIY PMYDFTHCIS 250 260 270 280 290 300 DALENITHSL CTLEFENNRP LYDWVLDHLR DAGALPAPLP HQYEFARLHL TYAITSKRKL 310 320 330 340 350 360 LQLVNEKRVD GWDDPRMPTL VGIRRRGYTP ESIQLFCERV GVSKADSWID MSILEAAVRD 370 380 390 400 410 420 DLDARAPRSV AVLDPVKLIL DNVPADFNEP CSAPVHPKQP ELGRREFPLT RELWIEREDF 430 440 450 460 470 480 TETPPKGYFR LFPGNKVRLR YGYVIECTGC DKDADGNITA VHANIIPDTK SGTPGADSVK 490 500 510 520 530 540 VKGNIHWVSA AHALEAEVRL YDRLFTDPQP DSGDKNFLDA LNPDAKRVVT AYLEPTLATA 550 560 570 580 KPEDRFQFER HGYFVADRID SQPGKPVFNR VVGLKDSWGK

References

[1]

"Genome sequence of the plant pathogen Ralstonia solanacearum." Salanoubat M., Genin S., Artiguenave F., Gouzy J., Mangenot S., Arlat M., Billault A., Brottier P., Camus J.-C., Cattolico L., Chandler M., Choisne N., Claudel-Renard C., Cunnac S., Demange N., Gaspin C., Lavie M., Moisan A. , Robert C., Saurin W., Schiex T., Siguier P., Thebault P., Whalen M., Wincker P., Levy M., Weissenbach J., Boucher C.A. Nature 415:497-502(2002) [PubMed: 11823852] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: GMI1000.

+

Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AL646052 Genomic DNA. Translation: CAD14493.1.
RefSeq	NP_518912.1. NC_003295.1.
3D structure databases
ProteinModelPortal	Q8Y199.
SMR	Q8Y199. Positions 15-577.
ModBase	Search...
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	1219599.
GenomeReviews	Gene locus RSc0791 in contig AL646052_GR.
KEGG	rso:RSc0791.
NMPDR	fig|267608.1.peg.791.
PATRIC	20260185. VBIRalSol70888_0823.
Organism-specific databases
CMR	Search...
Phylogenomic databases
HOGENOM	HBG334108.
OMA	RMPTIAG.
ProtClustDB	PRK05347.
Enzyme and pathway databases
BioCyc	RSOL267608:RSC0791-MONOMER.
Family and domain databases
HAMAP	MF_00126. Gln_tRNA_synth. [Tree]
InterPro	IPR001412. aa-tRNA-synth_I_CS. IPR004514. Gln-tRNA-synth_Ib. IPR022861. Gln_tRNA_synth_bac. IPR000924. Glu/Gln-tRNA-synth_Ib. IPR020061. Glu/Gln-tRNA-synth_Ib_a-bdl. IPR020058. Glu/Gln-tRNA-synth_Ib_cat-dom. IPR020059. Glu/Gln-tRNA-synth_Ib_codon-bd. IPR020056. Rbsml_L25/Gln-tRNA_synth_b-brl. IPR011035. Ribosomal_L25/Gln-tRNA_synth. IPR014729. Rossmann-like_a/b/a_fold. [Graphical view]
Gene3D	G3DSA:1.10.1160.10. Glu/Gln-tRNA-synth_Ic_a-bdl. 1 hit. G3DSA:2.40.240.10. Rbsml_L25/Gln-tRNA_synth_b-brl. 2 hits. G3DSA:3.40.50.620. Rossmann-like_a/b/a_fold. 2 hits.
KO	K01886.
PANTHER	PTHR10119. Glu_tRNA-synt_1c. 1 hit.
Pfam	PF00749. tRNA-synt_1c. 1 hit. PF03950. tRNA-synt_1c_C. 1 hit. [Graphical view]
PRINTS	PR00987. TRNASYNTHGLU.
SUPFAM	SSF50715. Ribosomal_L25rel. 1 hit.
TIGRFAMs	TIGR00440. GlnS. 1 hit.
PROSITE	PS00178. AA_TRNA_LIGASE_I. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

SYQ_RALSO

Accession

Primary (citable) accession number: Q8Y199

Entry history

Integrated into UniProtKB/Swiss-Prot:	August 2, 2002
Last sequence update:	March 1, 2002
Last modified:	January 25, 2012
This is version 61 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program